검정하우스를 이용한 벼 바이러스병 저항성 대량 검정체계

곽도연,오병근,김순철,양세준 한국육종학회 2003 한국육종학회 학술발표회 발표요지 Vol.35 No.-

온실을 이용한 벼 바이러스병 저항성 대량검정

곽도연,김순철,양세준,여운상,박노봉,김명기,이봉춘 한국육종학회 2002 한국육종학회 학술발표회 발표요지 Vol.34 No.2

신광벼 유래의 벼 줄무늬잎마름병 저항성 주동 QTL qSTV11SG탐색

곽도연,이봉춘,최일룡,여운상,조준현,이지윤,송유천,윤영남,박동수,강항원,남민희 한국육종학회 2011 한국육종학회지 Vol.43 No.5

벼 줄무늬잎마름병 저항성 유전자 및 연관 DNA 마커 탐색을 위하여 줄무늬잎마름병에 저항성인 통일형 품종인 신광 벼 이용 여교잡 집단을 육성하였다. 줄무늬잎마름병 저항성 유전자에 대한 QTL을 분석한 결과 11번 염색체에 위치하는 SSR 마커 RM6897이 탐색되었으며 전체 표현형 변이의 44.2%를 설명하였다. DNA 마커 RM6897은 여교잡 집단에서 생물검정과 유전자형이 일치하였다. 또한 자포니카 품종들에서 저항성 27품종과 감수성 23품종에 대해 Virus diseases often cause serious damage to rice production in Asia. The lack of information on virus resistance genes has been a major obstacle for the breeding of resistant varieties. In order to identify DNA marker associated with resistance against rice stripe virus (RSV), the quantitative trait locus (QTL) was carried out using advanced backcross population developed from a cross between RSV-resistant tongil type cultivar Shinkwang and susceptible japonica cultivar Ilpum. A RSV resistance QTL qSTV11SG explaining 44.2% of the phenotypic variation was identified on chromosome 11 of Tongil type rice cultivar 'Shingwang'. qSTV11SG was tightly linked to DNA marker RM6897. The RM6897 divided as resistance type allele and susceptible type alleles. Twenty seven resistant varieties showed the resistant-type allele and 23 susceptible varieties were susceptible-type allele to the marker of RM6897. This results and the molecular markers presented here may be useful in rice breeding for improving RSV resistance in japonica rice.

남부지방의 벼 줄무늬잎마름병 발생실태 및 품종 저항성

곽도연,이종희,김순철,양세준,오병근,여운상,박노봉,이봉춘 한국육종학회 2003 한국육종학회 학술발표회 발표요지 Vol.35 No.3

망실을 이용한 벼 바이러스병 저항성 대량 검정체계

곽도연,여운상,이종희,오병근,신문식,구연충 한국작물학회 2007 Korean journal of crop science Vol.52 No.2

벼 바이러스병에 대한 최선의 방제책은 저항성 품종의 육성이라 할 수 있다. 저항성 품종의 육성을 위해서는 정밀하고 대량으로 검정 할 수 있는 검정법의 확립이 무엇보다 중요하므로 이를 위해 격리 검정망실을 건립 보독충을 방사한 후 이를 계대유지 하여 검정에 이용하였다. 망실내에 바이러스 보독충율 변이에서 연중 높은 보독충율을 유지하였으며, 매개충의 밀도도 검정에 충분하게 유지되었다. 망실을 이용한 바이러스병 저항성검정의 효율성은 줄무늬잎마름병은 92~100% , 오갈병은 100%의 검정효율을 보였으며, 이러한 대량검정법은 실내유묘검정과 고도의 정의 상관을 나타내어 포장검정의 대량검정과 실내유묘검정의 정밀도 등 장점을 겸비한 유용한 방법으로 확인되었다. To breed virus resistant rice variety, developing an efficient screening method is the most important. Two screening methods such as field screening and tray screening method have been used, but the efficiency of the field screening method is too low because of environment factors and the that of the tray screening method is good but screening capability is limited with only 200~300 lines per year. To overcome those problems, mass screening method using screen house was developed. Barely as host plant of vector insect was grown in screen house in winter season. Then viruliferous insects are spread in the first spring of the initiation year and sustain them annually. Screening of virus resistance was tested two times in a year, the first screening was from April to June and the second from July to September. The virus infected rate of each susceptible varieties was increased to 92% for RSV and 100% for RDV from the second year. Also, this method can evaluate as many as 1,500~2,000 pedigree lines in one time compared with the tray screening method. The result indicates that the mass screening method using screen house, which combines the advantages of the field and tray screening methods, is proven to be more efficient and reliable.

고등학생 대상 메타버스를 활용한 예술 교과 연계 음악 감상수업지도안 개발 및 검증 연구

곽도연,정주연 이화여자대학교 교과교육연구소 2022 교과교육학연구 Vol.26 No.5

The purpose of this study was to develop and verify high school music appreciation class plans to confirm how to effectively develop art-linked music appreciation classes using metaverse including Gather Town and Cospaces Edu. For this purpose, a total of 8 music class sessions related to art, literature, and theater were organized for music pieces from Russian nationalist school, and some of the class plans were applied and tested for learners’ learning motivation and class satisfaction, and the class reviews were collected and analyzed. For the class plans that were not applied in the field, a field suitability verification was conducted for in-service teachers. Sessions 1 to 5 were art-linked music appreciation classes for 10th graders in the first year of high school in Daejeon Metropolitan City, and Sessions 1 to 2 did not use metaverse while Sessions 3 to 5 used metaverse to compare the students′responses. The study results showed that the class using metaverse had a positive effect on the students’ learning motivation, class satisfaction and experience in art-linked appreciation. However, along with the advantages, the disadvantages of device use were also identified. Sessions 6 to 8 were related to literature and theater, and they were revised and supplemented based on the results of field suitability verification. It was found that the art-linked music appreciation class using metaverse had a positive effect on learners, but in terms of the operation of platforms or students considering the metaverse activities as gaming, the pros and cons of metaverse-based classes were identified. It is suggested that various follow-up studies on classes using metaverse are needed responding to the changing educational paradigm. 본 연구는 메타버스를 활용한 예술 연계 음악 감상 수업의 효과적 구성 방안을 제안하기 위하여 고등학교 감상 수업지도안 개발과 검증에 목적을 두고 연구를 진행하였다. 이에 따라 민족주의 악곡을 제재곡으로 미술, 문학, 연극과 연계된총 8차시의 음악 감상 지도안을 구성하였으며 개발한 지도안 일부를 실제 적용 및 학습자의 학습동기와 수업만족도 검사를시행하고, 학생과 교사의 수업 후기를 수집, 분석하였다. 실제 적용하지 않은 지도안은 현직 교사의 현장 적합도 검증을 시행하였다. 개발한 8차시의 지도안 중 1~5차시는 미술 연계 음악 감상 수업으로, 대전광역시에 있는 고등학교 1학년 8학급을대상으로 1~2차시는 메타버스를 활용하지 않고, 3~5차시는 메타버스를 활용하여 실제 수업에 적용하고 학생들의 반응을 비교하였다. 그 결과 고등학교 음악 감상 수업에서 메타버스 활용한 수업은 학습자의 학습동기와 수업만족도 및 미술 교과 연계 감상 경험에 긍정적인 영향을 미침을 볼 수 있었지만, 장점들과 더불어 기기 활용에 따른 단점들을 살펴볼 수 있었다. 6~8 차시는 문학 및 연극과 연계한 음악 감상 지도안으로, 현장 적합도 검증 의견을 바탕으로 활동 내용 및 차시 사이 과제 등을 수정 및 보완하였다. 본 연구를 통해 개발한 메타버스를 활용한 예술 교과 연계 음악 감상 수업은 학습자에게 긍정적인영향을 미침을 볼 수 있었으나 플랫폼 활용에 따른 조작 방법이나 수업을 게임으로 여김으로써 다가오는 아쉬운 점들로부터 메타버스를 활용한 수업의 순기능과 역기능을 볼 수 있었다. 변화하는 교육 패러다임에 맞추어 메타버스를 활용한 수업에 대한 다양한 후속 수업연구가 필요함을 제언한다.

<요한시집>에서의 경계 양상과 인물간의 관계 연구

곽도연(Do Yeon Kwak) 한국현대소설학회 2014 현대소설연구 Vol.- No.56

This study was focused on analyzing The Anthology of John, one of Yong-hak Jang`s major works, and through the analysis it purposed to explain the world implemented in the novel and the author`s consciousness connoted by the story. First of all, we need to examine the general background of the novel and the examination should include ``the border`` appearing occasionally in the work. Particularly considering that Nuhye`s suicide was committed in the border, the meaning of the border must be clarified. Only then, we can see the world implemented in the novel and, going further, the reality perceived by the writer. The border is a starting point from which the world is created but, on the other hand, it is described as a place for escaping from the reality restraining the character, namely, a place where freedom is achieved. That is, the border has a double face. The border is a place of freedom that cannot be won in the reality and a place totally distinguished from the reality. Accordingly, it gives something indescribable to those who were made to live in the reality (this becomes the symbol of trauma ex post facto). For this reason, this nature of the border becomes a motive for creating the reality as a buffer zone against traumas. Like the border, Nuhye`s attitude toward Dongho has also a double face. This context is helpful to understanding Dongho`s split ego outside the concentration camp. Next, this study examined the relation between Dongho and Nuhye, the main characters of this novel. In his essay The Reality and the Anthology of John, Yong-hak Jang mentioned that the relation between the two is comparable to ``the relation between John and Jesus.`` That is, Nuhye is destined to die after paving a road for Dongho. The author added that the look of Nuhye and John is similar to freedom. Freedom also requires death for something that follows after. Let`s go back to what was mentioned earlier, namely, that Nuhye is similar to the border. Like the border creates the reality, Nuhye creates Dongho. That is, Nuhye, becoming a criterion for evaluating Dongho`s deeds, plays the role of a pivot that binds split Dongho. Furthermore, given the fact that Nuhye is a past figure existing only in Dongho`s memory, it is possible that the look of Nuhye is only Dongho`s imagination. This possibility again leads to the possibility that Dongho called in Nuhye purposely. How can we prove the possibility that Dongho called in Nuhye? Here the object ``eye`` may provide a clue to the answer. Nuhye`s eye as a body part and an illusory image restrains Dongho`s behavior powerfully. This is directly linked to Dongho`s guilty feeling, which Dongho did not arouse actively but he was given passively. That is, Dongho did not have an opportunity to commit a sin and therefore there is no reason for him to have a guilty feeling. From the beginning, Dongho was not given any chance for a sinful act. Nevertheless, Dongho assumed the burden of guilty feeling by himself, and by doing so, paradoxically he called in Nuhye`s eye. In this way, he put on Nuhye`s ``silk dress.`` As a result, what followed the death of freedom Nuhye was restraint forbidding splitting or (re)incorporation into the reality. Desire for (re)incorporation into the reality runs contrary to the proposition in the novel, namely, ``One should escape from the eye (the reality) in order to attain true freedom.`` In fact, it is impossible for humans to get out of the reality. If one escapes from the reality, though impossible, he will encounter perplexity and instinctively return to the reality. Isn`t what the author, who failed to adjust himself to the Republic of Korea in the 1950s, desired in his heart, rather incorporation into the reality than escape from that?

『무너앉는 소리』 3부작의 창작동기 연구

곽도연(Gwak, Do-yeon) 우리말글학회 2016 우리말 글 Vol.71 No.-

이 논문은 「닳아지는 살들」의 후속작들 발표 연유를 밝히는 것에 목적을 두고 있다. 그리고 「닳아지는 살들」에서 미비하게 드러냈던 부분을 보충하기 위해 후속작들이 창작되었다고 판단한다. 이를 밝혀내기 위해 먼저 「닳아지는 살들」 속 ‘꽝 당 꽝 당’과 예술성에 관해 고찰하였다. ‘꽝 당꽝 당’은 집을 무너뜨리는 소리로 드러나며 이는 가족이 해체될 것이라는 영희의 불안과 맞닿게 된다. 여기에 「닳아지는 살들」의 ‘방자한’ 예술성이 있다. 「무너앉는 소리」, 「마지막 향연」에서는 「닳아지는 살들」 속 가족의 해체양상을 반복적으로 보여준다. 그리고 반복되는 이산가족의 해산모습은 「닳아지는 살들」 속에서 나열된 역사적 사건보다 더 강력한 역사성을 담지하게 된다. ‘꽝 당 꽝 당’ 소리의 변모는 영희의 불안을 실현을 표현한다. 그리고 이산가족의 해산이라는 부분이 더욱 강조된다. 따라서 ‘꽝 당꽝 당’에서 다른 소리로의 이행은 예술성에서 역사성으로의 이행을 표식한다. 두 작품을 통해 역사성을 드러내는 이유는 작가의 정체성과 연관된다. 월남민인 작가는 남한사회의 현실을 통찰하려 하는데 이러한 의도가 두 작품의 창작을 추동하였다. 한편 작가는 미적인 것에 대한 거부를 드러낸다. 이는 예술성을 다루는 작가의 태도와도 연관된다. 즉 ‘소설가’가 다스리려는 ‘예술가’는 이호철이 다스리려는 증상이다. 그리고 닳아지는 살들의 예술성을 대변하는 ‘꽝 당 꽝 당’ 역시 작가가 해석해야 하는 것이다. 따라서 두 작품은 ‘꽝 당 꽝 당’을 해석하고 소설가의 질서로 편입하려는 시도이다. 따라서 후속작들의 창작은 작가의 정체성과 증상으로 인해 추동되었다. This paper aims to discover the reason why Lee Ho-cheol made the two sequels to Devil Sprits that Are Being Worn out. In this paper, I argue that the sequels were created to complement the deficiency of Devil Sprits that Are Being Worn out. It is a lack of critical minds about reality. To prove this, I focus the expression of “Wham boom Wham boom” and the artistry in Devil Sprits that Are Being Worn out. “Wham boom Wham boom” is a sound, which is interpreted into a sign of the collapsed family by Young-hee. This sound resonates with her anxiety about family breakdown. This narrative device give prominence to the “arrogant” artistry of Devil Sprits that Are Being Worn out. The writer shows again the appearances of family dissolution, which is described in Devil Sprits that Are Being Worn out, in the sequels, Collapsing Sound and The Last Feast. The appearances of family collapse, which is described in the sequels, give stronger historicality to the sequels than Devil Sprits that Are Being Worn out. “Wham boom Wham boom” disappears in the two sequels. It is substituted with various realistic sounds. In the sequels, where the fictional noise does not sound, the family breakdown is not Young-hee’s anxiety any more. It is real. They put more emphasis on the collapsed family. Therefore, the transition from the fictional sound to the various realistic sounds is a sign that the author’s mind trans from artistry to historicality. The transition from artistry to historicality is related to Lee Ho-cheol’s own identity. He defected to South Korea from North Korea. He intended to criticize a social reality in South Korea. The intention drove him to create the two sequels. And he reveals the refusal of aesthetic values in them. This is concerned with his attitude to artistry. Namely, “artist” whom “novelist” attempts to regulate is a symptom that he tries to regulate. “Wham boom Wham boom” that represents the artistry of Devil Sprits that Are Being Worn out is an object which he has to be construed. The two sequels are attempts to construe “Wham boom Wham boom” and regulate it into his order. Consequently, I conclude that the two sequels are the outcomes that are driven and created by his identity and the symptom.

벼 분자육종을 위한 CTAB DNA 추출 시스템 개량

이종희,곽도연,여운상,김춘송,전명기,강종래,박동수,신문식,오병근,황흥구 한국육종학회 2008 한국육종학회지 Vol.40 No.3

Many important traits have been tagged allowing plant breeders to apply marker assisted selection (MAS) in rice. PCR itself is simple to set up, and requires little hands-on time. However, a crucial limiting step of MAS programs is the reliable and efficient extraction of DNA which can be performed on thousands of individuals. In this study, We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB) is used to extract DNA from leaf tissues for suitable MAS in rice. We followed the standard 2% CTAB extraction method in all the procedure. In addition we used the 1.2 ml 8-strip tube instead of 1.5 ml E-tubes to fit the 8-multichannel pipette and employ the 96 well plate to use the swing bucket centrifuge. Our modified CTAB DNA extraction method offers several advantages with respect to traditional and simple methods. 1) adult leaf samples collected in paddy field are applicable. 2) 96 leaf samples can be homogenized only one-time by using tungsten carbonate bead and 96well block. 3) semiautomatic loading method using 8-multichannel pipette from DNA extraction to electrophoresis of PCR products. 4) our system can extract about 400 leaf samples per day by only one technicion. Therefore, this method could be useful for marker assisted breeding in rice.

항산화 활성이 높은 건강기능성 메수수 ‘동안메’ 육성

김정인,곽도연,고지연,정태욱,이재생,김기영,우관식,송석보,오인석,이명철 한국육종학회 2013 한국육종학회 심포지엄 Vol.2013 No.07

수수는 최근 생활습관병 개선을 위한 곡물로서 각광받고 있어 그 수요가 증가하고 있으나 농가에서 재배하고 있는 수수는 대부분 재래종을 사용하고 있어서 유전적으로 순도가 높지 못하고 수량이 낮아 품종개선이 필요하였다. 이에 따라 국립식량과학원에서는 항산화 활성과 수량성이 높은 건강 기능성 메수수인 ‘동안메’를 육성하였다. ‘동안메’는 2009년 국립농업유전자원센터에서 분양받은 ‘CS-4 LOCAL COLLECTION’을 기본집단으로 하여 분리육종법을 통해 2012년 육성된 품종이다. ‘동안메’는 파종 후 출수까지 평균 71일, 수확까지는 평균 115일인 중생종이다. 키가 약 160cm이고 이삭의 형태는 밀수형이며 밀도는 조밀하며 받침껍질(영)색과 종실색은 짙은 갈색으로 ‘황금찰’보다 좀 더 붉은색을 띈다. 한 주당 이삭수는 3∼4개로, 각 분얼의 이삭이 동시에 성숙하여 일시에 수확할 수 있다는 장점이 있으며 수량성은 약 310kg/10a로 다수성이다. 폴리페놀, 탄닌, 플라보노이드 등 다양한 기능성 성분을 많이 함유하고 있으며 항산화활성(ABTS)이 42.0mgTE/g으로 ‘황금찰’에 비해 30%가 높다. 현곡 천립중은 26.8g, 주당 이삭중은 33.6g으로 무거우며, 도복은 ‘황금찰’보다 강한 편이다. ‘동안메’는 메수수이므로 점질성이 높은 찰수수보다 빵, 과자, 국수 등 가공식품 원료곡으로 이용하기에 적합하여, 건강기능성이 높지만 단순 혼반용으로만 이용되던 수수의 섭취방법을 가공제품 등으로 다양하고 폭넓게 활용이 가능한 품종이다.