수용액상에서 뽕잎의 염소 제거 효과

김동청,채희정,인만진 한국잠사학회 2000 한국잠사곤충학회지 Vol.42 No.2

In this study, a comparative removal of chlorine from aqueous solutions of mulberry leaf powder(MLP) and activated carbon(AC) was investigated. The chlorine removal capacities of MLP and AC were shown as a function of contact time, pH and initial chlorine concentration. Optimum contact time and removal pH value of MLP were determined as 2 hr and pH 10, respectively. Chlorine removal increased with increasing initial chlorine concentration up to 1.3 g/L. Both Langmuir and Freundlich adsorption models were suitable for describing the short-term removal of chlorine by MLP and AC According to Freundlich adsorption isotherms, the maximum removal capacity of MLP(0.264 mgCl,/mg) was nearly two times greater than that of AC(0.156 mg Cl_2/mg). These results suggested that MLP might potentially be used as an alternative to traditional water treatment materials for removal of residual chlorine in drinking water or process wastewater.

인삼의 polyacetylene계 성분이 Ras 단백질 변형 효소의 활성에 미치는 영향과 인삼의 석유에텔 추출물이 세포주기에 미치는 영향 ( Effects of Polyacetylenes in Ginseng on Activity of Enzymes Related to Post-translational Modification of Ras Protein and Effects of Petroleum Ether Extract of Ginseng on Progression of Cell Cycle )

김동청,이지영,인만진,채희정,황윤경,황우익 고려인삼학회 2001 Journal of Ginseng Research Vol.25 No.4

대두 ( Glycine max ) 하배축에서 ornithine decarboxylase 의 정제 및 특성에 관한 연구

김동청,김병현,조영동 생화학분자생물학회 1992 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

대두 ( Glycine max ) 하배축에서 정제한 Arginine decarboxylase 의 활성 억제에 관한 연구

김동청,박영철,최연식,조영동 생화학분자생물학회 1991 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

홍삼 추출물의 제조에서 단백질 분해효소의 활용

김동청,이태정,인만진 한국응용생명화학회 2019 Journal of Applied Biological Chemistry (J. Appl. Vol.62 No.4

In this study, proteolytic enzymatic treatment conditions for Korean red ginseng were examined to increase the extraction yield. Commercially available proteases were screened to obtain high protein and carbohydrate yield. The optimal dosage and reaction time for Alcalase, the chosen protease, were found to be 2.0% (w/w) and 1.5 h, respectively. Treatment with optimal conditions of Alcalase increased solid yield, total phenolic content and gensenosides content by 57.6, 81.8, and 33.8%, respectively, over levels in non-treated Korean red ginseng. Antioxidative activities evaluated by free radical scavenging activity, cation radical scavenging activity and reducing power were exactly similar between Alcalase-treated and non-treated extracts. 본 연구에서는 홍삼 추출물 제조과정에서 추출 수율을 향상시킬 수 있는 효소를 선별하고 최적의 반응조건을 조사하였다. 상업용 단백질 분해효소 중 Alcalase가 단백질과 탄수화물 수율향상에 효과적이었으며, 효소 사용량은 홍삼 중량의 2%, 반응시간은 1.5시간이 적당하였다. 최적의 반응조건으로 홍삼을Alcalase로 처리한 결과 대조구보다 고형분 수율은 45.1%에서 71.1%로 50% 이상, 총페놀 함량은 0.44%에서 0.80%로 80% 이상 증가하였으며, 항산화 활성은 대조군과 매우 유사하였다. 또한 진세노사이드 함량은 대조구의 1.48 mg/g에서 1.98 mg/g으로 30% 이상 증가하였다.

두충나무(Eucommia ulmoides Oliver) 잎과 껍질의 에탄올 추출물의 항산화 활성

김동청 한국응용생명화학회 2020 Journal of Applied Biological Chemistry (J. Appl. Vol.63 No.3

Antioxidative activities of 50% ethanolic extracts from Du-zhong (Eucommia ulmoides Oliver) leaf and bark were investigated. Yields of the leaf and bark extract were 8.1±0.31 and 17.4±0.89%, respectively. Polyphenol contents of the leaf and bark extract were 64.1±3.35 and 42.4±2.38 μg gallic acid equivalents/ mg, respectively. Flavonoid contents of the leaf and bark extract were 24.0±3.15 and 36.7±3.18 μg quercetin equivalents/mg, respectively. As concentration of the leaf and bark extract increased, their antioxidative activities proportionally increased. EC50 values of the leaf and bark extract for cation radical scavenging were 560.6±17.65 and 1,357.4±8.45 μg/mL, respectively. EC50 values of the leaf and bark extract for free radical scavenging were 574.2±14.70 and 2,103.1±108.59 μg/mL, respectively. EC50 values of the leaf and bark extract for ferric reducing antioxidant power were 319.9±13.42 and 705.9±26.08 μg/mL, respectively. EC50 values of the leaf and bark extract for nitrite scavenging were 2,329.2±35.11 and 5,467.6±243.92 μg/mL, respectively. In the presence of 74.8 μg/mL of the leaf extract and 177.2 μg/mL of the bark extract, linoleic acid peroxidation was inhibited by 70.0 and 79.1%, respectively. The Du-zhong leaf extract possessed higher antioxidative activities than its bark extract. 두충나무(Du-zhong, Eucommia ulmoides Oliver)의 잎과 껍질로부터 얻어진 50% 에탄올 추출물의 항산화 활성을 확인하였다. 두충나무 잎과 껍질 추출물의 수율은 각각 8.1±0.31과 17.4±0.89%, 총 폴리페놀 함량은 각각 64.1±3.35와 42.4±2.38 μg GAE/mg, 총 플라보노이드 함량은 각각 24.0±3.15와 36.7±3.18 μg QE/ mg으로 나타났다. 두충나무 잎과 껍질 추출물은 농도에 비례하여 양이온라디칼, 유리라디칼과 아질산염을 소거하였고, 환원력을 증가시켰으며 지질과산화를 억제하였다. 두충나무 잎과 껍질추출물의 ABTS 양이온라디칼 소거에 대한 EC50값은 각각560.6±17.65와 1,357.4±8.45 μg/mL이었고, DPPH 유리라디칼 소거에 대한 EC50값은 각각 574.2±14.70과 2,103.1±108.59 μg/ mL로 나타났다. 또한 두충나무 잎과 껍질 추출물의 환원력에대한 EC50값은 각각 319.9±13.42와 705.9±26.08 μg/mL였고, 아질산염 소거에 대한 EC50값은 각각 2,329.2±35.11과 5,467.6±243.92 μg/mL로 나타났다. 두충나무 잎 추출물은 74.8 μg/mL의 농도에서, 껍질 추출물은 177.2 μg/mL의 농도에서 linoleic acid의 과산화를 각각 70.0 및 79.1% 억제하였다. 두충나무 잎 추출물은껍질 추출물에 비해 높은 항산화 활성을 보유하고 있었다.

고려인삼의 항암 활성의 최근 연구 동향

김동청,Kim, Dong-Chung 고려인삼학회 2010 고려인삼연구와 산업 Vol.4 No.2

A Protocol for Batch Fermentation for Production of Soluble Alpha Interferon by Recombinant Escherichia coli

김동청,정진 한국응용생명화학회 2002 Journal of Applied Biological Chemistry (J. Appl. Vol.45 No.4

Improvement of alpha interferon (IFNα) production was attempted using recombinant E. coli by optimizing fermentation conditions. A protocol that ensures an increased productivity of IFNα mostly in active, soluble form without affecting the growth activity of cells was devised. Although the cells grew well in a neutral medium, a slight decrease in medium pH,i.e. to pH 6.7, resulted in a significant increase in the production of soluble IFNα. The growth activity and total IFNα productivity were not altered by a change in fermentation temperature in the range of 30-37℃. Ratio of soluble form to inclusion body increased with decreasing temperature, with over 90% of total interferon being of soluble form when cultivated at 30℃. An increase in the nitrogen concentration of Terrific Broth-based medium supplemented with L-leucine gave remarkably improved results. A fermentation protocol derived from these observations enabled us to enhance the productivity level of IFNα over 200 x 10 exp (8) IU/-broth by 14h fermentation.

효소로 당화시킨 주박 분해물을 첨가한 요구르트의 발효 특성

김동청,원선임,인만진 한국응용생명화학회 2018 Journal of Applied Biological Chemistry (J. Appl. Vol.61 No.4

This study was carried out to determine the effect of enzymatically saccharified Korean rice wine lees powder (eKRWLP) on the quality characteristics and storage stability of curd yogurt. Yogurt with different contents [0.5-2.0% (w/w)] of eKRWLP was incubated with commercially available mixed lactic acid bacteria (Lactobacillus acidophilus, Streptococcus thermophilus, Bifidobacterium longum) at 40 oC for 18 h. The production of acid measured at pH and titratable acidity of yogurts increased with increasing eKRWLP content. After 12 h fermentation, titratable acidity of eKRWLP yogurt was 0.77-0.90% and was higher than that (0.72%) of yogurt made without eKRWLP. The viable cell counts of lactic acid bacteria in eKRWLP yogurts were increased in proportion to the addition of eKRWLP, and increased up to 8.01- 8.13 log CFU/g after 12 h incubation. The repressive effect of whey separation in eKRWLP yogurt curd significantly decreased than that in Korean rice wine lees powder (KRWLP) yogurt. With sensory evaluation, yogurt with 0.5% eKRWLP obtained the highest scores among all eKRWLP yogurts. When eKRWLP yogurts and the control preparations fermented for 12 h were incubated at 4 oC, their pHs and titratable acidities were slightly changed and the number of viable lactic acid bacteria were well maintained above 107 CFU/g for 16 days. 본 연구에서는 효소 처리한 주박 당화물 분말을 발효식품에 이용하고자 상업용 Lactobacillus acidophilus, Streptococcus thermophilus, Bifidobacterium longum 혼합 균주를 이용하여탈지분유에 주박 당화물을 첨가하여 요구르트를 제조하고 주박당화물 분말의 첨가량에 따른 요구르트의 발효특성과 저장성을조사하였다. 탈지분유에 주박 당화물을 0.5-2.0% 첨가한 결과적정산도는 주박 당화물의 사용량에 비례하여 증가하였으며, 12 시간 후 대조군 0.72%에서 주박 당화물 첨가 요구르트는 0.77- 0.90%로 증가하였다. pH의 변화도 적정산도의 변화와 일치하는경향이었다. 젖산균 생균수는 주박 당화물의 첨가량에 비례하여증가하였으며, 12시간 배양 후 대조군은 7.83 log CFU/g에서주박 당화물 첨가군은 8.01-8.13 log CFU/g까지 증가하였다. 또한 주박 당화물은 주박과는 상이하게 커드의 안정성에 대한 효과가 매우 미미하였다. 관능평가 결과. 주박 당화물의 첨가 수준은 0.5%가 적절하였다. 주박 당화물을 첨가한 요구르트를4 oC에서 16일간 저장하는 동안 pH와 적정산도의 변화는 미미하였고, 젖산균 수는 약간 감소하였으나 모든 조건에서 107 CFU/g 이상으로 유지되었다.

홍차박 추출물의 in vitro 항산화 활성

김동청 한국응용생명화학회 2019 Journal of Applied Biological Chemistry (J. Appl. Vol.62 No.3

홍차를 우려내고 남은 부산물인 홍차박을 30% 에탄올로 추출하여 수율, 폴리페놀 함량 및 항산화 활성을 확인하였다. 홍차박의 추출 수율은 22.4±1.18%이었고, 폴리페놀은 추출물에23.2±1.02 μg GAE/mg 들어있었다. 홍차박 추출물은 양이온라디칼, 유리라디칼 및 아질산염을 농도에 비례하여 소거하였고, 환원력과 지질과산화 억제활성도 농도의존적으로 증가하였다. 홍차박 추출물의 ABTS 양이온라디칼, DPPH 유리라디칼 및 아질산염 소거에 대한 IC50값은 각각 141.8, 108.1 및 397.2 μg/m이었고, 환원력에 대한 IC50값은 97.8 μg/mL로 나타났다. 또한홍차박 추출물은 linoleic acid의 과산화를 효과적으로 억제하였다. 결론적으로 홍차박 추출물은 우수한 항산화 활성을 보유하고 있어 생리활성 소재로의 활용 가능성을 보여주었다. A black tea (Camellia sinensis L.) residue extract (BTRE) was prepared by 30% ethanol extraction to evaluate its in vitro antioxidant activity. The yield and polyphenol content of BTRE were 22.4±1.18% and 23.2±1.02 μg gallic acid equivalents/ mg-extract, respectively. Antioxidant activity of BTRE proportionally increased as BTRE concentration increased. IC50 values of BTRE for cation radical, free radical and nitrite scavenging were 141.8, 108.1, and 397.2 μg/mL, respectively. Also IC50 value of BTRE for ferric reducing anti-oxidant power was 97.8 μg/mL. BTRE effectively inhibited linoleic acid peroxidation. These results imply that BTRE possessed potent antioxidant activity, thus being utilized as a physiologically active material.