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金龍昊,金奭東,洪殷憙,金秀姬 韓國作物學會 1994 한국작물학회지 Vol.39 No.2
콩 비린내에 관여하는 lipoxygenase가 결핍된 계통들의 가공적성 및 제품개발의 타당성을 알아보고자 두부와 두유제조시 일반콩과의 특성을 비교 검토한 바 그 결과는 다음과 같다. 1. 두부 제조시 lipoxygenase가 일부 결핍된 수원169호는 일반콩인 황금콩에 비해 수율 및 색도는 비슷하였으나 관능검사는 높게 평가되었다. 2. 두부의 일반조성도 공시재료간에는 비슷하였으나 수원169호가 황금콩에 비해 총당 함량은 높게 나타났다. 3. 실험실 규모에서 두유 제조후 관능검사를 실시한 결과 lipoxygenase 결핍계통이 향기 및 맛에서 우수한 것으로 평가되었다. 4. 공장 규모의 두유제조에서도 수원169호가 황금콩에 비해 두유 가공적성이 우수하였다. Lipoxygenase are responsible for offensive grassy and green bean flavors which limit the wide utilization of soy protein products. This study was carried out to investigate the processing characteristics of soybean genotypes lacking lipoxygenase. Soybean curd made from soybeans with and without seed lipoxygenase showed nearly similar yield, while those sensory evaluation was better in lipoxygenase lacking lines than noramal one. Chemical composition of soybean curd also was similar between different genotypes, but total sugar content was higher in lipoxygenase lacking lines. Significant different quality of soymilk was showed by sensory test. The flavor and taste of soymilk were better in soybeans without seed lipoxygenase, although soymilk was made by two different processing methods. Therefore, in soybean breeding pro-gramme for food processing, lipoxygenase-lacking soybean genotypes could be useful economically and nutritionally.
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金龍昊,金奭東,洪殷憙 韓國作物學會 1992 한국작물학회지 Vol.37 No.5
콩에서의 조직배양 가능성을 알아보고자 콩 유묘를 기내에서 배양시킨 뒤, 여러 부위를 채취하여 각기 다른 배지 치상한 결과는 다음과 같다. 1. 줄기정단조직 배양에서는 대부분 shoot가 분화되어 완전한 식물체를 얻을 수 있었다. 2. 자엽절 조직배양에서는 multiple shoots가 나타나 대량증식에 유용했으나 배지에 따른 영향이 컸다. 3. 배축 및 상배축 조직배양에서는 callus만 분화되엇다. 4. 엽조직배양에서는 callus 분화 후 계대배양했을 때 shoot가 분화되었으나 완전한 식물체를 얻기는 힘들었다. To study the capacity of callus and shoot formation on seedling stage in soybean, excised hypocotyl, epicotyl, shoot tip, cotyledonary node and primary leaf were cultured on artificial media (MS and B5 medium) supplemented with several hormones. Regeneration of shoots was fairly successful from shoot tip and cotyledonary node tissues in soybean. These shoots could be rooted in vitro through tissue culture technique and transplanted normally into soil. Hypocotyl and epicotyl tissues formed only callus, of which growth and appearance were different according to the kinds of media and additives. A small number of shoots were formed from primary leaf tissues, but they did not develop further.r.
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金龍昊,金奭東,洪殷憙 韓國作物學會 1994 한국작물학회지 Vol.39 No.3
Lipoxygenase는 콩 비린내 유발의 주원인이 된다. 따라서 lipoxygenase 발현 양상을 알아보고자 콩 생육시기별로 재료를 채취하여 lipoxygenase activity를 측정한 바 그 결과는 다음과 같다. 1. V3 와 V5 시기에 콩 잎에서의 lipoxygenase activity는 유전자형에 상관없이 일정한 경향이 없었다. 2. R6 시기의 /pod wall에서도 유전자형에 따른 lipoxygenase activity의 일정한 경향은 없었으나 종자 lipoxygenase는 lipoxygenase 결핍계통의 activity가 일반품종에 비해 낮았으며 lipoxygenase 결핍계통간에도 isozyme 결핍정도에 따라 activity도 다르게 나타났다. 3. 종자를 종피, 배, 자엽으로 구분하여 각각의 lipoxygenase activity를 측정한 결과 종피에서는 유전자형에 따른 경향을 찾을 수 없었으나 배와 자엽에서는 lipoxygenase isozyme 결핍 정도에 따라 activity도 다르게 나타났다. Lipoxygenase is involved in the formation of certain undesirable flavors of soybean products. Three isozymes(L-1, L-2 and L-3) of lipoxygenase have been identified in soybean seeds, and the three types of mutants lacking L-1, L-2 and L-3, respectively, were detected in the 1980's. In this paper, lipoxygenase activity was measured to investigate the response of lipoxygenase in organs and tissues during soybean development. There was no tendency according to genotypes between lipoxygenase lacking mutants and normal soybeans in lipoxygenase activity of leaf at V3 and V5 stage. Likewise, pod wall lipoxygenase was no difference among genotypes tested at R6 stage. Seed coat lipoxygenase activity was similar among the lipoxygenase lacking mutants, while normal soybean was lower as compared with that of the lipoxygenase lacking mutants. Embryo and cotyledon lipoxygenase activity in the lipoxygenase lacking mutants was much lower than that of normal soybean, also there was large difference among lipoxygenase lacking mutants. Thus, the lipoxygenase null mutant showed very weak value although the lacking L-3 mutant had a large effect on developing embryo lipoxygenase activity. It was suggested that soybean lipoxygenase isozymes expressed in embryo may be different from those expressed in the pod wall and leaf tissues.
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金龍昊,金奭東,洪段憙,安完植 韓國作物學會 1996 Korean journal of crop science Vol.41 No.S
Soyfoods have potential roles in the prevention and treatment of chronic diseases, most notably cancer, osteoporosis, and heart disease. There is evidence that carcinogenesis are supressed by isolated soybean derived products in vivo such as a protease inhibitor, phytic acid, saponins and isoflavones. It is believed that supplementation of human diets with soybean products markedly reduces human cancer mortality rates. Especially, recent papers recognize the potential benefit of soybean isoflavone components for reducing the risk of various cancers. Isoflavones exhibit a multitude of medicinal effects that influence cell growth and regulation, which may have potential value in the prevention and treatment of cancer. In addition to potential biological effects, soybean isoflavones have the important physiological functions such as the induction of Bradyrizobium japonicum nod genes and the responses of soybean tissues to infection by Phytophthora megasperma as well as biochemical activities such as antifungal and antibacterial actions. Genistin, daidzin, glycitin and their aglycone (genistein, daidzein, glycitein) are the principal isoflavones found in soybean. Malonyl and acetyl forms have also been detected but they are thermally unstable and are usually transformed during the processing in glucoside form. Most soy products, with the exception of soy sauce, alcohol-extracted soy protein concentrate, and soy protein isolate, have total isoflavone concentrations similar to those in the whole soybean. Soybean-containing diets inhibit mammary tumorigenesis in animal models of breast cancer, therefore, it is possible that dietary isoflavones are an important factor accounting for the lower incidence and mortality from breast cancer. Of the total soybean seed isoflavones, 80~~90~% were located in cotyledons, with the remainder in the hypocotyls. The hypocotyls had a higher concentrations of isoflavones on a weight basis compared with cotyledons. Isoflavone contents were influenced by genetics, crop years, and growth locations. The effect of crop year had a greater impact on the isoflavone contents than that of location. The climate condition might be the attribution factor to variation in isoflavone contents. Also, while the isoflavone content of cotyledons exhibited large variations in response to high temperature during seed development, hypocotyls showed high concentration in isoflavone content. So, it is concluded that one of the factors affecting isoflavone content in soybean seeds is temperature during seed development. High temperature, especially in maturity stage, causes lower isoflavone content in soybean seed. It is also suggested that there may exist a different mechanism to maintain isoflavone contents between cotyledon and seed hypocotyls. In a conclusion, soy foods may be able to have a significant beneficial impact on public health.
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김룡호,최병현,김영직,오현주,김병주,Xiang Yun Yin,민병현 한국조직공학과 재생의학회 2018 조직공학과 재생의학 Vol.15 No.3
It is very useful to evaluate the content and 3D distribution of extracellular matrix non-destructively in tissue engineering. This study evaluated the feasibility of using micro-computed tomography (lCT) with Hexabrix to measure quantitatively sulfated glycosaminoglycans (GAGs) of engineered cartilage. Rabbit chondrocytes at passage 2 were used to produce artificial cartilages in polyglycolic acid scaffolds in vitro. Engineered cartilages were incubated with Hexabrix 320 for 20 min and analyzed via lCT scanning. The number of voxels in the 2D and 3D scanning images were counted to estimate the amount of sulfated GAGs. The optimal threshold value for quantification was determined by regression analysis. The 2D lCT images of an engineered cartilage showed positive correlation with the histological image of Safranin-O staining. Quantitative data obtained with the 3D lCT images of 14 engineered cartilages showed strong correlation with sulfated GAGs contents obtained by biochemical analysis (R2 = 0.883, p\0.001). Repeated exposure of engineered cartilages to Hexabrix 320 and lCT scanning did not significantly affect cell viability, total DNA content, or the total content of sulfated GAGs. We conclude that lCT imaging using Hexabrix 320 provides high spatial resolution and sensitivity to assess the content and 3D distribution of sulfated GAGs in engineered cartilages. It is expected to be a valuable tool to evaluate the quality of engineered cartilage for commercial development in the future.
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Pineapple에 있어서 生長調節物質 處理에 依한 開花促進과 收穫期調整에 관한 硏究
吳現道,白子勳,金龍湖 제주대학교 1983 논문집 Vol.15 No.-
本 硏究는 pineapple 植物에 대하여 植物生長調節物質 處理에 依한 開化誘導 收穫期 調整에 있어서 效果的인 方法을 究明하기 爲하여 遂行되었다. 그 結果를 要約하면 다음과 같다. 抽苔期는 acethylene gas (carbide) 施用區에서 가장 反應이 빨리 나타났으며 ??-naphthalene acetic acid 10ppm 1回, 2回 處理區 및 a-naphthalene acetic acid 50ppm 1回 處理區에서 그 다음 順位였고, ??-nphthalene acetic acid 50ppm 2回 및 3回 處理區에서는 反應이 가장 늦게 나타났다. 全體 開花 效果는 ??-naphthalene acetic acid 50ppm 3回 處理區에서 83.3%로 가장 낮았고, ethylene gas(ethrel)200ppm, 1,000ppm 施用區에서 各各 94.3%를 나타내었으며 그 外의 處理區에서는 100% 의 開花率을 보였다. 果實의 收量에 있어서 抽苔가 빠른 acethylene gas 및 ??-naphthalene acetic acid 10ppm 1回 處理區에서 平均 果實收量(1.45kg, 1.42kg)이 增收하였고, 開花效果가 不進하였던 收量指數가 61.3으로 가장 낮았다. 冠芽의 크기는 開花期가 빨랐던 處理區에서 果實의 收量과 類似한 傾向으로 增加함을 보였다. This study was conducted to investigate the effect of the growth substances(acethylene gas, ??-naphthalene acetic acid, and ethylene) on the control of flowering induction and harvesting in pineapple plant. The results obtained are as follows; The earliest earing was gained in the plot treated with acethylene gas(carbide). The considerable effect was producde in the plots treated with ??-naphthalene acetic acid 10ppm once or twice and with ??-naphthalene acetic acid 50ppm one time. But in the plot treated with ??-naphthalene acetic acid 50ppm three times, the reaction was not so effective. The effect of flowering induction was lowest(83.3%) in the plot treated with ??-naphthalene acetic acid 50ppm three times, and in the plots treated with ethylene gas (ethrel) 200ppm and 1,000ppm, the rate of flowering was 94.4% respectively. Except those plots, the rate of flowerining induction were 100% The yield of fruit increased in the polt treated with acethylene gas and with ??-naphthalene acetic acid 10ppm one time (1.45kg, 1.42kg respectively). And in the plot treated with ??-naphthalene acetic acid 50pm three times, the yield index was lowest (61.3). The size of crown tended to be enlarged in propotion to the rate of fruit yield in the plot in which the effect of flowering induction was effective.
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