Chitosan - g - Poly ( 4- vinyl pyridine ) 공중합체막을 통한 인슐린의 투과 특성

김공수,강석호,김수종,임정규 ( Kong Soo Kim,Seog Ho Kang,Soo Jong Kim,Jeong Kyu Lim ) 한국공업화학회 1994 공업화학 Vol.5 No.4

Chitosan-g-Poly(4-vinyl pyridine)공중합체막을 제조하였으며 Chitosan-g-Poly(4-vinyl pyridine)공중합체막과 아크릴아마이드를 사용하여 glucose oxidase를 포함하는 chitosan/acrylamide 복합막을 제조하였다. Chitosan-g-poly(4-vinyl pyridine)공중합체막의 함수율과 인슐린의 투과량은 용액의 pH가 감소함에 따라 증가하였고, 복합막의 인슐린 투과량은 글루코오스의 농도가 증가할수록 증가하였다. Chitosan-g-poly(4-vinyl pyridine)membranes were prepared by crosslinking reaction using glutaraldehyde and glucose oxidase immobilized chitosan/acrylamide composite membranes were fabricated by chitosan-g-poly(4-vinyl pyridine) copolymer and acrylamide. Water content and permeability of insulin through chitosan-g-poly(4-vinyl pyridine)membranes increased with decreasing the pH of the medium Permeability of insulin through chitosan/acrylamide composite membranes increased with increasing the concentration of glucose.

스틸렌 - 아크릴산계 공중합체의 금속착물 형성에 관한 연구

김공수,김수종,조석형,전용철 ( Kong Soo Kim,Soo Jong Kim,Suk Hyeong Cho,Yong Chul Chun ) 한국공업화학회 1991 공업화학 Vol.2 No.1

수용성 poly(sulfonated styrene-co-acrylic acid)를 합성하여 Cu(II)이온과의 착물형성에 관한 연구를 수행하였다. poly(sulfonated styrene-co-acrylic acid)-Cu(II)착물의 생성반응에 있어서 pH 변화에 따른 UV-Vis.흡수 스펙트라 및 점도 특성을 시험하여 착물형성조건을 검토하였다. 또한, poly(sulfonated styrene-co-acrylic acid)-Cu(II) 착물의 생성상수와 안정도상수를 Bjerrum 방법으로 구하였고 Ringbom 방법으로 착물생성 엔탈피, 자유에너지 및 엔트로피 변화 등 열역학적 특성값을 구하였다. Water soluble poly(sulfonated styrene-co-acrylic acid) was polymerized with sulfonated styrene and acrylic acid in the presence of silver sulfate at 99 ℃ for 4 hrs. The complex formation of poly(sulfonated styrene-co-acrylic acid) with Cu(II) was carried out. The maximum absorption wavelength of the poly(sulfonated styrene-co-acrylic)-Cu(II) system at different pH values was observed at 274 nm and 295 nm. The reduced viscosity of the poly(sulfonated styrene-co-acrylic acid)-Cu(II) complex were measured in the various pH ranges. The formation constants and stability constants of poly(sulfonated styrene-co-acrylic acid)-Cu(II) complex were calculated from Bjerrum method. The changes of enthalpy, free energy and entropy in the above reaction were determined by Ringbom method.

허혈성 자극에 의한 심방이뇨 호르몬 분비 반응의 특성

김공수,김민호,김창곤,김석기,조경우,최훈,Kim, Kong-Soo,Kim, Min-Ho,Kim, Chang-Gon,Kim, Suk-Kee,Cho, Kyung-Woo,Cui, Xun 대한흉부심장혈관외과학회 2000 Journal of Chest Surgery (J Chest Surg) Vol.33 No.5

Background: Cardiac atrium is an endocrine gland secreting a family of natriuretic peptides. The secretion of atrial natriuretic peptide(ANP) had been shown to be controlled by variable factors. The change in atrial dynamics have been considered as one of the most prominent stimuli for the stimulation of ANP secretion. Hypoxic stress has been shown to increase cardiac ANP secretion. However, the mechanism by which hypoxia increases ANP secretion cardiac ANP secretions. However, the mechanism by which hypoxia increases ANP secretion has not to be defined. Therefore, the purpose of the present study was tow-fold: to develop a protocol to defined the effect of hypoxia on ANP secretion in perfused beating rabbit atria and to clarify the mechanism responsible for the accentuation by hypoxia of ANP secretion. Material and Method: Experiments have been done in perfused beating rabbit atria. ANP was measured by radioimmunoassay. Result: Hypoxic stimulus with nitrogen decreased atrial stroke volume. The decrease in atrial stroke volume recovered basal level during the period of recovery with oxygen. ANP secretion and the concentration of perfusate ANP in terms of extracellular fluid(ECF) translocation which reflects the rate of myocytic release of ANP were increased by hypoxia and returned to basal levels during the recovery. Changes in ECF translocation paralleled by hypoxia and returned to basal levels during the recovery. Changes in ECF translocation paralleled to that of atrial stroke volume. At the start of recovery in atrial storke volume, ECF tranalocation incrased for several minutes. The above responses were stable and reproducible. Glibenclamide treatment prevented the recovery in atrial stroke volume. Increments by hypoxia of ANP secretion and ANP concentration were suppressed by glibenclamide. Conclusion: These results indicate that hypoxia incrased atrial myocytic ANP release and that the mechanism responsible for the accentuation is partially related to the change in K+ATP channel activity.

다공성 고분자 마이크로겔의 Bovine Serum Albumin 단백질의 흡착평형

김공수,강석호 ( Kong Soo Kim,Seog Ho Kang ) 한국공업화학회 1998 공업화학 Vol.9 No.2

물리·화학적인 특성이 다른 다공성 고분자 마이크로겔에 대한 bovine serum albumin(BSA-protein) 단백질의 흡착평형 특성을 연구하였다. 수용액 속에서 고분자와 단백질사이의 소수성 상호작용에 의하여 폴리부틸메타크릴레이트(PBMA) 마이크로겔이 폴리비닐피리딘 (PVP)과 플리아크릴로니트릴 (PAN) 마이크로겔보다 높은 흡착특성을 나타내었으며, PBMA 마이크로겔이 PVP와 PAN 마이크로겔보다 비가역적으로 흡착평형 특성을 나타내었다. 그러므로 고분자 마이크로겔의 물리적인 특성과 단백질-고분자 마이크로겔 사이의 정전기적 인력보다는 소수성 상호작용이 단백질의 흡착특성에 중요한 역할을 하고 있음을 알 수 있다. 또한 PBMA, PVP 및 PAN 마이크로겔 모두 Freundlich 흡착 등온식보다는 Langmuir 흡착 등온식에 잘 적응되었다. The adsorption equilibrium properties of bovine serum albumin(BSA-protein) for three kinds of porous microgels with different physical and chemical features were investigated. The adsorption amount of BSA-protein on poly(butyl methacrylate) (PBMA) microgels was higher than those on poly(vinyl pyridine) (PVP) and poly(acrylonitrile) (PAN) microgels due to the hydrophobic interaction between polymer and protein in an aqueous solution. And PBMA microgels had more irreversible adsorption equilibrium properties than PVP and PAN microgels. It implies that hydrophobic interaction plays a more important role in adsorption properties of BAS-protein than physical properties of polymer and electrostatic attraction between protein and polymer microgels. Characteristics of the microgels used in this study followed Langmuir equation better than the Freundlich equation.

단분산성 다공의 폴리아크릴로니트릴 - 에틸렌 글리콜 디메타크릴레이트 마이크로 겔의 제조 및 특성

김공수,강석호,김영식 ( Kong Soo Kim,Seog Ho Kang,Young Sik Kim ) 한국공업화학회 1996 공업화학 Vol.7 No.4

Seed 중합 방법에 의하여 단분산성 다공의 폴리아크릴로니트릴-에틸렌글리콜 디메타크릴레이트 (PAN) 고분자 마이크로 겔이 제조되었다. PAN 마이크로 겔의 물리적인 특성을 모노머/seed와 희석제/모노머의 비율, 가교제의 농도 및 희석제의 종류에 따라 연구하였다. PAN 마이크로 겔의 입도 분포는 모노머/seed의 비율이 50 이하 일 때 단분산성을 나타냈으며, 가교제의 농도가 증가할수록 입자 내부의 미소 구체 (microsphere)의 크기가 작아져 더욱 밀집된 형태를 나타내기 때문에 입자의 비표면적이 증대되었다. 또한 희석제로 톨루엔을 사용하고 희석제/모노머의 비율이 1.0일 때 입자표면의 세공이 가장 발달되었다. Uniform sized-porous poly(acrylonitrile-ethylene glycol dimethacrylate) (PAN) microgels were synthesized by seed polymerization using monodisperse polystyrene seed particles. The effect of weight ratios of monomer/seed (M/S) and diluent/monomer (D/M), concentration of crosslinking agent and the type of diluent was investigated on the formation of PAN microgels. The particle size distribution of PAN microgels was found to be monodisperse in that the weight ratio of M/S is less than 50. More porous microgels were formed and the interstices between small sized interior microspheres present inside of PAN microgels were also smaller with increasing crosslinking agent concentration. Well-developed pore structure was found to be in that weight ratio of D/M is 1 and toluene is used as a diluent.

CA 막에 4-비닐피리딘의 그라프트 공중합 및 그의 페놀 투과특성

김공수,이민석,박준하,김영식 ( Kong Soo Kim,Min Suk Lee,Jun Ha Park,Young Sik Kim ) 충북대학교 산업과학기술연구소 1996 산업과학기술연구 논문집 Vol.10 No.1

Abstract_Roman Graft copolymerization of 4-vinylpyridine onto CA membrane was carried out in an aqueous medium by using ceric ammonium nitrate(CAN) as an initiator. The optmium condition for the graft copolymerization were 60'C; pretreatment and grafting

생분해성 Microgel 로부터 약물을 담지한 Microsphere 의 제조 및 그의 생분해성

김공수,강석호,김정묵 ( Kong Soo Kim,Seak Ho Kang,Jeong Muk Kim ) 한국공업화학회 1998 공업화학 Vol.9 No.4

Dipping 법에 의한 천연고무와 그라프트 라텍스 블렌드 필름의 표면특성

김공수,박준하,엄주송 ( Jun Ha Park,Kong Soo Kim,Ju Song Eum ) 한국공업화학회 1994 공업화학 Vol.5 No.6

천연고무 라텍스(NRL)와 메틸메타 크릴레이트가 그라프트된 라텍스(MGL)를 각종 첨가제와 혼합하여 dipping 공정으로 가황된 NR 및 블렌드 필름을 제조하였다. NR 필름 제조의 최적조건을 규명하기 위하여 숙성시간에 따른 팽윤도, 인장강도 및 110℃에서 가황시간의 변화에 따른 기계적 특성을 비교하였다. 필름 표면의 구조 및 미끄럼성을 조사하기 위하여 접촉각 및 정·동마찰계수를 측정한 결과, 접촉각은 MGL의 블렌드 비율이 증가할수록 감소하였고, 정 및 동마찰계수는 NR 필름에 비하여 NR/MG 과 NR-d-MG 필름이 현저히 감소하여 표면 미끄럼성이 향상됨을 알 수 있었다. The vulcanized NR and blend films were prepared with mixing of natural rubber latex (NRL) and methyl methacrylated grafted latex(MGL) with various additives by dipping process. It was investigated the basic properties of vulcanized NR films that is optimum condition of the mature time, swelling degree, cure time at 110℃, and measured the mechanical properties of tensile strength and elongation of its condition. In order to identify the surface structure and the slip properties of blend films, contact angles and static and kinetic friction coefficient were measured. Contact angles were decreased with increment of blend ratio of MGL, and static and kinetic friction coefficient were decreased rapidly for the NR/MG and NR-d-MG films than for the NR films. From the results, NR/MG and NR-d-MG films has slip`s reinforcement in skin contact surface with increased of blend ratio of MGL.

NR / CR 라텍스 블렌드 필름의 제조 및 물리적 특성

김공수,박주하,엄주송 ( Kong Soo Kim,Jun Ha Park,Ju Song Eum ) 한국공업화학회 1996 공업화학 Vol.7 No.4

천연고무 라텍스(NRL)와 각종 첨가제를 배합 숙성하여 NR 필름을 제조하였고, 클로로프렌고무 라텍스(CRL)를 일정한 비율로 블렌드하여 NR/CR 필름을 제조하였다. 이들 필름의 전가류 시간에 따른 팽윤도와 기계적 성질의 변화를 측정한 결과, 팽윤도 80∼85%, 전가류 시간 48∼60hrs등에서 가장 우수하였고, CRL의 블렌드 비율이 증가함에 따라 인장강도는 감소하고 신장율은 증가하는 경향을 나타내었다. 제조공정을 달리하여 제조한 시편의 기계적특성을 비교한 결과, 1회 디핑법을 사용한 NR/CR 필름의 인장강도, 인열강도는 2회 디핑한 NR-d-CR 필름보다 더 우수하였다. 이들 시편의 표면을 SEM으로 관찰한 결과, CRL의 블렌드 비율이 증가할수록 필름 표면의 벌크한 집합체가 증가되는 것으로 보아 상분리가 일어났음을 관찰할 수 있었다. The NR films were prepared with mixing of natural rubber latex(NRL) with various additives, and NR/CR films were prepared by blend ratio of chloroprene rubber latex(CRL). The swelling degree and the mechanical properties of these films were measured according to the precure time. As a result, optimum condition were showed the swelling degree : 80∼85%, precure time : 48∼60hrs., and tensile strength was reduced but elongation was increased as increasing the blend ratio of CRL. On the mechanical properties of films prepared by different dipping process in these optimum condition, the tensile strength and tear strength of NR/CR films by one dipping process are better than NR-d-CR films by two dipping process. The surface of these films were observed with scanning electron microscopy(SEM). It was found that phase separation was occured as increasing the blend ratio of CRL.

폴리스티렌겔내에서 침전중합법에 의한 Poly ( AA - co - EGDMA ) Microgel 의 합성

김공수,조병호,조석형 ( Kong Soo Kim,Byung Ho Cho,Suk Hyeong Cho ) 한국공업화학회 1996 공업화학 Vol.7 No.2

Poly(AA-co-EGDMA) microgel은 시크로헥산/아세톤용액에 팽윤된 폴리스티렌겔 용액중에서 침전중합 방법으로 합성하였다. Microgel을 합성함에 있어서 폴리스티렌의 농도, 가교제의 농도, 폴리스티렌과 모노머의 비율 및 용매의 성질에 따라 0.18∼0.55㎛의 입자크기로 조절할 수 있었으며, 구형의 단분산성 microgel을 합성할 수 있었다. 또한 폴리스티렌 용액의 농도가 증가함에 따라 microgel의 입자크기는 작아졌고, 가교제의 농도가 증가할 수록 입자크기는 증가하였다. Poly(AA-co-EGDMA) microgels were prepared by precipitation polymerization in highly swollen polystyrene gel solution. The polymerization was carried out in selective solvent such as cyclohexane/acetone at 60℃. Poly(AA-co-EGDMA) microgels with various size of 0.18∼0.55㎛ were obtained from different concentration of polystyrene(PS), concentration of crosslinking agent(EGDMA), ratio of PS/monomer and volume ratio of solvents. The particle size of poly(AA-co-EGDMA) microgels decreased with increasing the concentration of PS and increased with that of EGDMA.