육군자탕의 정제 개발과 성분함량 및 약리효과 평가

김명진 ( Myoung-jin Kim ),최혜민 ( Hye-min Choi ),유병우 ( Byung-woo Yu ),홍영주 ( Young-ju Hong ),라채숙 ( Chae-suk Ra ),김민주 ( Min-ju Kim ),김정옥 ( Jung-ok Kim ) 대한본초학회 2021 大韓本草學會誌 Vol.36 No.1

Objective : Yukgunja-tang is one of the herbal prescriptions widely used for functional indigestion. In this study, we evaluated the pharmacological effect through the Yukgunja-tang formulation development. Methods : The RAW 264.7 cells were pretreated with Yukgunja-tang tablet (YGJT-T : 50, 100 and 200 ㎍/㎖) and then stimulated with lipopolysaccharide (LPS : 500 ng/㎖). Cell viability, inflammatory mediators such as nitric oxide (NO) and prostaglandin E2 (PGE2) and inflammatory cytokines (IL-1β, IL-6, and TNF-α) were measured. Also, ICR mice induced acute gastritis by oral administration of 150 mM HCl in 60% ethanol. The YGJT-T (30 ㎎/㎏) was pretreated for 3 days, and 150 mM HCl in 60% ethanol was orally administered 1 hour after the last drug treatment. Mice were sacrificed 1 hour after oral administration of 150 mM HCl in 60% ethanol. The gastric mucosa was observed, and inflammatory cytokines in the gastric tissue were measured. Results : The marker components of YGJT-T were determined by simultaneous analysis using HPLC. In RAW 264.7 cells, pretreatment of YGJT-T was non-toxic and inhibited the production of inflammatory mediators such as NO and PGE2 and suppressed inflammatory cytokines. In addition, pretreatment of YGJT-T improved bleeding and edema due to gastric lesions caused by acute gastritis and suppressed inflammatory cytokines. Conclusion : In summary, our results confirmed that treatment with YGJT-T has anti-inflammatory and anti-gastritis effects in vitro and in vivo. Therefore, in this study, YGJT-T could support a potential strategy for the prevention and treatment of gastritis.

다양한 각도의 환형관내 시추 유동에 대한 암편 이송 특성 연구

김명진(Young-Ju Kim),김경수(Kyung-Su Kim),이왕도(Wang-Do Lee),김태우(Tea-Woo Kim),한상목(Sang-Mok Han),우남섭(Nam-Sub Woo),김영주(Young-Ju Kim) 대한기계학회 2020 대한기계학회 춘추학술대회 Vol.2020 No.12

Cuttings transport and hole cleaning is important for an effective well drilling operation. And in directional drilling, it is very important to understand the drilling multi-phase flow characteristics because it is difficult to control cuttings transport. Therefore, various researches have been steadily conducted for efficient drilling technology development and field application, and many papers on drilling flow have been published. API developed Carrying Capacity Index(CCI) for describe hole cleaning also recommend an analytical and numerical approach. In this study, two empirical equations of Larsen’s model and Rubiandini’s model for the cuttings piece transfer velocity were applied to the numerical analysis technique. Through the two models, the minimum fluid velocity at which the cuttings in the inclined annular are not stagnant and can be transported was calculated and compared with the numerical analysis results. This simulation was performed with the DDPM + DEM model, and it is expected that it can be used to establish a safer and more effective drilling plan in a new drilling environment in the future.

하악골에 발생한 구강암 절제술후 대흉근 근피판을 이용한 즉시 재건술 : 증례보고 2예

김명진(Kim Myung jin),황인대(Hwang In Dae),이종호(Lee Jong Ho),이대경(Lee Dae Kyoung),최진영(Choi Jin Young),전주홍(Jeon Ju Hong),김종원(Kim Jong Won) 대한구강악안면외과학회 1987 대한구강악안면외과학회지 Vol.13 No.1

Surgical treatment of advanced carcinomas of the head and neck area often requires extensive resection, necessitating large flap for reconstruction. Although various myocutaneous flap which provide both muscle bulk and skin coverage is used for head and neck reconstruction, pectoralis major myocutaneous flap is used most frequently because of excellent vascularity, anatomic proximity, long arc of rotation, reliability and versatility. In this paper we present our experience of 2 cases of pectoralis major myocutaneous flap used in immediate reconstruction after resection of mandibular cancer.

A clinical study on the alveolar crest height after secondary alveolar bone graft in the cleft alveolus patients : Preliminary Study

최진영,김명진,김수곤,윤정주,정필훈,Choi, Jin-Young,Kim, Myung-Jin,Kim, Su-Gon,Eune, Jung-Ju,Choung, Pill-Hoon Korean Cleft Lip and Plate Association 2000 대한구순구개열학회지 Vol.3 No.1

목적 : 치조파열 환자에 있어서 이차 치조골 이식술을 시행한 후 그 결과를 알아보고자 방사선학적 분석을 통하여 평가하였다. 본 연구의 목적은 수술전 골결손부 인접치아의 치조정 높이와 수술후 골결손부 인접치아의 치조정 높이를 근심측과 원심측에서 각각 측정함으로써 치조정의 높이가 과연 통계적으로 유의성 있게 증가하는지와 치조골 이식술의 성공률이 치조열의 너비와 상관관계가 있는지의 여부를 알아보는 것이다. 환자 및 방법 : 1991년부터 1999년까지 서울대학교병원 구강악안면외과에서 자가장골 채취 후 분쇄피질망사골 형태 또는 block 피질망상골 형태로 이식한 편측성 치조열을 가진 환자 중 최소한 6개월이 경과한 환자 56명을 연구대상으로 하였고 두 술자에 의해 시술되었다. 수술전 골결손부 인접치아의 치조정의 높이와 치조열의 너비 및 수술후 이식한 골의 높이와 절흔의 양은 치과용 파노라마 방사선 사진을 이용하여 측정하였고, 치조열의 너비는 모델이나 환자의 골결손부 근심치아의 근원심 폭경을 이용하여 환산하였다. 그리고 이식한 골의 높이와 절흔의 양을 1995년 Long이 제시한 방법으로 측정하여 술전의 측정치와 비교하였다. 결과 : 치조열의 너비는 평균 6.9mm(1.9mm-12.1mm) 였다. 근심에서의 치아는 골이식 당시 중절치가 52개(92.9%), 측절치가 4개(7.1%)였고, 49명의 환자에서 완전맹출을, 6명의 환자에서 부분맹출(측절치 2개, 중절치 4개)을 보였다. 원심측에서의 치아는 골이식 당시 측절치가 25개(44.6%), 견치가 29개(51.8%), 소구치가 2개(3.6%)였고, 완전 맹출이 32.1%, 부분 맹출이 57.2%, 미맹출이 10.7%로서 완전히 맹출하기 전에 골이식한 경우가 67.9%였다. 모든 환자에 있어서 bony bridge가 나타났고, 절흔이 인접치 아래로 연장되지 않았으며, 치조골 이식술 후 oronasal fistula를 보인 환자는 한 명도 없었으므로 성공률은 100%였다. 술후 근심측에서의 치조정의 높이는 근심측 치아 치근길이의 79%(평균), 원심측에서의 치조정의 높이는 원심측 치아 치근길이의 87%(평균)로서 통계적으로 유의성 있게 증가하였다. 결론: 이차 치조골 이식술을 시행한 후 치조정의 높이는 골결손부 근심측과 원심측에서 모두 유의성있게 증가하였고, 근심측에서보다 원심측에서 통계학적으로 더 유의성 있게 증가하였다. 치조열의 너비와 절흔의 양, 치조정의 높이 및 치조골이식 성공률과는 유의성 있는 관련성이 없었다.

포스터발표 : 우수 중수도 및 기타 ; 연안어장 준설 퇴적물내 함유된 중금속의 생물침출효율에 대한 에너지원의 영향

송영채 ( Young Chae Song ),염혜경 ( Hae Kyong Yeom ),우정희 ( Jung Hui Woo ),고성정 ( Sung Jung Koh ),김동근 ( Dong Geun Kim ),황응주 ( Eung Ju Hwang ),김명진 ( Myoung Jin Kim ),조규태 ( Kyu Te Jo ) 대한상하수도학회 2004 공동추계 학술발표회 논문집 Vol.2004 No.-

연안어장 준설퇴적물을 이용한 Cochlodinium polykrikoides 제거

선영철,김명진,송영채,고성정,황응주,조규태,Sun, Young-Chul,Kim, Myoung-Jin,Song, Young-Chae,Ko, Seong-Jeong,Hwang, Eung-Ju,Jo, Q-Tae 대한환경공학회 2010 대한환경공학회지 Vol.32 No.1

본 연구에서는 연안어장 준설퇴적물을 이용하여 적조생물인 Cochlodinium polykrikoides을 제거하는 실험을 수행하였다. 최적의 적조제거 조건을 도출하기 위해 준설퇴적물의 투입량 및 입자크기, 열처리, 첨가제 종류 및 함량, 적조 분포 깊이 등의 인자를 조절하였다. 준설퇴적물의 적정 투입량은 6~10 g/L 정도였으며, 이를 살포했을 때 제거율은 60분 후 73~93%이었다. 실제 바다에 투입시 단위면적당(1 $m^2$) 건조한 준설퇴적물 기준으로 6~10 $kg/m^3$를 살포하는 것이 필요하다. 준설퇴적물의 입자크기가 작을수록 적조제거율이 증가했다. 입자크기가 <100 ${\mu}m$일 때 제거율이 93%로 100 ${\mu}m$ <f<200 ${\mu}m$ 입자의 경우(51%)보다 훨씬 높았다. 준설퇴적물의 90% 이상이 100 ${\mu}m$ 이하의 크기를 차지하므로 준설퇴적물을 별다른 처리없이 살포했을 때 상당한 효과를 거두리라고 본다. 준설퇴적물에 첨가제(CaO)를 넣어서 적조제거에 높은 효율(99%)을 얻었다. 첨가제의 적정량은 5~10%이지만 pH 상승을 고려해서 가능한 소량 투입하는 것이 적합하다. 적조 분포 깊이가 깊을수록 제거효율은 증가했다. 깊이가 5 cm 일 때 제거효율이 83%이었고 50 cm 깊이에서는 93%이었다. 바다에서 적조가 발생하는 깊이는 3 m 이내이고, Cochlodinium polykrikoides 대부분이 1 m 이내의 깊이에 존재한다. 이 실험결과를 통해 실제 바다에 준설퇴적물을 적용하면 더 높은 적조 제거효율을 얻을 수 있으리라고 기대한다. 연안어장 준설퇴적물(<100 ${\mu}m$) 10 g/L을 투입하여 적조를 최대 93%까지 제거할 수 있었으며, 이 결과는 황토를 이용한 결과(90~97%)와 비슷했다. 본 연구결과는 연안어장 준설퇴적물 살포법이 기존의 황토살포법을 대체할 수 있을 정도로 처리효율이 뛰어남을 보여주었다. In the present study, experiments have been performed to investigate the possibility of removing Cochlodinium polykrikoides using the dredged sediment from a coastal fishery and then to derive the optimal conditions; the amount and particle size of dredged sediment besprinkled into water, the thermal treatment, the types and amounts of additives, and the depth profile of Cochlodinium polykrikoides. Results showed that the optimal amount of dredged sediment besprinkled into water was 6~10 g/L, and the removal efficiency of Cochlodinium polykrikoides after the reaction time for 60 min was 73~93%. Note that, in the real sea water, it is necessary to besprinkle 6~10 $kg/m^3$ of dry dredged sediment on a unit area (1 $m^2$). With decreasing particle size, Cochlodinium polykrikoides could be more efficiently removed. The removal efficiency was 93% with the dredged sediment smaller than 100 ${\mu}m$, whereas it was 51% with that of 100 ${\mu}m$ <f<200 ${\mu}m$. Since most of dredged sediment (over 90%) was smaller than 100 ${\mu}m$, high efficiency could be obtained by besprinkling only the dredged sediment without pre-treatment. CaO was found to be an effective additive in promoting the removal efficiency (up to 99%). The optimal amount of additive was 5~10%, however, it was necessary to use as small amount of an additive as possible in order to avoid the sharp increase in pH. The removal efficiency increased with increasing depth profile of Cochlodinium polykrikoides. The removal efficiency was 83% at 5 cm depth, whereas it was 93% at 50 cm depth. In the sea water, red tide occurred within 3 m depth, and furthermore most Cochlodinium polykrikoides existed within 1 m depth. It was, therefore, expected that higher removal efficiency of Cochlodinium polykrikoides could be obtained when the dredged sediment was besprinkled into the sea water. The removal efficiency of Cochlodinium polykrikoides was up to 93% when the dredged sediment (<100 ${\mu}m$) was besprinkled into water at the ratio of 10 g/L. This result was comparable to that obtained with loess (90~97%). All the results in the present study indicated that the dredged sediment from a coastal fishery could be successfully used as a substitute of loess for removing the red tide alga.

연안어장 준설 퇴적물내 함유된 중금속의 생물침출효율에 대한 에너지원의 영향

송영채 ( Young Chae Song ),염혜경 ( Hae Kyong Yeom ),우정희 ( Jung Hui Woo ),고성정 ( Sung Jung Koh ),김동근 ( Dong Geun Kim ),황응주 ( Eung Ju Hwang ),김명진 ( Myoung Jin Kim ),조규태 ( Kyu Te Jo ) 한국물환경학회 2004 공동 추계학술발표회 Vol.2004 No.-

구강 편평세포암에서 예후 및 경부임파절 전이와 관련한 증식인자의 발현에 관한 연구

팽준영(Jun-Young Paeng),옥용주(Yong-Ju Ok),윤필영(Pil-Young Yun),명훈(Hoon Myoung),황순정(Soon-Jung Hwang),서병무(Byoung-Moo Seo),최진영(Jin-Young Choi),이종호(Jong-Ho Lee),정필훈(Pill-Hoon Choung),김명진(Myung-Jin Kim) 대한구강악안면외과학회 2006 대한구강악안면외과학회지 Vol.32 No.2

Purpose: The purpose of this study is to evaluate the value of proliferation factors, Ki67 and PCNA, as prognostic markers predicting the survival and neck metastasis in patients with oral cancer. Methods: 101 patients with HNSCCs, were followed retrospectively for a median period of 60 months(from 16 to 82 months). All tumors were resected surgically and examined by conventional light microscopy, immunohistochemistry. The age, sex, tumor location, clinical stage(size), metastasis, proliferative activity index(assessed by proliferating cell nuclear antigen(PCNA) and Ki67 immunoreactivity) were considered as potential prognostic factors and were correlated with patient survival. Results: Ki67 staining results ranged from 5% to 80% of tumor cell nuclei, with a median of 25%. PCNA staining results ranged from 1% to 90% with a median of 50%. With a cut-off point of 25%, patients with lower Ki67 scores showed survival advantages over those with higher Ki67 scores(p=0.0089). With cut-off point of 50%, patients with lower PCNA scores showed survival advantages over those with higher PCNA scores(p=0.0104). Pathologically neck node positive patients(n=27) showed higher PCNA expression(p=0.02) than pathologically negative neck node patients(n=39). Conclusions: The lower expressions of Ki67 and PCNA were associated with favorable prognosis such as higher survival rate and lower neck node metastasis.

악교정수술환자에서 폴리메틸메터크릴레이트(아테콜??) 주입을 통한 융비술

옥용주(Yong-Ju Ok),김명진(Myung-Jin Kim),팽준영(Jun-Young Paeng),명훈(Hoon Myoung),황순정(Soon-Jung Hwang),최진영(Jin-Young Choi),이종호(Jong-Ho Lee),정필훈(Pill-Hoon Choung) 대한구강악안면외과학회 2005 대한구강악안면외과학회지 Vol.31 No.4

Polymethyl-methacrylate(PMMA; Artecoll) microspheres suspended 1 : 3 in a 3.5% collagen solution has been used as an injectable implant for long lasting correction of wrinkles and minor skin defects. The patients with mandibular prognathism have increased necessity for nasal augmentation. Usually these patients usually get an additional rhinoplasty after orthognathic surgery. The purpose of this study is to evaluate the result of PMMA injection for nasal ridge augmentation simultaneously with the orthognathic surgery. PMMAs were injected to the nasal dorsum of 13 patients with mandibular prognathism to augment the nasal ridge at the end of the orthognathic surgery. The cephalometric X-ray and clinical facial photograph were taken at 2, 4 and 6 months after operation. Using S-N line, we calculated the change of soft tisuue on the nasal ridge and also investigated the degree of patients satisfaction at 6 months after operation. Most of the patients were satisfied with their nasal ridge height status from moderate to good degree. The average amount of nasal ridge augmentation was 1.4 ± 0.5 mm immediately after operation, 1.2 ± 0.4 mm at 2 months after operation. The postoperative nasal ridge height seemed to be remained stable after 2 months. Intraoperative PMMA injection is considered to be simple and effective technique which can be used for the minor augmentation of nasal ridge in the orthognathic patients.

구강암 세포주에 대한 CKD-602의 항암 효과

윤필영(Pil-Young Yun),옥용주(Yong-Ju Ok),명훈(Hoon Myoung),이종호(Jong-Ho Lee),김명진(Myung-Jin Kim) 대한구강악안면외과학회 2005 대한구강악안면외과학회지 Vol.31 No.1

Purpose: CKD-602, a newly developed water-soluble campthotecin analogue, is a anticancer agent which act as a DNA topoisomerase I inhibitor. CKD-602 is known as more potent and tolerable agent. The main purposes of this study were to measure the cytotoxic effect of CKD-602 on the oral cancer cell lines and to evaluate the apoptotic aspect of dead cells. Materials and Methods: To determine the cytotoxic effect of CKD-602 on the oral cancer cell lines in comparison with various cell lines, such as lung cancer and colon cancer cell lines, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay was performed. And apoptosis was analyzed using fluorescence-activated cell sorting(FACS) system. Results: CKD-602 decreased the viability of malignant cells in a dose dependent manner and in a time dependent manner. CKD-602 showed excellent cytotoxicity to the oral cancer cell lines. Also, apoptotic portion was increased in a dose dependent manner. Conclusion: These findings indicated that CKD-602 induced apoptotic cell death in the various cell lines including oral cancer cell lines. From the results, it was suggested that CKD-602 would be a potential therapeutic agent for the oral cancer. More successive researches on the anticancer effect of CKD-602 should be performed.