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효모 유전자 발현용 (發現用) Promoter 개발에 관한 연구
정동효,정호권,박준희,심상국 ( Dong Hyo Chung,Ho Kwon Chung,Joon Hee Park,Sang Kook Shim ) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.1
The purpose of this study was the development of promoter for the lacZ` gene. Two heterologous promoter Ⅰ and Ⅱ of lacZ` gene were isolated from chromosomal DNA Bam HI fragment of yeast. The size of the promoter Ⅰ was estimated to be 2.5 kb and β-galacrosidase activity was 124.6 U/㎎ protein, and the size of the promoter Ⅱ was 4.0 kb and its β-galactosidase activity was 168.8 U/㎎ protein, respectively. The stability of the recombinant YEp plasmid in the transformant was from 52.7 to 67.4% at minimal medium. YIp plasmid was constructed from YEp plasmid, and expressed both in E. coli and yeast. The promoter Ⅰ aid Ⅱ iso-laced from yeast chromosomal DNA can be used for promoter of plasmid YEp and YIp.
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온도조절형 발현 Vector 를 함유한 Phenylalanine 생산균의 분자육종
정동효(Dong Hyo Chung),심상국(Sang Kook Shim),이영춘(Young Chun Lee),정호권(Ho Kwon Chung) 한국응용생명화학회 1995 Applied Biological Chemistry (Appl Biol Chem) Vol.38 No.1
In order to produce phenylalanine without tyrosine co-production, we constructed various temperature-controllable expression vectors by insertion of lower expression of the tyrA gene into the plasmid pSY130-14. And tyrosine revertant to cultivate without addition of tyrosine, was selected from Escherichia coli strain AT2471[tyrA , thi ] by spontaneous mutation. The strain AT2471 harbouring plasmid pSY146A and the tyrosine revertant 5 harbouring plasmid pSY111-14 produced 12 g/ℓ and 15 g/ℓ of phenylalanine respectively in a 2.5ℓ jar fermenter at a constant temperature of 39℃ after 55 hours cultivation.
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一株의 乳酸菌(L. bulgaricus)의 耐性에 關하여
鄭鎬權,朴麟鍾 건국대학교 1971 學術誌 Vol.12 No.1
1.A highly acid productive strain of lactic acid bacteria which was isolated from Kon-kuk milk plant, was identifid as Lactobacillus bulgaricus. And it was thought to be very useful in milk fermentation. 2.Strong antagonism to the pathgongenic Enterobacteria was found in fermented milk-solution of L. bulgaricus, and the solution was more effective on the two vibrio strains. 3.Antibaterial substance in fermented milk of L. bulgaricus was water soluble and thermostable. And the activity was strong in acidic solution (pH 4.0) and feeble in the neutralized (pH 7.2). 4.During 20 days of preservation (at 25'c), very little increments of lactic acid were observed in fermented milk solution which contains more than 50ppm of D.H.A. But the living cell of lactic acid bacteria was detected in all cases. 5.Active cell of the bacteria was contiually found for 40 days in the formented milk solution which contains 10 ppm. of DHA. while in controlled case for 35 days. And it was also detected in the solution added 50ppm. of D.H.A. after 30 days preservation.
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