여드름 환자의 혈중 Testosterone 및 Dehydroepiandrosterone - sulfate 농도에 관한 연구

김낙인(Nack In Kim),오희수(Hee Soo Oh),이상수(Sang Soo Lee),이무형(Moo Hyung Lee),허충림(Choong Rim Haw) 대한피부과학회 1989 대한피부과학회지 Vol.27 No.1

This study was performed to detect the hormonal abnormalities and to investigate the relationship between the levels of plasma testosterone and DHEA-S in 21 female patients with acne vulgaris using radioimmunoassay. The results were as follows : 1. The levels of plasma testosterone in patients group and control group(n=9) were 352+11.8 ng/dl, 223+ 9.7 ng/dl, respectively, and significantly elevated in patient group(p < 0.01), 2. The levels of plasma DHEA-S in patient group and control group were 286.6+ 126.8 pg/dl, 238.6+60.0 pg/dl, respectively, and did not show statistical significance. 3. The levels of plasma testosterones in patient subgroups classified by modified Pillsbury method were as follows minor group 32.6+5.3 ng/dl, mild group 33.4+15,5 ng/dl, moderate group 39.6+12.6 ng/dl. The testosterone levels in all the subgroups increased significantly than those in control group(352-t11.8 ng/dl) (p<0.05, p<0.05 p < 0.01) 4. The levels of plasrna DHEA-S in patient subgroups were follows minor group 242.5+412 pg/dl, mild group 263.9+166.1 pg/dl, moderate group 353.4+-1273 pg/dl. The DE3EA-S levels in moderate group increased significantly than those in cotrol (238,6+60,0 pg/dl) and minor group(p<0.05). 5. There was no correlation between the plasma testosterone levels and the plasma DHEA-S levels in the acne patients.

건선에서의 보습제

김낙인 ( Nack In Kim ) 한국피부장벽학회 2007 한국피부장벽학회지 Vol.9 No.1

Psoriasis is an inflammatory skin disease that commonly causes itching and scaling. Because of its chronic and recurring nature, psoriasis can affect a person`s quality of life. In this lecture, we will see the effects of psoriasis on skin, especially on skin dryness. The decision to employ a particular treatment is based on the type of psoriasis, its location, extent, severity, and the age and gender of the patients. There can be variations between individuals in the effectiveness of specific psoriasis treatments such as topical, systemic treatment and phototherapy. Among the topical treatments for psoriasis, the most important and basic consideration is use of a skin moisturizer. These products produce an occlusive film that limits evaporation of water from the skin and allows the stratum corneum to rehydrate itself. Hydration of the stratum corneum can also lead to enhanced delivery of other medications, such as corticosteroids. In psoriatic skin lesions, it is well established that the transepidermal water loss (TEWL) values increase and decrease when a moisturizer is applied, so these values have also been used to monitor the efficacy of active treatments for psoriasis. Because dryness in psoriasis is the principal cause for increased severity, using the proper moisturizer is necessary and beneficial for patients suffering with psoriasis.

인체 사이토카인과 혼합 림프구 유도 사이토카인이 인조피부 표피의 증식과 분화에 미치는 영향

하태영,김낙인 ( Tai Young Har,Nack In Kim ) 대한피부과학회 1997 대한피부과학회지 Vol.35 No.4

Background: A living skin equivalent(LSE) is useful as a skin replacement and as a model system for basic studies such as skin physiology and pathology. This system attempts to reproduce in vitro the cell-cell and cell-matrix interactions responsible for cell differentiation. Recently, there have been many studies that cytokines(IL-1, IL-6, IL-8, TGF-b, TNF-a) play an important role in proliferating skin disease, especially psoriasis. Hesides, many cytokines can influence the proliferation and differentiation of normal human keratinocytes. Objective and Methods . In the present study, the author investigated the proliferation and differentiation of cytokines in LSE as compared with control skin using histopathological and immunohistochemical staining methods. The author also investigated the proper concentration of cytokines on the proliferation of cultured human keratinocytes. Results '. Morphological analysis of LSE showed reorganization of epidermal layers with the appearance of a distinct basal laer and of a hyperkeratotic horny layer. We demonstrated that the fine granules resembling keratchyaline granules were observed in the control and cytokine treated groups(IL-la, IL-2, IL-6, TNF-a). We observed significant epidermal proliferation in the PBMC (25%), IL 6(10ng), TGF-g(20ng) treated groups than in the control group(p<0.01). Immunohistochemical analysis demonstrated that the terminal differentiation marker involucrin was expressed at the level of the prickle cell layer in the cytokine treated groups. Also the cellular proliferation marker PCNA was expressed at the level of the basal layer in the PBMC(25%), IL-2(100ng) and IL-6(10ng) treated groups. Conclusion : The results indicate that cytokine IL-6 and TGF-b can induce a proliferation and differentiation of the epidermis in this in vitro model. (Kor J Dermatol 1997;35(4): 674-683)

건선 환자에서 involucrin , loricrin , filaggrin , bcl-2 의 발현에 관한 면역조직화학적 연구

김경달(Kyung Dal Kim),김현진(Hyun Jin Kim),김낙인(Nack In Kim) 대한피부과학회 2002 대한피부과학회지 Vol.40 No.5

Background : The pathogenesis of psoriases remains upcertain. It is konwn that a variety of factors take a role in its pathogencsis. One of them is the lteration of keratimocytes differentiation. The terminal differentiation of kerationocyles includes the process of the synthesis of proteins such as involucrin, filaggrin and cornifin, and produce keratohyaline granules and a structure terned cornified cell envelope finally. And the terninal differentiation of keratinocytes is konwn as a process of apoptosis, proguamned cell death . Objective : In this study, we tried to clarify the pathogenctic mechanisms of psoriasis by comparing the expression patterns of several proteins(involuerin, loricrin, filaggrin) associated with keratinocyte differentiation and of bel-2 protein, known as inhibitor of apoprosis, between lesional and non-lesional psoriatic skin. Results : The results were summarized as follows, firstly early expression of involuerin in lower epidermis, secondly on or reduced expression of filaggrin and lorierin in upper epidermis and lastly no expression of bel-2 in basal layer of psoriatic skin. Conclusion : This study clarified that the accelerated terminal differentiation, the shortening of cell-cycle of keratinocyles, and the increased trunover of gerationcytes may be involved in the pathogenetic role of psoriasis.

원형 탈모증 환자의 말초혈액내 면역세포의 변화에 관한 연구

임성춘(Sung Choon Lim),김낙인(Nack In Kim),허충림(Choong Rim Haw) 대한피부과학회 1986 대한피부과학회지 Vol.24 No.1

Authors have quantitated the T cell and T subsets(T and T cell) in the per- ipheral blood of 16 patients with alopecia areata and 16 normal healthy controls. The results are as follows: 1. The mean values of the T cell in total patient group(54,6+7.9g) and AAP group(5,3. 1+8. 1%) showed staticstially significant decrease when it compared with the mean value of the control group(64.6+3.6%) respectively(p<0.005). In ATU group the mean value of the T cell(53. 1+8. 1%) also showed decreasing tendency in comparison with that of control. The mean values of the T cell in total patient group(30.9+8. 9%) RIld AAP group(3'l. 5+8. %) showed statistically significant decrease when it compared with the mean value of the control group(42,6+8.9%) respectively(p<0. 005). In ATU group the mean value of the T< cell(28. 7+10. 9%) also showed decreasing tende- ncy in comparison with that of control. 3. The mean values of the Tr, cell in total patient group(6. 1+2. 2%) and AAP group(5.9+2.4%) showed staticstially significant decrease when it compared with the mean value of the control group(7.9+1.3%) respectively(p(p pl, pgp p25) In ATU group the mean value of the T cell(6. 7+0. 9%) also showed decreasing tendency in comparison with that of control. 4. The mean values of the T/T ratio showed no significant difference between the patient groups(total, AAP, ATU) and control group, respectively. Above data showed that defect of T cell and T subsets may be associated with the pathogenesis of alopecia areata.

한국 청소년에서의 Piezogenic papules에 대한 임상적 고찰

이승한,이무형,심우영,김낙인,허충림 ( Seung Han Lee,Mu Hyoung Lee,Woo Young Sim,Nack In Kim,Choong Rim Haw ) 대한피부과학회 1991 대한피부과학회지 Vol.29 No.5

A Clinical Study of Piezogenic Papules in Korean Youth Seung Han Lee, M.D., Mu Hyoung Lee, M.D., Woo Young Sim, M.D., Nack In Kim, M.D., Choong Rim Haw, M.D. Department of Dermatology, College of Medicine, Kyung Hee University Seoul, Korea We investigated the prevalence, distribution, the number and size of the piezogenic papules in a student population, aged 15 to 25 years old. The results were as follows ; 1. The prevalence was 7.4%(86 cases/1167 students in total). 2. The prevalence in male was 4.6% (43/944), and that in female was 19.3%(43/223)(P<0.001). 3. The number of patients who had piezogenic papules on one foot was 48(55.8%), and those who had lesions bilaterally was 38(45.2%), Lesions of the left foot were more common than right. The medial aspect of the heel was 6 to 10 times a more common location compared to the lateral or posterior aspect(P<0.05). 4. The average number of lesions was 4.5 and there was no significant difference between male and female, and right and left foot. The number of papules on the medial aspect of the heel was 3 times more than that of the lateral, or posterior aspect(P<0.05). 5. The average size of lesions is 4㎜ in diameter(2㎜-10㎜), and of the patients were asymptomatic. 6. The average body weight of patients, 64.5±8.5kg in the male and 55.5±8.8kg in the female, was greater than those of the control group, 62.9±8.5kg and 52.5±5.0kg, respectively, but there was no statistically significant differenceir=0.150).

방사선 조사가 배양된 인체 각질형성세포 및 멜라닌세포에 미치는 영향

유한동(Han Dong Yoo),김낙인(Nack In Kim),박재경(Jai Kyung Park),홍성언(Seong Eon Hong),허충림(Choong Rim Haw) 대한피부과학회 1994 대한피부과학회지 Vol.32 No.4

Background : Radiation has been used in t,he medical field of dragnosis and treatment. There is widely used ionizing radiat:ion such as naturally occuring r-rays or machine-made X-ray. This radiation is able to induce the structural and functional alterations of the mammalian cells. But we have few detailed reports on the effects of radiation on epidermal cells and their immune functions. Objective : This study was performed to investigate the effect of radiation on cultured human keratinocytes and melanocytes. Materials and Methods : Cultured human keratinocytes and melanocytes were irradiated with 2,6, l0Gy from a Co saurce and stimulated by 100 U/ml of ekratinocyte immediately after irradiation. We investigated cell numbers and morphological changes, DNA synthesis and HLA-DR antigen expression. Results : After exposure to r-ray, the proliferation of keratinocytes and melanocytes decreased in a time and dose dependent fashion to each control group. Tliey showed decreased density, a larger size and a round appearance after radiation exposure and an especially shortened and decreased number of dendrites in the melanocytes. In DNA synthesis counted using [H]-thymidine incorporation, the keratinocvtes decreased values in a dose depen(lent manner at 24 and 72 hours after irradiation but no differense was observed at 168 hours. In melanocytes, there was a greater decrease than that of keratinocytes. The melanin content/cell in all radiation exposed groups increased in a time and dose dependent fashion t,o each contr ol group. HLA-DR antigen expression on keratinocytes after radiat,ion exposure decreased to the control group, but there were no significant differences acccirding to the dose of radiation, And there were no significant diifferences of HLA-DR antigen expression on the melanocytes betweer. controls and the radiation exposed groups. Conclusion : Antiproliferative activity was dependent on the exposure time and dose of r-ray exposure. According to the time after radiation exposure, melanogenic activity was stimulated. The expression of HLA-DR, antigen decreased in keratinocyte after radiation exposure but there was no decrease in melanocytes. (Kor J Dermatol 1994:32(4); 609-619)

아토피피부염 환자의 혈장 히스타민 농도

백종현(Jong Hyun Paik),이무형(Mu Hyoung Lee),김낙인(Nack In Kim),허충림(Choong Rim Haw) 대한피부과학회 1999 대한피부과학회지 Vol.37 No.11

Background:Although there is evidence to support histamine involvement in atopic dermatitis, the role of histamine in pathogenesis of atopic dermatitis and the development of pruritus have been discussed controversially. Objective:The purposes of this study were to investigate the plasma histamine levels in patients with atopic dermatitis of different severity and to evaluate the correlation between plasma histamine levels and other parameters of atopy, such as, basophil counts, eosinophil counts, and total IgE levels. Methods:We measured plasma histamine levels, serum total IgE levels, peripheral basophil counts, and peripheral eosinophil counts in twenty patients with atopic dermatitis and in seventeen normal control subjects. Results:1. There was no significant difference in plasma histamine levels between patients with atopic dermatitis and normal control subjects. 2. A comparison of atopic dermatitis patients with increased plasma histamine levels and those with normal histamine levels revealed no difference regarding age, sex, and serum IgE levels ; however, the group with increased plasma histamine levels was associated with other atopic disease, more commonly. 3. There were no correlation between the clinical severity of atopic dermatitis, seveirty of itching in patients with atopic dermatitis and the plasma histamine levels. 4. Plasma histamine levels in atopic dermatitis were correlated with basophil counts and eosinophil counts. However, there was no correlation between the plasma histamine levels and the serum total IgE levels. Conclusion:There was no significant difference of plasma histamine levels between patients with atopic dermatitis and normal control subjects. However, further investigations for measurement of histamine level after stimulation with variety of different substance may lead to clarification of evidence for histamine release in atopic dermatitis.

정상인 및 나환자 표피 각질층의 수분 함량에 관한 연구

심우영(Woo Young Sim),김낙인(Nack In Kim),허충림(Choong Rim Haw),임수덕(Soo Duk Lim) 대한피부과학회 1985 대한피부과학회지 Vol.23 No.5

We evaluate the water content, hygroscopicity and water holding capacity in 40 healthy individuals and 17 leprosy patients by skin surface hydrometer(IBS Inc, Japan), and the correlation between the nerve destruction and the hydration state. In Eiealthy individuals the measuring sites were the flexor and extensor surfaces of the arm, forearm, thigh and leg, In leprosy patients we measured on the extensor surface of forearm with shows abnormal sensation, and opposite ar neighboring skin which shows normal sensation. The results are as follows: 1. The water content of healthy individuals is highest in the flexor surface af arm (49. 50 + 18.38ug ) and lowest in the extensor surface of leg(30.75 -12.09ug) 2. The hygrosccpicity of healthy individuals is highest in the flexor surface of arm(296. 30 + 70.75ug ) and lowest in the extensor surface of leg(24$. Q7+5) 5ppg) 3. The water content of the extensor surface of lorearm in leprosy patients, which .hows abnormal sensation, is decreased(16. 35 +20. 82 ug) and it is statisti calli significant when compared with healthy individuals(p<0.005). 4. The hygroscopicity of the extensor surface of forearm in leprosy patients. which shows abnormal sensation, is decreased(94. 29 + 71. 62 ug) and it is statistically significant when compared with opposite or neiphboring skin and healthy individuals(p<0. 005)

모발로 분화하는 종양에 대한 임상 및 병리조직학적 소견 -대한피부과학회 피부병리 연구분과위원회 공동연구-

김유찬 ( You-Chan Kim ),강원형 ( Won-Hyoung Kang ),고재경 ( Jai-Kyoung Koh ),김낙인 ( Nack-In Kim ),김방순 ( Bang-Soon Kim ),김상원 ( Sang-Won Kim ),김수남 ( Soo-Nam Kim ),김수찬 ( Soo-Chan Kim ),노영석 ( Young-Suck Ro ),명기범 ( 대한피부과학회 2003 대한피부과학회지 Vol.41 No.9

Background : Data on the clinicopathologic features of hair follicle tumors in Korea are limited. Objective : The purpose was to investigate the clinical and histopathologic characteristics of hair follicle tumors in Korea. Methods : Two hundred sixty four cases of hair follicle tumors seen from 1999 to 2001 in Korea were analyzed clinically and histopathologically. Results and Conclusion : 1. The most common hair follicle tumor in Korea was pilomatricoma(81.0%), followed by trichoepithelioma(6.4%), dilated pore of Winer(3.8%), and proliferating trichilemmal tumor(2.2%). 2. Hair follicle tumors usually occurred as a solitary skin-colored nodule. The most commonly involved site was the face, and the peak age of presentation was 10 to 19. 3. Pilomatricoma usually occurred as a solitary skin-colored nodule. The most commonly involved site was the arm, and more than 50% of tumors occurred before the age of 20. Histopathologically, the tumor was often surrounded by fibrous capsule and was composed of basophilic and shadow cells. It was usually located in the dermis and extended into the subcutis. Retraction spaces between tumor nests and stroma were frequently observed. 4. Trichoepithelioma usually occurred as multiple skin-colored papules on the face. Histopathologically, the tumor was usually located in the dermis with sometimes connected to the epidermis. It frequently showed peripheral palisading. 5. Dilated pore of Winer usually occurred as a solitary brownish to pigmented papule on the face in middle-aged persons. 6. Proliferating trichilemmal tumor occurred as a solitary or multiple tumors. Histopathologically, the tumor was usually surrounded by incomplete fibrous capsule. Tumor cells frequently showed clear cell formation, nuclear atypia, mitosis, peripheral palisading, trichilemmal keratinization, and individual keratinization. (Korean J Dermatol 2003;41(9) : 1177~1186)