[隨筆] 장미 유감

成貞洙 한국마케팅연구원 1976 마케팅 Vol.10 No.7

晋州地方의 主要氣候 要素에 關한 分析的 硏究

金仁湖,成正秀 慶尙大學校 1987 論文集 Vol.26 No.1

우리나라이 南部?岸 內陸에 위치하는 慶南 普州地方의 氣象觀測所(35?12N, 128?06E)인 中央氣象臺釜山支臺 普州測候所에 의하여 觀測된 主要氣候要素인 氣溫을 비롯한 雲量, 日照率, 一射量, 相對?度, 風速과 風向, 降水量 등에 관하여 그 觀測値를 調査分析하고 또한 平年値와를 考察하였다. 普州地方에서 1986年度의 主要氣候要素에 관한 月別의 分布를 보면 Table 과 같으며, 年平均氣溫은 약 12.2℃,最高氣溫과 最低氣溫의 平均은 각각 약 6.7℃를 나타냈고 특히 平均氣溫은 平年에 비하여 약 1.0℃가량 낮았으며, 例年에 비해 1月中에 零下를 나타내는 추위가 계속 되었음을 보였다. 한편 日最高氣溫 平年보다 다소 높은 分布였으나, 日最低氣溫은 반대로 낮은 분포였다. 雲量, 日照率과 日射量의 年中 및 月別分布는 거의 비슷하였고, 相對溫度와 風速의 경우는 例年에 비해 높게 나타났다. 年總降水量은 平年値보다 약 370mm 이상 적었고, 年總蒸發量은 예년과 거의 비슷한 分布를 보였다. 月別 降水量分布의 경우, 1,2,5와 11月중은 약 30~40mm내의 4,7과 8月중은 약 110~140mm 내외가 예년에 비해 적었으며 한 편 6과 9月에는 예년보다 약 50~90mm 정도가 많은 분포를 보였다. 各 氣候要素의 月中旬別 分布는 Fig.1에서 Fig.8과 같이 나타났음을 알 수 있다. 季節別에 따른 各 氣候要素의 分布를 보면, Table 2와 같으며, 平均氣溫의 경우 봄철과 가을철은 平年에 비해 약 1.1~1.4℃ 가량이 낮았고, 最高氣溫은 거의 비슷하였으나 最低氣溫의 平均은 역시 예년에 비하여 약 1.3~1.5℃ 가량이 가을과 겨울철에 낮게 나타났으며, 특히 겨울철에는 예년에 비해서 약-4.0℃가량 낮은 分布를 보였다. 雲量, 日照率과 日射量의 分布는 대체로 예년과 비슷하였으나 相對溫度와 風速은 다소 크게 나타났다. 降水量은 年總量이 약1200.1mm가량으로 예년에 비해 현저하게 적었으며, 특히 4.7과 8月중은 약110~140mm 내외가 平年値 보다 낮은 分布였으며, 한편 蒸發量의 경우는 예년과 거의 비슷한 分布를 나타냈음을 알 수 있었다. 끝으로 本 硏究의 遂行을 위하여 基礎資料의 수집과정에서 많은 도움을 주신 中央氣象臺 釜山支臺 普州測候所의 李 準炫 所長님과 南 大佑 先生님께 깊은 感謝를 드립니다. This study was carried out to esimate the principle climate elements whcih were of airtemperature , amount of cloud, percentage of possible sunshine, amount of insolation, relative temperature amount of insolation, relative humidity, wind speed and direction, amount of precepitation and evaporation in 1986(Jan. lst.~ Dec.31th.) of chinju district in South Korea. Almost all meteorological data were observed by the Jinju Meteorological Office in chinju, Gyeongnam Prevince. As the results of this anaytical study , monthly and seasonal distributions of the principal climate elements were shown as Table-1and 2, and decadal changes of each climatic elements were plotted as Fig. (1)~(8).

晋州地防의 實效濕度 分布에 關하여(1980~'86)

金仁湖,成正秀 慶尙大學校 1988 論文集 Vol.27 No.1

This study was carried out to estimate the distribution and change of the mean effective humidity(Heff) during seven years(Jan. 1980-Dec. 1986) in the district of Jinju, Gyeongnam province in Korea. The basic meteoroloical data of relative humidity used in order to caculate the effective humidity were observed by the Jinju Meterological Office (35° 12' N, 125° 06' E) in Jinju. The results of this study obtained were as follows : Annual mean effective humidity was about ?? The distribution under?? was mostly appeared for seven months (from November to May next year) in a year and the minimum was about ??in January. The recorded minimum mean effective humidity under ?? was appeared in the 1st pentan of 3rd dekad in April 1980 (??), the 2nd pentad of 3rd dekad in January 1984 (??), and the 2nd pentad of 1st dekad in February1984 (??). Seasonal mean effective humidity was about ??(in spring),?? (in summer), ?? (in autumn), and Heff=44.5% (in winter). The frequency of mean effective humidity under Heff=50.0% and Heff=40.0% in a year was about 46.3% and 99% respectively. The frequency under Heff=50.0% in five months(Jan., Feb., May, April, and Dec.) distributed about 20-27 days monthly. The climatic characteristics in the view point of humidity was apparently shown dry-air (??) from January to March all around the year.

氣候的 指數에 의한 嶺湖南地方의 土壤型 考察

金仁湖,成正秀,姜光鏞 慶尙大學校 기초과학연구소 1987 基礎科學硏究所報 Vol.3 No.-

This study was carried out to estimate the soil-type based on the climatic indices for 23 sites in the district of Yeong-Honam in Korea. The climatic indices of the rain Factor (R. F. ), N-S quotient, P-E index (precipitation effectiveness ratio), and T-E index(thermal efficiency ratio) were numerically caculated by the several meteorological data observed from 1931 to 1980. The results of this study revealed the average value of R. F.≒91.6, N-S quotient ≒287.3, P-E index≒47.4, and T-E index≒66.4. The climatic characters of this area were shown as B (humid) and/or C (subhumid) in the monsoon climatic zone (B' mesothermal) and the plant rearing was a type of forest and/or grassland. It seemed that the most of all soil-type such as the yellow, redish, and brown soil were widely distributed in this area.

慶南地方의 實效濕度 分布에 關하여 : 釜山, 蔚山 및 忠武地方, 1951∼80 Busan, Ulsan, and Chungmu, 1951∼'80

金仁湖,成正秀 慶尙大學校 기초과학연구소 1988 基礎科學硏究所報 Vol.4 No.-

This study was carried out to estimate the distribution and change of the mean effective humidity during thirty years(Jan. 1951-Dec. 1980) in Busans, Ulsan, and Chungmu in Gyeongman, Korea. The basic meteorological data of relative humidity(R.H.) used in order to calculate the effective humidity H_eff were observed by the Busan Meterological Office(35°06'N, 129°02'E. H=69.2m), Ulsan Met. Station(35°33'N, 129°19'E, H=31.5m), and Chungmu Met. Station(34°50'N, 128°26'E, H=32.2m) The results of this study obtained were as follows: Annual mean effective humidity was respectively about H_eff=4.37%(in Busan), H_eff=46.5%(in Ulsan), and H_eff=47.6%(in Chungmu) The mean effective humidity in spring were about H_eff=42.9%(in Busan),45.6%(in Ulsan). and 46.1%(in Chungmu), in summer were about H_eff=53.8%(in Busan), 53.6%(in Ulsan). and 55.6%(in Chungmu), in autumn were about H_eff=43.5%(in Busan),48.3%(in Ulsan). and 48.0%(in Chungmu), in winterwere about H_eff=34.6%(in Busan),38.7%(in Ulsan). and 40.6%(in Chungmu) respectively. The distributions of the appeared frequency day under H_eff=50.0% in a year were about 274 days(in Busan), 243 days(in Ulsan), and 243 days(in Chungmu) respectively. The climatic characteristics in the view point of humidity were apparently shown dry-air ; as following the effective humidity H_eff=31.7∼39.1%(in Jan., Feb., Nov., and Dec., in Busan), H_eff= 35.5-40.1%(in Jan., Fed., and Dec., in Ulsan), and H_eff=38.6-39.2%(in Jan., Feb., and Dec., in Chungmu) all around the year.

南海沿岸地方의 溫量指數 및 추위指數 分布의 硏究

金仁湖,成正秀 慶尙大學校 기초과학연구소 1986 基礎科學硏究所報 Vol.2 No.-

農作物을 비롯한 모든 植物의 生育에는 지속적인 필요 熱量의 공급이 요구됨으로 적당한 氣溫의 장기간 유지가 있어야 한다. 특히 生育에 필요한 온도는 生育 期間의 口平均氣溫의 總合으로 나타내는 積算溫度가 있으나, 넓은 지역의 生育分布를 조사할 때는 결과적으로 局地的인 작은 變化가 제거되므로, 검토하기가 용이하고 실제에 가까운 결과를 나타낼 수 있는 溫量指數를 代用하며, 그 補助로서 추위指數를 흔히 쓰고 있다. 本 硏究에서는 우리나라의 南海沿岸에 속하고 있는 20個地點의 氣象觀測所에 의하여 1970年 1月부터 1983年 12月까지(數個 地點은 비교적 短期 觀測値를 資料에 의거 利用했음)에 걸쳐 觀測된 月平均氣溫의 平均値를 각각 算定하고(그림-1), 各地點의 季節別 平均氣溫 分布(그림-2,3,4 및 5)를 考奈하였으며, 이를 基礎資料로 하여, 式(1)과 (2)에 의하여 各 地點에 대한 溫量指數와 추위指數를 그림 (6)과 같이 구하였다. 溫量指數의 分布는 대체로 약 100∼118℃에 이르며, 全地點에 대한 平均分布는 약 108.5℃ 가량을 나타냈으며, 특히 이러한 分布는 南海沿岸地方이 氣候學的 特性面에서 暖帶性 氣候帶에 속하며, 照葉樹林帶의 植物帶를 이루고 있음을 類推할 수 있게 하였고, 한편 溫量指數의 補助指數인 추위指數의 分布는 대체로 약 -4∼14℃에 이르며, 全地點의 平均은 약 -9.1℃ 가량을 보였고, 最寒月은 1月中으로서 平均氣溫이 약 0.6℃를 나타내었다. 이러한 結果는 旣히 硏究報告된 바와 대체적으로 잘 一致됨을 알 수 있다. 특히 溫量指數는 濕潤氣候下에 있는 森林帶의 分布 硏究에 有用한 指數로 널리 알려지고 있으며, 農作物과 氣溫과의 관계에서 따뜻한 정도를 나타내는 指數이므로 農作物의 生産力을 나타내는 指數가 될 수 있어서 農作物을 비롯한 植物의 生育分布 硏究에 기여 됨이 있을 것으로 思料한다. 끝으로 本 硏究遂行 過程에서 많은 基礎資料의 統計的 處理에 手苦해 준 慶尙大學校 農科大學의 金君渡군께 感謝를 드리는 바이다. This study was carried out to estimate the index of warmth and coldness of twenty-six sites in south coast of Korea. The most of all meteorological materials of monthly mean air-temperature used had been measured during fourteen years(Jan. 1970∼Dec. 1983) by the Meteorological Offices and Auxiliary Stations as shown Table 1. From the results of this study, the range of the warmth and coldness indices were distributed about 100℃∼118℃ and -4℃∼-14℃ respectively as shown Fig. 6. And then, the mean value of warmth and coldness indices in south coast of Korea were about 108.5℃ and -9.1℃ respectively.

Isolation of Antibodies Against the Spike Protein of SARS-CoV from Pig Serum for Competitive Immunoassay

정재용,봉지홍,김태헌,성정수,이창규,강민정,김현옥,신현진,변재철 한국바이오칩학회 2021 BioChip Journal Vol.15 No.4

Several endemic corona viruses (eCoVs) have been reported to be the most common etiologic agents for the seasonal common cold and also cause pneumonia. These eCoVs share extensive sequence homology with SARS-CoV-2, and immune responses to eCoVs can cross-react with SARS-CoV-2 antigens. Based on such cross-reactivity of antigens among eCoVs, the IgG antibodies against the spike protein (SP) of severe acute respiratory syndrome coronavirus (SARS-CoV) were isolated from pig serum using magnetic beads immobilized with SARS-CoV SP and a protein-A column. The selectivity of the isolated antibodies was tested using different types of antigens, such as SARS-CoV-2 nucleoprotein (NP), influenza A virus (Beijing type), influenza B virus (Tokio and Florida types), human hepatitis B virus surface antigen (HBsAg), and bovine serum albumin (BSA). From the selectivity test, the anti-SP antibodies isolated from pig serum had sufficient selectivity to other kinds of viral antigens, and the apparent binding constant of the isolated antibodies was approximately 1.5 × 10 –8  M from the surface plasmon resonance (SPR) measurements. Finally, the isolated anti-SP antibodies were applied to the immunoassay of SP using competitive immunoassay configuration. The feasibility of the detection as well as the quantitative analysis of the SARS-CoV viral culture fluid was determined using four viral culture samples, namely, SARS-CoV, SARS-CoV-2, MERS-CoV, and CoV-229E.

Competitive Immunoassay of SARS‑CoV‑2 Using Pig Sera‑Derived Anti‑SARS‑CoV‑2 Antibodies

봉지홍,김태훈,정재용,이수정,성정수,이창규,강민정,김현옥,변재철 한국바이오칩학회 2021 BioChip Journal Vol.15 No.1

Anti-severe acute respiratory syndrome coronavirus 2 (anti-SARS-CoV-2) nucleoprotein (NP) antibodies were isolated from pig sera using human SARS-CoV-2 NP-immobilized magnetic beads. The binding properties of the isolated antibodies against SARS-CoV-2 NP were tested via flow cytometry using SARS-CoV-2 NP-immobilized magnetic beads. A competitive immunoassay was developed for detecting SARS-CoV-2 NP as well as SARS-CoV-2 in the culture fluid using magnetic beads with immobilized anti-SARS-CoV-2 NP antibodies. Selectivity tests were carried out during the competitive immunoassay for SARS-CoV, MERS-CoV, and CoV strain 229E in the culture fluid.

An On-chip Chemiluminescent Immunoassay for Bacterial Detection using in Situ-synthesized Cadmium Sulfide Nanowires with Passivation Layers

김홍래,봉지홍,정재용,성정수,강민정,박재관,변재철 한국바이오칩학회 2020 BioChip Journal Vol.14 No.3

The passivation layers of an in situ-synthesized cadmium sulfide (CdS) nanowire (NW) photosensor were prepared for two reasons: (1) to improve the physical stability of the NW on an interdigitated electrode and (2) to enhance the immobilization efficiency of proteins for the on-chip immunoassay. The passivation layer was estimated to have suitable optical properties, and the photoresponse of photosensor was increased after the formation of passivation layer. The immobilization efficiency of a parylene-H film through covalent bonding was compared with the immobilization of Z-domains through physical adsorption. Finally, on-chip chemiluminescent immunoassay of bacteria was carried out by immobilizing antibodies against Escherichia coli through Z-domains for the orientation control of antibodies. The limit of detection was determined to be less than 104 E. coli/mL (n=3), and the sensitivity of bacterial detection was estimated to be 0.339 pA/E. coli/mL (n=3) with a linearity factor (R2) of 0.999. These results showed that the on-chip chemiluminescent immunoassay for bacterial detection could be performed using passivation layer coated CdS NW photosensor.

Pig Sera-derived Anti-SARS-CoV-2 Antibodies in Surface Plasmon Resonance Biosensors

봉지홍,김태훈,정재용,이수정,성정수,이창규,강민정,김현옥,변재철 한국바이오칩학회 2020 BioChip Journal Vol.14 No.4

Anti-coronavirus disease-2019 (COVID-19; anti-severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2)) antibodies against nucleoprotein (NP) were purified from pig sera. Following the separation of the antibody fraction using a protein-A column, the final yield of the purified antibodies against SARSCoV- 2 NPs was estimated to be 0.26 ± 0.05 % (absolute amount of 143.4 ± 25.2 ng, n=5) from 1 mL of pig sera. The binding activities of the isolated antibodies were confirmed using immunoassay and immunostaining. Based on the specific binding activity to NPs, a quantitative assay was performed using a surface plasmon resonance (SPR) biosensor. From the doseresponse curve, the binding constant (Kd) was calculated to be 185 pM and the limit of detection was estimated to be 1.02 pM. The SPR biosensor with the isolated antibodies against SARS-CoV-2 NPs was applied for the detection of SARS-CoV-2, MERSCoV, and CoV strain 229E in culture fluid.