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권무길,김득수,이정환,박상원,추영국,한연수,김주성,황경아,고기남,고기성 한국곤충학회 2012 Entomological Research Vol.42 No.1
Silkworm pupae have much potential and many applications as a natural medicine to promote human health. However, their chemical components have not been fully characterized or understood. HPLC analysis was conducted to determine the content ratio (%) of individual amino acids in total protein of the pupae. It showed that glutamic acid (18.3%), histidine (14.6%) and alanine (10.2%) are the most common amino acids in silkworm pupae. Fatty acid composition of silkworm pupae oil was revealed by high-pressure liquid chromatography and gas chromatography – mass spectroscopy analyses. They contain a high ratio of essential fatty acids, [a-linolenic acid (w-3 fatty acid] + linoleic acid) (49.0%), and also contain non-essential fatty acids, oleic acid (19.9%), palmitoleic acid (2.5%), palmitic acid (19.7%), stearic acid (8.6%), and eicosapentaenoic acid (EPA) (0.3%). In addition, they also contain antioxidants, quercetin diglucoside and nutritionally important riboflavin (vitamin B2). This study suggests that silkworm pupae are a nutritionally valuable food product and are applicable as cosmetic components with essential amino acids, essential fatty acids, antioxidants and vitamins. The animal experiment showed that alcohol dehydrogenase (ADH) activity was significantly higher in the liver of mice orally administered with 0.5 mg/mL of silkworm extract and alcohol than with commercial Dawn808™ and alcohol, indicating that silkworm pupae extracts have alcohol detoxification activity. Key words: quercetin diglucoside, silkworm pupae, vitamin B2, a-
권무일 대한마취과학회 1997 Korean Journal of Anesthesiology Vol.33 No.1
Background : 42 anesthesia-related medico-legal cases, consulted to the Korean Society of Anesthesiologists(KSA) in a recent 2 year period(1994, 11∼1996, 10) were analysed. Method : Results of the analysis were classified into 11 items. Results : Cases sources were 26 cases from civil court, 2 cases from criminal court, 8 cases from police stations and 6 cases from health centers, and involved surgical departments were obstetric & gynecology (17 cases), general surgery(7), orthopedic surgery(6), and others(12). Operation classification were Cesarean section(14 cases), reduction and fixations(5), gastrectomy(4) and others(19), and anesthesia methods were general anesthesia(36 cases), regional anesthesia(3), and local anesthesia(3). Involved hospitals were university hospitals(15), general hospitals(16) and private clinics(11), and involved anesthesiologists(anesthetists) were certified anesthesiologists(34), nurse anesthetists(3), and others(5). Patient's ages were classified into 0∼20 years old(4), 20∼40 ys old(23), 40∼60 years old(13) and over 60 years old(2), and patient's sex ratio was 16(male) to 26(female). Outcome of victims were deaths(26 cases), severe brain damages(10) and nerve injuries(6), and autopsy findings were hypoxic brain edema(4 cases), coronary artery diseases(3), and other findings(4). Causes of medico-legal problems were hypoxemia by ventilatory failure (12), pre-existing diseases(4), embolisms followed by operation(2), malignant hyperthermia(1), and uncertain cases(21). Conclusion : The most common cause of medico-legal problems was hypoxemia by ventilatory failure. (Korean J Anesthesiol 1997; 33: 154∼161)
Enhanced activities of reproductive system in male rat treated with male silkworm pupae extract
권무길,김득수,이정환,박세라,안준식,김주성,고기남,박성수,강영선,추영국,고기성 한국곤충학회 2013 Entomological Research Vol.43 No.2
A male silkworm pupae extract with herbal mixtures such as Rubus coreanus Miquel, Chinese matrimony vine Acanthopanax senticosus and tocopherol can be effectively used to recover or boost stamina. In this study, the effect of the male silkworm pupae extract on the reproductive system of Sprague–Dawley male rats was investigated. No clinical symptoms, and no dying or dead animals were found among experimental male silkworm larvae extract, male silkworm pupae extract and control rat groups during the study. No significant differences were found in body weights, feed or water consumption, or macroscopic examination among the three groups. No lesion was found upon necropsy. However, sperm in the group treated with male silkworm pupae extract were significantly more active than sperm in the control group. Sperm counts in both male groups treated with male silkworm larvae extract and male silkworm pupae extract were significantly higher than sperm counts in the control group. Analysis of phospholipid–hydroperoxide glutathione peroxidase (PHGPx) gene expression showed that the male silkworm pupae extract increased expression in testes by 26.7%. This study shows that the male silkworm pupae extract has potential to be used to enhance the function of the human reproductive system.