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RISS 인기검색어
- 검색키워드
- 저자 : 申壬雨 (검색결과 4 건)
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申壬雨 우석대학교 의과대학 1968 우석의대잡지 Vol.5 No.1
In the present study, the metabolism of phospholipids of developing chick embryo brain has been studied, being compared with the levels of free and esterified cholesterol. Fertilized hen's eggs of a White Leghorn strain were incubated in a humidified atmosphere at 38-39℃. Brain specimens from 14th incubation day to 2 weeks after the hatching chick were examined. 1. The levels of total phospholipids in the chick embryo brain of the 14th incubation day was about 19㎎ per g wet tissue and followed by a marked increase until the 18th incubation day(33㎎/g wet tissue), and dropped down at 20th incubation day. Again, the concentration of total phospholipids began to increase markedly at hatching (corresponding to 21th incubation day), and reached its maximal level (41㎎/g wet tissue) at the 10th post-hatching day. 2. The concentration of phosphatidylcholine was 3㎎ per g wet tissue at 14th incubation day and was followed by a gradual increase until the 10th post-hatching day. 3. The level of phosphatidylethanolamine maintained at the same level until the hatching. The time course changes in the concentration of phosphatidylethanolamine in the brain of post-natal chick showed a similar pattern to that of phosphatidylcholine. 4. The concentration of phosphatidylinositol was 2.6㎎ per g wet tissue at 14th incubation day and dropped slightly during the subsequent incubation period and showed a gradual increase and attained its peak 10 days after the birth. 5. The level of total cholesterol in the chick embryo brain of the 15th incubation day was 7.5㎎ per g wet tissue and followed by a gradual increase until the hatching and maintained at the same level at 7 days post-hatching period. The changes in the concentration of free cholesterol during development showed a almost same pattern to that of total cholesterol. The level of esterified cholesterol remained unchanged throughout at pre and post-natal periods. 6. Included in the discussion are the relationship between phosphatidylcholine concentration and cholesterol esterifying capacity.
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鷄眼組織의 Phosphatidylcholine에 關한 觀察
申壬雨 우석대학교 의과대학 1968 우석의대잡지 Vol.5 No.1
The metabolism of phosphatidylcholine of chick eye during the pre- and postnatal development has been studied. Lipid was extracted by the method of Folch et al. and fractionated by thin-layer chromatography as described by Skipski et al. Lipid phosphorus was determined by a combination and modification of the methods of Bartlett and of Chen. The results were as follows: The concentration of phosphatidylcholine of embryonic eye changed very little from 14th(0.4㎎ per g. wet tissue) to 18th(0.45㎎ per g. wet tissue) incubation day. However, at 20th incubation day the concentration of phosphatidylcholine of eye began to increase remarkably and attained its peak at hatching(0.7㎎, 1.3㎎ and 1.6㎎ per g. wet tissue respectively for 20th, 21th incubation day and at hatching). After the hatching, the level of phosphatidylcholine was followed by a steady and gradual decrease and to adult level(0.5㎎ per g. wet tissue) was attained at 1 month after hatching.
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