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가족성 근위축성측삭경화증 세포모델(G93A, A4V SOD1 점성돌연변이 운동신경세포)의 세포사멸 기전에서 Glycogen Synthase Kinase-3의 역할
고성호,김주한,김명호,유정현,김만호,김현정,이광우,김승현 대한신경과학회 2005 대한신경과학회지 Vol.23 No.2
Background: G93A or A4V mutations in the human Cu/Zn- superoxide dismutase gene (hSOD1) cause familial amyotrophic lateral sclerosis (fALS). However, it has not yet clearly understood how these bring about fALS. We investigated the effects of the G93A or A4V mutations in hSOD1 on the phosphatydilinositol-3-kinase (PI3K)/Akt and glycogen synthase kinase-3 (GSK-3) pathway, and effects of GSK-3 inhibitor on the G93A- or A4V-mutant cells. Methods: To evaluate those effects, VSC4.1 motoneuron cells were transfected with G93A- or A4V-mutant types of hSOD1 (G93A and A4V cells, respectively) with/without GSK-3 inhibitor were compared with them transfected with wild type (wild cells) in cell viability and intracellular signals, including PI3K/Akt, GSK-3, and caspase-3, 24 hours after neuronal differentiation. Results: Compared with wild cells, MTT assay revealed a greatly reduced viability in G93A and A4V cells without GSK-3 inhibitor. However, treatment with GSK-3 inhibitor increased the viability of G93A and A4V cells. Western blotting showed that PI3K and pAkt were decreased, and GSK-3 and caspase-3 were increased in G93A and A4V cells, and that GSK-3 inhibitor treatment reduced caspase-3 but did not affected PI3K, Akt and GSK-3. Conclusions: These results suggest that the G93A or A4V mutations induce inhibition of PI3K/Akt and activation of GSK-3 and caspase-3 resulting the vulnerability to oxidative stress, and that GSK-3 mediated cell death mechanism is important in G93A and A4V cell death.
고성호,김길훈 한국경영교육학회 2019 경영교육연구 Vol.34 No.1
[연구목적]본 연구의 목적은 재무적 요인이 제주지역의 골프회원권 가치에 미치는 영향을 실증분석 하는 것이다. 재무상태와 경영성과가 양호해야 예탁금의 원활한 반환과 골프장의 정상적인 운영이 가능하므로 골프장 경영이 특히 어려운 제주지역의 경우 재무적 요인이 골프회원권 가치에 유의적인 영향을 미칠 것으로 예상된다. [연구방법]골프장품질을 통제하고 재무적 요인인 부채비율, 유동비율, 매출액영업이익률, 이자보상배율 등이 골프회원권 가치에 미치는 영향을 2012년부터 2016년까지 자료를 이용하여 다중회귀분석을 실시하였다. 골프회원권 가치는 기준시가(지방세 과세표준)의 중앙값/분양가격의 중앙값으로 측정하였다. [연구결과]부채비율은 골프회원권 가치에 부정적인 영향을 미치는 것으로 나타났다. 반면 유동비율, 매출액영업이익률, 이자보상배율은 골프회원권 가치에 긍정적인 영향을 미치는 것으로 나타났다. [연구의 시사점]본 연구의 실증분석 결과는 골프장의 재무상황에 따라 골프회원권 가치가 변동한다는 것을 의미한다. 따라서 골프장 경영자는 골프장의 수익성을 높이고 재무적 안전성을 확보함으로써 골프회원권 가치를 유지․제고하는 경영전략을 수립할 필요가 있다. [Purpose]The purpose of this study is to analyze the effect of financial factors to the value of the golf club membership in Jeju. The smooth return of the membership and the normal operation of the golf course are possible only if the financial position and financial performance are good. Therefore, financial factors are expected to have a significant impact on golf club membership value in Jeju, where golf course management is particularly difficult. [Methodology]We analyzed the relationship between the value of the golf club membership and the financial factors such as debt ratio, current ratio, operating margin, and interest coverage ratio from 2012 to 2016. The quality of the golf club was controlled in regression. The value of the golf club membership is measured as a median of the tax base price/median of the initial selling price. [Findings]Debt ratio had a negative effect on the value of the golf club membership. Current ratio, operating margin, and interest coverage ratio had a positive effect on the value of the golf club membership. [Implications]The empirical results of this study indicate that the value of golf club membership varies according to the financial status of the golf club. Therefore, golf course managers need to establish a management strategy that maintains and enhances golf club membership values by securing high profitability and financial safety.