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간경변증 및 뇌질환 환자의 혈장 APV의 측정과 수분대사에 대한 연구
김극배 순천향대학교 1984 논문집 Vol.7 No.1
In order to studying the water balance in brain disease and liver cirrhosis. 15normal control subjects, 10 patients of liver cirrhosis and 20 patients of brain disease were subjected to determine plasma AVP levels by sensitive radioimmuno assay and simultaneous determined plasma and urine osmolality, Results were. 1. Plasma AVP concentration in raodomly hydrated normal adult control was average 2.0 pg/ml with range of 0.4~5.6 pg/ml. Results was fairly correlated the serum osmolality and osmotic threshold was 280.4 mOsm/kg. 2. Plasma AVP level in 10 patient of liver cirrhosis was average 2.8pg/ml. with range of 0.2~9.0 and plasma and urine osmolality ratio was marked decrease in compare with normal subjects and poorly corrlated with plasma AVP and plasma osmolality. 3. Plasma AVP level in 20 patients of brain disease was average of 0.4~9.0pg/ml. There are no difference of plasma level in average concentration with normal control but marked fluctuation between patients. And also poorly correlated with plasma AVP and plasma osmolality. 4. There are negligible AVP concentration and marked decreased U/P ratio in two cases of polyuric patients. 5. Studies suggested there are more complex osmotic, nonosmotic stimuli and inhlbitory stimuli affected in liver cirrhosis and brain disease.
폐경기전후 한국 여성의 비타민 - D 수용체 유전자의 Restriction Fragment Length Polymorphisms 과 골밀도 및 골표지자와의 관계에 대한 고찰
김극배,한인권,서교일,변동원,김상우,유명희,문인걸 대한내분비학회 1995 Endocrinology and metabolism Vol.10 No.3
Osteoporosis is now a major health problem because of the increasing elderly population and related osteoporosis fractures. Recently, it has been suggested that lower bone mass with/and high bone turnover rate is considered to be important in the developing of osteoporosis, and so there has been many efforts to identify the risk factors which is considered to cause lower bone mass and high bone turnover. Osteocalcin, the most abundant noncollagenous protein in bone, is a marker of bone turnover and its synthesis is induced by calcitriol(the active form of vitamine-D) through the vitamine-D receptor(VDR) and a specific vitamine D-responsive element in the osteocalcin gene promoter. Serum concentrations of osteocalcin are under the strong genetic influences and may reflect allelic variation in VDR gene. Therefore, the present study were designed to find the relationships among the polymorphisms of Vitamine-D receptor gene, bone mineral density and bone markers. We analysed the restriction fragment length polymorphisms of VDR gene with Bsm I endonuclease enzyme in relation to bone mineral density by using DEXA(dual energy X-ray absorptiometry, QDR-2000) and bone markers, especially serum osteocalcin concentrations in 356 pre- and postmenopausal Korean women. The frequence of RFLPs of VDR gene is 3.3% in BB type, 10.1% in Bb type, 86.6% in bb type. The concentrations of osteocalcin, alkaline phosphatase, procollagen-C and urinary deoxypyridinoline/creatinine were found to be higher in postmenopausal than premenopausal women and the levels of BMD were lower in postmenopausal than premenopausal women. The BB type, which is known to have a strong genetic determinant, is less frequently encountered in Korean women and does not correlate with levels of bone markers and bone mineral density. Even though the number of women with BB type is small, we noted the mean serum level of each bone marker was greater in postmenopausal women with BB type than in premenopausal women with the same genotype. In conclusion, this may suggest a partial agreement of our data with that of Australlian group and that we have to try to find out another genotype specifically related with lower bone density in Korean women(J Kor Soc Endocrinol 10: 249-261, 1995)
정상인 및 인슐린 비의존성 당뇨병 (NIDDM)환자에서 고성능 인슐린 친화 칼럼을 이용한 적혈구 인슐린 수용체의 분리에 관한 연구
김극배,유명희,이희발 대한내분비학회 1991 Endocrinology and metabolism Vol.6 No.4
Insulin receptor defect is thought to play a role in the pathogenesis of NIDDM. C-DNA cloning of insulin receptor has contributed to the identification of several structural defect of the insulin recepotr in patients with extreme insulin resistance. But the site of insulin receptor defect in NIDDM has not been determined unequivocally due to polymorphism in RFLP analysis of the insulin receptor gene. Also low yield of insulin reeptor protein through the purification process made it difficult to analysis the insulin receptor protein in NIDDM. We purified RBC insulin receptor in normal control and NIDDM with the use of Con A-agarose column (Bio-Rad, USA) to get glycopeptide and high performance insulin affinity column to purify insulin receptor. 12II -insulin binding activity was evaluated in each fraction of the chromatogram, and the results were as follows. 1) RBC insulin receptor was concentrated more than 500 folds though Con A-agarose column. 2) RBC insulin receptor purified by Con A-agarose column was concentrated more than 10 folds through high performance insulin affinity column. 3) RBC insulin receptor was concentrated more than 5000 folds by Con A-agarose and high performance insulin affinity column, and the recovery of 125I -insulin binging activity was 60% in Con A-agarose and 90% in high performance insulin affinity column. We suggest that such improvements in the yield of the purification of RBC insulin receptor will contribute to the future analysis of insulin receptor protein in NIDDM. (J Kor Soc Endocrinol 6:308~313, 1991)