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Response of Bioluminescent Bacteria to Sixteen Azo Dyes
구만복,Hwa Young Lee,Sue Hyung Choi 한국생물공학회 2003 Biotechnology and Bioprocess Engineering Vol.8 No.2
Recombinant bioluminescent bacteria were used to monitor and classify the toxicity of azo dyes. Two constitutive bioluminescent bacteria, Photobacterium phosphoreum and Escherichia coli, E. coli GC2 (lac::luxCDABE), were used to detect the cellular toxicity of the azo dyes. In addition, four stress-inducible bioluminescent E. coli; DPD2794 (recA::luxCDABE), a DNA damage sensitive strain; DPD2540 (fabA::luxCDABE), a membrane damage sensitive strain; DPD2511 (katG::luxCDABE), an oxidative damage sensitive strain; and TV1061 (grpE::luxCDABE), a protein damage sensitive strain, were used to provide information about the type of toxicity caused by crystal violet, the most toxic dye of the 16 azo dyes tested. These results suggest that azo dyes result in serious cellular toxicity in bacteria, and that toxicity monitoring and classification of some azo dyes, in the field, may be possible using these recombinant bioluminescent bacteria.
Specific Detection of DNA Using Quantum Dots and Magnetic Beads for Large Volume Samples
구만복,김연석,Byoung Chan Kim,Jin Hyung Lee,Jungbae Kim 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.5
Here we present a sensitive DNA detection protocol using quantum dots (QDs) and magnetic beads (MBs) for large volume samples. In this study, QDs, conjugated with streptavidin, were used to produce fluorescent signals while magnetic beads (MBs) were used to isolate and concentrate the signals. The presence of target DNAs leads to the sandwich hybridization between the functionalized QDs, the target DNAs and the MBs. In fact, the QDs-MBs complex, which is bound using the target DNA, can be isolated and then concentrated. The binding of the QDs to the surface of the MBs was confirmed by confocal microscopy and Cd elemental analysis. It was found that the fluorescent intensity was proportional to concentration of the target DNA, while the presence of non-complementary DNA produced no significant fluorescent signal. In addition, the presence of low copies of target DNAs such as 0.5 pM in large volume samples up to 40 mL was successfully detected by using a magnet-assisted concentration protocol which consequently results in the enhancement of the sensitivity more than 100-fold.
Characterization of gltA::luxCDABE Fusion in Escherichia coli as a Toxicity Biosensor
구만복,안주명,Byoung Chan Kim 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.6
The use of gltA gene, as a new biomarker for environmental stress biomonitoring, was investigated because of its key position as the first enzyme of the tricarboxylic acid (TCA) cycle. A recombinant bioluminescent Escherichia coli strain, EBJM2, was constructed using a plasmid carrying the citrate synthase (gltA) promoter transcribing the Photorhabdus luminescens luxCDABE genes (gltA::luxCDABE). The responses from this strain were studied with five different classes of toxicants: DNA damage chemicals, phenolics, oxidative-stress chemicals, PAHs, and organic solvents. EBJM2 responded strongly to DNA damage chemicals, such as mitomycin C (MMC) and methyl-nitro-nitrosoguanidine (MNNG) and nalidixic acid with the strongest responses. In contrast, tests with several compounds from the other four classes of toxicants gave no significant response. Therefore, EBJM2 was found to be sensitive to DNA damage chemicals.
요양시설 사회복지사의 Care-Management 직무 전문능력에 관한 연구
조만복 ( Man Bok Cho ),박용억 ( Yong Euk Park ),박미주 ( Mi Joo Park ) 한국노인의료복지학회 2010 노인의료복지연구 Vol.1 No.3
This study is purposed to closely examine the professional competence needed for specializing care-management tasks by social workers at nursing facilities and to develop measures for professional competence under the conditions that specialized social workers are needed in order to improve management and the quality of service at nursing facilities of the aged and the severely disabled in the country. Therefore, this study attempted to first develop measures for social workers at nursing facilities in the country to achieve professional competence for care-management tasks, and secondly to apply the developed measures on social workers, and to find out the situations of their professional competence and to verify the distinctive measures. In order to develop the measures, three steps were taken. The first stage is to identify professional competence that are needed to specialize care-management tasks, and the second stage is to map out measures for professional competence, and the third stage is to develop measures by consulting with experts. The group of experts had confirmed the analyzed results of actual inspection through documentary study and focused group, and revised 18 items for essential measures of professional competence for care-management tasks after thorough discussions. And in order to check the validity of contents by the experts, each item was reviewed with 4 or 5 points, calculated into the ratio as CVR (Content Validity Ratio), and selected items with CVR 0.8 or more. By analyzing the data, 8 regular items with CRV 0.8 or more, and 40 items of index were selected as well. The five experts lastly developed 40 lists in 8 areas, basic knowledge of care-management, initial counseling, assessments, writing care-plan, service management, service evaluation, education and training, and other managements, through discussions and agreements.