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박중곤,Hamayun Khan 한국생물공학회 2006 Biotechnology and Bioprocess Engineering Vol.11 No.6
Molecularly imprinted polymeric microbeads (MIPMs) were prepared by the suspension and modified suspension polymerization methods using D-phenylalanine as the template, methacrylic acid as the functional monomer, ethylene glycol dimethacrylate as the cross-linker, toluene as the porogen, polyvinyl alcohol as the stabilizer, and sodium dodecyl sulfate as the surfactant. The addition of a surfactant to the conventional suspension polymerization mixture decreased the mean particle size of the MIPMs and increased the adsorption selectivity. For the modified suspension polymerization method, the mean particle size of the MIPMs was smaller than the particle size of MIPMs prepared via conventional suspension polymerization. Moreover, the adsorption selectivity improved considerably compared to the adsorption selectivities of MIPs reported previously.
박중곤,김준균 한국화학공학회 1994 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.32 No.2
새로이 개발된 전기영동조에서 완충용액 대신 혼합유기용매를 매질로 사용하였고 단백질용액은 분자수준의 미수계로 제조하여 전극양단에 7 KV의 전압을 가하여 단백질의 이동을 시도하였다. 본 연구에서는 유기용매의 전도도를 측정하고 안정된 미수계를 이룰 수 있는 protic 유기용매를 선정하였으며 분자수준 미수계 단백질용액의 용액조성과 온도에 따른 안정성도 조사하였다. 분자수준 미수계의 단백질은 고전압하에서 단백질분자의 미수계덩이상태로 이동하였고 단백질의 이동방향은 기존의 전기영동과는 반대방향이었으며, 이동속도는 약 100배 이상의 증가가 있었다. Mixed organic solvent was used as a medium instead of buffer solution in the new developed filter paper electrophoresis cell. The sample protein solution was prepared as the micro-aqueous system and the protein of the sample protein solution migrated at the high electrical voltage of 7 kilo volts applied between two electrodes. We could select usable protic organic solvents by measuring the electrical conductivities of organic solvents and checking the stability of the sample protein solution. The protein of the sample protein solution moved lump by lump. The direction of the protein movement was reverse and the migration speed was more than 100 times faster than that of conventional electrophoresis system.
Production of Bacterial Cellulose by Gluconacetobacter hansenii PJK Isolated from Rotten Apple
박중곤,Youn Hee Park,Jae Yong Jung 한국생물공학회 2003 Biotechnology and Bioprocess Engineering Vol.8 No.2
A cellulose-producing strain isolated from rotten apples was identified as Gluconacetobacter hansenii based on its physiological properties and the 16S rDNA complete sequencing method, and specifically named Gluconacetobacter hansenii PJK. The amount of bacterial cellulose (BC) produced by G. hansenii PJK in a shaking incubator was 1.5 times higher than that produced in a static culture. The addition of ethanol to the medium during cultivation enhanced the productivity of bacterial cellulose, plus the supplementation of 1% ethanol into the culture medium made the produced BC aggregate into a big lump and thus protected the bacterial-cellulose-producing G. hansenii PJK cells in the shear stress field from being converted into non-cellulose-producing (Cel-) mutants. Cells subcultured three times in a medium containing ethanol retained their ability to produce BC without any loss in the production yield.
Production of bacterial cellulose by a static cultivation using the waste from beer culture broth
박중곤,Jung Hwan Ha,Omer Shehzad,Salman Khan,Seung Yong Lee,Joon Won Park,Taous Khan 한국화학공학회 2008 Korean Journal of Chemical Engineering Vol.25 No.4
Bacterial cellulose (BC) was produced by using the waste from beer culture fermentation instead of a chemically defined medium. Static cultivation was superior to the shaking cultivation on the basis of the BC production. The amount of BC produced during 120 hrs of cultivation using the waste from beer fermentation broth (WBFB) by a static cultivation was 4.52 g/L on the dry weight basis and much higher than 0.45 g/L produced from a Buffered Schramm and Hestrin (BSH) chemically-defined medium. The addition of 1% industrial-grade glucose to WBCB increased the production of bacterial cellulose from 8.46 to 13.95 g/L after 336 hrs of cultivation. Water soluble oligosaccharide (WSOS), the by-product obtained during BC cultivation increased to 5.05 g/L at 192 hrs of cultivation and then decreased to 2.18 g/L at 336 hrs