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      • 絨毛性 "고나도트로핀"의 免疫學的 硏究

        朴孃實 최신의학사 1968 最新醫學 Vol.11 No.12

        For the practical use of immunological pregnancy test (11.1-R.), 'and technical familiarization, semi-quantitative determinations of H.C.G. were performed by means of hemagglutination inhibition technique. 1) H.C.G. of relatively high purity (5,427 IU/mg) was used as antigen and high titer of antiserum was obtained even with relatively small amount of antigen through booster injections of antigen and complete Freund's adjuvant. 2) To prove the specificity of the obtained antiserum, weighing technique using i nmature rat ovary was applied, and it was found that the 0.1 ml of the obtained antiserum could neutralize the increasing effect of 200 IU of H.C.G. in immature ovarian weight. 3) Analysis of H.C.G. used in this work by Ouchterlony method showed only one clear precipitatin band between antigen and antiserum, indicating that used H.C.G. contained a negligible amount of other proteins with antigenicity. 4) The sensitivity of hemagglutination inhibition test in this study could detect 0.2 IU/ml of H.C.G. 5) The sensitivity of the hemagglutination inhibition reaction was so adjusted that more than 600 IU/1 of H. C. G. could be detectable, and urine to be examined was diluted I : 2 and I : 4 with the pH 6.4 phosphorus buffered saline. Therefore positive reactions obtained when the urine contained more than 1, 2002,400 IU/1 of H.C.G. and positive reactions were classified as pregnant cases. By doing so, the highest accuracy of the pregnancy test was obtained. A total of 523 cases, both pregnant and non-pregnant control, were analysed and it was disclosed that the over-all accuracy rate was 98.5 per cent for the 2 groups combined. It was particularly accurate in the non-pregnant control, the rate being 100 per cent, without a single case of false positive. 6) The' urine of patients with cervical cancer or proteinuria was not caused false positive reaction in this study. 7) The semi-quantitative determination of H.C.G. in the random-urine of 340 pregnant women at various gestational period revealed that H.C.G. has been sharply increased after 5th week of gestation and reached to the peak in 9th10th week, giving an average of 118, 000 IU/1(4, 000512, 000 IU/1), then gradually declined to reach a point of stabilization in 24th week and 18,00026, 000 IU/1 has been maintained until the end of pregnancy.

      • KCI등재후보
      • KCI등재

        자궁경암의 세포학적 검사 해설 및 10예 보고

        김석환(SH Kim),양실(YS Park) 대한산부인과학회 1962 Obstetrics & Gynecology Science Vol.5 No.2

        1) The present status of gynecological exfoliative cytology is briefiy reviewed and ten cases of cervical lesion on which cytologic examination was performed are presented. More extensive studies on the treatment of invasive cervical carcinoma should be done. But, what is more inportant, the detection of the pre-clinical stage of cervical carcinoma should be advocated so as to prevent invasive carcinoma. For the purpose of detecting pre-clinical carcinoma, cytologic examination which is highly accurate, particularly in squamous cell carcinoma (accuracy according to Brewer: 95%), should be used periodically, at least once a year. 2) Cytologic examination is useful and convenient to confirm malignancy with-in a few hours in comparison to paraffin preparation of the tissue. 3) Cytologic examination is mandatory to detect cervical carcinoma in incomplete vaginal atresia and cervical canal carcinoma, which is difficult to be noted by speculum examination. 4) In the case of a cervical lesion which seems to be cervical erosion in appearance and forms hard induration to the parametrium, cytologic examination is decisive in differentiating whether it is infiammatory or carcinomatous in nature.

      • 副腎皮質細胞巨大症을 隨伴한 先天梅毒의 一剖檢例

        池堤根,李尙國,崔奎淞,朴孃實,任元一 中央醫學社 1964 中央醫學 Vol.7 No.6

        Pathologic-anatomic and pathologic-histologic studies were made on one autopsy case of congenital syphilis which waas serologrcally confirmed in maternal and cord blood, being accompanied with adrenal cyomegalia. Microscopical findinfs are principally composed of diffuse visceral fibrosis and widespread extramedullary hemopoiesis. Visceral fibrosis was most severe in the liver and pancreas, although spleen, lungs, kidneys and adrenal glands are also involved. Hear shows a focal hematopoietic lesion. Thymus cintains many foci of Dubois abscess. Liver is characterized by extensive periportal and intralobular connective tissue increase and laso extensive hematopoietic foci. The fibrotic change is more marked in right lobe especially around ductus venusus. Giant cell transformation of hepatic cells is also prominent. Lungs are enlarged and uniformly firm. In teralveolar septa show diffuse connective tissue increase and mononuclear cell infiltration. spleen is enlarged and also shows connective tissue proloferatin and numerous macrophages in between the splenic sinusoids. Adrenal gands are slighty thickended in capsular structure and the connective tissue increase is focally extended inward between the cords of cells of the permanent cortex. The fetal portion of the cortex shows a widespread change in the size, shape and nuclear pattern of its constituent cells. The cells are polyhedral measuring up to 45μ in diameter, and structures resembling inclusions are present. Brain shows syphilitic leptomenimgitis.

      • KCI등재

        쌍태난산에 관하여 - 증례를 중심으로

        김선원(SO Gim),조석신(SS Cho),이원기(WK Lee),양실(YS Park) 대한산부인과학회 1967 Obstetrics & Gynecology Science Vol.10 No.8

        Twin Dystocia is a extremly rare complication of multiple pregnancy, and a situation that affords real dystocia in the life time of a busy obstetrician, since no one has any extensive experience in handling this problem. Because of its rarity and seriousness, a case of locked twin with survival of both infants by C-section is presented, and the various methods of managing this grave obstetric complications are briefly discussed with a review of the literature.

      • KCI등재

        Rh혈형부적합으로 인하여 고빌리루빈 혈치를 동반한 쌍태아 및 막상제대부착태반

        이원기(WK Lee),송용덕(YD Song),김선원(SO Gim),양실(YS Park) 대한산부인과학회 1967 Obstetrics & Gynecology Science Vol.10 No.7

        A case of twin combined with hyperbilirubinenia due to Rh in compatibility, and velamentous insertion of cord are presented. The sex and the weight of these babies were as follows; 2.5kg (female), 2.9kg(male). These two babies were relatively well ellapsed without exchange transfusion during admission although the biliubin levels were high, and discharged uneventfully.

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