한국인삼의 항종양 효과 : 세포독성과 종양의 성장억제에 미치는 영향(1) Cytotoxicity and Tumor growth delay(1)

권형철,김정수 의과학연구소 1996 全北醫大論文集 Vol.20 No.1

Purpose : To investigate the effect of aueous extract of Korean Ginseng(K.G) on the survival of tumor cells in vitro and on the growth of tumors in vivo. Materials and Methods : Dried K.G. roots were made into powder, extracted with distilled water, filitered and diluted from a maximum concentration of 100mg/ml in sequence. The cytotoxicity of K.G. in vitro was evaluated from its ability to reduce the clonogenicity of SCK tumor cells. For the tumor growth delay study, about 2X10exp(5) of SCK tumor cells were subcutaneously inoculated in the legs of A/J mice. the first experimental group of mice were injected intraperitoneally(i.p.) with 0.2ml of 500mg/kg of K.G. from the first day after tumor inoculation for 10 days. The second experimental group of mice were injected I.p. with 0.2ml of 500mg/kg of K.G. twice a day for 5 days beginning from the 7th day after tumor inoculation. Results : (1) Cytotoxity in vitro ; survival fraction, as judged from the curve, at K.G. concentration of 1, 5, 10, 25, 50 and 100mg/ml were 1.0±0.02, 0.58±0.07, 0.84±0.03, 0.25±0.02 0.004 and 0.002 respectively. In the concentration-cell survival fraction relationship, the range of ginseng concentration at 10% of cell surbibal fraction was 25~50mg/ml. (2) Tumor growth delay in vivo : 1) the time required for the mean tumor volume to grow to 1,000㎣ was 11 days in the control group and 12 days in the experimental group(p>0.05). 2) the time required for tumor volume to increase 4 times was 11 days in both groups. Conclusion : Aqueous extract of dried K.G. roots showed a marked cytotoxicity on the SCK mammary cellls in vitro and te range of ginseng concentration at 10% cell survival fraction was 25~50mg/ml. When K.G. injection was started soon after tumor inoculation, tumor growth was delayed than that of control group, but it was not significant. When injection was started after the tumors were firmly established, difference of tumor growth delay was not appeared in both group.

광대역CDMA 복조기의 코드 추적 및 복조 회로 설계 ( Design of a code tracking and demodulation circuit for wideband CDMA demodulator )

권형철,오현서,김현철,조경록 대한전자공학회 1998 대한전자공학회 학술대회 Vol.1998 No.01

租稅特例制度에 대한 硏究

권형철 서울市立大學校 經商大學 稅務學科 2000 稅務學論集 Vol.13 No.1

IMT-2000 광대역 CDMA의 동기추적 및 데이터 복조 회로구현

권형철,오현서,이재호,조경록 한국통신학회 1999 韓國通信學會論文誌 Vol.24 No.6

본 논문은 광대역 CDMA 방식의 IMT-2000 단말기용 이동국의 PN 코드 위상 동기를 위한 추적기와 데이터를 복원하는 복조기회로 설계 및 구현에 대해서 기술한다. 먼저 동기 추적을 위한 회로는 넌코히어런트 방식을 사용하여 설계하였으며 동기 추적 과정에서 발생되는 클럭이 1/8 PN 칩의 해상도를 갖도록 설계하였다. 복조기 부분은 코히어런트 방식을 사용하여 설계하였으며 타임 트래킹 동작에 의해서 발생되는 클럭으로 생성된 PN 코드와 수신신호를 역확산하여 원래의 데이터를 복원하도록 설계하였다. 32.786 MHz의 구동 클럭과 4.096 MHz의 칩율을 사용하였으며 FPGA로 구현하였다. 또한 설계된 복조기는 32Kbps 음성 및 신호 채널에서 정상 동작함을 확인하였다. 성능 검증을 위하여 AWGN(Additive White Gaussian Noise) 7dB로 시뮬레이션하여 데이터 복원이 이루어 졌으며 무선 가입자망(WLL:wireless local loop)과 IMP-2000 변복조기 설계에 적용할 수 있음을 알 수 있었다. In this paper, a pseudo-noise(PN) tracking and demodulation circuits are analyzed and designed for a direct-sequence/spread-spectrum multiple access system under a mobile fading channel. We consider noncoherent delay locked loop(DLL) as a PN code tracking loop which has 1/8 PN chip resolution. The tracking performance of DLL is evaluated in terms of locking time from a loose state and tracking jitter. The received signal is demodulated to original data by despreading with PN code locked by DLL. Also the designed circuit supports sound service of 32Kbps and in-band signal with 4.096MHz chip clock. The circuits are implemented and verified with FPGA, which is shown completely data recovery under AWGN 7dB and will be available for IMT-2000.

악성 피부종양의 방사선치료 : 피부종양 16예에 대한 분석 A study of skin tumor in 16 cases

권형철,윤세철,박용휘 대한암학회 1987 Cancer Research and Treatment Vol.19 No.1

방사선치료 중 초래되는 백혈구 감소증 치료에 사용되는 세파란친의 약물효과

권형철,김형진,김정수,김종훈,양두현 의과학연구소 1994 全北醫大論文集 Vol.18 No.2

Purpose : To investigate the hematopoietic effectiveness of cepharanthine on the treatment of leukopenia caused by radiation therapy(RT). Materials and Mdthoes : 14 patients who developed leukopenia during RT entered into trial. 10 of 14 patients took cepharanthine during RT(observed group) and 4 patients only received RT without cepharanthine treatment(control group). All patients received external irradiation as standard fractionation of daily 180~200cGy in 5 days per week. Oral intake of cepharanthine was started when leukocyte counts decreased below 4,000, continued until the end of RT. Daily dosage was 6mg. Drug effect was evaluated as effective and no effective response according to change and recovery time(before 3 weeks after RT or less than 3,000 cGy) of leukocyte counts. Results : (1) Changes in leukocyte counts ; 1) Control group ; All patients appeared decrease of leukocyte counts from 1,000 cGy and recovery after 3,000 cGy. 2) Observed group ; Five of ten patients showed consistent increasing tendency and recovery before 3,000 cGy, One showed consistent increasing tendency but recovery appeared after 3,000 cGy, Four showed incosistent increasing tendency or no change or decreasing tendency, respectively, (2) Changes in platelet counts ; 1) Control group ; All patients appeared decrease of platelet counts from 1,000~2,000 cGy and recovery after 3,000~4,000 cGy. 2) Observed group ; Three of ten patients showed consistent or inconsistent increasing tendency after intake of cepharanthine. Five showed decreasing tendency and two already showed increasing tendency before intake of cepharanthin. (3) Drug effect ; 5 of 10 patients showed effective response, 5 showed no effective response. (4) Comlication ; No toxicity was encounterd. Colclusion : Our results suggest that cepharanthine is a effective and safe drug on treatment of leukopenia caused by RT.

Dual-frequency Capacitively Coupled Plasma-enhanced Chemical Vapor Deposition System for Solar Cell Manufacturing

권형철,원임희,신현국,Aman-ur-Rehman,이재구 한국진공학회 2011 한국진공학회 학술발표회초록집 Vol.41 No.-

워크플로우 기반의 시맨틱 웹을 이용한 지식관리 시스템 프레임워크

권형철,최덕원,이동철 한국산업경영시스템학회 2004 한국산업경영시스템학회 학술대회 Vol.2004 No.춘계

A framework for knowledge management system has been explored which enables the semantic search of knowledge on the web. Knowledge representation by RDF and RDF schema enables machine cognition of knowledge documents. Dublin core was adopted for structured metadata representation. Thereby, it enables the CBR and rule based reasoning for intelligent knowledge retrieval. Grafting of the WFMS technique unto the KMS facilitates the effective utilization of process knowledge and creation of new knowledge.

Radiation Therapy (RT) of Midline Granuloma

권형철(Hyoung Cheol Kwon),오윤경(Yoon Kyeong Oh),길학준(Hak Jun Gil),윤세철(Sei Chul Yoon),박용휘(Yong Whee Bahk) 대한방사선종양학회 1986 Radiation Oncology Journal Vol.4 No.2

중앙성육아종 환자 7예에 대하여 가톨릭의대 강남성모병원 방사선치료실에서는 1983년 3월 부터 1986년 6월까지, 사이에 외부방사선치료를 시행하였다. 임상증상으로서, 모든 환자에서 침습부위에 궤사를 동반한 부비동염 증세를 보였고, 1예에선 대장(결장)병변도 나타냈다. 방사선치료는 6MV선형가속기를 사용하여 비강 및 부비동 부위를 포함하는 상기도 부위에 2~3문 조사를 실시하여, 총선량 4,000~5,000cGy/5~6주를 조사하였다. 방사선치료 반응으로서 방사선치료 종료 후 1.5~20개월 추적관찰 한 결과 완전 및 부분관해가 각각 3예, 대장부위에 병변을 보였던 1예에선 병이 진행되고 있음이 관찰되었다. Seven patients having midline granuloma received local irradiation from March 1983 to June 1986. Clinically, all of the 7 patients had pansinusitis with necrotic destruction of the involved sites and one case revealed colonic lesion. Each of the patients received a tumor dose of 4,000~5,000 cGy/5~6 wks to the upper aerodigestive tract using a 6-MV linear accelerator. Complete and partial remission occured in 3 patients each, and in one case, the disease progressed despite of the irradiation.

쥐 섬유육종에서 베타카로틴과 방사선조사 병용의 항종양 효과

권형철(Hyoung-Cheol Kwon),양문식(Moon-Sik Yang) 대한방사선종양학회 2000 Radiation Oncology Journal Vol.18 No.2

목 적 : 베타카로틴과 방사선조사의 병용효과에 관한 평가를 목적으로, 베타카로틴을 병용한 경우 방사선조사 단독의 경우 보다 세포독성의 차이는 어떠하며, 또한 쥐 섬유육종에서 두 군간의 종양성장의 지연 정도에 어떠한 차이가 있는가를 관찰하고자 본 연구를 시행하였다. 대상 및 방법 : 2% 베타카로틴 유제를 2 mg/ml 으로 만든 다음 단계적으로 희석하여 사용하였으며, 섬유육종세포와 태생 5∼6주의 C3H/N의 실험쥐를 이용하였다. 방사선조사는 6 MV 선형가속기를 이용하였고, 세포내 독성은 쥐 섬유육종세포의 생존을 감소시키는 능력으로 평가하였으며, 베타카로틴 2 mg/ml을 방사선조사 1시간 전 섬유육종세포주에 접촉시켰다. 종양성장 지연 실험을 위하여 베타카로틴과 방사선조사 병용군(n=6)과 방사선조사 단독군(n=5)으로 분류하였으며, 베타카로틴 20 mg/kg을 방사선조사 30분전 섬유육종이 접종된 쥐의 복강내 일회 주사하였고, 방사선조사량은 20 Gy를 주었다. 종양용적은 장경×장경×장경/2 (mm3) 공식을 사용하였으며, 2∼3일 마다 측정하였다. 결 과 : 섬유육종세포에 베타카로틴 0.002, 0.02, 0.2, 2 mg/ml 농도액을 1시간 동안 접촉 후 얻은 각각 생존분율은 0.69±0.07, 0.59±0.08, 0.08±0.008 및 0.02±0.006이었다. 그리고 방사선조사 1시간 전 섬유육종세포에 베타카로틴 2 mg/ml을 접촉한 후 조사량 2, 4, 6 및 8 Gy에서 얻은 각각의 생존분율은 0.13±0.05, 0.03±0.005, 0.01±0.002 및 0.009±0.0008이었으며 방사선조사 단독군의 경우 동일 조사량에서 얻은 생존분율은 각각 0.66±0.05, 0.40±0.04, 0.11±0.01 및 0.03±0.006으로 나타났다(p<0.05). 종양성장의 지연정도를 나타내는 실험에서 섬유육종을 쥐에 접종한 후 종양의 용적이 1,000 mm3 에 달하는 기간은 베타카로틴 병용군과 방사선조사 단독군에서 각각 18일과 19일로 나타났다(p>0.05). 결 론 : 쥐 섬유육종세포에 베타카로틴을 접촉한 경우 세포독성이 나타났으며, 베타카로틴 농도 증가에 따라 세포독성도 증가하였다. 그리고 쥐 섬유육종세포의 세포독성은 베타카로틴 병용군에서 방사선조사 단독군의 경우 보다 부가적으로 증가하였으며, 두 군간에 통계학적으로 현저한 차이를 보였다. 그러나 쥐 섬유육종 성장 지연정도에 있어서 베타카로틴 병용군과 방사선조사 단독군간의 통계학적으로 뚜렷한 차이는 없었다. Purpose :To investigate whether combined beta- carotene with X- irradiation has more enhanced radition response than X- irradiation or not, we performed a experiment about in vitro cytotoxicity of beta- carotene and/or X- irradiation in the fibrosarcoma cells, tumor growth delay of combined beta- caroten with/or X- irradiation in the mouse fibrosarcoma. Materials and Methods :2% emulsion of beta- carotene was serially diluted and used. X- irradiation was given by 6 MeV linear accelerator. The cytotoxicity of beta- carotene in vitro was evaluated from clonogenic assay. To compare the cytotoxicity between combined beta- carotene with X- irradiation and X- irradiation group, 2 mg/ml of beta- carotene was contacted to fibrosarcoma (FSaII) cells for 1 hour before X- irradiation. For the tumor growth delay, single 20 Gy was given to FSaII tumor bearing C3H/N mice whic was classified as beta- crotene with X- irradiation group (n=6) and X- irradiation alone group (n=5). 0.2 ml of 20 mg/kg of beta- carotene were i.p. injected to mice 30 minute before X- irradiation in the beta- crotene with X- irradiation group. The tumor growth delay defined as the time which reach to 1,000 mm3 of tumor volume. Result : (1) Cytotoxicity in vitro; 1) survival fraction at beta- carotene concentration of 0.002, 0.02, 0.2 and 2mg/ml were 0.69±0.07, 0.59±0.08, 0.08±0.008 and 0.02±0.006, respectively. 2) each survival fraction at 2, 4, 6 and 8 Gy in the 2 mg/ml of beta- carotene +X- irradiation group were 0.13±0.05, 0.03±0.005, 0.01±0.002 and 0.009±0.0008, respectively. But each survival fraction at same irradiation dose in the Xirradiation group were 0.66±0.05, 0.40±0.04, 0.11±0.01 and 0.03±0.006, respectively(p<0.05). (2) The time which reach to 1,000 mm3 of tumor volume of beta- carotene + X- irradiation group and X- irradiation alone group were 18, 19 days, respectively(p>0.05). Conclusion :The contact of beta- caroten to FSaII cells showed mild cytotoxicity which was increased according to concentration. The cytotoxicity of combined beta- carotene with X- irradiation more increased than that of X- irradiation, additionaly. And there was significant difference of cytotoxicity between two groups. But there were no significant difference of the growth delay of fibrosarcoma between two groups.