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      • KCI등재

        노견(老犬)에 발생(發生)한 질섬유종(膣纖維腫)의 외과수술(外科手術) 1예(例)

        김명철,김교준,김덕환,조성환,권오덕,Kim, Myung Cheol,Kim, Kyo Joon,Kim, Duck Hwan,Cho, Sung Whan,Kwon, Oh Deok 충남대학교 농업과학연구소 1987 Korean Journal of Agricultural Science Vol.14 No.2

        질내(膣內)에 발생(發生)한 종양(腫瘍)으로 인하여 내원(來院)한 13년생(年生) terrier견(犬)에 대하여 회음부(會陰部)를 통한 종탕절제술(腫蕩切除術)을 실시(實施)한 증례(症例)를 보고(報告)한다. 1. 조직학적(組織學的)으로 관찰(觀察)한 결과 섬유종(纖維腫)으로 인정(認定)되었다. 2. 외음순(外陰脣)의 배측연접(背側連接) 상부(上部)의 1cm 폭(幅)을 남겨놓고 질부(膣部)의 배벽(背壁)까지 피부(皮膚) 및 근층(筋層)을 절개(切開)한 후절개창공(後切開創孔)을 통하여 종양물(腫瘍物)들을 절제(切除)한 결과(結果) 외음부(外陰部)의 형태에 변화(變化)를 일으키지 않고 원상(原狀)을 유지(維持)하였다. 3. 절제후(切除後) 현재(現在)까지 (약(約)6개월(個月)) 재발미후(再發微候)는 인정(認定)되지 않았다. A 13 years old terrior dog with vaginal tumor was referred to the animal hospital of Chungnam National University. Resection of vaginal tumor through perineal area was performed. The results obtained were as follows. 1. Histologically, tumor was classified with fibroma. 2. The skin and musculature of the perineal area was incised to the dorsal area of the vaginal wall except I em of width over the dorsal commissure of the vulvar cleft. Through the opening of the wound, pedunculated tumors were amputated and diffused tumors were removed by submucosal resection. After the surgical operation, the original shape of the external area of vulva was not changed. 3. After the resection recurrent sign was not observed up to nowadays for about 6 months.

      • SCOPUSKCI등재

        고활력우정자(高活力牛精子)의 선택적(選擇的) 분리(分離)에 관한 연구(硏究)

        김명철,Kim, Myung-cheol 대한수의학회 1984 大韓獸醫學會誌 Vol.24 No.2

        As a fundamental study to increase the reproductive efficiency in cattle, highly motile sperm were separated and collected from raw semen, extended semen and frozen semen by different methods using various concentrations of bovine serum albumin or Tyrode's solution. Various characteristics and light microscopic and electron-microscopic morphology of sperm separated by different methods were compared. The results obtained were as follows; 1. The sperm separated from raw semen using bovine serum albumin showed significantly high value in motility, motile sperm count, percent of normal sperm and progressive motility, as compared with control sperm and revealed the highest sperm recovery rate when separated with 6% bovine serum albumin. 2. The sperm motility, percent of normal sperm and progressive motility of the highly motile sperm frozen after being separated from raw semen with bovine serum albumin, showed significantly high value than those of a control sperm and especially found the highest value when separated with 20% bovine serum albumin. 3. Light-microscopic percent of abnormality was significantly low in the prefrozen and postfrozen highly motile sperm separated with bovine serum albumin, as compared with control sperm. 4. Electron-microscopic finding of the highly motile sperm separated with bovine serum albumin showed low percent of deformity in the dilatation and vesiculation of cell membrane, in dilatation and density loss of acrosome than in those of control sperm. 5. It was impossible to separate the highly motile sperm from frozen semen with bovine serum albumin, but it was possible with Tyrode's solution. 6. Recovery rate of highly motile sperm from raw semen extended semen and frozen semen was the highest when the sperm pellet stood in Tyrde's solution for 80 minutes. 7. The highly motile sperm separated from raw semen, extended semen and frozen semen with Tyrode's solution showed significantly high value in motility, progressive motility and percent of normal sperm, as compared with control sperm. 8. Highly motile sperm, when separated from raw semen, extended semen and frozen semen with Tyrode's solution, showed significantly low percent of microscopic abnormality as compared with control sperm.

      • SCOPUSKCI등재
      • SCOPUSKCI등재

        정자형태 및 정자농도의 검사를 위한 Unopette의 사용

        김명철,Kim Myung-Cheol 한국임상수의학회 1990 한국임상수의학회지 Vol.7 No.2

        This study was carried out determine whether Unopette can be used for the observation of sperm morphology and sperm Concentration. Rabbit sperm and frozen-thawed bovine sperm were observed with phase contrast microscope after dilution with Unopette acooriding to duration of preservation at 3~5$^{\circ}C$. Sperm using Unopette showed high normal sperm(%) than sperm using hematoxylin-eosin until 48 hours. Sperm using Unopette revealed no difference in sperm concentration until 24 hours, as compared with control sperm. As a result, Unopette was assessed as appropriate solution for preservation in terms of morphological observation and sperm concentration.

      • KCI등재

        알부민 구배(勾配) 정자분리(精子分離)에 의한 가토(家兎)의 성선택(性選擇) 시도(試圖)

        김명철,전무형,김교준,이규승,조성환,권오덕,이헌준,Kim, Myung Cheol,Jun, Moo Hyung,Kim, Kyo Joon,Lee, Kyu Seung,Cho, Sung Whan,Kwon, Oh Deog,Lee, Hun Jun 충남대학교 농업과학연구소 1987 Korean Journal of Agricultural Science Vol.14 No.2

        토끼에서 bovine serum albumin gradients로 성(性)의 전선택(前選擇)이 가능(可能)한지를 알아보기 위하여 6%, 10% 및 20%의 농도(濃度)를 달리한 bovine serum albumin을 사용한 방법(方法)으로 고활력정자(高活力精子)를 분리수집하여 정자(精子)의 성상(性狀)과 분리된 정액(精液)의 인공수정시(人工授精時)에 있어서 자토(仔兎)의 성비(性比)를 비교관찰한 결과는 다음과 같다. 1. Bovine serum albumin을 사용하여 분리(分離)된 정자(精子)는 임신율(妊娠率)에 있어서는 대조군에 비해 높은 성적(成績)을 나타내었으나, 자토(仔兎)의 성비(性比)에 있어서는 변화를 일으키지 못하였다. 2. Bovine serum albumin에 의해 분리(分離)된 정자(精子)는 대조군에 비하여 운동성(運動性), 정상정자율(正常精子率) 및 전진운동성(前進運動性)이 현저히 높았으며, 정자회수율(精子回數率)은 6%일 때 가장 높았다. 3. 원정액(原精液)으로부터 bovine serum albumin을 사용하여 고활력정자(高活力精子)를 분리(分離)한 후(後) 냉동(冷凍)한 정액(精液)의 융해후(融解後) 정자운동성(精子運動性), 정상정자율(正常精子率) 및 전진운동성(前進運動性)은 대조군에 비하여 현저히 높았다. The objective of this study was to evaluate the possibility of sex preselection by gradients methods using bovine serum albumin in rabbits. Artificial insemination with separated sperm was performed, after highly motile sperm were separated by different methods using 6%, 10% and 20% bovine serum albumin. Various characteristics of separated sperm, and the conception rate and secondary sex ratio at artificial insemination with sperm separated by different methods were compared. The results obtained were as follows. 1. The conception rate of sperm separated by bovine serum albumin gradients was higher than th at of control sperm. But secondary sex ratio was not altered by this methods. 2. The sperm separated by bovine serum albumin gradients showed significantly high value in motility, percent of normal sperm and progressive motility as compared with control sperm and revealed the highest sperm recovery when separated with 6% bovine serum albumin. 3. The sperm motility, percent of normal sperm and progressive motility of the highly motile sperm frozen after being separated from raw semen with bovine serum albumin, showed significantly high value than those of control sperm.

      • SCOPUSKCI등재

        한국산 잡견에서 신장의 초음파검사법

        김명철,박관호,박종오,김영범,Kim Myung-Cheol,Park Kwan-Ho,Park Chong-O,Kim Young-Beom 한국임상수의학회 1993 한국임상수의학회지 Vol.10 No.2

        Ultrasonography observations were made of the kidneys of 21 dogs. Ultrasonography images obtained in transverse and longitudinal planes. A 3.5-MHz linear transducer was used. All examinations were peformed on dogs in dorsal recumbency under anesthesia. The right kidney was 3.2~3.8cm long, 1.7~3.5 cm wide and 1.0~1.9cm deep in 0~5 of body weight group, 3.6~4.6cm long, 2.4~3.6cm wide and 1.4~2.1cm deep in 5~10 of body weight group, and 4.6~6.4cm long, 3.3~4.2cm wide and 2.0~2.0cm deep in 10~15kg of body weight group(p< 0.01). Diameter of the parenchyma of the right kidney ranged between 0.4~0.9cm in 0~5kg group, 0.6~1.0cm in 5~l0kg group and 0.9-1.1cm in 10~15kg group(p<0.01). Diameter of the renal sinus of the right kidney ranged between 0.3~0.5cm in 0~5kg group, 0.4~0.7cm in 5~10kg group and 0.5~0.9cm in 10~ 15kg group(p< 0.01). Similar ultrasonic measurements were obtained for the left kidney. It was concluded that the ultrasonographic findings described in this study can be used as references for diagnosis of morphologic changes in e canine kidney.

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