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면역조직화학과 In Situ Hybridization을 동시에 적용한 흰쥐 선하수체 Prolactin과 Prolactin mRNA의 감별
백상호,최완성 대한내분비학회 1987 Endocrinology and metabolism Vol.2 No.2
Immunohistochemistry and in situ hybridization were simultaneously employed at the same tissue section for identification of prolactin(PRL) and PRL mRNA. Pituitary glands from normal female rats were dissected out after perfusion of 3% neutral buffered paraformaldehyde through the ascending aorta. After rinsing in 30% surcrose containing 0.02% diethyl pyrocarbonate, frozen sections were cut in a cryostat at 10 um.For immunohistochemical staining anti-rPRL and biotinylated anti-rabbit IgG were used, and in situ hybridization was performed with ^35S-labeled PRL cDNA followed by autoradiographic process and counter-stain with cresyl violet. By this combination method, PRL appeared as brown-colored granules in the cytoplasm of lactotrophs and PRL mRNA were co-localized as dark silver grains at the same lactotrophs. Silver grains were also observed at the cells which revealed negative response to anti-rPRL.
오스템퍼드 구상흑연주철의 파괴특성에 미치는 Ni 의 영향에 관한 연구
백상호,김홍범,김창규,최창옥 ( Sang Ho Baek,Hong Beom Kim,Chang Kuy Kim,Chang Ock Choi ) 한국주조공학회 1994 한국주조공학회지 Vol.14 No.1
N/A The effect of Ni addition, on the mechanical properties and fracture characteristics of Mo-Cu and Mo-Ni-Cu alloyed ductile iron austenitized at 900℃ and austempering temperatures of 250℃, 300℃ and 350℃. The tensile strength, yield strength and hardness are decreased and elongation and impact value are increased in both Mo-Cu and Mo-Ni-Cu alloyed austempered ductile iron, with increased austempering temperature. According to the austempering temperature are increased, the amount of retained austenite are increased. Maximum value of fracture toughness is obtained at 350℃ austempering temperature at this condition, the amount of retained austenite came to 40% in Mo-Ni-Cu alloyed ADI and 34% in Mo-Cu alloyed ADI. The fracture surface of ADI which had represented high toughness are showed a quasi-cleavage pattern and a dimple pattern with micro void. Comparing the fracture characteristics of Mo-Cu alloyed ADI with that of Mo-Ni-Cu alloyed ADI, the latter was superior to the former.
백상호,홍승환,유수홍,손홍규,Baek, Sang Ho,Hong, Seung Hwan,Yoo, Su Hong,Sohn, Hong Gyoo 대한토목학회 2014 대한토목학회논문집 Vol.34 No.5
SAR 센서는 마이크로파를 이용한 능동센서로 기상조건에 상관없이 영상을 취득할 수 있다는 장점이 있어, 국토관리 및 재해 모니터링 등에 활발히 활용되고 있다. 주기적으로 취득되는 SAR 영상을 효과적으로 활용하기 위해서는 자동화된 영상 정합기법이 필요하지만 영상의 촬영 시간 및 기하에 따라 다른 양상의 기하조건을 가진 취득됨에 따라 충분한 정합 정확도를 기대하기 어렵다. 이에 본 연구에서는 기울기 속성을 추가한 MI (Mutual Information) 기법과 FMT (Fourier-Mellin Transform)기법, SIFT (Scale-Invariant Feature Transform) 기법을 임의의 변위와 회전 공차를 적용하고, 해상도를 변화시킨 TerraSAR-X 영상에 적용하여 그 결과를 비교하였다. 비교 결과, MI 기법의 경우엔 서로 상이한 기하에서 촬영된 영상에 적용하였을 때에도 일정 크기의 영상소가 다수 분포할 경우 0~2 픽셀 수준의 정확도를 지닐 수 있는 반면, FMT 기법의 경우에는 같은 사물에 대해서도 그 영상소 값이 서로 상이하여 정합 오차가 수십에서 수백 픽셀로 나타났다. 또한 SIFT 기법의 경우에도 영상 정합을 위한 공액점의 정확도가 0~17 % 수준으로 매우 낮아 서로 상이한 기하조건으로 취득된 SAR 영상에 적용이 어려울 것으로 나타났다. SAR satellite can acquire clear imagery regardless of weather and the images are widely used for land management, natural hazard monitoring and many other applications. Automatic image matching technique is necessary for management of a huge amount of SAR data. Nevertheless, it is difficult to assure the accuracy of image matching due to the difference of image-capturing attitude and time. In this paper, we compared performances of MI method, FMT method and SIFT method by applying arbitrary displacement and rotation to TerraSAR-X images and changing resolution of the images. As a result, when the features having specific intensity were distributed well in SAR imagery, MI method could assure 0~2 pixels accuracy even if the images were captured in different geometry. But the accuracy of FMT method was significantly poor for the images having different spatial resolutions and the error was represented by tens or hundreds pixels. Moreover, the ratio of corresponding matching points for SIFT method was only 0~17% and it was difficult for SIFT method to apply to SAR images captured in different geometry.
조석의 침투류에 의한 패류 유생의 착저(着底)효과에 관한 기초적 연구
백상호,박광재,박영제,전제천,조기채,김이운 한국패류학회 2009 The Korean Journal of Malacology Vol.25 No.2
It is a research that free floating larva of Ruditapes philippinarum in compliance with infiltration flow of tidal accumulation and bottom-clinging system in high density also, It is a precondition this for the continuous augmentation of Ruditapes philippinarum resources. So in flow field of tidal the free floating larva produced the infiltration water tank that can possibly bottom-clinging and experiment the water tank which uses free floating larva, It evaluates that the acceleration effect of free floating larva by infiltration in objective. As a result, 1) The experiment on free floating larva's bottom sediment grain diameter came to be high recording as bottom-clinging rate at the static water field and even biologically it selects bottom-clinging substrate it will be able to confirm. 2) About occurrence of infiltration flow field is in case of that: the drift of a current 10cm/s , bottom sediment grain 1.21mm infiltration flow 0.3cm/s increase of 3~5 times was confirmed. 3) From free floating larva of Ruditapes philippinarum the choice of bottom sediment grain diameter depends in the biological factor and form the flow field the bottom-clinging acceleration effect was controled over physical stable condition. 4) In case of density of Ruditapes philippinarum free floating larva of sea area, bottom sediment grain diameter, flow condition which are very cleanly in the research that possibly could conjecture the free floating larva's bottom-clinging rate. It is a research that free floating larva of Ruditapes philippinarum in compliance with infiltration flow of tidal accumulation and bottom-clinging system in high density also, It is a precondition this for the continuous augmentation of Ruditapes philippinarum resources. So in flow field of tidal the free floating larva produced the infiltration water tank that can possibly bottom-clinging and experiment the water tank which uses free floating larva, It evaluates that the acceleration effect of free floating larva by infiltration in objective. As a result, 1) The experiment on free floating larva's bottom sediment grain diameter came to be high recording as bottom-clinging rate at the static water field and even biologically it selects bottom-clinging substrate it will be able to confirm. 2) About occurrence of infiltration flow field is in case of that: the drift of a current 10cm/s , bottom sediment grain 1.21mm infiltration flow 0.3cm/s increase of 3~5 times was confirmed. 3) From free floating larva of Ruditapes philippinarum the choice of bottom sediment grain diameter depends in the biological factor and form the flow field the bottom-clinging acceleration effect was controled over physical stable condition. 4) In case of density of Ruditapes philippinarum free floating larva of sea area, bottom sediment grain diameter, flow condition which are very cleanly in the research that possibly could conjecture the free floating larva's bottom-clinging rate.