수도종자의 방사선조사에 있어서 열처리의 효과

韓昶烈,元鍾樂,崔光泰 韓國作物學會 1971 Korean journal of crop science Vol.- No.10

방사선조사시 열처리의 효과를 알기 위하여 수도종자에 방사선조사시 열전후처리를 행하였던바M1 세대의 생물학적인 반응 및 M2 세대의 변이율에 대해 몇가지 결과를 얻었다. 1. 방사선선량 20kR에서 열전처리는 방사선단독조사에 비해 생물학적인 장해를 적게하고 변이율을 증가시켰다. 2. 방사선단독조사 및 조사전열처리에 비해 heat shocking으로서 열후처리하는 것은 변이율을 증가시켰다. 3. 열전처리에 의해 M1 세대의 장해가 적고 변이율이증가되는 가장 효과적인 열처리온도 및 시간은 60℃ 에서 30분간이었다. 4. 열처리는 방사선단독조사에 비해 mutation spectrum을 달리했다. For the purpose of finding out the effect of heat treatment on biological response and mutation rate, rice seeds were heat treated before and after gamma irradiation. 1. At a dose of 20 KR, pre-irradiation heat treatment showed reduced biological damage and increased mutation rate as compared with non-heat treatment. 2. Mutation frequency was increased in post-treatment of heat shock than in pre-irradiation heat treatment and non-heat treatment. 3. Pre-irradiation heat treatment at 60~circC for 30 minutes markedly reduced the biological damage and increased the mutation rate. 4. Mutation spectrum in heat treatment was different from non-treatment.

Persicaria senticosa Nakai 의 이 Dimorphism 에 관한 연구

한창열 (Chang Yawl Harn) 한국식물학회 1960 Journal of Plant Biology Vol.3 No.1

HARN. Chang Yawl (Chonnam U. Kwangju, Korea): Siudies on the dimorpism of the Perisicaria senticosa Nakai-Kor. jour. Bot. 3(1) 16-25 During his researches regarding the morphological and physiological properties of Polygonaceae, the author has found that the species, Persicaria senticosa, aiso besides the heterostylous plants of Polygonum family, Fagopyrum esculentum, and Persicaria japonica which was recently verified by the author as dimorphic, shows the typical floral structure of heterostylism, the description of which is not found in taxonomical works. Further research on this species have revealed that this plant, despite pessessing characteristic structulal dimorphism, does not exhibit even the slight signs of heterostylcus properties physiologically. This is a deviation from the usual behavior of normal heterostylous plants. What is interesting is the fact that the physiological behavior of this speeies is quite contrary to that of P. japonica which is considered to be the most highly speciaiized dimorphic plant. Thus it Is assumed that if some species of this family had taken a heterostylic form in its course of development from autogamy to allogamy, P. seoticosa would be the least differentiated type of dimorphic forms among the three heterostylous plants, including buckwheat, of this family. The results obtained in this experiment are summarized as follows: 1) P. senticosa has two forms of flower, one, long style-short stamened; the other, short style-long stamened. Not only conspicuous is this primary difference, but the secondary difference, such as pollen grain size, is noticeable between long and short styled individuals, thus expressing structurally the definite trait of a dimorphic plant. 2) Structural alteration of floral parts towards dimorphism has preceeded far less in comparison with those of the P. japonica and F. esculentum. 3) Elaborate studies on fertility reveal that this species dose not show the slighbest sign of the physiological chararteristics of dimorphic plants. In other words, regardless of the modes of combinations, legitimate and illegitimate, fertilization and fruit setting flourish unimpaired. 4) Growth of pollen tubes apparently parallels the results in the fertility, tubes reaching ovary approximately 30 minutes after pollination both in legitimate and illegitimate combinations. Pollen tube penetration appears to be comparatively rapid. 5) A slight differenre in the growth of pollen tube seems to exist between legitimate and illegitimate combinations, legitimate union giving slightly faster tube penetration. 6) In the present experiment it was clarified that P. senticosa, known to possess one form of flower in taxonomy, is in reality dimorphic plant having two of flowers. Although this species if definitely heterostylous in floral structure, physiological evidence and pollen tube behavior show that the differentiation of this plant toward the dimorphism has apparently proceeded slightly except for some parts of floral organ. In ordinary heterostylous plants it is a matter of common occurrence that when iilegitimately combined, there is poor or no fertility, Contrary to the universal propety of heterostylous plants, no difference is oberved in the fertility and pollen tube gowth between the legutumate and illegitimate combinations in the case of P. somkiosa. Compared to the P. japonica and F. esculentum, whichare supposed to have undergone high degree of dimorphic differentiation, it is an unavoidable conclusion that P. senticos has not yet developed as a heterostyle plant except for some of its floral parts. If P. japonica is assumed to be the most diffentiated type of heterostylous plant, thenthe P. senticosa would be regarded as the primitive, retaining still the self-fertile nature so common to the Polygonun genus. In nature, however, this plant has a better chance to be pollinated legitimately owing to the two forms of flowers than to be pollinated illegitimately. The

고추염색체의 복합전좌합성에 (複合轉座合成) 관한 연구 1 : 전좌 (轉座) homo 개체의 선발

한창열,원종락 한국육종학회 1975 한국육종학회지 Vol.7 No.2

Persicaria Japonica ( MISSNER ) Gross et Nakai 의 이형화와 (二型花) 수정력에 관한 연구

한창열 (Chang Yawl Harn) 한국식물학회 1960 Journal of Plant Biology Vol.3 No.1

HARN, Chang Yawl : Studies on the dimorphism and Fertility of Persicaria japonica (MEISSNER) Cross et Nakai. Kor Jour. Bot. 3(I) 1-15 1960 Numerous investigations, since the works of DARWIN, have teen made regarding the heterostylous plants by JOST (1907), CORRENS (1924), LAIBACK (1924), LEWIS (1943), and many others. Studies on the heterostylous Polygonum, however, were not reported except for the buckwheat, Fagopyrum esculentum, which was investigatedy by SCHOCH-BODMER (1930), EAST (1934), FROLOVA & Co-Workers (1946), MORRIS (1947, 1951), TATEBE (1949, 1951, 1953), present author (1957), and others. It is because no heterostylous species, besides buckwheat, have been known to exist in the Polygonum family. The author, during his studies on both heterostylism and fertility of Polygonaceae, has found that the species, persicaris japonica (Meissener) Gross et Nakai, is not dioecious as has been known in taxnonomy, but in reality heterostylous both morphologically and physiologically. It was found that this Plant, regarded by taxonomist, as a male plant setting no seed, actually set seed (botanical fruit) when legitimate combination was made. Since his brief report on the dimorphic Phenomena of this plant in 1956, the author`s further research on the manner of fertilization has revealed that this species is a peeuliae type whose dimorphism has undergone extreme specialization structurally and physiologically, the short-styled individual behaving in nature as a male plant and the long-styled individual, as female, whereas in controlled pollination the plant shows highly differentiated typical dimorphism. When compared with the other dimorphous species of this family, F. esculentum and P. srnticosa. it has been clarified that these three species differ in the degree of differentigtion of their dimorphism morphologically and physiologically. That is, P. japonica has developed such a high specialization as to mislead the taxonomists, while P. senticosa shows almost no noticeable difference between long- and shortstyled individuals retaining most of the inherent physiological character common to the genus except for the fact that it has two forms of flowers. F. esculentum appears to have taken the intermediate position in every respect. The results obtained in the present experiment are summarized as follows : 1) P. japonica has two kinds of individuals, one long style-shore stamened: the other, short style-long stamened. The floral structure of this plants shows typical characteristics of dimorphic heterostylism. The differentiation between the two forms of flower has proceeded so highly both in primary and secondary difference of flower structure that this may be regarded as the most specialized form of dimorphism. 2) The differences of floral structure between the long and short styled individuals are remarkable coupared with the other dimorphic species of the family. 3) The stament of long styled plants show the sign of deteriolation whereas these of the short styled flower are well-developed. 4) When lecgitimate combinations are made, both L- and S-styled individuals are fertilized well and set seed (fruit), while in the illegitimate combination no fertilization and seed setting occur. Physiologically this species exhibits the typical behavior of dimorphic plants. 5) The self-fertile character, so common in other species of the other non-heterostyle Polygonum familly, has disappeared completely. 6) Under natural conditions, no or few seed setting is observed in short styled individuals that behave as if they were male plants. 7) In hand pollination, the combinations of both L x S aud S x L alike yield relatively good fertility and seed-formations, the behavior of short styled individuals in artificial pollination differing remarkably from that in nature. 8) Under controlled pollination. L x S combination sets far more seed than in the combination of S x L. In the S-styled individuals, the fertilized flower has

식물의 염색체상호전좌의 (染色體相互轉座) 이용

한창열 (Chang Yawl Harn) 한국식물학회 1973 Journal of Plant Biology Vol.16 No.1-2

The spontaneous and induced reciprocal translocations of chromosomes in higher plants were reviewed and discussion was made on the utilization of this chromosome aberration in the production of seedless hybrid seeds in vegetable crops.

당근의 組織培養에 關한 硏究

韓昶烈(Changyawl HARN),權臣漢(Sinhan KWON),任建爀(Kunhyuk IM),金光植(Kwangsik KIM),金成器(Sungkih KIM) 한국원예학회 1968 Horticulture, Environment, and Biotechnology Vol.3 No.-

식물의 자가불화합성, 최근의 진보

한창열,한지학 한국식물생명공학회 1994 식물생명공학회지 Vol.21 No.5

Many flowering plants possess genetically controlled self -incompatibility (SI) system that prevents inbreeding and promotes outcrosses. SI is usually controlled by a single, multiallelic S-locus. In gametophytically controlled system, SI results when the S-allele of the pollen is matched by one of the two S-alleles in the style, while in the sporophytic system self-incompatible reaction occurs by the interaction between the pistil genotype and genotype of, not the pollen, but the pollen parent In the former system the self-incompatible phenotype of pollen is determined by the haploid genome of the pollen itself but in the latter the pollen phenotype is governed by the genotype of the pollen parent along with the occurrence of either to-dominant or dominant/recessive allelic interactions. In the sporophytic type the inhibition reaction occurs within minutes following pollen-stigma contact, the incompatible pollen grains usually failing to germinate, whereas in gametophytic system pollen tube inhibition takes place during growth in the transmitting tissue of the style. Recognition and rejection of self pollen are the result of interaction between the S-locus protein in the pistil and the pollen protein. In the gametophytic SI the S-associated glycoprotein which is similar to the fungal ribonuclease in structure and function are localized at the intercellular matrix in the transmitting tissue of the style, with the highest concentration in the collar of the stigma, while in the sporophytic SI deposit of abundant S-locus specific glycoprotein (SLSG).is detected in the cell wall of stigmatic papillae of the open flowers. In the gametophytic system S-gene is expressed mostly at the stigmatic collar the upper third of the style length and in the pollen after meiosis. On the other hand, in the sporophytic SI S-glycoprotein gene is expressed in the papillar cells of the stigma as well as in e sporophytic tape is cells of anther wall. Recognition and rejection of self pollen in the gametophytic type is the reaction between the ribonuclease in the transmitting tissue of the style and the protein in the cytoplasm of pollen tube, whereas in the sporophytic system the inhibition of selfed pollen is caused by the interaction between the Sycoprotein in the wall of stigmatic papillar cell and the tapetum-origin protein deposited on the outer wall of the pollen grain. The claim that the S-allele-associated proteins are involved in recognition and rejection of self pollen has been made merely based on indirect evidence. Recently it has been verified that inhibition of synthesis of S$_3$ protein in Petunia inflata plants of S$_2$S$_3$ genotype by the antisense S$_3$ gene resulted in failure of the transgenic plant to reject S$_3$ pollen and that expression of the transgenic encoding S$_3$ protein in the S$_1$S$_2$ genotype confers on the transgenic plant the ability to reject S$_3$ pollen. These finding Provide direct evidence that S-proteins control the s elf-incompatibility behavior of the pistil.

원형질배양과 체세포잡종

한창열 (Chang Yawl Harn) 한국식물학회 1972 Journal of Plant Biology Vol.15 No.3

This report deals with lead and zinc contamination of roadside soil and plants caused by motor vehicles as a function of distance from the road edge. The concentrations of Pb and Zn in roadside soil and plant samples from several locations decrease regularly with increasing distance from traffic. Soil samples up to 24m distance from the road edge are contaminated with more than 12.99 ppm lead, and 13.40 ppm zinc. The decrease in Pb and Zn contamination with increasing distance from the road is characteristically curvilinear; the relative coefficiency of Pb and Zn with distance is -0.69, -0.48, respectively. The average contents of Pb and Zn in plants are 21.5 ppm and 30.00 ppm. It is suggested that the contamination is related to the composition of gasoline, motor oil and to roadside of the residues of this metals.

개나리속의 종간교잡에 (種間交雜) 관한 연구

한창열 (Chang Yawl Harn) 한국식물학회 1961 Journal of Plant Biology Vol.4 No.1

Harn, Chang Yawl (Chonpuk U., Iri, Korea)-Studies on the interspecific crossing of FORSYTHIA Genus. Kor. Jour. Bot. 4 (1) 1~8 196l: Interspecific crossing of two species, F. saxatilis and F. Koreana, was caerried out in order to make clear the segregatifn ratio of style length, mode of fertility, the fertility of Fl generratio, dioecism, and other taxonomic question, the result of which being summarized as follows: 1) Style length is segregated inte 1:1 ratio. 2) The behavior of fertility in the legitimate and illegitimate unions between the different species is exactly like that in the two dimorphic forms of the same species. 3) The mode of fertility between the long and short style of the Fl generation also follows that of the heterostyle plants. 4) No difficulties or irregularities are observed in the interspecific crossing and the Fl`s ferility. 5) In Fl generation exceedingly high morphological and physiological variations are observed. 6) The short style individual is well fertilized and sets seed when legitimately combined. The insistence that the short style is male, this genus being dioecious, is groundless. 7) Among Fl individuals, are observed a few dwarf-types with tiny and weak vegetative and reproductive organs: 8) The two species used behave in many ways like the different styles of the same species.

Zebrina pendula 의 웅성배우체에 (雄性配偶體) 관한 연구

한창열(Chang Yawl Harn),이병기(Byung Ki Lee) 한국식물학회 1962 Journal of Plant Biology Vol.5 No.2

HARN, Chang Yawl & Byung Ki LEE (Chunpuk National Univ.) Studies on the microgametophyte of Zebrina pendula. Kor. Jour. Bot. V(2): 1-5. 1962. Attempts were made in order to make clear the meiotic irregularities, male gametophyte formation and spermatogenesis for the purpose of applying these embryological facts to taxonomic works. Followings are the results obtained: 1. Meiosis is extremely irregular, giving rise to giant, micro, and empty pollens. Meiotic division in each PMC is observed synchronizing. 2. In the microspore immediately before the vegetative-generative nuclear division, the nucleus locates appressed to the inner wall, most of the other part of the cell being occupied by large vacuoles. 3. Spindle symmetry in the microspore nuclear division is of intermediate type. 4. Nuclear divisions in each microspore are not synchronized as in meiosis. 5. Generative nucleus is located in close contact with the inner wall, while the vegetative nucleus occupies the central part of pollen grain. The pollen in this stage looks somewhat like broad bean. Afterward the generative nucleus loses contact with the wall, the nuclear shape changing from lenticular to roundsh. 6. The generative nucleus in fully matured pollen grain usually takes the shape of crescence, those in abortive pollen, lenticular or ellipsoidal, etc.