[P3-78] 상백피와 강황 혼합 추출물 첨가 국수의 저장성 및 품질 증진 효과

곽지희,김꽃봉우리,이소영,송유진,윤소영,이소정,이청조,박나비,정지연,안동현,이호동 한국식품영양과학회 2009 한국식품영양과학회 학술대회발표집 Vol.2009 No.11

[P3-39] 물리적 처리에 의한 중력분 반죽 gliadin의 항원성 변화

곽지희,김꽃봉우리,송유진,이청조,김민지,정지연,최문경,김동현,선우찬,박진규,김재훈,이주운,변명우,안동현 한국식품영양과학회 2010 한국식품영양과학회 학술대회발표집 Vol.2010 No.10

Changes in Coronary Perfusion after Occlusion of Coronary Arteries in Kawasaki Disease

곽지희,송진영,강이석,허준,이흥재 연세대학교의과대학 2014 Yonsei medical journal Vol.55 No.2

Purpose: Myocardial infarction in children with total occlusion of a coronary arteryafter Kawasaki disease is rare due to multiple collateral vessels. We aimed to investigate the changes in coronary perfusion associated with coronary artery occlusionafter Kawasaki disease. Materials and Methods: Eleven patients with coronaryartery occlusion after Kawasaki disease were investigated. Serial coronary angiographiesafter total occlusion of a coronary artery were reviewed and the changes were described in all patients with additive information collected. Results: The median age at the occlusion was 5.9 years old. The interval to occlusion was 6.2±6.9 years. Four left anterior descending coronary artery total occlusions and 10 right coronary artery total occlusions were detected. Immediate coronary artery bypassgraft for left anterior descending coronary artery total occlusion made right coronary total occlusion occurred in all except one patient and the intervals thereof were 1 year, 1.8 years, and 4 years. Collaterals to the left coronary artery regressed after recanalization, while new collaterals to the right coronary artery developed. In three, collaterals to the right coronary artery decreased without recanalization withoutclinical signs. Conclusion: The right coronary artery should be followed up carefully because of possible occlusion of new onset or changes in collaterals.

Impulse Oscillation System의 단기 변동성

곽지희 ( Ji Hee Kwak ),황필주 ( Pil Joo Hwang ),정혜영 ( Hye Young Jung ),정다운 ( Da Woon Jung ),한만용 ( Man Yong Han ) 대한천식알레르기학회 2008 천식 및 알레르기 Vol.28 No.4

Background: The impulse oscillation system (IOS) is known to be more useful than spirometry for the evaluation of pulmonary function. Objective: The aim of this study was to compare short-term variations in spirometry and IOS parameters in healthy adults. Method: Spirometry and IOS parameters were measured in 12 healthy adults. Their mean age was 28.7±3.64 years; there were 6 males and 6 females. The differences in the mean values at various time intervals were tested with repeated-measures analysis of variance (ANOVA). The coefficients of repeatability and variation were calculated for assessing the repeatability and variability. Result: There were no statistically significant differences in the mean values of the spirometric parameters, FEV1, FVC, FEV1/FVC, FEV0.5, and PEF, and the IOS parameters, X5 and R20. In spirometry, the coefficient of repeatability on the first day was the smallest for FVC, FEV1/FVC, and MMEF. However, when IOS was used, it was the smallest only for Rf. The coefficient of variation in spirometry was the smallest for FEV1/FVC, and that in IOS was the smallest for R5 and X5. Conclusion: The variation in IOS values is larger than that in most of the spirometry parameters. X5 is the smallest coefficient of variations and statistically significant among the IOS parameters. (Korean J Asthma Allergy Clin Immunol 2008;28:271-276)

멸치 염용성 단백질 추출물 첨가가 소시지의 품질에 미치는 영향

곽지희(Ji-Hee Kwak),김꽃봉우리(Koth-Bong-Woo-Ri Kim),송유진(Eu-Jin Song),이청조(Chung-Jo Lee),정지연(Ji-Yeon Jung),최문경(Moon-Kyoung Choi),김민지(Min-Ji Kim),안동현(Dong-Hyun Ahn) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.12

멸치의 염용성 단백질 추출물을 5 및 10% 농도로 첨가하여 제조한 소시지의 저장성, 품질 및 관능적 특성을 조사하였다. 소시지를 제조 후 10℃에서 35일간 저장하면서 저장성을 알아본 결과, 생균수 및 VBN 함량은 첨가구와 무첨가구 사이에 큰 차이를 보이지 않아 추출물이 소시지의 품질에 영향을 주지 않았다. 또한 멸치의 염용성 단백질 추출물이 소시지의 품질에 미치는 영향을 알아보기 위해 유화안정성, pH, 수분함량, 색도 및 물성을 측정하고 관능평가를 실시하였다. 그 결과, 유화안정성, pH, 수분함량 및 색도는 저장기간 동안 첨가구와 무첨가구가 유사한 값을 나타내었다. 물성측정 결과, 경도는 염용성 단백질 추출물 첨가 농도가 높아짐에 따라 유의적으로 증가하였으며, 검성은 5% 첨가구가 가장 높은 값을 나타내었다. 반면 10% 첨가구는 검성, 씹힘성, 응집성 및 복원성에서 유의적으로 가장 낮은 값을 나타내었다. 관능평가 결과에서는 5% 첨가구가 외관의 형태 및 탄력성 항목에서 유의적으로 가장 높은 평가를 받았다. 이상의 결과를 종합해볼 때, 소시지에 멸치 염용성 단백질 추출물을 5% 정도 첨가할 경우 무첨가구와 비교 시 조직감이 향상되고 관능적 선호도에서 탄력성이 좋은 평가를 받아 소시지의 결착력이 증진됨을 알 수 있었다. 따라서 멸치 염용성 단백질을 소시지에 첨가 시 품질향상과 더불어 단순한 가공 형태가 아닌 새로운 멸치 가공방법으로 차별성이 있으며, 저급 멸치의 사용으로 가격 경쟁력도 가지게 되어 제한된 멸치의 이용을 확대시킬 수 있을 것으로 기대된다. This study was conducted to evaluate the effects of salt soluble protein extracts from anchovy (ASSPE) on sausage during storage at 10oC. Sausages were produced containing 5 and 10% ASSPE. Sausages containing ASSPE showed no significant differences in emulsion stability, color, pH, moisture, viable cell count, and VBN compared to the control. The hardness significantly increased with the addition of ASSPE, and sausages containing 5% ASSPE were the highest in gumminess. In the sensory evaluation, sausages containing 5% ASSPE had the highest score in appearance and springiness. In conclusion, the addition of ASSPE in sausage had no effects on storage characteristics, but had a good effect on improvement of quality and sensory characteristics. Especially, 5% ASSPE sausage had good texture and high sensory characteristics due to increased binding capacity. These results suggest that 5% ASSPE may improve the quality and sensory characteristics of sausage.

가압가열 및 microwave에 의한 중력분 반죽 gliadin의 항원성 변화

곽지희(Ji-Hee Kwak),김꽃봉우리(Koth-Bong-Woo-Ri Kim),이청조(Chung-Jo Lee),김민지(Min-Ji Kim),김동현(Dong-Hyun Kim),선우찬(Chan Sunwoo),정슬아(Seul-A Jung),김현지(Hyun-Jee Kim),최정수(Jung-Su Choi),김성원(Seong-Won Kim),안동현(Dong-Hyun 한국식품과학회 2012 한국식품과학회지 Vol.44 No.1

본 연구에서는 가압가열 및 microwave 처리에 의한 중력분 반죽 추출물 내의 gliadin 단백질의 항원성 변화에 대해 살펴보았다. 중력분 반죽에 가압가열과 microwave를 단독 또는 병행으로 처리하여 ci-ELISA, SDS-PAGE 및 immunoblotting을 실시하였으며, 가압가열 처리에 의해서 anti-gliadin IgG 항체와 gliadin과의 결합력이 다소 감소한 것을 확인하였다. 특히 30 min 이상 처리시 더욱 감소한 것으로 나타났으며, SDS-PAGE와 immunoblotting 결과에서도 gliadin band의 강도가 약해지고 항체와의 반응도 나타나지 않았다. Microwave 처리의 경우, 5 min 이상 처리시 일부 gliadin 단백질의 소실이 관찰되었으나, 항원성에는 큰 변화가 없었다. 또한 가압가열 및 microwave 병행 처리에 의해 항원-항체 결합력이 더욱 감소되었으며, 특히 가압가열 50 min, microwave 10 min 처리시 약 35.0%로 감소되었다. 이상의 결과를 통해 가압가열 처리에 의해 중력분 반죽 추출물 내 gliadin의 항원성이 감소되는 것을 확인하였으며, microwave와 병행 처리하는 경우, 더욱 감소하는 것을 확인하였다. The aim of this study was to determine the optimal physical treatment to reduce the antigenicity of gliadin in wheat dough. Medium wheat dough was treated with an autoclave (5, 10, 30, and 50 min at 121℃, 1 atm), a microwave (1, 5, and 10 min) or both (10, 30, and 50 min/5, 10 min). The proteins in the dough extracts were analyzed by SDS-PAGE and the binding ability of anti-gliadin IgG to gliadin was examined by ci-ELISA and immunoblotting. Results showed that the ability of anti-gliadin IgG to bind to gliadin in wheat dough treated with an autoclave alone or in combination with a microwave was decreased. Especially, it declined to ~77% after autoclaving for 30 min and 35% after both autoclaving for 50 min and microwaving for 5 min. In addition, the intensity of gliadin bands in SDS-PAGE were weakened and anti-gliadin IgG did not recognize gliadin in immunoblotting. However, microwaving alone did not affect the antigenicity of gliadin in wheat dough. These results indicate that autoclaving may affect the reduction of the antigenicity of gliadin in medium wheat dough. Moreover, autoclaving in combination with microwaving is more effective for reducing the antigenicity of wheat dough.

가압가열 및 Microwave 처리에 의한 중력분 Gliadin의 항원성 변화

곽지희(Ji-Hee Kwak),김꽃봉우리(Koth-Bong-Woo-Ri Kim),이청조(Chung-Jo Lee),김민지(Min-Ji Kim),김동현(Dong-Hyun Kim),선우찬(Chan Sunwoo),정슬아(Seul-A Jung),강주연(Ju-Youn Kang),김현지(Hyun-Jee Kim),최정수(Jung-Su Choi),김성원(Seong-Won 한국식품영양과학회 2011 한국식품영양과학회지 Vol.40 No.10

본 연구에서는 가압가열 및 microwave 처리가 gliadin의 항원성에 미치는 영향을 살펴보기 위해 중력분에 가압가열과 microwave를 단독 또는 병행으로 처리하여 Ci-ELISA, SDS-PAGE 및 immunoblotting을 실시하였다. 가압가열 처리의 경우, 처리 시간이 길어질수록 IgG와의 결합력이 감소하였으며, 특히 50분 처리구에서 약 69%로 가장 낮은 결합력을 보였다. 또한 SDS-PAGE와 immunoblotting 결과에서도 무처리구에서 강하게 보였던 gliadin band가 처리에 의해 거의 소실되고 항체와 반응하지 않았다. 가압가열 및 microwave를 병행 처리한 경우도 마찬가지로 gliadin의 결합력이 다소 감소하였으며, 처리구 중에서는 가압가열 50분, microwave 5분 처리구에서 약 73%로 가장 낮은 결합력을 보였다. 반면 microwave를 단독으로 처리하였을 때에는 일부 단백질의 변화는 관찰되었으나 항원성 감소에는 큰 영향을 미치지 않았다. 이상의 결과를 통해 가압가열을 단독 처리에 의해 gliadin의 항원성이 다소 감소되었으며, microwave 병행 처리에 의한 차이는 크게 나타나지 않은 것을 확인하였다. This study was conducted to evaluate the effect of physical treatments on the antigenicity of gliadin in medium wheat flour. The wheat flour was treated with an autoclave (5, 10, 30, 50 min), a microwave (1, 5, 10 min), and both (10, 30, 50 min/ 5, 10 min), and investigated by SDS-PAGE, immunoblotting and Ci-ELISA using anti-gliadin IgG. The results showed that the binding ability of anti-gliadin IgG to gliadin in wheat flour was slightly decreased when autoclaved or when autoclaved and microwaved. Especially, it was reduced to about 69% after autoclaving for 50 min and 73% after autoclaving for 50 min and microwaving for 5 min. In addition, gliadin bands in the 50 min autoclaved group disappeared in both SDS-PAGE and immunoblotting. On the other hand, the antigenicity of gliadin was unaffected by microwaving alone. Consequently, there were no considerable changes in using an autoclave alone or in combination with a microwave. These results suggest that autoclaving may affect the reduction of the antigenicity of gliadin in medium wheat flour.

소아의 기도 폐쇄 평가에서 impulse oscillometry system과 폐활량 측정법의 비교

허혜영,곽지희,김형윤,정다운,신윤호,한만용 대한소아청소년과학회 2008 Clinical and Experimental Pediatrics (CEP) Vol.51 No.8

Purpose:Measurement of forced expiratory volume in 1 second (FEV1) is usually difficult to obtain in children under six years of age because it requires active cooperation. This study evaluates the sensitivity of impulse oscillometry system (IOS) parameters for detecting airway obstruction in comparison with FEV1. Methods:We studied 174 children who performed the lung function and methacholine challenge tests to diagnose asthma by IOS and spirometry. Children were divided into two subgroups according to their PC20, which is a parameter for bronchial sensitivity. We compared IOS parameters with FEV1 at the baseline, post-methacholine challenge, and evaluated their correlation. Results:At the baseline, reactance at 5 Hz (X5) and resistance at 5 Hz (R5) significantly differed between the PC20 positive (PC20≤16 mg/mL) group and PC20 negative (PC20 >16 mg/mL) group; however, FEV1, FEV1 % predicted, FEV1_Zs (Z score) did not differ. FEV1 is correlated with X5 (r=0.45, P<0.01) and R5 (r=―0.69, P<0.01). FEV1_Zs is also correlated with X5_Zs (r=―0.26, P<0.01) and R5_Zs (r=―0.31, P<0.01). After the methacholine challenge test, dose-response slopes in FEV1 and X5 significantly differed between the two subgroups (P<0.05). Conclusion:IOS parameters were more discriminative than FEV1 for detecting decreased baseline lung function between two subgroups and have a good correlation with FEV1. (Korean J Pediatr 2008;51:842-847) Purpose:Measurement of forced expiratory volume in 1 second (FEV1) is usually difficult to obtain in children under six years of age because it requires active cooperation. This study evaluates the sensitivity of impulse oscillometry system (IOS) parameters for detecting airway obstruction in comparison with FEV1. Methods:We studied 174 children who performed the lung function and methacholine challenge tests to diagnose asthma by IOS and spirometry. Children were divided into two subgroups according to their PC20, which is a parameter for bronchial sensitivity. We compared IOS parameters with FEV1 at the baseline, post-methacholine challenge, and evaluated their correlation. Results:At the baseline, reactance at 5 Hz (X5) and resistance at 5 Hz (R5) significantly differed between the PC20 positive (PC20≤16 mg/mL) group and PC20 negative (PC20 >16 mg/mL) group; however, FEV1, FEV1 % predicted, FEV1_Zs (Z score) did not differ. FEV1 is correlated with X5 (r=0.45, P<0.01) and R5 (r=―0.69, P<0.01). FEV1_Zs is also correlated with X5_Zs (r=―0.26, P<0.01) and R5_Zs (r=―0.31, P<0.01). After the methacholine challenge test, dose-response slopes in FEV1 and X5 significantly differed between the two subgroups (P<0.05). Conclusion:IOS parameters were more discriminative than FEV1 for detecting decreased baseline lung function between two subgroups and have a good correlation with FEV1. (Korean J Pediatr 2008;51:842-847)

물리적 처리에 의한 중력분 및 중력분 반죽 gliadin의 항원성 및 이화학적 특성 변화

최미라,곽지희,김꽃봉우리,이청조,김민지,김동현,선우찬,정슬아,김현지,정다현,최정수,안동현 한국식품영양과학회 2011 한국식품영양과학회 학술대회발표집 Vol.2011 No.10

집먼지 진드기 항원을 이용한 초급속면역치료의 안전성과 효과

현세은,김형윤,곽지희,신윤호,서지영,한만용 대한소아청소년과학회 2008 Clinical and Experimental Pediatrics (CEP) Vol.51 No.8

Purpose:Immunotherapy is accepted as the only treatment of allergic disease that can modify the natural course of the disease and ameliorate symptoms. This study aimed to evaluate the safety and efficacy of ultra-rush therapy using Dermatophagoides extracts in children. Methods:Of children older than four years who had visited Bundang CHA Pediatric Allergy Clinic, those showing positive reactions only to Dermatophagoides in the skin prick test and to the nasal provocation test were included. In all, 11 and 12 patients respectively preferred conventional and ultra-rush immunotherapy. We elevated allergen concentrations diluted to 1,000:1 of the end strength by 2-3 times with 30-minute intervals and checked oxygen saturation, pulse rate, blood pressure, and systemic reactions every 15 minutes. Immunotherapy effectiveness was valued by changes in nasal provocation test scores before and after immunotherapy. Results:The average ages of patients in the conventional and ultra-rush immunotherapy groups were 8.3±2.3 and 9.2±2.8 years, respectively. Systemic reactions were observed in six in the ultra-rush group (50%) without anaphylaxis and one (9%) in the conventional group. The average scores in the nasal provocation test before and after treatment in the conventional group were 8.2±1.5 and 4.6±2.1, respectively (P=0.043). In the ultra-rush immunotherapy group, the scores changed from 6.2±2.2 to 3.7±2.5 (P=0.017). Conclusion:Ultra-rush immunotherapy using Dermatophagoides in children is effective for treating allergic disease but can induce systemic effects rather than conventional immunotherapy. (Korean J Pediatr 2008;51:868-873) Purpose:Immunotherapy is accepted as the only treatment of allergic disease that can modify the natural course of the disease and ameliorate symptoms. This study aimed to evaluate the safety and efficacy of ultra-rush therapy using Dermatophagoides extracts in children. Methods:Of children older than four years who had visited Bundang CHA Pediatric Allergy Clinic, those showing positive reactions only to Dermatophagoides in the skin prick test and to the nasal provocation test were included. In all, 11 and 12 patients respectively preferred conventional and ultra-rush immunotherapy. We elevated allergen concentrations diluted to 1,000:1 of the end strength by 2-3 times with 30-minute intervals and checked oxygen saturation, pulse rate, blood pressure, and systemic reactions every 15 minutes. Immunotherapy effectiveness was valued by changes in nasal provocation test scores before and after immunotherapy. Results:The average ages of patients in the conventional and ultra-rush immunotherapy groups were 8.3±2.3 and 9.2±2.8 years, respectively. Systemic reactions were observed in six in the ultra-rush group (50%) without anaphylaxis and one (9%) in the conventional group. The average scores in the nasal provocation test before and after treatment in the conventional group were 8.2±1.5 and 4.6±2.1, respectively (P=0.043). In the ultra-rush immunotherapy group, the scores changed from 6.2±2.2 to 3.7±2.5 (P=0.017). Conclusion:Ultra-rush immunotherapy using Dermatophagoides in children is effective for treating allergic disease but can induce systemic effects rather than conventional immunotherapy. (Korean J Pediatr 2008;51:868-873)