Membrane Microarray를 이용한 Resveratrol에 의해 차별적으로 발현되는 유전자 군의 분석

김종식,장민정,김효은,김순영,김병오,손호용,Kim, Jong-Sik,Jang, Min-Jung,Kim, Hyo-Eun,Kim, Soon-Young,Kim, Byung-Oh,Sohn, Ho-Yong 한국생명과학회 2007 생명과학회지 Vol.17 No.8

In the present study, we investigated whether several phytochemicals (resveratrol, genistein, epicatechin gallate, dially disulfide, caffeic acid phenetyl ester) and sulindac sulfide could induce expression of tumor suppressor p53 protein in human colorectal HCT116 cells. We found that p53 was dramatically induced by all phytochemical treatments except sulindac sulfide. Among treated phytochemicals, we selected resveratrol for further experiments because it is one of the highest p53 inducer. Using a Western blot analysis, we found that resveratrol induced p53 in a dose- and time-dependent manner. Additionally, using membrane-based microarray analysis, we found that twenty-five genes were up-regulated and two genes were down-regulated by resveratrol treatment. Among the up-regulated genes, we selected 4 genes and performed reverse-transcription-PCR to confirm microarray data. The results of RT-PCR were highly accorded with those of membrane microarray. In addition, we found that thrombospondin-1 (TSP-1) expression was not dependent on p53 presence, whereas mammary serine protease inhibitor (MASPIN) expression was dependent on p53 expressed by resveratrol treatment. The results of this study may help to promote our understandings of the molecular mechanisms of chemoprevention that are mediated by resveratrol in human colorectal cancer.

탐방-뉴질랜드 루트번트랙에서

김종식,Kim, Jong-Sik 한국자연공원협회 1997 한국자연공원 Vol.66 No.-

동북아 기업발전포럼 참관기 (2016)

김종식,Kim, Jong-Sik 고려인삼학회 2016 고려인삼연구와 산업 Vol.10 No.2

롤편심을 포함한 냉간압연 시스템의 다변수 제어

김종식,김승수,Kim, Jong-Sik,Kim, Seung-Su 대한기계학회 1997 대한기계학회논문집 Vol.21 No.3

A disturbance rejection controller using eccentricity filtering and LQ control techniques is proposed to alleviate the effecto of major roll eccentricity in multivariable cold-rolling processes. Fundamental problems in multivariable cold-rolling processes such as process time delay inherent in exit thickness measurement and non-stationary characteristics of roll eccentricity signals can be overcome by the proposed control method. The filtered instantaneous estimate of roll eccentricity may be exploited to improve instantaneous estimate of the exit thickness variation based on roll force and roll gap measurements, and a feedforward compensator is augmented as a reference for a gaugemeter thickness estimator. LQ feedback controller is combined with eccentricity filter for the attenuation of the exit thickness variation due to the entry thickness variation. The simulation results show that the roll eccentricity disturbance is significantly eliminated and other disturbances also are attenuated.

'91중국국제과학기술학술회의 참가기

김종식,Kim, Jong-Sik 한국과학기술단체총연합회 1991 과학과 기술 Vol.24 No.11

재발한 Vulvar 종양의 근접치료 시 Modified Mupit Applicator의 적용

김종식,정천영,오동균,송기원,박영환,Kim, Jong-Sik,Jung, Chun-Young,Oh, Dong-Gyoon,Song, Ki-Won,Park, Young-Hwan 대한방사선치료학회 2006 대한방사선치료학회지 Vol.18 No.1

Purpose: To evaluate whether modified MUPIT applicator can effectively eradicate recurrent tumor in uterine cervix cancer and reduce rectal complication after complete radiation treatment. Materials and Methods: Modified MUPIT applicator basically consists of an acrylic cylinder with flexible brain applicator, an acrylic template with a predrilled array of holes that serve as guides for interstitial needles and interstitial needles. CT scan was peformed to determine tumor volume and the position of interstitial needles. Modified MUPIT applicator was applied to patient in operation room and the accuracy for position of interstitial needles in tumor volume was confirmed by CTscan. Brachytherapy was delivered using modified MUPIT applicator and RALS(192-lr HDR) after calculated computer planning by orthogonal film. The daily dose was 600cGy and the total dose was delivered 3,000 cGy in tumor volume by BID. Rectal dose was measured by TLD at 5 points so that evaluated the risk of rectal complication. Results: The application of modified MUPIT applicator improved dramatically dose distributions in tumor volume and follow-up of 3 month for this patient was clinically partial response without normal tissue complication, Rectal dose was measured 34.1 cGy, 57.1 cGy, 103.8 cGy, 162.7 cGy, 165.7 cGy at each points, especially the rectal dose including previous EBRT and ICR was 34.1 cGy, 57.1 cGy. Conclusion: Patients with locally recurrent tumor in uterine cervix cancel treated with modified MUPIT applicator can expect reasonable rates of local control. The advantages of the system are the fixed geometry provided by the template and cylinders. and improved dose distributions in irregular tumor volume without rectal complication.

시론-수학자가 이룬 노력의 산물 잊지말자

김종식,Kim, Jong-Sik 한국과학기술단체총연합회 1989 과학과 기술 Vol.22 No.7

모바일 표준화 전략

김종식,Kim, Jong-Sik 한국정보통신기술협회 2006 TTA저널 Vol.108 No.-

롤편심을 포함한 냉간압연 시스템의 다변수 제어

김종식,김승수,Kim, Jong-Sik,Kim, Seung-Su 대한기계학회 1997 大韓機械學會論文集A Vol.21 No.3

A disturbance rejection controller using eccentricity filtering and LQ control techniques is proposed to alleviate the effecto of major roll eccentricity in multivariable cold-rolling processes. Fundamental problems in multivariable cold-rolling processes such as process time delay inherent in exit thickness measurement and non-stationary characteristics of roll eccentricity signals can be overcome by the proposed control method. The filtered instantaneous estimate of roll eccentricity may be exploited to improve instantaneous estimate of the exit thickness variation based on roll force and roll gap measurements, and a feedforward compensator is augmented as a reference for a gaugemeter thickness estimator. LQ feedback controller is combined with eccentricity filter for the attenuation of the exit thickness variation due to the entry thickness variation. The simulation results show that the roll eccentricity disturbance is significantly eliminated and other disturbances also are attenuated.

분절화된 인간 배아에서 세포자연사와 Fas, Fas-ligand, Bax, Bcl-2 발현에 관한 연구

김종식,김명신,양현원,류재혁,윤용달,배인하,정병준,송현진,Kim, Jong-Sik,Kim, Myoung-Shin,Yang, Hyun-Won,Yu, Chai-Hyeock,Yoon, Yong-Dal,Bae, In-Ha,Jung, Byeong-Jun,Song, Hyun-Jin 대한생식의학회 2002 Clinical and Experimental Reproductive Medicine Vol.29 No.3

Objective : The present study was performed to investigate whether apoptosis occur in human embryos by annexin staining and detect the expression of Fas, Fas-ligand (FasL), Bax, and Bcl-2 in human fragmented embryos derived from IVF-ET by immunofluorescence and Western blot analysis. Materials and Methods: Using annexin staining, immunofluorescence and Western blot analysis on normal and fragmented embryos, we were able to detect apoptotsis and apoptotic gene products in fragmented embryos. Result: Phosphatidylserine (PS) translocation, the marker for apoptosis, were detected frequently in fragmented embryos. Bcl-2 and Bax protein were detected in both fragmented and non-fragmented embryos. When fragmented embryos compared to normal embryos, immunofluorescent intensity of Bcl-2 tended to be lower in fragmented embryos. Bax gene expression increased in the fragmented embryos compared to the normal embryos. This result supports a model in which the molar ratio of Bcl-2 to Bax determines whether apoptosis induced or inhibited in human embryo. Fas was highly expressed in human preimplantation embryos but not FasL. It suggests that embryo may undergo apoptosis by binding with FasL produced by follicular or immune cells. Conclusion: The over expression of Bax and Fas will trigger apoptosis to lead embryo fragmentation and change embryo to be nonviable.