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Joint combining ability and regression analysis for the study of genotype environment interactions
Seung Keun Jong(鄭丞根) 한국육종학회 1981 한국육종학회지 Vol.13 No.3
Statistical procedures to analyse joint combining ability and regression analysis are presented following Griffing (1956), Singh (1974) and Lin et al. (1977). Procedures of calculating sums of squares for Method I, II, III and IV and analysis of variance tables are also provided. A numerical example of analysis-IV is also used to show the advantages of this method in selecting parents for better performance with high stability.
Seung Keun Jong(鄭丞根),Hyeon Gui Moon(文賢貴),Bong Ho Choe(崔鳳鎬),Keun Yong Park(朴根龍) 한국육종학회 1986 한국육종학회지 Vol.18 No.1
Black streaked dwarf virus(BSDV) is one of the most important diseases of corn(Zea nays L.) in Korea. Dissemination of new high yielding corn hybrids is limited to the southern areas due to only this epidemic disease. There are no known published reports concerning the inheritance of reaction to BSDV in corn. This study was conducted to select resistant genotypes to BSDV and elucidate the genetic control of resistance to BSDV. Total of 965 local collections, introduced inbreds and hybrids were screened under field conditions in 1980 and 1981, 8-entry diallel crosses were tested in 1980 and generation mean analysis of parental, F₁, F₂ and backcross populations from the crosses of 5 resistant and susceptible lines were made under field conditions in 1981. Nine Korean local collections, KL-2, KL-10, KL-11, K79004, K79091, K79111, K79123 and K79124, and one introduced inbred, Ga 209, were selected as resistant genotypes to BSDV. Only specific combining ability was appeared to be significant in determination of BSDV resistance in the diallel analysis, indicating the relative importance of nonadditive genes for BSDV resistance. Crosses between resistant local collections and susceptible introduced inbreds such as KS15×KL-7, KS15×KL-1, KL-4×KS15 and KL-5×A635 showed high specific combining ability effects to increase resistance to BSDV in their F₁s. Generation mean analysis also indicated that dominance effect was more important than additive gene effect in inheritance of resistance to BSDV in corn, although the relative importance varied with the populations. Significant heterosis effect was also detected between resistant and susceptible lines. This study suggested that BSDV reaction in tested lines was conditioned by primarily nonadditive genes and breeding for resistant to this disease should be effectively accomplished by reciprocal recurrent selection procedure.
수도의 건물 생산 및 배분의 수리적연구 II. 이앙기에 따른 부위별 건물배분
조동삼,정승근,허훈,육창수,Cho, Dong-Sam,Jong, Seung-Keun,Heo, Hoon,Yuk, Chang-Soo 한국작물학회 1990 한국작물학회지 Vol.35 No.3
Two rice varieties, Samkangbyeo and Sangpungbyeo, were transplanted on 1/2000a pots at 6 different dates beginning on May 11 with 10 day interval in 1987 and at 4 different dates beginning on May 21 with 10 day interval in a paddy field at the Chungbuk Provincial Rural Development Administration. Dry matter distributions to stem and leaf sheath, leaves and ear at different growth stages were analyzed to provide basic informations neccessary for the development of dynamic growth model. Dry matter production was reduced as transplanting was delayed and the degree of reduction was greater at the transplanting later than June 1. Dry matter distribution to stem and leaf sheath was increased up to 60-70 days after transplanting with the maximum ratio between 60-70%, which were decreased to 37-43% in pots and 27-33% in field at the end of ripening stage. On the other hand, dry matter distribution to leaf blade was decreased from 40-50% at transplanting to 11-17% at harvesting. Ear dry matter distribution increased rapidly after heading and the distribution ratio was 42-49% in pots and 52-62% in field. Although regression equations to predict dry matter distribution to different parts of rice plant were satisfactory for individual experiment, the application to different experiment was not appropriate.
수도의 건물생산 및 배분의 수리적 연구 III. 재식밀도에 따른 부위별 건물배분
조동삼,정승근,허훈,육창수,Cho, Dong-Sam,Jong, Seung-Keun,Heo, Hoon,Yuk, Chang-Soo 한국작물학회 1990 한국작물학회지 Vol.35 No.4
In developing dynamic growth model of a crop, it is important to estimate accurate dry matter partition to different parts of crop plants. Two rice varieties, Samkang and Chucheong, were transnplanted with three planting densities of 72. 90 and 120 hills per 3.3㎡ on May 30 and June 15 in 1988 to study the effect of planting density on dry matter partition in rice plants. Total dry wight per square meter of two varieteis in May 30 transplanting were greater than those in June 15 transplanting. Total dry wights were increased as planting density was increased. The response of dry weights of differents parts of rice plants per hill were decreased as the density was increased. Although the difference in dry weights of leaf blade and stem and sheath between two varieties was not great, greater ear weight of Samkang resulted in greater total dry weight than that of Chucheong. Despite of transplant in date and planting density on dry weights, the ratio of dry matter partition to different parts of rice plants at a certain growth stage remained constant. Estimated dry weights of different parts at two stages of growth based on average ratio of dry matter partition over two transplantion dates and planting densities agreed well with those observed.
우선희,김홍식,송범헌,이철원,박영목,정승근,조용구,Woo, Sun-Hee,Kim, Hong-Sig,Song, Berm-Heun,Lee, Chul-Won,Park, Young-Mok,Jong, Seung-Keun,Cho, Yong-Gu 한국식물생명공학회 2003 식물생명공학회지 Vol.30 No.3
In this review, we described the catalogues of the rice proteome which were constructed in our program, and functional characterization of some of these proteins was discussed. Mass-spectrometry is the most prevalent technique to rapidly identify a large number of proteome analysis. However, the conventional Western blotting/sequencing technique has been used in many laboratories. As a first step to efficiently construct protein cata-file in proteome analysis of major cereals, we have analyzed the N-terminal sequences of 100 rice embryo proteins and 70 wheat spike proteins separated by two-dimensional electrophoresis. Edman degradation revealed the N-terminal peptide sequences of only 31 rice proteins and 47 wheat proteins, suggesting that the rest of separated protein sports are N-terminally blocked. To efficiently determine the internal sequence of blocked proteins, we have developed a modified Cleveland peptide mapping method. Using this above method, the internal sequences of all blocked rice proteins(i, e., 69 proteins) were determined. Among these 100 rice proteins, thirty were proteins for which homologous sequence in the rice genome database could be identified. However, the rest of the proteins lacked homologous proteins. This appears to be consistent with the fact that about 45% of total rice cDNA have been deposited in the EMBL database. Also, the major proteins involved in the growth and development of rice can be identified using the proteome approach. Some of these proteins, including a calcium-binding protein that tuned out to be calreticulin, gibberellin-binding protein, which is ribulose-1.5-bisphosphate carboxylase/oxygense active in rice, and leginsulin-binding protein in soybean have functions in the signal transduction pathway. Proteomics is well suited not only to determine interaction between pairs of proteins, but also to identify multisubunit complexes. Currently, a protein-protein interaction database for plant proteins(http://genome.c.kanazawa-u.ac.jp/Y2H)could be a very useful tool for the plant research community. Also, the information thus obtained from the plant proteome would be helpful in predicting the function of the unknown proteins and would be useful be in the plant molecular breeding.
In vitro culture of hybrid ovule between Fagopyrum esculentum Moench and F. homotropicum Ohnishi
Sun Hee Woo(禹仙熙),Taiji Adachi(足立泰二),Seung Keun Jong(鄭丞根) 한국육종학회 1997 한국육종학회지 Vol.29 No.3
Interspecific hybridization in buckwheat is of great importance to transfer desirable genes from wild species to cultivars as well as to broaden genetic variation in cultivars. In vitro ovule culture following the bud pollination was made to overcome postzygotic cross/self-incompatibility in interspecific hybridization between Fagopyrum esculentum Moench (common heterostylous buckwheat) and F. homotropicum Ohnishi (homostylous buckwheat). The percentage of fertilized ovaries of F. esculentum flowers (thrum-type) pollinated with F. homotropicum was 50%, while that of the reciprocal cross was as low as 10% and their ovaries were smaller. Vigorous pollen tube elongation following the bud pollination indicated the possibility for overcoming of breeding barriers in interspecific crosses between buckwheat species. Pre-embryo formation was observed 48 hours after pollination and ovules cultured up to 5 days after pollination tended to develop callus. But ovules isolated 11 days after pollination had apparently lost their viability. Hybrid plantlets were produced from only the ovules dissected 5 days after bud pollination. Regeneration rate was better in ovule culture on White medium than other mediums tested. Interspecific hybrid plants between F. esculentum and F. homotropicum were regenerated with the rate of 24.2% in average.