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Improvement of Resistance to Late Blight in Hybrid Tomato
박덕환,Yan Zhang,김병섭 한국원예학회 2014 Horticulture, Environment, and Biotechnology Vol.55 No.2
Late blight is one of the major diseases in tomato cultivated both in the greenhouse and the field. In thiswork, we determined the resistance levels of all progeny lines (F1 to F3) and a new variety (NV) F1 to the late blightdisease fungus to evaluate these hybrid lines as potential sources of resistance. The experiments were carried out ingreenhouse conditions in a completely randomized block design with three replications. Fifteen out of 204 F2 plantsof cross A (AV107-4 × L3708) and 13 out of 203 F2 plants of cross B (Campari × 917 × L3708) were found to beresistant to late blight in combined data from greenhouse trials. In addition, 5 resistant F3 lines derived from each ofthe two crosses A and B were selected for further investigation. By screening the NV F1 hybrids obtained by pollinationof the F3 lines by 3 male-sterile cultivars (Wonung-1, 2, and 3), two stable resistant hybrids, Wonung-2 × A120 andWonung-3 × B23, were finally produced. An examination of the qualitative characters in the NV F1 hybrids indicatedimproved fruit weight and quality. Taken together, our screening results indicate that both of these hybrids are goodsources of resistance and have high-value horticultural characters, which can be used for tomato breeding programs.
박덕환,Shree Prasad Thapa,김원식,허장현,임춘근 한국식물병리학회 2010 Plant Pathology Journal Vol.26 No.3
We designed a sensitive and specific PCR-based method with enterobacterial repetitive intergenic consensus (ERIC) primer to detect Erwinia pyrifoliae, which cause shoot blight in Asian pear, from a mixed culture and infected plant materials. The primers specifically detected only E. pyrifoliae and showed no cross-reactivity with other bacterial phytopathogens.
박덕환,김점순,허장현,임춘근,JunMoCho,SoonWoKwon 한국식물병리학회 2003 Plant Pathology Journal Vol.19 No.1
From 1996 to 1999, potato-growing areas in Korea were surveyed for identification and distribution of potato scab pathogens. Potato scab was widely distributed in the mass cultivation areas, especially in Jeju island, southern areas of Chonnam and Gyounggi provinces, and the alpine area of Gangwon province. Jeju island was the most affected area by this disease. A total of 55 Streptomyces strains were isolated from potato scab lesions, among which 40 strains were pathogenic on progeny tubers. Among the pathogenic strains, 21 strains were identified as previously described S. scabies, 7 strains as S. turgidiscabies, and 5 strains as S. acidiscabies, while 7 strains were observed as having distinct phenotypic properties. These strains were classified into six distinct clusters based on phenotypic characteristics and selected representative strains for each cluster. S. scabies (S33) had grey spores in a spiral chain. Meanwhile, S. turgidiscabies (S27) had grey spores, S. acidiscabies (S71) had white spores, S. luridiscabiei (S63) had yellow-white spores, S. puniciscabiei (S77) had purple-red spores, and S. niveiscabiei (S78) had thin and compact white spores, all in a rectiflexuous chain. Pathogenicity was determined by the production of thaxtomin A and homologs of nec1 and ORFtnp genes. In TLC, representative strains S33, S27, S71, S63, S77, and S78 produced a yellow band that co-migrated with the authentic thaxtomin A. However, thaxtomin A was not detected in chloroform extracts from oatmeal broth culture and slice tuber tissue of S. luridiscabiei (S63) and S. puniciscabiei (S77) by HPLC analysis. In addition, no homologs of nec1 and ORFtnp genes in S. acidiscabies (S71), S. luridiscabiei (S63), S. puniciscabiei (S77), and S. niveiscabiei (S78) were detected by PCR and Southern hybridization analysis.
Erwinia carotovora subsp. carotora에 의한 고추냉이(와사비) 세균성 무름병
박덕환,서상태,최준근,임춘근 한국식물병리학회 1998 Plant Pathology Journal Vol.14 No.5
Occurrence of soft rots was observed on wasabi (Wasabia japonica Matsum) grown in Chuncheon and Pyengchang Kangwon province, Korea. The symptoms appeared on the wasabi root, which became mushy and black. This eventually resulted in wilting and death of the aboveground parts of the wasabi. The causal organism was isolated from the infected lesions and was identified as Erwinia carotovora subsp. carotovora based on the morphological, physiological and biochemical characteristics, and on the results of the Biolog program (Biolog Inc., U. S. A.). E. carotovora subsp. carotovora is the first described bacterium which causes bacterial soft rot on wasabi in Korea.