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Campylobacter fetus Subspecies jejuni에 관한 고찰
김대근 ( Dae Keum Kim ),박종윤 ( Jong Yoon Park ),이상열 ( Sang Youl Lee ),박종암 ( Chong Ahm Park ),김경숙 ( Kyung Suk Kim ),윤현정 ( Hrun Jung Yun ) 대한임상검사과학회 1982 대한임상검사과학회지(KJCLS) Vol.14 No.1
Campylobacter fetus subsp. jejuni was lsolated from clinical material (stool, rectal swab) at HanYang University Hospital during the period of November 1981 to June 1982. Result obtained are as follow 1. The number of organisms identied as C. fetus Subsp. jejuni was 18(5.4%) from 332 specimens. They are all from the diarreal patients under the age of 2 years old. 2. Susceptibtlity to antibiotics of these strains revealed Amikacin 100%, Gentamicin 100%, Chloramphenicol 95%, Tobramycin 95%, Minocyclin 95% and Kanamycin 78%. 3. C. fetus subsp. jejuni was not isolated from the adult specimens.
김금용(Keum Yong Kim),김진형(Jin Hyung Kim),김대근(Dae Keun Kim),류홍덕(Hong Duck Ryu),이상일(Sang Ill Lee) 大韓環境工學會 2008 대한환경공학회지 Vol.30 No.2
본 연구는 Fill-Contact-Settle-Decant-Idle의 공정으로 구성된 활성슬러지 공정인 SBR(Sequencing bach reactor)공정에서 수리학적 체류시간을 24시간으로 하여 운전하였으며, 세포외 폴리머의 생성량, 흡착량, 제거율 등과 SRT와의 상관관계에 대한 특성을 조사하였다. 본 실험의 목적은 활성슬러지 시스템에서 다양한 SRT를 통한 생물흡착 특성을 결정하기 위한 것이다. 연구 결과 SRT(Sludge retention time)가 증가함에 따라 단위 미생물당 유기물 흡착율 및 제거율이 감소하는 것으로 나타났으며 단위 미생물당 생성되는 세포외 폴리머 양도 감소하는 것으로 나타났다. 생물흡착효율은 SRT가 증가할수록 높았고 SRT 30일에서 그 값은 53.2%였다. 하지만 단위 미생물당 생물흡착량은 SRT 2일에서(48.6 mg COD/gVSS) 가장 높게 관찰되었다. SRT에 따른 EPS량은 TSS, TCODCr 그리고 TKN에 의해 정량적으로 분석하였다. 상기의 결과로 인하여 슬러지의 플록 형성을 방해하여 슬러지의 침강성을 저해하며, COD 제거효율을 감소시키는 결과를 초래하는 것으로 사료된다. 따라서 미생물을 이용한 생물학적 공정 운영시 EPS의 특성을 고려하여 SRT, MLSS 유지, 유기물 부하량 등의 설계 인자를 적정히 선정하여 운영하는 것이 필요할 것으로 판단된다. The objective of this study was to investigate biosorption of organic matter on EPS(Extracellular Polymeric Substances) at different SRT(Sludge Retention Time) in a SBR(Sequencing Batch Reactor) process, which was operated with the following operation steps: Fill-React-Settle-Decant-Idle. The hydraulic retention time was set to be 24 hours. The results obtained from this study showed that the organic removal efficiency per unit microbial biomass decreased with increasing SRT, and the corresponding EPS amount also did. The percent removal of organic by biosorption increased with SRT, and it reached to 53.2% at SRT of 30 days. However, the highest biosorption per microbial biomass(48.6 mgCOD/gVSS) was found at SRT of 2 days. The EPS analysis was performed by measuring TSS, TCODCr, and TKN. The EPS production per unit microbial biomass was observed to be high at a low SRT. Due to the above result, the floc formation was hindered and therefore poor settlement of sludge resulted in decreasing the COD removal efficiency. It was therefore concluded that the consideration of the system design should include the characteristic of EPS as well as other factors such as SRT, MLSS, and organic loading.
Struvite 결정화에 미치는 영향; Seed 물질, Seed 입자크기, G·t<sub>d</sub> Value의 영향
김진형(Jin Hyoung Kim),김금용(Keum Yong Kim),박형순(Dae Keun Kim),김대근(Hyoung Soon Park),이상철(Sang Cheol Lee),이상일(Sang Ill Lee) 大韓環境工學會 2008 대한환경공학회지 Vol.30 No.2
본 연구에서는 seed 물질을 첨가함으로써 struvite 결정핵의 생성 및 성장단계를 단축시키고자 하였다. 본 연구에서는 seed물질의 성상(모래, 안트라사이트, struvite)별, 입자의 크기(44~63 μm, 63~88 μm, 88~114 μm)별, 교반조건(G.td)에 따라 결정핵의 생성속도론에 미치는 영향을 관찰하였다. 모래와 안트라사이트는 seeding 하지 않은 경우에 비하여 암모니아성 질소제거효율이 각각 9%, 11%로 향상되었으며, struvite로 seed한 경우는 암모니아성 질소제거효율은 20% 이상 향상되었다. Seeding에 따른 struvite 결정화 효율은 seed 입자의 비표면적과 밀접한 상관관계가 있다. Seed 물질입자의 비표면적이 클수록 struvite 핵생성 및 성장을 향상시켰다. 또한 struvite 결정화 반응시 동질의 seed 물질(struvite seeds)을 사용하여 2차 핵생성을 유도하면 struivte 결정의 핵생성 및 성장을 위한 G·td값을 단축시킬 수 있는 것으로 나타났다. 본 연구에서 고려한 입자크기에 대해서는 seed 입자크기에 대한 영향은 확인되지 않았다. Struvite 결정핵 생성속도론에서 G·td값은 매우 중요한 인자로써 작용하였다. This study focused on shorten the period of the struvite crystal birth and development by adding seed materials. For this purpose, three different seed materials were selected: sand, anthracite and struvite. The experiments has been conducted to evaluate the effect of the particle size of the selected seed material on the struvite crystallization, and to study the mixing effect which can be expressed by the value of G·td(the multiple of mean velocity gradient(G) and mixing time(td)). It was observed in this study that the removal efficiency of ammonia nitrogen increased by 9%, 11%, and 20% for sand, anthracite, and struvite added as the seed material, respectivley. This indicated that the struvite crystallization efficiency had a close correlation with the specific surface area of the seed particle. It was found that when struvite was selected as the seed material, the struvite crystallization proceeded at lower G·td value as compared with other seed materials. This observation implied that the secondary crystal birth would be dominated in this reaction. It was concluded in this study that the particle size was not significant factor on the struvite crystallization, while the G·td value was a considerably important factor in terms of the theory of the struvite crystal birth.
PEG(Polyethylene glycol) antiglobulin test를 이용한 항체선별검사의 평가
이종숙 ( Jong Sook Lee ),김덕언 ( Duck Ean Kim ),김대근 ( Dae Keum Kim ) 대한임상검사과학회 1998 대한임상검사과학회지(KJCLS) Vol.30 No.2
The polyehylene glycol antiglobulin test for detection of red cell antibodies was compared with a proven, highly sensitive test system using bovine serum albumin(BSA). All specimens recived in the blood bank over 1-year period for transfusion requests or preoperation patients for roution antibody screening test. The sera of 73 of 6734 patients had detected antibodies. Nine c1inically significant antibodies (1anti-Jka, 2-E, 1-E,c, 3-c, 2-Lea) and four antibodies(l -anti-Jka,Fyb’ 1-C, 1-Fyb, 1-warm auto) demonstrated inconc1usive PEG method. Eight patients had multiple antibodies. We used polyspecific antihuman globulin serum but 1.23% nonspecific reactions by PEG method.
Cisplatin의 신장독성에 대한 영지추출물 복합제제의 보호효과
김대근,김근중,주성민,김용익,최호승,금경수,김원신,고익괴,전병훈 대한동의생리학회,대한동의병리학회 2003 동의생리병리학회지 Vol.17 No.2
In this paper, the effect of Ganopoly(extracts of Ganoderma lucidum) and Ganopoly/C^+(70% Ganopoly + 30% chitosan) on cisplastin-induced nephrotoxicity was investigated in Sprague-Dawley rats. A single dose of dispiastin(5 ㎎/kg) was administered intraperitoneally after pretreatment of saline, Ganopoly and Ganopoly/C^+ for 7 days. The nephrotoxicity and renal function were manifestated by the changes of body weight, blood pressure, biochemical changes and solute in urine and plasma. After the treatment of CDDP(cis-dichlorodiamineplatinum), a significant elevation of kidney weight, serum urea, cretinine, urine volume for 24 hours, urine magnesium, and a severe or significant decrease in body weight, blood pressure, creatinine clearance, urine osmolarity, serum albumin, etc. The nephrotoxicity was further confirmed by a significant decrease in glutathione S-transferase(GSH) in urine and kidney homogenate, GSH, glutathione peroxidase(GSH-Px) and catalase in kidney tissue. And also the lipid peroxidation was significantly increased in kidney homogenate. These signs of nephrotoxicity was ameliorated by the pretreatment and consecutive administration of Ganopoly and Ganopoly/C^+ for 14 days after the i.p. injection of CDDP on 7th day after pretreatment of Ganopoly and Ganopoly/C^+. The amelioration of nephrotoxicity was evidenced by significant reduction in serum urea and creatinine concentration, and improvement of other index of renal function. And The activity of antioxidant enzymes were partially recovered in kidney tissue of rats treated by CDDP and the administration of Ganopoly and Ganopoly/C^+. These results indicate the cispastin induced nephrotoxicity is due to an impairment of tubular reabsorption systems enhanced by necrosis of proximal tubule, and the Ganopoly and Ganopoly/C^+ has a partial protective effect on nephrotoxicity induced by CDDP. The polysacchride of Ganoderma lucidum may improve the therapeutic index of nephrotoxicity induced by CDDP. However, it is needed to elucidate the mechanism for confirming the therapeutic effect.
애기마름(Trapa pseudoincisa S. et. Z.)으로부터 sterol 화합물의 분리 및 구조동정
양혜정,송명종,노영덕,김대근,백남인 경희대학교식량자원개발연구소 2007 硏究論文集 Vol.26 No.1
애기마름을 80% MeOH로 추출하고, 얻어진 추출물을 EtOAc, n-BuOH 및 H_(2)O로 용매 분획 하였다. EtOAc 분획으로부터 silica gel과 octadecyl silica gel(ODS) column chromatography를 반복 실시하여 2종의 화합물을 분리하였다. 각 화합물의 화학 구조는 NMR, MS 및 IR 등의 스펙트럼을 데이터를 해석하여, stigmasterol과 stigmasterol 3-O-β-D-glucopyranosyl로 구조 동정하였다. 이화합물은 애기마름에서는 처음 분리되었다. Trapa pseudoincisa was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and H_(2)O. Two compounds were isolated from the EtOAc fraction through repeated silica gel and ODS column chromatographies. From the result of spectral data including NMR, MS, IR and GC/MS the chemical structures of the compounds were determined as stigmasterol (compound Ⅰ), and stigmasterol 3-O-β-D-glucopyranosyl (compound Ⅱ). They were isolated from Trapa pseudoincisa for the first time.