원예적 환경에서 적용한 원예치료 사례연구- 발달장애 아동대상으로 -

윤경은,김희성 인간식물환경학회 2003 인간식물환경학회지 Vol.6 No.4

원형질체 분리, 배양 및 Nicotiana 종간 세포융합에 관한 연구

윤경은,김준철,최상수,손세호 한국연초학회 1979 한국연초학회지 Vol.1 No.2

For the preliminary study on tobacco cell fusion as one of new breeding techniques, the conditions that would be most effective in isolation, fusion, and culture of tobacco protoplasts were examined ; 1. The enzyme solution of 0.5% macerozyme and 2% cellulase( or meicellase) was the most economic and efficient in isolating protoplasts from tobacco leaves. 2. The proper incubation period of tobacco leaves in cell wall digesting solution was 4 hours. 3. As an osmotic stabilizer, sorbitol or mannitol solutions were employed. The concentration of 0.5~0.7 M of either hexitol gave satisfying results as the osmotic stabilizer. 4. The calcium concentration appeared to be an important factor in protoplast fusion. The adhesion of protoplasts was enhanced by enrichment of calcium ion in PEG solution. The highest frequency of protoplast fusion was obtained when tobacco protoplasts were incubated in PEG solution. containing 9mM CaCl2. 5. Cell divisions of the isolated protoplasts were continued and have generated colonies when they were grown on B-5 medium at 28$^{\circ}C$. 본 시험은 담배 신품종 육종기술확립을 위하여 효율적으로 原形質體(protoplast)를 얻을수 있는 방법과 protoplast 배양조건을 조사하였다. 1. protoplast를 효율적이며 경제적으로 얻을 수 있는 세포붕괴, 細胞模解離 酵素의 농도는 0.5% macerozyme + 2% cellulase (또는 meicellase)였다. 2. 효소처리시간은 품종간에 약간의 차이는 있었으나 4시간 이상이 필요하였으며 1인 작업량으로 보아 4시간이 가장 적합하였다. 3. 等張液을 만들기 위하여는 0.5∼0.7M의 mannitol이나 sorbitol을 이용하는 것이 좋았다. 4. 세포융합시에 Ca++ 이온의 농도는 중요하며 9mM CaCl2를 포함한 PEG용액(0.5g/ml)을 쓰는 것이 가장 효과적이었다. 5. 분리된 protoplast는 B-5 培地에서 계속분열하여 colony를 형성하였다.

Woman보다는 Human을 생각하는 서울여자대학교

윤경은,Yun, Gyeong-Eun 한국대학교육협의회 1999 大學敎育 Vol.100 No.-

바람직한 총장의 역할

윤경은,Yun, Gyeong-Eun 한국대학교육협의회 2002 大學敎育 Vol.119 No.-

전기영동에 의한 동양란(Cymbidium spp.)의 단백질 유형비교

윤경은,홍성숙,이남희 서울여자대학교 자연과학연구소 1991 자연과학연구논문집 Vol.2 No.-

본실험은 전기영동을 이용하여 단백질과 동위효소의 유형을 비교 분석함으로 종과 품종을 구분할 수 있는가의 가능성을 조사하고 전기영동에 적합한 난의 부위와 효소의 종류를 선정코자 실시하였다. 1. 한국춘란은 부위별로 꽃봉오리, 뿌리끝, 어린잎, 성엽을 6% polyacrylamide slab gel로 단백질을 비교한 결과 부위별로 유형을 달리하였으나 band의 분리가 명확하지 않았다. 2. 10% SDS polyacrylamide slab gel에서는 band의 분리가 명확하였고 영동재료로는 어느 때나 쉽게 채취가 가능한 잎이나 뿌리를 사용하는 것이 편리하나 뿌리는 phenol 함량은 적으나 단백질 함량이 극히 낮으므로 잎을 시료로 쓰는 것이 양호한 편이었다. 3. 잎을 시료로 쓰는 경우는 엽록소, phenol의 제거가 필요하나 배양중인 rhizome을 사용하면 이러한 난점이 없이 단백질 유형이 뚜렷이 나타나, 춘란(Cymbidium goeingii), 한란(C, kanran), 죽백란(C, lancifolium)을 구분할 수 있었다. 4. Rhyzome의 esterase, peroxidase, acid phosphatase, glucose phosphate isomerase의 동위효소를 비교한 결과 단백질 유형으로는 분류가 어려웠던 춘란(Cymbidium goeringii)과 춘란복륜(Cymbidium goeringii:variated leaves)도 구분할 수 있었다. Peroxidase는 band는 여러가지로 나타났으나 esterase와 같이 band pattern이 명확지 못하였고 acid phosphatase와 glucose phosphate isomerase는 한개씩의 band만이 나타나 분류에는 적합하지 않은 효소로 생각되어졌다. The buffer soluble proteins were extracted from flower, root, young leaves, old leaves, and cultured rhizome. Different patterns of general protein were observed among the tested organs. Among the samples rhizome were the most suitable for electrophoresis to differentiate cultivars. Band patterns of general protein, esterase, peroxidase showed typical band patterns among Cymbidium goeringii, Cymbidium kanran, and Cymbidium lancifolium which have similar appearance of vegetative leaves. The isozymes of esterase, peroxidase, acid phosphatase, and glucose phosphate isomerase in cultured rhizome were analized. The well-resolved zones of esterase activity were detected. The differentiation of Cymbidium goeringii and Cymbidium goeringii : variated leaves which was hard to differentiated by general protein patterns, could be done by esterase isozyme band pattern.

법률 번역 교육 과정에 대한 수요조사와 그 시사점

윤경은 한국통번역교육학회 2014 한국통번역교육학회 학술대회 자료집 Vol.2014 No.2

일반두 (一般豆)와 장간 (長稈) 대두에 있어서의 지베레린 및 ABA 함량의 차이

윤경은 한국콩연구회 1985 韓國콩硏究會誌 Vol.2 No.2

大豆 品種 Flambeau와 Shinsei를 交配하였을 때 兩親보다 월등히 稈長이 긴 系統을 얻었다. 이 계통의 F_6에서 얻은 長稈形系統을 Super Tall이라 命名하고 이와같이 특이하게 절간이 길어지는 原因을 硏明코자 本實驗을 實施하였다. 1. Super Tall은 外形上으로 일반 大豆에 지베레린 處理를 한 것과 같이 節間이 길어지고 葉柄도 길어지며 잎이 細葉形을 나타내므로 Super Tall의 節間길이 生長促進에 지베레린이 관여하는지를 調査하였다. 2. 파종후 15일된 大豆 幼苗에서지베레린을 추출하여 부분적으로 순화하고 박층크로마토그라피를 하여 지베레린을 分離하였다. 지베레린의 含量은 短稈 交配親인 Flambeau 보다 Super Tall에 多量 含有되어 있었다. 3. 지베레린 含量 自體보다 大豆가 지베레린에 대한 反應性에 差理가 있는가를 알기 위하여 低溫 또는 高溫에서 大豆를 키우며 지베레린을 處理하거나 지베레린 生成 抑制劑인 AMO- 1618을 處理한 後 大豆의 生育反應을 調査하였다. 大豆는 低溫(21°/15℃)에서 보다 高溫(27°/21℃)에서 草長이 길고 生體重이 높았다. 지베레린 處理 效果는 高溫에서보다 低溫에서 컸으나 AMO- 1618의 效果는 低溫보다 高溫에서 컸다. 이는 低溫에서 자란 大豆보다 高溫에서 자란 大豆가 지베레린 含量이 높았던 것으로 解析되었다. 같은 溫度에서도 지베레린의 節間生長促進效果는 長稈形의 Super Tall 보다 같은 系統의 短稈形인 Short Sister Selection 에서 그 效果가 뚜렷하였다. AMO-1618의 效果는 短稈形의 Short sister Selection보다 Super Tall 에서 컸다. 이로 미루어 볼때 Super Tall은 Short Sister Selection보다 內在한 지베레린 含量이 많은 것이지 주어진 지베레린 含量에 대하여 예민한 反應을 나타낸 結果로 節期이 월등히 자란 것은 아니라 解析된다. 4. Super Tall은 交配親인 Flambeau나 Shinsei보다 生長抑制物質 含有量이기었으며 그 抑制物質의 주된 성분은 ABA(Abscisic acid)로 確認되었다. 5. ABA를 추출 순화하여 比較한 結果 短稈種 Flambeau에 가장 많이 含有되어 있고 다음이 Shinsei, 그리고 長稈系統의 Super Tall에 그 含量이 가장 낮았다. 6. 以上의 結果로 볼때 Flambeau와 Shinsei의 交配에서 얻은 長稈形 系統의 유난스러운 節間成長은 Super Tall 植物體內에 生育促進劑인 지베레린 含量은 높고 抑制物質인 ABA含量은 낮은 結果 때문이라 思料된다. This research was conducted to find the possible controlling mechanism of the extraordinary stem elongation of soybean breeding line Super Tall which was a F_6 offspring derived from a cross of Flambeau and Shinsei. The phenotype of Super Tall closely resembled to soybean plants treated with exogenous gibberellic acid (GA). The involvement of GA in Super Tall stem growth was studied. GA was extracted from 15-day-old soybean plants, partly purified, and subjected to thin layer chromatography. Gibberellin activity was assayed with dwarf rice seedlings. The assay demonstrated that Super Tall contained higher level of gibberellin and lower level of inhibitors than its parent cultivars did. To clarify the cause of drastic stem elongation of Super Tall was the result either of high efficiency of GA production and turnover, or of high sensitivity of soybean to GA, plant response to the application of exogenous GA₃ and GA biosynthesis inhibitor, AMO-1618 were measured. Elevated temperature enhanced the shoot growth and increased fresh weight of soybeans. Temperature affected responsiveness of soybean to exogenous GA₃ treatment. Application of exogenous GA₃ was less effective at high temperature than at low temperature. The inhibitory effect of AMO-1618 on stem elongation was more apparent at high temperature (27˚/21℃) than at low temperature (21˚/15℃). This suggest that the endogenous level of GA was higher at high temperature than at low temperature. The stimulatory effect of GA₃ on stem growth of soybeans was much greater in Short Sister Selection than in Super Tall, and the inhibitory effect of AMO-1618 on the soybean growth was more distinctive in Super Tall than in Short Sister Selection. This may be interpreted as the result of the higher level of endogenous gibberellin in Super Tall than in Short Sister Selection, but not the result of the higher sensitivity of Super Tall to gibberellin. Abscisic acid (ABA) was extracted, partially purified, and separated by thin layer chromatography. The ABA activity was assayed by wheat coleoptile elongation in the dark. The level of ABA was high in short parent cultivar Flambeau, low in Super Tall, and medium in Shinsei. In conclusion, it is proposed that the enhanced stem elongation of Super Tall was the result of the combination of high level of endogenous GA and low level of ABA.

원예작물 별poly-plenol 함량과 항산화력

윤경은,김안나 인간식물환경학회 2007 인간식물환경학회지 Vol.10 No.3

중년기 기혼여성의 삶의 질 향상에 있어 원예의 역할

윤경은 서울여자대학교 여성연구소 2003 여성연구논총 Vol.18 No.-

The role of horticulture in enhancement of quality of life of married women in middle age can be explored in four areas; well-beings of physical, mind-body, emotional and social. Each of these areas is obtained from a unique interaction between human and horticulture. Horticulture plays a role in maintaining our body, but also helps us to enrich our mind and soul as we observe, nurture and share the life around us. Kleingarten(or week-end farm), horticultural therapy, and healing gardens were reviewed as programs which may have many benefits and meaning in quality of life of married women in middle age.

법률번역 교육 수요자 요구조사와 시사점

윤경은 한국통번역교육학회 2014 통번역교육연구 Vol.12 No.3

Thanks to the important roles legal translation plays in ever-increasing international exchanges, there has been much research effort both home and abroad. The ultimate goal of such research is to improve the quality of legal translation, which can be achieved through a practical approach, ‘training’. The first step to design a training program is to understand the needs of its consumers. Against this backdrop, a survey of learners who enrolled in the non-degree course on 'Professional Legal Translation' offered by the Graduate School of Translation and Interpretation at Ewha Womens University was conducted. The survey contained 23 closed-ended questions and one open-ended question canvassing 25 trainees' personal backgrounds including their education, workplaces and legal translation experiences, difficulties in legal translation and opinions for improvement. The results indicate that trainees can be divided into two groups, law majors and translation majors, who are apparently different in their needs of legal knowledge and language competence. For more effective training, this study suggests a program with different tracks and levels which learners can choose according to their needs.