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윤여창,김태종,이중복,이기수,윤화중,황도윤 건국대학교 동물자원연구센터 1998 動物資源硏究誌 Vol.19 No.-
Exteroxigenic Escherichia coli(ETEC) is a major cause of diarrhea in neonatal and weaning piglets. The incidence of ETEC in piglets with diarrhea was investigated by detecting the K88, K99 and 987P antigens from the isolated strains of Escherichia coli(E.coli). This study was carried out to investigate the biochemical characteristic, antibiotic susceptibililty and development of E.coli vaccine against diarrheal disease in piglets. The results obtained from this study were summarized as follows: 1. 24 strains of K88 positive ETEC were isolated from 64 E.coli. 2. In antibiotic susceptibility test, K88 positive ETEC showed high sensitivity to imipenem but resistance to penicillin and vancomycin. 3. 16 strains were positive for heat labile toxin, but not shown hemolysis. 4. The purified pilus of K88 positive ETEC had molecular weight of 28.5 KDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. 5. Serum antibody responses to immunogen in guinea pigs given experimental oil and gel emulsion vaccine peaked at 4 and 6 week after vaccination. 6. In the mean hemagglutination inhibition titer to antigens of guinea pigs, oil emulsion pili vaccine was 1:1280 and those given gel emulsion pili vaccine was 1:640 respectively. 7. Agglutination titers in pregnant gilts given the oil emulsion pili vaccine were significantly higher compared to those of control groups. 8. The protective efficacy of the oil emulsion pili vaccine was 77.3% under field condition.