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강희재,Seonggyu Bang,Heyyoung Kim,Ayeong Han,Shuntaro Miura,Hye Sun Park,Islam M. Saadeldin,Sanghoon Lee,Jongki Cho 대한수의학회 2023 大韓獸醫學會誌 Vol.63 No.4
To optimize the most efficient method for porcine in vitro maturation (IVM), we compared the effects of supplementing extracellular vesicles (EVs) derived from porcine follicular fluid (pFF). The cumulus oocyte complexes were grouped into 4 groups with different supplementations as following: pFF (G1), pFF-depleted EVs (G2), EVs (G3) and control (G4) groups. After IVM with different supplementations, maturation rates and the developmental competences of porcine oocytes and blastocyst development were investigated. Additionally, glutathione (GSH) and reactive oxygen species (ROS) levels were measured in mature oocytes. The EVs were isolated and characterized with cryo-TEM and nanoparticle tracking analysis. The pFF significantly affected the maturation rate, whereas the presence of EVs did not show notable difference in the maturation rates. Although there were numerical increases in the measured parameters in EV and pFF-depleted EVs groups, no significant differences were observed between them. The EV group showed similar oocyte maturation rate for both positive and negative control groups. The GSH was not different among the groups, but ROS levels were significantly lower in pFF-supplemented group when compared with other groups with the highest level in the control group. G2 group wasn’t significantly different G1 and G3 group. G3 group wasn’t significantly different from G2 and G4 group. This suggests that EVs in IVM medium which probably effected partially to protect against oxidative stress and potentially enhance the quality of oocytes. This study indicates that the EVs in pFF play a significant role in improving the efficiency of oocyte maturation in porcine.
Ti-P 첨가 극저탄소강의 P입계편석에 미치는 열연권취온도의 영향
강희재,곽재현,진광근,장삼규 대한금속재료학회(대한금속학회) 1992 대한금속·재료학회지 Vol.30 No.10
Hot rolled ultra-low carbon steel strips containing Ti and P have occasionally broken tranversely to rolling direction in the process of pickling. An investigation for this failure was carried out by means of microscopy, mechanical tests and Auger electron spectroscopy. It was found that the failure occurred due to the combination of (1) The phosphorous segregation on ferrite grain boundaries, (2) the impact energy such as tension hunting, and (3) the surface defects which played a role as a notch. The phosphorous segregation was proved to be a major cause for the failure which occurred along the grain boundary. As the amount of phosphorous segregation exponentially increases with an increase in coiling temperature, it must be controlled below 650℃ to prevent the occurrence of the failure.