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리튬/공기 이차전지의 충방전 특성과 사이클 특성에 미치는 탄소의 영향 고찰
최현아 ( Hyun A Choi ),고보경 ( Bo Kyung Ko ),심상은 ( Sang Eun Shim ),백성현 ( Sung Hyeon Baeck ) 대한금속재료학회(구 대한금속학회) 2014 대한금속·재료학회지 Vol.52 No.4
Three different carbon blacks (Vulcan, Ketjen Black, and 1:1 mixture of the two) are used as cathode catalysts for a lithium/air secondary battery, and their electrochemical properties are investigated. The surface morphology change after discharge is examined using scanning electron microscopy (SEM), and the discharge products are analyzed using Fourier transform-infrared spectroscopy (FT-IR). In order to measure the electrochemical properties, the charge and discharge measurements are conducted at a constant current density of 0.2 mA/cm2, which monitors the voltage change. Electrochemical impedance spectroscopy (EIS) analyses are also performed in order to examine the change in the charge transfer resistance during the charge-discharge process. Compared with both the Ketjen black and Vulcan, the 1:1 mixture of the two carbon blacks exhibits the best performance as a cathode for the Li/air secondary battery, which results from a synergistic effect that exploits-both carbon blacks.
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전통발효식품으로부터 Chitin 분해 미생물의 분리 및 특성 규명
고보경,최인순,이상현,임채오,이성호,갈상완,최영주 Plant molecular biology and biotechnology research 2004 Plant molecular biology and biotechnology research Vol.2004 No.-
A bacterial strain CJ-3 which produced chitinase was isolated from Korean traditional soy sauce. Using 16S rDNA analysis, the strain CJ-3 was identified as Bacillus atrophaeus. The approximate molecular weight of the putative chitinase enzyme was 31.0 kDa and the enzyme activity was remarkably induced by addition of colloidal chitin (0.5, 1.0, 2.0%). The antioxidant activity was increased 53% by the browning reaction products of B. atrophaeus CJ-3. Escherichia. coli lipopolysaccharides (LPS)-induced production of nitric oxide (NO) was reduced up to 45% by the browning reaction product in RAW264.7 macrophage. Inhibition of cell viability in the presence of LPS was recovered to normal level by the browning reaction product. These results suggest that browning reaction of B. atrophaeus CJ-3 plays an important role for activation of immune system. B. atrophaeus CJ-3 exhibited optimum temperature and pH of 37℃ and pH 7.8~8.0, respectively. The major intracelluar free amino acid was determined to be glutamate.
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벼꽃으로부터 두 개의 calmodulin cDNA 유전자의 분리 및 특성 연구
고보경,고명수,김유정,정일선,최영주 신라대학교 자연과학연구소 2005 自然科學論文集 Vol.14 No.-
Ca^(2+)은 신호전달과정에서 중요한 작용을 하는데 CaM은 이러한 Ca^(2+)이은의 modulator로서 중요한 작용을 하는 것으로 알러져 있다. 벼꽃의 cDNA library로부터 rice genomic DNA(RCaM-2)를 probe로 사용하여 30여개의 Positive clones을 얻어 이 중 두 개의 벼꽃 calmodulin CDNA clone을 분리하였다. RFCaMs CDNA clone의 총염기수는 RFCaM-3은 831p, RFCaM-4는 751bp이고 open reading frame은 각각 149개의 아미노산으로 구성되어 있다. RFCaM-3 과 RFCaM-4의아미노산은 동일하지만 nucleotide 순서는 상당한 차이를 나타내고 있다. RFCaMs CDNA clone의 open reading flame은 protein serine/threonine kinase에 보존되어 있는 catalytic domain을 가지고 있다 RfCaM-3은 5'-untranslated region은 105bp, 3'-untranslated region은 270bp이고 RfCaM-4는 5'-untranslated region은 78bp, 3'-untranslatedregion은 283bp이다. RFCaMs는 Arabidopsis와 70%, potato와는 92%, carrot과는 98%의 유사성을 보였다. Genomic southern blot분석에 의하면 벼의 clamodulin 유전자들이 7-8개의 multigene family로 존재하는 것으로 나타났다. Two different calmodulin (CaM) cDNAs (RFCaM-3 and RFCaM-4) were isolated from a rice flower cDNA library by screening with a rice genomic probe (RCaM-2). Both cDNAs are 90% sequence homologous inside the coding region but are highly divergent outside this region. The polypeptides encoded by RFCaM-3 and RFCaM-4 show identical sequence homology. Comparison of the deduced amino acid sequence of the RFCaMs cDNA clone have identity to Arabidopsis (70%), potato (92%), carrot (98%), and barley and soybean (98%). Southern analysis revealed that both cDNAs are encoded by different genes. Four intense and a few weaker bands were observed in the restriction fragments. Most restriction fragments showed high intensity hybridization signals under stringent conditions, suggesting the presence of a small multigene family for CaM in the rice genome as in other plants.
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Bacillus subtilis CJ-3의 항산화력 및 chitinase 특성규명
고보경,최영주 신라대학교 자연과학연구소 2004 自然科學論文集 Vol.13 No.-
전통발효 식품인 간장으로부터 chitinase 활성이 높으면서 갈변반응을 일으키는 균주인Bacillus subtilis CJ-3를 분리하여 균주의 생화학적 특성을 규명하였다. 분리된 균주는 2% colloidal chitin의 첨가에 의하여 chitinase 활성이 현저히 증가되었으며분자량은 대략 31.0 kDa으로 나타났다. 분리된 균주는 항산화 활성을 나타내었으며 갈변반응에 의하여 DPPH 전자공여능법에의한 항산화력은 9.2%에서 63.0%로 증가하였다. 분리된 균주의 최적 생육조건은 최적온도 37℃, 최적 pH 7.0 및 염 농도는 9% NaCl놈도까지는 잘 생육하는 것으로 나타났다. 분리된 균주의 주요 intracellular 유리아미노산은 glutamate로 나타났다. A bacterial strain CJ-3 which produced chitinase was isolated from Korean traditional soy sauce. Strain CJ-3 was identified as Bacillus subtilis. The Bacillus subtilis CJ-3 was cultured in LB medium containing colloidal chitin (0.5, 1.0, 2.0%) and determined molecular weight. The approximate molecular weight of the putative chitinase enzyme was 31.0 kDa and remarkably induced by addition of colloidal chitin. The antioxidant activity of Bacillus subtilis CJ-3 was increased 53% by browning reaction. The Bacillus subtils CJ-3 has an optimum temperature of 37℃, an optimum pH of 7.0-8.0, and stable concentration of 0.5-9.0% sodium chloride. The major intracelluar free amino acid was determined to be glutamate.
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최문정,하지현,고보경,김태윤,이준영 대한피부과학회 2002 大韓皮膚科學會誌 Vol.40 No.8
Papular xanthoma is a very rare disorder. It is characterized by an eruption of papular and papulonodular xanthomatous elements located on the head, trunk, and extremities. No systemic involvement can be found and blood lipid levels are normal. Histologically there is an infiltrate composed of foamy histiocytes and Touton giant cells in the dermis and characteristically no initial nonlipidized histiocytic phase can be traced in the early development of the lesion. We present a case of papular xanthoma in a 18-month-old boy with typical clinical, histopathologic findings.
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