난알부민으로 유도된 천식 마우스에서 식방풍(植防風)의 알레르기 면역반응 조절효과 연구

강석용 ( Seok Yong Kang ),오태우 ( Tae Woo Oh ),김진웅 ( Jin Woong Kim ),박용기 ( Yong Ki Park ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.6

Objectives: The root of Peucedanum japonicum Thunberg (Peucedani Japonici Radix; PJR) has been traditionally used as an herbal medicine for the treatment of anti-headache, anti-paralysis, anti-cancer, vascular protection, and blood pressure regulation. In this study, we investigated the anti-allergic effect of PJR water extract on ovalbumin (OVA)-induced allergic asthma in mice. Methods : Mice were sensitized at days 1, 8 and 15 with OVA and airway challenged at days 22, 24, 26, 28, and 30 to induced allergic asthma. PJR-W extract at doses of 100 and 300 ㎎/㎏/body weight (bw) was orally administered during OVA challenge once per a day. The levels of allergic mediators such as immunoglobulin (Ig) E, and Th1/Th2 cytokines (IFN-γ and IL-4) were measured in the sera of mice by ELISA. The histological change of lung tissue was observed with hematoxylin and eosin (H&E) staining. Results : The administration of PJR-W extract significantly decreased the serum levels of IgE, IL-4, and IFN-γ compared with those of OVA control group. In H&E staining, PJR-E extract inhibited OVA-induced airway inflammation and the inflammatory cells infiltration in the peribronchial regions of the lung. Conclusions : These results indicate that PJR-W extract has an anti-inflammatory and anti-allergic effect on allergic response through the down-regulation of allergic mediators, suggesting that this herb may be used as a useful source for the treatment of allergic inflammatory diseases such as asthma.

곽향(藿香)의 난알부민으로 유도된 천식 마우스에서의 천식개선 효능연구

강석용 ( Seok Yong Kang ),박용기 ( Yong Ki Park ) 대한본초학회 2015 大韓本草學會誌 Vol.30 No.3

Objectives : In this study, we investigated the effects of Agastachis Herba water (AH-W) extract on compound 48/80-induced mast cell degranulation and histamine release in human mast cells and also anti-asthmatic effect of AH-W extract on ovalbumin (OVA)-induced asthma in mice. Methods : Human mast cells, HMC-1 were treated with AH-W extract in the presence or absence of compound 48/80 (C48/80). Mast cell degranulation was observed by microscope, and the histamine release was measured in culture medium by ELISA. For preparation of asthmatic in vivo model, mice were sensitized (0, 7, and 14 days) with OVA and airway challenged (21, 23, 25, 27, and 29 days). AH-W extract at doses of 100 and 300 mg/kg/body weight was orally administered during OVA challenge once per a day. The levels of immunoglobulin (Ig) E, and Th1/Th2 cytokines, IFN-γ and IL-4 were measured in the sera of mice by ELISA. The histopathological change of lung tissues was observed by hematoxylin and eosin (H&E) and Periodic Acid Schiff (PAS) staining. Results : The treatment of AH-W extract significantly decreased the mast cell degranulation and histamine release in C48/80-stimulated HMC-1 cells. In addition, The administration of AH-W extract at does of 100 and 300 mg/kg significantly decreased the serum levels of OVA-specific IgE compared with those of OVA control group. In H&E and PAS staining, AH-W extract inhibited OVA-induced airway inflammation, and inflammatory cells infiltration, and also histopathological damages on lung tissues such as bronchiole epithelial desquamation, goblet cells hyperplasia, and mucin releasing. Conclusions : These results indicate that AH-W extract may improve asthmatic symptoms through mast cell stabilization and inhibiting the lung inflammation in bronchial asthma.

난알부민 유도 알레르기 면역반응에 대한 당삼(黨參)에탄올추출물의 효능연구

강석용 ( Seok Yong Kang ),정진기 ( Jin Ki Jung ),이상국 ( Sang Kook Lee ),이승호 ( Seung Ho Lee ),박용기 ( Yong-ki Park ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.2

Objectives: The root of Codonopsis pilosula (Fr.) Nannf. (Codonopsis Pilosulae Radix) has been traditionally used as a oriental medicine with an anti-thrombotic, antidiabetic, anticancer, and anti-gastric ulcer effects and immunological adjuvant. In this study, we investigated the effect of 70% ethanol extract of Codonopsis Pilosulae Radix (CPR-E) on ovalbumin (OVA)-induced allergic responses in mice. Methods : Mice were sensitized (1, 8, and 15 days) with OVA and airway challenged(22, 24, 26, 28, and 30 days) to induced allergic responses. CPR-E extract at doses of 50 and 100 ㎎/㎏/body weight was orally administered from days 21 to 30 consecutively. The levels of allergic mediators such as histamine, OVA-specific immunoglobulin (Ig) E, and Th1/Th2 cytokines such as IFN-γ and IL-4 were measured in the sera of mice by ELISA. The histological change of lung tissue was observed by hematoxylin and eosin (H&E) staining. Results : CPR-E extract significantly decreased the serum levels of histamine, OVA-specific IgE, and IL-4 compared with those of OVA control group, but significantly increased the serum level of IFN-γ. Based on H&E staining, CPR-E extract inhibited the infiltration of inflammatory cells into lung tissues with histological changes. Conclusions : These results indicate that CPR-E extract has anti-inflammatory and anti-allergic responses through regulating the cytokine balance, suggesting that the extract may be useful for the treatment of allergic inflammatory diseases such as bronchial asthma and allergic rhinitis.

난알부민으로 유도된 천식 마우스에서 상백피 추출물의 면역조절효능 연구

강석용 ( Seok Yong Kang ),우은란 ( Eun Rhan Woo ),박용기 ( Yong Ki Park ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.4

The root bark of Morus alba L. (Mori Cortex Radidus; MCR) has been traditionally used to reduce heat from the lungs, soothe asthma, and edema and to promote urination. In this study, we investigated the effect of MCR ethanol extract on ovalbumin (OVA)-induced allergic asthma in mice. Methods : Mice were sensitized at day 0, 7 and 14 with 0.2% OVA and then airway challenged at day 21, 23, 25, 27 and 29 to induce allergic asthma. MCR extracts at doses of 50 and 100 ㎎/㎏ body weight (bw) were orally administered during OVA challenge once per a day. The levels of allergic mediators such as histamine, OVA-specific IgE, IFN-γ and IL-4 were measured in the sera of mice by ELISA. The histopathological change of lung tissues was observed with hematoxylin and eosin (H&E) staining. Results : MCR extract significantly decreased not only the serum levels of histamine, OVA-specific IgE, and IL-4 compared with those of OVA control group, but significantly increased the serum level of IFN-γ. In H&E staining, MCR extract inhibited the infiltration of inflammatory cells and bronchiolar damage with epithelial thickening in lung tissues of OVA-induced asthma mice. Conclusions : These results indicate that MCR extract inhibits lung damage by asthma through regulating the allergic immune response, suggesting that MCR may be used as a useful agent for the treatment of allergic asthma.

알레르기 비염 한약제제 KOB와 주요 구성약물인 황기(黃氣)의 난알부민 유도 알레르기 비염 마우스에서의 항알레르기 효능 연구

강석용 ( Seok Yong Kang ),홍승욱 ( Seung Ug Hong ),박용기 ( Yong Ki Park ) 대한본초학회 2012 大韓本草學會誌 Vol.27 No.4

Objectives : In this study, we investigated the effect of KOB, a polyherbal medicine for allergic rhinitis and its main herb, Astragali Radix on allergic responses in ovalbumin (OVA)-induced Allergic rhinitis(AR) mice. Methods : Sprague Dawley (SD)-rats were orally administrated with KOB (500 ㎎/㎏), Astragali Radix water extract (ARW, 100 ㎎/㎏) or anti-histamine drug, dosodium cromoglycate (50 ㎎/㎏) as a reference drug, and then intraperitoneally injected with compound 48/80 (8 ㎎/㎏). Rats were measured the mortality and serum levels of histamine. BALB/c mice were orally administrated with KOB (500 ㎎/㎏), ARW (100 ㎎/㎏) or anti-histamine drug, Ketotifen (10 ㎎/㎏) as a reference drug, followed by sensitization and challenge of OVA. Mice were measured the serum levels of histamine and IgE, and observed histopathological changes of nasa mucosa H&E staining. Results : KOB and ARW significantly decreased the mortality and the serum levels of histamine in compound 48/80-induced anaphylatic rats. KOB and ARW also decreased the serum levels of histamine and IgE in OVA-induced AR mice, and inhibited histopathological changes of nasal mucosa with inflammation and the eosinophils infilteration. Conclusions : These data suggest that KOB has a strong anti-allergic effect through the inhibitory property of Astragali Radix, the main component of KOB against allergic responses in allergic rhinitis.

본초 귀경에 따른 상소한약의 당내성 유발 마우스에서의 개선 효능 비교 연구

강석용 ( Seok Yong Kang ),박용기 ( Yong Ki Park ) 대한본초학회 2014 大韓本草學會誌 Vol.29 No.5

Objectives : To prove the channel-tropism theory of herbal medicines on diabetes mellitus as emaciation-thirst disease in Korean Medicine Theory, we investigated the selective therapeutic effects of Mori Cortex Radidus (MCR), Schisandrae Fructus (SF), Anemarrhenae Rhizoma (AR) for the upper emaciation on different organs in high fat and high sucrose (HF/HS) feeding-induced prediabetic mice. Methods : Diabetes in C57BL/6 mice was induced by the administration of high fat (45 kal%) and high sucrose (32 kal%) for 8 weeks, and them treated with each extract at 250 or 500 mg/kg body weight for 4 weeks (once a day). Oral glucose tolerance test and body weight was measured once a week. Insulin, total cholesterol, triglyceride, γGTP, GOT and GPT were measured in the sera of all mice. Histopathological changes of different organs, lung, heart, pancreas, stomach, liver, and kidney were observed by H&E staining. Results : The results revealed that MCR extract inhibited the impaired glucose tolerance and lung damage, and increased serum insulin levels in HF/HS-induced prediabetic mice. SF extract inhibited the impaired glucose tolerance and lung damage, increased serum insulin levels, and decreased serum triglycerige levels. Meanwhile, AR extract inhibited the impaired glucose tolerance and lung damage, and decreased serum levels of insulin, total cholesterol and triglycerige levels. Conclusions : These results demonstrated that MCR, SF, and AR extract as the upper emaciation herbal medicines were followed their channel-tropism theory like a lung, and may have a selective therapeutic potential for control of diabetic stage.

알러지성 비염 한약제제 KOB의 랫드에서의 13주 반복 경구투여에 의한 독성 연구

박용기 ( Yong Ki Park ),박영철 ( Yeong Chul Park ),강석용 ( Seok Yong Kang ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.1

Objectives : To evaluate the safety of KOB, a polyherbal medicine for allergic rhinitis, we conducted a subchronic toxicology study, Methods : Dried extract of KOB(Lot. No. 11003, yield : 41.1%) was prepared from GLP company (Hanpoong Pharm & Food Co., Ltd). KOB was repeatedly administrated orally of male SD rats at daily dose levels of 500 (G2), 1250 (G3) and 5000 (G4) ㎎/㎏/day for 13 weeks. We recorded the clinical signs of toxicity, body weight, food intake/consumption, optometry, urine analysis, organ weights, hematology, and conducted serum biochemical analysis, necropsy, gross and histological changes in target organs of Sprague-Dawley rats, and clinical chemistry analysis, Results : Neither death nor any toxicological signs were obserbed in KOB at all doses of 500, 1250 and 5000 ㎎/㎏/day during the administration period for thirteen-week. Furthermore, there was no difference in body weight and food-take consumption, optometry, necropsy, organ weight, gross pathological findings, and urine analysis among the groups of rats treated with different doses of KOB, during at the observation period for thirteen-week. The hematological analysis and clinical blood chemistry data were revealed no toxic effects from repeated-dose administration of KOB in rats during the observation period, Conclusions : Based on these results, the no observable adverse effect level (NOAEL) of KOB was considered to be 5000 ㎎/㎏/day for male rats under these study conditions.

고지방·고탄수화물 식이와 Streptozotocin-유도 당뇨병 흰쥐에서 삼릉추출물의 개선효능 연구

강안나,강석용,송미영,박용기,Kang, An Na,Kang, Seok Yong,Song, Mi-Young,Park, Yong-Ki 한방비만학회 2016 한방비만학회지 Vol.16 No.2

Objectives: This study was designed to evaluate the anti-diabetic effects of Spargaium stoloniferum Buchanan-Hamilton (Sparganii Rhizoma, SR) extract on diabetic rats. Methods: Diabetes was induced with Sprague-Dawley rats by high fat/high sucrose (HF/HS) diet for 4 weeks and injection of a single low dose of streptozotocin (STZ; 35 mg/kg). SR water extract at 500 mg/kg was orally administrated once a day for 4 weeks. Body weights, food and water intakes and urine volumes were measured. The levels of glucose, insulin, total cholesterol, glutamic oxaloacetic transaminase and glutamic-pyruvic transaminase (GPT) were measured in the sera of rats. Histological changes were observed in pancreas, liver, and kidney tissues by H&E staining. Results: The administration of Sparganii Rhizoma extract at 500 mg/kg in diabetic rats did not shown a significant difference in body weight changes and GPT levels, but showed meaningful changes in an increase of urination volume, and decrease of serum glucose and insulin levels. Total cholesterol and GPT levels were also significantly decreased after SR extract administration in diabetic rats. Furthermore, the abnormal changes of pancreas, liver and kidney were also improved by Sparganii Rhizoma extract administration. Conclusions: These results indicate that SR extract can improve HF/HS-diet and STZ-induced diabetic damages in rats through inhibition of the blood glucose and insulin increase.

Streptozotocin으로 유도된 당뇨 흰쥐에서 하소에 사용되는 한약의 항당뇨 효과 비교 연구

오태우 ( Tae Woo Oh ),강석용 ( Seok Yong Kang ),김경호 ( Kyung Ho Kim ),송미영 ( Mi Young Song ),박용기 ( Yong Ki Park ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.5

Objectives: To prove the channel-tropism theory in herbology, we investigated the anti-diabetic effect of six herbal plants used for lower wasting-thirst in streptozotocin-induced diabetic rats. Methods: Diabetes was induced in male Sprague-Dawley rats by consecutive injection of streptozotocin (30 mg/kg i.p.) for 5 days. The rats were divided into normal control, diabetic control, and diabetic treatment with Lycii Radicis Cortex (LRC, 300 ㎎/㎏); Corni Fructus (CF, 300 ㎎/㎏); Bombyx Batryticatus (BB, 50 ㎎/㎏); Lycii Fructus (LF, 300 ㎎/㎏); Phellodendri Cortex (PC, 300 ㎎/㎏); Epimedii Herba (EH, 300 ㎎/㎏); and glibenclimide (10 ㎎/㎏) as a reference drug. Herbal extracts or reference drug were administered orally for 28 days. The changes of body weight, food intake and water intake, and serological markers such as blood glucose, serum total cholesterol, triglyceride (TG), blood urea nitrogen (BUN), and creatinine (Cr) were measured. Results: The decrease of body weight and the increase of food and water intake in STZ-induced diabetic rats was improved by the administration of CF and LF. Also, the enhancement of blood glucose and serum total cholesterol, TG, BUN and Cr in STZ-induced diabetic rats was significantly inhibited by the administration of CF, BB, LF and glibenclimide. On the other hand, EH strongly inhibited the increase of BUN and Cr in the sera of STZ-induced diabetic rats. Conclusions: These results suggest that among six herbal medicines used lower emaciation of emaciation-thirst disease, CF, BB, LF and EH show a characteristics including the channel-tropism theory.

Streptozotocin 유도 당뇨 흰쥐에서 주요 장부 간 조직학적 변화 비교 연구

오태우 ( Tae Woo Oh ),강석용 ( Seok Yong Kang ),박용기 ( Yong Ki Park ) 대한본초학회 2013 大韓本草學會誌 Vol.28 No.6

Objectives : This study was conduct to compare of histological changes on four target organs which related with diabetes in streptozotocin-induced diabetic rats. Methods : Diabetes was induced in male Sprague-Dawley rats by consecutive injection of streptozotocin (STZ) at different doses of 30, 40 and 50 ㎎/㎏ for 5 days. After 4 weeks, all rats were sacrificed, five different organs such as pancreas, liver, kidney, and lung were isolated and observed their histological changes by hematoxylin and eosin (H&E), Periodic acid-Schiff (PAS) and Masson`s trichrome staining. The changes of body weight, blood glucose, and food and water intake were also measured. Results : The multiple administration of STZ was induced diabetes in rats with hyperglycemia, decrease of body weight, increase water and food intake, and histopathological changes of target organs, compared with those of normal rats in both dose-dependent and time-dependent manner. In histological analysis, pancreas was showed decrease of the islet numbers with beta-cell loss. Kidney showed morphological damage with glomerulus hypertrophy, and also lung was showed bronchial epithelial damage with inflammatory cells infiltration. In liver, the portal vein and hepatic artery could not observed, and showed inflammatory cell infiltration with liver fibrosis. Conclusions : These results suggest that the increase of the capacity of STZ, each of the more chronic disease, it can be seen that the damage was deep. Thus, evaluate the resulting drug appropriate depending on the purpose of the model is expected to be selected.