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金映來 忠南大學校 1968 論文集 Vol.7 No.-
Genetical studies in respect of self-compatibility and incompatibility in the progenies of the selfed plants, seasonal and temperature effects on self-compatibility, and identification of genoty pes of self-comapatible lines in respect to anthocyanin coloration by the mode of F2 segregation in the chinese cabbage Brassica pekinensis Rupr. were conducted during 1959 to 1967. 1. Results obtained with regard to compatibility reaction in these experiments were much complicated to be fully explained on the base of any known genetical mechanism. Bateson (1951) and Murakmi's (1965) hypothesis appeared to be somewhat similarity. Based on their hypothesis, the author proposes modified and new hypothesis are as follow : 1) The multiple factor designated at the T_1T_2, non allelic to oppositional factor S, but modifying the S locus was postulated. The T_1T_2 alleles and the S alleles in S locus interacted to change the compatibility reaction of the pollen from self-incompatibility to self-compatibility. These multiple factors have also an additive effects in certain combination with homozygous S alleles, e.f., a plant with S_4S_4T_1T_1T_2T_2(abbreviated as T_1T_2) genotype is self-incompatible but S_2S_2T_1T_1genotype plant is self-compatible. Moreover, dementional traits are more sensitive to environmental modification, since the percentage of self-fertility depend on temperature and which cause continuous variations on self-fertility in low temperature. Pollen with recessive homozygous t_1t_2 alleles seems to be ineffective in self-fertilization. 2) Oppositional factor S alleles in var. Kyoung-Do had S_1 to S_4 alleles, and dominant relationships of the different S alleles in the pistil and the pollen are determined as shown in the Table 11. 3) In the resiprocal cross H(S_2S_2T_1T_2) (High self-fertility×Low self-fertility), the H(S_2S_2T_1T_2) female parent was emasculated and crossed was higher in self-hertility than L(S_2S_2T_1T_2) parent was emasculated and cross was made by H(S_2S_2T_1T_2) pellen. (Table 2). The data indicate the possibility of plasmagene influencing the expression of self-compatibility. Based on the above proposed hypothesis, the genotypes in respect toself-compatibility of all selfed plants and their progenies were postulated easily. (Table 12, Fig 1 & 4). 2. Seasonal and temperature effects on self-compatibility in the chinese cabbage were not known before. The present study indicate that compatibility reaction of self-compatible lines of this plant was temperature sensitive in seasonal conditions. At 21.2℃, 22.9℃ during flowering period in natural conditions, approximately 93% of selfed flowers were setting seeds. But at 18.4℃ temperature in green house conditions, 71% selfing seed were obtained. (table 4). The rate of self-fertility in compatible styles increase with a rise in temperature within the range employed in this experiment. There were highly significant differences among years in all self-generations for percentage of setting seed. The correlation between temperature and self-fertility was +0.955. (Table5) 3. Anthocyanin coloured lines in midrib segregated from self-fertilized pedigree of chinese cabbage was obtained in S_1 and subsequent generations of the crossing (Table 7,8, & 9) Anthocyanin colonation midrib of this plant was considered to be due to the basic genes responsible for the production of the pigment, C and A.
십자화과 식물의 나출원형질체의 단리와 그 배양에 관한 연구
金映來,李英馥,咸麟基,朴敎善 충남대학교 농업과학연구소 1986 農業技術硏究報告 Vol.13 No.2
The study was carried out to identify several factors affecting isolation and culture of cotyledone and leaf mesophyll protoplasts of cabbage (Brassica oleraceae), petsai (B. campestris subsp. pekinensis) and rape (B. napus). High viable protoplasts could be obtained when the cotyledon and the leaf mesophyll tissue of all species were treated with enzyme solution composed of 1% macerozyme 'R-10', 1.5% Onozuka 'R-10', 10 %mannitol and 50,0mM CaCl_22H_2O for 4 hours. The protoplasts which obtained from the cotyledon of all species except the cabbage and the leaf mesophyll tissue of all species were divided on NN culture medium supplemented with 9.1% mannitol, 1% glucose, 1% sucrose, 1 mg/ℓ 2,4 -D, 0.5mg/ℓ NAA and 0.5mg/ℓ BA. The division of the rape leaf mesophyll protoplasts were continued and led to colony.
KIM, YOUNG RAE 忠南大學校 1959 論文集 Vol.1 No.-
1. Study was conducted to induce self fertile mutants in Brassica Peckinensis Rupr. 2. Germinating seeds of three different Varieties of Chinese cabbage cultivated in Korea Kl(Kyoung-To), Nl(Nosaki), Cl(Chung-Bang) were treated with three different dosages of X ray(1,000r, 2,000r and 5,000r). 3. In this preliminary test, X raying of seed with a dosage of 5,000r resulted only in following visible effects on seedlings. a)slight mottling of first three leaves, and b)plant height slightly below that of control. 4. One morphological change was observed which has three cotyledon plant. 5. All of the surving seedlings were transplanted to the field, and did not show any visible effects of irradiation up to the time of following. 6. There were no different sensibility to X ray treatment among the three varieties of Chinese cabbage.