해조류 추출물이 섬유아세포의 증식에 미치는 영향

고주영,이지혁,김현수,김형호,전유진,Ko, Ju-Young,Lee, Ji-Hyeok,Kim, Hyun-Soo,Kim, Hyung-Ho,Jeon, You-Jin 한국해양바이오학회 2015 한국해양바이오학회지 Vol.7 No.1

We examined cell regeneration efficiency of brown marine algae living in Jeju coast for search of a novel therapeutic device with cutaneous wound healing materials. The five algae were collected and compared with epidermal growth factor (EGF) as a positive control in the assays of cell proliferation and cell migration of NIH3T3 fibroblast cells. Among the 80% methanol extracts of these brown algae, the two algal extracts from Ishige foliacea and Colpomenia bullosa showed the proliferative effects of the cells similar to the effect of EGF. Besides it was found that Colpomenia bullosa extract significantly enhanced cell migration of NIH3T3 cell. In the study, therefore, we confirmed that the Colpomenia bullosa extract improved proliferation of NIH3T3 cell and a potential candidate for cultaneous wound healing.

삼릉 에탄올추출물의 in vitro 피부 미백 유효성

고주영(Ju-Young Ko),김영철(Young-Chul Kim) 환경독성보건학회 2010 환경독성보건학회지 Vol.25 No.1

The purpose of this study is to evaluate the effectiveness of Scirpi rhizoma ethanol extract (SREE) on skin whitening using in vitro test. In the anti oxidative activities, it was found that SREE contains 38.9㎎/g of polyphenol and 74.5 ㎎/g of flavonoid in total. In the electron donating ability, SREE showed a dose-dependent response, showing a high antioxidative capacity of 86.1% at 1000 ppm. It was found that the maximum permissible level of SREE to Melan-a cells was over 200 ppm, showing a quite low toxicity of SREE against Melan-a cells. Both in the inhibitory measurement for tyrosinase activity and melanogenesis using Melan-a cells, SREE presented a dose-dependent response with excellent efficacy.

PMLSM의 Stator 형상에 따른 추력 리플 특성 해석 및 저감법 고찰

고주영(Ju-Young Ko),이건우(Gun-Woo Lee),정상용(Sang-Yong Jung) 대한전기학회 2013 대한전기학회 학술대회 논문집 Vol.2013 No.7

조기크론병 환자의 진단에 있어서 임상양상과 성격유형의 비교분석에 따른 Peroral Pneumocolon Technique의 유용성

고주영(Ju Young Ko),김상균(Sang Kyun Kim),권명수(Myeong Su kwon),조영기(Young Gi Cho),이현근(Hyun Geun Lee) 대한영상의학기술학회 2010 대한영상의학기술학회 논문지 Vol.2010 No.-

Purpose : In this study, Comparative Analysis was performed on clinical features, laboratory findings and personality types of patients who were diagnosed with Crohn’s Disease, investigated usefulness of PNC technique in the Diagnosis Early Crohn’s D. Materials and methods : It’s subjects included medical record and a questionnaire survey of personality types(50 Questions) was conducted on 22 patients(male: female = 18: 4, average age=25) diagnosed with Crohn’s Disease, among those who underwent small bowels examinations in our hospital from April 2005 to September 2008. Statistical analysis was performed by using SPSS 12.0. Results : Clinical symptoms of patients with Crohn’s disease were as in the following order of abdominal pain (90.9%), and weight loss (81.8%), diarrhea (72.7%), anemia (27.2%), Hematochezia (27.2%), Fever(13.6%). Abnormal laboratory findings include CRP (68.1%), ESR (45.4%) are analyzed by a highly statistically significant as was indicated(P<0.05). However, hemoglobin(27.2%), albumin(22.7%), platelets(13.6%), WBC(13.6%) are analyzed by statistical significance was indicated lower. In the assessment of behavioral pattern, the majority of the patients were likely to obsess about trivial things(81.8%), indecisive(81.7%), introverted personality(81.8%). Conclusion : Comparative Analysis was performed on clinical features, laboratory findings and personality types is expected to contribute to usefullness for Crohn’s Disease by means of more positive use of small bowel series(Peroral Pneumocolon Technique).

위암환자의 위장조영검사 영상분석

고주영(Ju-Young Ko),조영기(Young-Ki Cho),최지원(Ji Won Choi) 한국콘텐츠학회 2010 한국콘텐츠학회논문지 Vol.10 No.9

위절제술을 시행한 위암환자를 대상으로 수술 전 위치확인을 위한 위장조영검사의 영상을 분석하여 위암묘출의 정확도 및 한계성을 알아보고, 이에 따른 대응책을 알아보고자 하였다. 2007년 10월부터 2009년 4월까지 본원에 내원하여 병리조직학적으로 위암으로 확진되어 위절제술을 시행한 조기위암 환자 88명과 조기위암과 비슷한 진행성위암 환자 14명을 대상으로 하여, 수술 전 시행했던 위장조영검사와 수술 후 병리학적 소견을 비교분석하였으며, 영상분석은 10년 이상 근무한 방사선사 2명이 분석하였다. 위암환자 102명의 위절제 후 병리학적 위암위치와 비교를 통한 위장조영검사에서 위암의 발견율은 “확실” 81 예 (79.4%), “실패” 21예(20.6%)로 판정되었다. “실패”로 판정된 21예의 위암형태는 IIb+IIc형이 2예(100%), IIb형이 5예(83.3%)로 발견율이 매우 낮았다. 위암의 전구병변인 장형화생은 위장조영검사에서 92예 (90.2%)의 발견율을 보였다. 위장조영검사의 한계점은 IIb형 및 1.0㎝이하 미소위암의 발견이 어려웠다. 위암과 동반된 장형화생 92예(90.2%)의 발견은 위장조영검사에서 위암의 발견에 중요한 기준으로 평가된다. 위장조영검사의 한계점을 극복하기 위해서는 부위에 맞는 정확한 촬영법 및 풍부한 방사선학적 경험을 통한 미세한 점막의 변화를 확인하는 촬영습관이 위암을 발견하는데 기여할 것으로 사료된다. Despite significant advance in laparoscopy, upper gastrointestinal (UGI) series is still generally carried out for preoperative localization of gastrectomy. The aim of the study was to compare the accuracy of UGI series and postoperative pathological findings in the diagnosis and localization of gastric cancer. A retrospective review was carried out for 102 patients who underwent gastrectomy between October 2007 and April 2009. Preoperative reports of UGI series were compared with postoperative pathology reports and the accuracy of the preoperative reports was calculated. Two radiographer retrospectively reviewed the analysis of UGI series. postoperative pathology reports of the surgical specimens, were compared with the preoperative reports of the location and extent of the tumor were correct in 81 case(sure, 79.4%) and incorrect in 21 case(failed, 20.6%). In 92 case UGI series revealed intestinal metaplasia (90.2%) at consensus review and these results demonstrate the limitation of the UGI series in the diagnosis of type IIb gastric cancer with size less than 1.0cm and the poor detection of gastric cancer is that the overlying mucous membrane often appears to be normal in these patients. In conclusion, UGI series is accurate the detection of the tumor localization and diagnosis of intestinal metaplasia. However, for the overcome with the limitation of UGI series should be used accurate technique for the region of the stomach. To achieve this goal, it is necessary to determine the changes of the mucus membrane of the stomach and UGI series is gaining acceptance as a standard method for preoperative gastric cancer screening.

크론병 환자의 진단에서 식습관 및 성격 특성에 따른 소장조영술 적용에 관한 연구 : Peroral Pneumocolon(PNC) Technique 중심으로

고주영(Ju Young Ko),김상균(Sang Kyun Kim),임성훈(Sung Hun Im),이원균(Won Kyun Lee),김금남(Geum Nam Kim),이우식(Woo Sik Lee),조영기(Young Gi Cho) 대한영상의학기술학회 2007 대한영상의학기술학회 논문지 Vol.2007 No.-

Purpose : In this study, comparison was performed, prior to the small bowel series(SBS), on dietary habit and personality characteristics of younger patients who visited the hospital because of iron deficiency anemia(IDA) or irritable bowel syndrome(IBS) and were diagnosed as with Crohn’s disease, and thereby more positive and closer small bowel series study were conducted on the are as such as ileocecal region, ascending colon, small bowel easier to develop Crohn’s disease, in order to eliminate related problems including a lengthy diagnosis period of Chohn’s disease and misdiagnosis. Materials and Methods : In relation with ‘effect of dietary habit and personality on bowel diseases,’ a questionnaire survey was conducted on 13 patients(male : female =10 : 3, average age=24) diagnosed with Crohn’s disease, among those who underwent small and large bowels examinations in our hospital from January 2004 to June 2003. The questionnaire consisted of 88 items in total among which there were 15, 50, 19, and 4 questions about personal information, dietary habit, personality, obsessive mentality, and behavioral pattern, respectively. Results : As a result, most patients with Crohn’s disease were found to have introverted personality(69%), have 2 meals a day(62%), and prefer meats(23%) and instant food(31%). In the assessment of behavioral pattern, the majority of the patients were likely to obsess about trivial things(77%), indecisive(77%), talk less(69%), and be cautious in handling matters(85%). As for the situations prior to the development of Crohn’s disease, the most common responses were ‘highly busy’ ‘stress-stricken,’ and ‘have trouble sleeping due to anxiety. ‘In addition, as for the causes for worsening of Crohn’s disease, students pointed out the failure to adapt to school life and exams, while workers cited work-related stress. Conclusion : With the inquiries(26 questions) and clinical findings obtained in this study, this study is expected to contribute to shortening of the period for Crohn’s disease diagnosis and minimization of misdiagnosis by means of more positive use of small bowel series(Peroral pneumocolon technique) to a larger degree.

위절제술을 시행한 조기위암환자에서 위치확인을 위한 위장조영검사와 위내시경검사의 비교분석에 따른 진단률 향상에 관한 연구 : 4cm미만의 조기위암 중심으로

고주영(Ju Young Ko),이보영(Bo Young Lee),김상균(Sang Gyun Kim),정재연(Jae Yeon Jeong),김금남(Geum Nam Kim),조영기(Young Gi Cho) 대한영상의학기술학회 2009 대한영상의학기술학회 논문지 Vol.2009 No.-

Purpose : It was prepared to help patients with early gastric cancer who underwent a gastrectomy to detect early gastric cancer by comparing observations from pre-operation UGI series and Endoscopy as well as post-operation pathological observations of type, size, and location. Materials and Methods : It targeted 27 cases who underwent a gastrectomy for early gastric cancer by visiting the department of surgery from February 2007 to September 2008 while the pathological observations of type, size, and location were compared and analyzed through observations from pre-operation gastroscopy and UGI series and medical record after the gastrectomy. Results : Considering the generation frequency after gastrectomy, pyloric antrum was the most with 15 cases (55.6%); specifically, lesser curvature of stomach was the most with 14 cases (51.9%). When 2 accompanied observations were combined, atrophic gastritis and intestinal metaplasia showed high frequencies with 23 cases (85.2%) and 22 cases(81.5%) respectively. Through radiologic severity, 14cases (51.8%) were judged as “concordance” while 13 cases (48.2%) were judged as “discordance”. In the origin site of the 13 cases judged as “discordance” in radiologic severity, pyloric antrum was the most with 9 cases (69.2%); specifically, lesser curvature of stomach was the most with 6 cases (41.6%). Lesser curvature and the anterior wall part of stomach are difficult to be technically depicted minutely during the UGI series, and they are parts where false positive rates are frequently generated. Conclusion : Observation of accompanied intestinal metaplasia(81.5%) is evaluated as the important standard in the discovery of early gastric cancer. Intensive tests are executed for intestinal metaplasia because it is considered as a high risk group and require follow up when suspected. In the result of radiologic severity, there were many observations which looked normal yet it was early gastric cancer in lesser curvature and the anterior wall of stomach including the gastric angle. Only habitual scans of that part with compression and flow technique, while at the same time, confirming the change of mucosa are thought to contribute to discovering early gastric cancer exactly and swiftly in gastric cancer screening tests.

기니아 피그 동물모델에서 삼릉 에탄올추출물의 미백 효과

고주영(Ju-Young Ko),최경화(Kyung-Hwa Choi),김영철(Young-Chul Kim) 환경독성보건학회 2009 환경독성보건학회지 Vol.24 No.3

The purpose of this study is to evaluate the skin whitening effect of Scirpi rhizama ethanol extract (SREE) in an animal model. For the experiment of the study, three brown guinea pigs weighing about 450 g to 550 g were exposed to ultraviolet-B rays on the backs at 500 mJ/㎠ once a week, three consecutive weeks and the total quantity of light was 1,500 mJ/㎠ The artificial tanning spots were divided into six different groups including normal (N), control (C), vehicle control (VC), positive control (PC). experimental 1 (E1, 1% SREE), experimental 2(E2, 2% SREE) groups. Then, 30μL of SREE was transdermaly applied on the artificial tanning spots twice a day and 5 days a week for 8 weeks. With the result of a gross observation, it was found that the degree of pigmentation became apparently thinner in the group applied with E2, compared to the control or the vehicle control group. The melanin index of E2 group was significant lower than the control or the vehicle control group. In the observation with a light microscope, it was found that the degree of melanin pigmentation and S-100 protein expression considerably decreased in the groups applied with SREE, compared to the control or the vehicle control group. With the numerical analysis of melanin pigmentation and S-100 protein expression by using image-analysis software, it was found that the tendency was coincide with the results of microscopic observation.

제주 수산가공부산물 유래 기능성 소재 탐색

강나래,이원우,고주영,김현수,김준성,안용석,고창익,정준범,전유진,Kang, Nalae,Lee, WonWoo,Ko, Ju-Young,Kim, Hyun-Soo,Kim, Junseong,Ahn, Yong-Seok,Ko, Chang-Ik,Jeong, Joon Bum,Jeon, You-Jin 한국해양바이오학회 2014 한국해양바이오학회지 Vol.6 No.2

In this study, we investigated the bioactive substances of the Alcalase hydrolysate obtained from fishery processing by-products in Jeju by measuring bioactivities including radical scavenging acitivty, cytoprotective activity against 2,2-azobis-(2-amidino-propane) dihydrochloride (AAPH), and ACE inhibitory activity. This study is important because of utilization of unused fishery processing by-products in Jeju. The Alcalase hydrolysate was prepared through the hot water extraction and enzymatic hydrolysis, and then further separation of the Alcalase hydrolysate was performed by ultrafiltration using 10 kDa molecular weight cut-off membrane. The Alcalase hydrolysate showed the relatively higher DPPH and peroxyl radical scavenging activity ($IC_{50}$ value; 1.30 mg/ml and 0.888 mg/ml, respectively). Also, the Alcalase hydrolysate showed the ACE inhibitory activity with 1.87 mg/ml of $IC_{50}$ value. These biological activities are increased over 1.2 or 2.5 times through the ultrafiltration of the Alcalase hydrolysate. Therefore, the Alcalase hydrolysate obtained from fishery processing by-products in Jeju and the different molecular weight fractions should be given consideration for food and cosmetics ingredient. Furthermore, this research on the utility of fishery processing by-products might be a useful tool into the industry.

영상검출체 사용에 따른 검사보조자의 피폭정도 측정에 관한 연구

김훈희(Hoon-Hee Kim),고주영(Ju-Young Ko),김영수(Young-Su Kim),유병규(Byung-Kyu Ryu),이현근(Hyun-Geun Lee),조영기(Young-Ki Cho) 대한영상의학기술학회 2012 대한영상의학기술학회 논문지 Vol.2012 No.-

목적: CR카세트와 Portable DR의 후방 피폭의 존재여부를 조사하고, 각각의 영상검출체의 평균 후방선량 및 투과후의 평균 mAs값을 비교분석하여 검사보조자인 방사선사가 받게 되는 후방선량이 적은 검출체를 알아보고자 하였다. 대상 및 방법: 측정기와 X선 Tube까지의 거리를 110cm으로 하고, CR 카세트와 Portable DR을 각각 Chest Decubitus (Non Grid: 71 kVp,10 mAs, Grid: 81 kVp, 10 mAs), T-spine Translateral (Non Grid: 86 kVp, 40 mAs, Grid: 86 kVp, 40 mAs), L-spine Translateral (Non Grid: 90 kVp, 40 mAs, Grid: 90 kVp, 40 mAs)조건으로 지정된 5Point (UP, DOWN, CENTER, LEFT, RIGHT) 지점에 방사선을 5회 조사하여 후방 측에 multifunction meter를 위치시켜 투과후의 mAs의 평균값을 산출하고, 동일한 방법으로 Grid를 올려놓은 후에 총 5회 조사시켜 투과후의 mAs의 평균값을 산출한다. 유리선량계를 동일하게 지정된 영상검출체 후면에 위치시켜 조사조건을 동일하게 하고 총 3회 조사하여 평균값을 산출하여 이를 PASW ver18.0을 이용하여 T-test값을 산출하여 비교분석하였다. 결과: Grid를 올려놓았을 경우에는 Chest Decubitus의 경우 전량 흡수 되었고, T-spine Translateral에서도 전량 흡수된 것으로 나타났다. L-spine Translateral의 경우에는 0.1 mAs가 투과 된 것으로 나타났다. 유리선량계를 이용하여 선량을 측정한 결과 NonGrid상태 에서 Chest Decubitus의 경우 CR카세트에 비해 Portable DR을 이용하였을 경우 평균 50%의 선량 감소효과를 얻을 수 있었고, T-spine Translateral의 경우에는 평균 60%의 선량감소 효과가 나타났다. L-spine Translateral의 경우에는 평균 60%의 선량감소효과가 나타났고,. Grid를 올려놓은 상태에서는 공통적으로 평균 60%의 선량감소효과가 나타났다. 결론: 디지털 영상검출체인 CR 카세트와 Portable DR 모두에서 영상 검사 시 후방 피폭이 발생하며, 그 선량을 측정한 결과 CR카세트에 비해 Portable DR이 최소 50%에서 최대90%의 선량감소 효과를 가지고 있는 것으로 나타났다. 그리고 Portable DR을 이용할 시에는 아래쪽이 다른 곳에 비해 더 많은 선량이 투과 되므로 검사 시 검사보조자는 반드시 영상검출체의 중앙에서 벗어나 좌 또는 우측으로 벗어나서 위치하여야 한다고 사료된다. Purpose: CR Cassette & Portable DR behind the measured average mAs and average dose to play a assist role compared to the test by the radiation technologists receive small doses the rear body detection was investigated. Materials and Methods: Portable DR & CR cassette and in each state Non Grid Chest Decubitus (71 kVp, 10 mAs), T-spine Translateral (86 kVp, 40 mAs), L-spine Translateral (90 kVp, 40 mAs) to a specified (UP, DOWN, CENTER, LEFT, RIGHT) point average is calculated for each survey after. Use the Grid in the same way, and Chest Decubitus (81 kVp, 10 mAs) to the same experimental conditions except for this by using PASW ver18.0 T-test were compared by calculating the value. Results: Chest Decubitus Portable DR compared to CR for Non Grid status in approximately 50% of the dose reduction gained, Grid, if you were used, approximately 60% showed reduction of the dose. In the case of T-Spine Translateral Non Grid state, approximately 98.2%, Grid, if you were used, approximately 60% of the dose effect was reduced. In the case of L-Spine Translateral Non Grid With approximately 80%, Grid If used for more than 60% of the dose effect was reduced. Conclusion: CR cassette, Portable DR imaging in both the posterior exposure occurs. CR dose was measured and compared to the Portable DR up to 90% in at least 50% of the dose was found to have reduced. Portable DR during use and the bottom more than elsewhere, because the test dose transmission inspection assistants necessarily away from the center of the image sensing body left or right beyond that is considered to be located.