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강지미 ( Ji Mi Kang ),김정은 ( Jung Eun Kim ),이남호 ( Nam Ho Lee ) 대한화장품학회 2017 대한화장품학회지 Vol.43 No.1
소사나무(Carpinus turczaninowii, C. turczaninowii) 잎 추출물의 멜라닌 생성 억제활성을 B16F10 melanoma 세포를 이용하여 측정하였다. 그 결과, 에탄올 추출물(100 μ g/mL)에서 72.2%의 생성 억제 효과를 확인하였으며, 동일 농도에서 MTT 세포독성은 거의 나타나지 않았다. 분획물(헥산, 에틸아세테이트, 부탄올 및 물)을 제조한 후 실험을 진행한 결과, 에틸아세테이트 분획에서 가장 우수한 활성이 관찰되었다. 에틸아세테이트 분획에서 활성성분을 규명하기 위하여 크로마토그라피를 진행하였으며, 4개의 화합물을 분리 동정하였다; ethyl gallate (1), quercetin rhamnose (2), kaempferol rhamnose (3), quercetin galloylrhamnose (4). 화합물의 구조규명은 핵자기공명분광기 등을 이용하여 이루어졌으며, 4개의 화합물 모두 소사나무 잎에서는 처음 분리된 물질이다. 분리된 화합물을 대상으로 멜라닌 생성 억제활성 실험을 진행한 결과, 화합물 4에서 세포독성 없이 농도의존적인 억제활성을 확인하였다. 또한, 화합물 4는 세포 내에서 티로시나제 발현양을 감소시키고 있음을 ELISA를 통하여 확인하였다. 이상의 결과를 바탕으로, 소사나무 잎 추출물이 화장품에서 미백제로 활용될 가능성이 있다고 판단된다. Melanin synthesis inhibition activities were investigated for the extracts prepared from the leaves of Carpinus turczaninowii (C. turczaninowii) by using B16F10 melanoma cells. As a result, the ethanol extract (100 μg/mL) showed 72.2% inhibition activities without cell toxicities in MTT assays. For the solvent fractions (n-hexane, ethyl acetate, n-butanol, water), the most potent activities were observed at the ethyl acetate fraction. To isolate the active constituents, the ethyl acetate fraction was further purified to afford four compounds; ethyl gallate (1), quercetin rhamnose (2), kaempferol rhamnose (3) and quercetin galloylrhamnose (4). The identification of the isolates was made by spectroscopic data including NMR spectra, and all of the compounds 1-4 were isolated for the first time from the leaves of C. turczaninowii. Anti-melanogenesis activities were studied for the isolates 1-4, and the compound 4 was determined to decrease the melanin synthesis dose-dependently without causing cell toxicities. ELISA measurement indicated that the isolate 4 decreased the contents of cell tyrosinase, a critical enzyme in melanogenesis. Based on these results, the extracts of C. turczaninowii were found to be applicable as whitening ingredients in cosmetic formulations.
Anti-Inflammatory Constituents from the Branches of Pourthiaea villosa (Thunb.) Decne
고하나,강지미,김형민,한종헌,이남호 대한화학회 2017 Bulletin of the Korean Chemical Society Vol.38 No.7
In this study, the extract from Pourthiaea villosa Decne branches was investigated and their anti-inflammatory constituents were identified. The prepared ethanol extract was fractionated successively to afford n-hexane, ethyl acetate (EtOAc), n-butanol, and aqueous layers. In the examination of the anti-inflammatory activities, EtOAc layer was observed to have good nitric oxide production inhibitory property in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. Phytochemical studies for EtOAc layer led to isolation of eight constituents such as ursolic acid (1), arjunolic acid (2), daucosterol (3), myricanol (4), gallic acid (5), myricetin (6), myricitrin (7), and quercitrin (8). The isolate 4 efficiently inhibited nitric oxide production in a dose-dependent manner in LPS-induced RAW 264.7 cells. The production of inducible nitric oxide synthase (iNOS) enzyme protein was decreased by compound 4, which showed that the observed NO inhibition could be interconnected to down-expression of iNOS protein. In addition, compound 4 reduced the production of TNF-α, IL-1β, and IL-6 in a concentration-dependent manner. This study demonstrated that the P. villosa extract containing myricanol (4) could be considered potential anti-inflammatory agents in pharmaceutical or cosmetic industries.
조연정,현주미,강지미,김창윤,이남호,Jo, Yeon Jeong,Hyun, Ju Mi,Kang, Ji Mi,Kim, Chang Yun,Lee, Nam Ho 한국생약학회 2022 생약학회지 Vol.53 No.3
In this study, we investigated anti-oxidative and anti-inflammatory efficacy, and identified their constituents from Brassica napus L. (Korean name: Yuchae) whole plant. Upon the anti-oxidative activities screening, the ethanol extract exhibited potent DPPH and ABTS<sup>+</sup> radical scavenging activities. On the anti-inflammation studies using LPS-induced RAW264.7 cells, the extract inhibited the production of NO and pro-inflammatory cytokines (TNF-α, IL-1β, IL-6) effectively. To identify major constituents of B. napus extract, further purification was performed and led to isolation of two compounds; isorhamnetin 3,7-O-diglucoside(1) and isorhamnetin 3-O-glucoside(2). Quantitative analysis by high pressure liquid chromatography (HPLC) determined the flavonoid 1 as the major constituent. Isolated compounds showed DPPH radical scavenging effects and decreased NO levels without causing cell toxicities. These results indicate that the extract of Yuchae, a rich plant resource in Jeju Island, could be potentially applicable as an anti-oxidative and/or anti-inflammatory ingredients.