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김신애,구형진,김진서,김해은,박지민,장정호 한국과학영재교육학회 2018 과학영재교육 Vol.10 No.3
산업화가 가속화되며 대기 오염으로 인한 각종 문제들이 끊임없이 제기되고 있다. 또한 미세먼지 및 황사로 인한 문제들이 심각한 수준에 이르렀다. 대기 오염은 인간 생활에 큰 변화를 가져오고 있으며, 오염된 대기가 빗물에 섞여 내리면서 수질 오염으로도 이어질 수 있다. 우리는 대기와 수질 오염의 매개 역할을 할 수 있는 빗물의 오염 여부에 대해 연구할 필요가 있다고 생각했다. 일반적인 빗물의 오염으로 알려진 산성비에 대해서는 잘 알려져 있지만 대기 오염으로 인해 빗물의 미생물에 변화가 있는지는 잘 알려져 있지 않다. 이에 우리는 대구 및 포항 지역 4개 장소(대구 북구 복현동, 대현동, 수성구 황금동, 포항 북구 우현동)에서 빗물을 수집하여 각 빗물 샘플에서 미생물을 배양하였다. 이후 유전자증폭(PCR)을 통해 각 미생물의 16S rDNA를 획득하고, DNA 염기서열 분석(DNA sequencing)과 유사 DNA 검색(BLAST search)을 진행하여 미생물을 동정하였다. 또한 각 미생물의 서식지, 특성 및 병원성 유무 등을 조사하였다. 배양 결과 18종의 미생물이 동정되었으며, 이 중 병원성을 지니는 미생물도 8종 확인되었다. 이 연구결과는 빗물의 오염에 대한 미생물학적 정보를 제공할 수 있을 것으로 기대한다. Currently, air pollutions such as micro-dust and yellow dust have reached a critical level that negatively affect on human health in Korea. Moreover, air pollution makes a big difference in human life style, and polluted air can also lead to water pollution by merged to raindrops. We thought we needed to elucidate the contamination of rainwater that could serve as a bridge for air and water pollution. Acid rain is commonly known as the contamination of rainwater as well. However, there are little information if air pollution causes changes in the microbial life of rain. In response, we collected raindrops from four locations in Daegu and Pohang cities; Bokhyeon-dong, Daehyeon-dong, Hwanggeum-dong in Daegu, and Woohyeon-dong in Pohang. Each of the raindrop samples were incubated at 37°C for cultivating microorganisms using two types of media LB and R2A. Afterwards, 16S rDNA of each microorganism was amplified through PCR, and DNA sequencing and DNA BLAST search was performed. We also examined the habitat, characteristics, and pathogenic presence of each microorganism. Eight microorganisms were identified as pathogenic from eighteen samples. This study may provide the microbiological information about the contamination of rainwater.
미생물 농약과 유기합성 살균제 혼용에 따른 고추 흰가루병 방제 효과
홍성준(Sung-Jun Hong),김정현(Jung-Hyun Kim),김용기(Yong-Ki Kim),지형진(Hyeong-Jin Jee),심창기(Chang-Ki Shim),김민정(Min-Jeong Kim),박종호(Jong-Ho Park),한은정(Eun-Jung Han),구형진(Hyung-Jin Goo),최광영(Kwang-Young Choi) 한국농약과학회 2014 농약과학회지 Vol.18 No.4
This study was conducted to reduce the using amount of chemical fungicides for the control of red-pepper powdery mildew. Effect of combined application of three microbial fungicides and six chemical fungicides for the control of red-pepper powdery mildew was examined in vitro, in pot assay and under field condition. One chemical fungicide (Azoxystrobin+Chlorothalonil) among six chemical fungicides significantly suppressed three microbial fungicides (Bacillus subtilis Y1336, Bacillus subtilis DBB1501, Bacillus subtilis QST-713) registered for the control of pepper powdery mildew in vitro. In the pot assay, two mixed application such as B. subtilis DBB1501+Trifloxystrobin, B. subtilis QST713+Trifloxystrobin among nine mixed applications of three microbial fungicides and three chemical fungicides showed the highest suppressive effect against red pepper powdery mildew. Also, suppressive effect of the mixed application of B. subtilis QST713 and Trifloxystrobin was similar to that of single application of three chemical fungicides(Myclobutanil, Trifloxystrobin, Hexaconazole). In the field test, when the microbial fungicides (B. subtilis DBB1501, B. subtilis QST713) and the chemical fungicide (Trifloxystrobin) for the control of powdery mildew of red pepper were mixed foliar sprayed four times at 7 day-intervals, the control values were in the range of 70.3% to 70.9%. On the other hand, when each of the chemical fungicide (Trifloxystrobin) was foliar sprayed four times at 7 day-intervals, the control value was 72.7%. Consequently, the mixed application of the microbial fungicides and chemical fungicides could be recommended as a one of control measures for reducing the using amount of chemical fungicides.