흰쥐 뇌에서 속후각겉질 손상 후 해마로 투사하는 콜린성 신경로의 변화에 관한 연구

고미희(Mi-Hee Ko),김대성(Dae-Sung Kim),변경희(Kyung-Hee Byun),김재우(Jae-Woo Kim),김명주(Myeong-Ju Kim),오문유(Moon-You Oh),이봉희(Bong-Hee Lee) 대한해부학회 2003 Anatomy & Cell Biology Vol.36 No.1

흰쥐 뇌에서 해마로 투사하는 신경로와 콜린성 신경로를 구축한 뒤 속후각겉질 손상 후에 해마로 투사되는 두 신경로 의 형태학적인 변화를 조사하기 위하여 pseudorabies 바이러스의 Bartha stain을 신경로 추적자로 이용하여 다음과 같은 결과를 얻었다. 안쪽격벽핵 (medial septal nucleus), 줄무늬침상핵 (bed nucleus stria terminalis) 등 10여 핵에서 양성반응이 나타나 해마로 투사하는 신경로를 구축하였으며 무과립 섬겉질 (agranular insular cortex), 중심편도핵 (central amygdala)등의 여러 신경핵들이 해마로 투사하는 콜린성 신경로를 구성하는 신경핵으로 조사되었다. 이 중 신경핵의 종류에 따라 이중양성반응 정도에는 차이를 보여 콜린성 신경로는 신경핵에 따른 차이를 보였다. 속후각겉질 손상 후 10일과 30일에 해마로 투사하는 신경로와 콜린성 신경로의 형태학적 변화를 조사한 결과 내측안쪽무릎핵 (medial geniculate complex, medial part)과 내측배쪽무릎핵 (medial geniculate complex, ventral part) 및 시상(thalamus)등 일부 신경핵에서 양성반응을 보이는 신경세포들의 수가 현저히 감소하였다. 이상의 결과를 종합해 볼 때 Alzheimer’s 질환 모델인 속후각겉질 손상 흰쥐에서 신경로 구성 세포, 콜린성 신경세포 및 신경핵들이 감소되어 치매시의 신경손상이 신경로를 따라 일어 나며 신경세포 수의 감소와 신경전달물질의 감소가 동반 될 것으로 사료된다. The hippocampus is known as involved in learning and memory functions and the entorhinal cortex plays a crucial role as a gateway connecting the several areas and hippocampal formation. Entorhinal cortex lesions have been employed in numerous studies as the Alzheimer’s disease model. The purpose of this study were to identify the CNS hippocampal and cholinergic pathway and to investigate the morphological changes of the hippocampal cholinergic innervations by using the Pseudorabies virus injection into the hippocampus after entorhinal cortex lesions. The pseudorabies virus and double labelled neurons (ChAT and PRV) were distributed at several different nuclei including agranular insular cortex, bed nucleus of stria terminalis, central amygdala, globus pallidus, lateral segment, lateral hypothalamic area, laterodorsal tegmental nucleus, medial septal nucleus, mesencephalic reticular nucleus, periaqueductal gray matter and substantia innominata The morphological changes were observed in the hippocampal cholinergic innervation after entorhinal cortex lesions. These data suggested that the hippocampal cholinergic innervation showed morphological changes throughout the whole brain areas after entorhinal cortex lesion.

장애유형별 구강건강실태에 관한 조사

고미희 ( Mi Hee Ko ) 한국치위생과학회 2004 치위생과학회지 Vol.4 No.1

치과위생사의 직업병에 의한 건강장애요인 인식도 조사

윤미숙 ( Mi Sook Yoon ),송귀숙 ( Gui Sook Song ),고미희 ( Mi Hee Ko ) 한국치위생과학회 2003 치위생과학회지 Vol.3 No.2

국내 말로부터 비폐렴바이러스의 분리 및 면역원성에 관한 연구 II. 국내 분리 말 비폐렴바이러스의 특성 및 면역원성 조사

조길재,김봉환,이두식,오문유,고미희,Cho, Gil-jae,Kim, Bong-hwan,Lee, Du-sik,Oh, Moon-you,Ko, Mi-hee 대한수의학회 1995 大韓獸醫學會誌 Vol.35 No.4

The study was carried out to characterize the properties of Korean isolates of EHV from aborted fetuses and determine envelope protein profiles. The results obtained were summarized as follows; 1. Two strains of EHV was isolated from 2 liver samples among 10 aborted fetuses from which the virus isolation was attempted. 2. Morphological and some enzymatic properties of the Korean isolates of EHV which was designated as $LC_1$ and $LC_2$ was identical to those of a reference strain of Australia-N of EHV-1. The Korean isolates of EHV could be propagated on ED cell culture and they formed typical plaques 1 to 2 days after infection in the ED cells from which typical cuboidal particles of 150~170 nm diameter herpesvirus were observed. The virus could be detected specifically from neucleus and cytoplasm of infected cells by flourescent antibody technique using FITC labelled anti-Aust IV(EHV-1) antiserum. The Korean isolates, $LC_1$ and $LC_2$ were specifically neutralized by anti Aust IV antiserum and reacted positively to CELISA. 3. The structural polypeptides of purified enveloped virions of $LC_1$ and $LC_2$ isolates of EHV were determined by SDS-polyacrylamide gel electrophoresis to identify the envelope glycoproteins. $LC_1$ and $LC_2$ strains revealed 14 glycoproteins ranging in molecular weight from 190 kD to 31 kD while 17 structural proteins of Aust IV(EHV-1), of which 14 were identical to those of $LC_1$ and $LC_2$, were identified. Upon immunoblotting by rabbit antiserum against EHV isolates and EHV-1(Aust IV), 4 immunogenic proteins of $LC_1$ and $LC_2$ were 135 kD, 88 kD, 64 kD and 59 kD, of which 135 kD, 88 kD and 64 kD proteins were also found in Aust IV(EHV-1).

흰쥐 중추신경계내 난소로 투사하는 미주신경로에 관한 연구

김명주(Myeong-Joo Kim),장명세(Myeong-Se Chang),고미희(Mi-Hee Ko),노해숙(Hae-Sook Noh),조해영(He-Young Cho),오문유(Moon-You Oh),이봉희(Bong-Hee Lee) 대한체질인류학회 2000 해부·생물인류학 (Anat Biol Anthropol) Vol.13 No.1

본 실험은 부교감신경의 하나인 미주신경이 난소를 지배하는 신경으로 관여하고 있는지를 pseudora. bies 바이러스를 이용하여 난소신경로와 난소주사후 미주신경정단을 통하여 조사한 연구 보고이다. 이를 위하여 Sprague Dawley계 암흰쥐를 대상으로 pseudorabies 바이러스를 난소에 주사한 무리와 난소 주사후 미주신경을 정단한 우리에서 뇌를 적출하여 pseudorabies 바이러스에 대한 연역조직화학 염색을 시행하여 비교하였다. 본 실험결과 미주신경 중추신경로내의 상위신경핵들이 pseudorabies 바이러스에 대하여 양성반응이 줄어들었거나 관창되지 않는 차이를 보였다 즉 적색핵, 종말판혈관기판, 줄무늬체, 침상핵과 이마엽겉질은 부분적으호 난소의 미주신경로에 관여하고 있으며 미주신경동쭉핵, 고립로핵, 최후영역, 청색반정, 팔옆핵, 코리케퓨즈핵 , 흑색질 및 시각교차위핵은 양성반응이 관찰되지 않아 미주신경으로 투사하는 부교감신경핵으로 조사되었다.

벗나무속 수종간의 핵 리보솜 DNA의 ITS Ⅰ 염기서열 비교

오유성,김기옥,정용환,오문유,한상현,고미희 제주대학교 생명과학연구소 1998 제주생명과학연구 Vol.1 No.-

Comparison of four species (Prunus yedoensis, P. sargentii, P. pendula for. ascendens, P. serrulata var. quelpaertensis) and one cultivar of P. yedoensis were carried out by comparing their nucleotide sequences from the internal transcribed spacers (ITS) I of nuclear ribosomal DNA (rDNA). The length of the ITS I region was ranged from 229 to 236 base pairs (bp). The G+C contents of ITS I ranged from 58.5 to 60.2%. The value of nucleotide divergence across for four Prunus species and one cultivar ranged from 0.4 to 6%. The pairwise distance between P. pendula for. ascendens and P. yedoensis was 0.004 showing the lowest value among any other pairs. On the other hand, the pairwise distance betwen P. yedoensis and cultivated P. yedoensis was 0.026. The nucleotide analysis based on ITS I sequence was considerably similar to previous studies on the basis of random amplified polymorphic DNA (RAPD).

제주도 재래종말에서 혈액 단백질의 유전적 변이에 관한 연구

김세재,정창조,오문유,김규일,김기옥,고미희 濟州大學校 基礎科學硏究所 1992 基礎科學硏究 Vol.5 No.1

In order to clarify the genetic composition and to find possible genetic markers of Cheju native Horses, the genetic variations of the nine proteins (Al, Pi, Es, Hp, Hb, PGD, PGM, MDH, ME) were investigated by the gel electrophoretic techniques. Al: there were two alleles, AlA and AlB. The alleles frequencies of AlA and AlB were 0.398 and 0.602, respectively(X²=0.02, d.f.=2, P>0.9). Pi: Seven Pi alleles(PiF, PiG, PiI, PiL, PiN, PiS and PiU) were detected. The gene frequencies of Pi were PiG 0.136, PiL 0.496, PiN 0.260, and other(PiF, PiI, PiU, PiS) 0.108, respectively(X²= 62.48, d.f.=12, P<0.005). Es: There were three alleles, EsF, EsI and EsS. The frequencies of Es were EsF 0.266, EsI 0.712, and EsS 0.022, respectively(X²= 21.28, d.f.=3, P<0.005). Hb: Three alleles (HbA, HbBI, and HbBII) were found. The gene frequencies of Hb were HbA 0.120, HbBI 0.620 and HbBII 0.260, respectively(X²=2.63, d.f.=3, P>0.25). PGM: There were two alleles, PgmF and PgmS The gene frequencies of PGM were PgmF 0.434 and PgmS 0.566, respectively(X²=0.22, d.f.=2, P>0.75). PGD: There were two alleles, PgmF and PgmS. The gene frequencies of PGD were PgmF 0.823 and PgmS 0.177 respectively(X²=7.53, d.f.=2, P<0.05). Three proteins (Hp, MDH, ME) showed the monomorphic traits in this population. The mean heterozygosity of nine protein loci were calculated as 0.329±0.012.

흰쥐 중추신경계내 난소로 투사하는 미주신경로에 관한 연구

김명주,장명세,고미희,노해숙,조해영,오문유,이봉희 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.1

본 실험은 부교감신경의 하나인 미주신경이 난소를 지배하는 신경으로 관여하고 있는지를 pseudorabies 바이러스를 이용하여 난소신경로와 난소주사후 미주신경절단을 통하여 조사한 연구 보고이다. 이를 위하여 Sprague Dawley계 암흰쥐를 대상으로 pseudorabies 바이러스를 난소에 주사한 무리와 난소 주사후 미주신경을 절단한 무리에서 뇌를 적출하여 pseudorabies 바이러스에 대한 면역조직화학 염색을 시행하여 비교하였다. 본 실험결과 미주신경 중추신경로내의 상위신경핵들이 pseudorabied 바이러스에 대하여 양성반응이 줄어들었거나 관찰되지 않는 차이를 보였다. 즉 적색핵, 종말판혈관기관, 줄무늬체, 침상핵과 이마엽겉질은 부분적으로 난소의 미주신경로에 관여하고 있으며 미주신경등쪽핵, 고립로핵, 최후영역, 청색반점, 팔옆핵, 코리케퓨즈핵, 흑색질 및 시각교차위핵은 양성반응이 관찰되지 않아 미주신경으로 투사하는 부교감신경핵으로 조사되었다. The mammalian ovary has been known as receiving its innervation by sympathetic and sensory neurons of the peripheral nervous system from the brain. Recently, there were several functional reports that the vagus nerves were also regulating the ovarian function, but the vagus nerve had not been identified by clear morphological evidence. A viral transneuronal tracing technique has been used to demonstrate the morphological evidence for the central vagal involvement in ovarian innervation in brain areas. Bartha strain of pseudorabies virus injection was made into the ovary of Sprague Dawley rats. In experimental group, the vagus nerve of the same injection side was removed right after ovarian injection. At five days after initial injection, all the rats were sacrificed and brains were processed for immunohistochemistry. Several central nuclei including hypothalamic paraventricular nucleus showed strong bilateral positive labelings after unilateral injection in control rats, but the positive labelings were disappeared or decreased in several hypothalamic nuclei and nuclei of the vagus nerve. I n conclusion, these results provide the morphological evidence that vagus nerve has neural connection to ovary and by which the central nervous system may maintains the state of ovulation and reproduction as a possible parasympathetic routes in mammals.

RAPD와 핵 리보솜 DNA ITS2 염기서열에 의한 한국산 전나무속 식물의 유전적 변이 및 계통유전학적 분석

정용환,한상현,오유성,고미희,고석찬,김문홍,오문유 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.2

We analyzed the genetic variation and phylogenetic relationship among fourteen taxa of GenusAbies distributed in Korea using random amplified polymorphic DNA (RAPD) and internal transcribed spacer (ITS) 2 sequences of the nuclear ribosomal DNA (rDNA). RAPDs were used for identification of selected taxa by amplification using single 10-mer primers of arbitrary sequence. Fourteen taxa were clearly classified with 10 arbitrary primers, which generated 102 useful polymorphic bands that were evaluated according to the presence or absence of the fragments of equal size. The genetic distance between A. koreana for. chlorocarpa and A. koreana for. rubrocarpa was 0.127, showing the closest among any other pairs. By comparing the base sequences in the ITS2 region of the nuclear rDNA, genetic relationship was assesed among selected taxa. It showed twenty six sites of point mutation (base substitution) in 243 base pairs (bp) of ITS2 region. The G+C contents of ITS2 region were ranged from 58.3% to 61.7%. The value of nucleotide divergence among selected taxa was ranged from 0.4% to 9.8%. These results showed that RAPD and ITS2 sequence analysis was a useful tool for elucidating phylogenetic relationship and selection of specific marker in Abies species.

핵 리보솜 DNA ITS 염기서열 분석에 의한 황칠나무와 일본황칠나무의 계통유전학적 유연 관계

한상현,정용환,고미희,오유성,고석찬,김문홍,오문유 濟州大學校 基礎科學硏究所 2001 基礎科學硏究 Vol.14 No.2

Dendropanax morbifera Le´veille known as a Korean endemic plant and D. trifidus Makino distributed in Japan have been identified as different species. Some biologists have proposed that both plants should be considered as the same species based on their morphological resemblance, uncertainty of discrimination characters and previous molecular evidence revealed by RAPD analysis. Sequences of the internal transcribed spacers (ITS) regions of the nuclear ribosomal DNA (nrDNA) repeats were obtained from each species to reconstruct phylogenetic relationships. The comparison of ITS sequences in the two species through multiple alignments showed similarity between them. Especially, the sequence of Tsushima of D. trifidus Makino revealed 2 insertion/deletion fragments, one o f those was 3 base pairs (bp) longer in ITSⅠ region and the other was 21 bp longer in ITSⅡ region. These fragments appeared only in Tsushima specimen, and moreover, were excluded in family Araliaceae. Also, phylogenetic trees did not even make a difference inter-species but formed mixed branches. With these results, we are convinced that the two taxa are the same species, because they were indistinguishable.