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밤의 Polyphenol물질과 Polyphenol Oxidase의 생화학적 특성
孫泰華,文廣德,尹基潤 慶北大學校農業科學技術硏究所 1991 慶北大農學誌 Vol.9 No.-
밤과실의 가공 및 저장중 갈변기구를 규명하는 일환으로 밤에 함유된 polyphenol 화합물 및 PPO를 분리, 동정하고 PPO의 생화학적 특성에 대해 연구한 결과는 다음과 같다. 밤의 total phenol 함량은 6.5㎍/g이었고 ferulic acid, caffeic acid, synapic acid, pcoumaric acid, gallic acid, salicylic acid 순으로 함량이 높았다. PPO를 분리, 정제하여 전기영동한 결과 단일의 단백질 및 효소활성밴드를 확인하였으며 정제된 효소의 비활성도는 260.9이고 조효소액보다 11.7배 정제되었다. PPO의 최적 pH와 온도는 각각 5.9, 45℃였으며 효소활성은 80℃에서 15분 처리시 거의 실활되었다. 무기염의 영향을 본 결과 ??, ??, ??들은 효소활성을 증가시켰으나 ??, ??, ??는 활성을 저해시켰다. 저해제로는 L-ascorbic acid, thiourea, sodium chloride, L-cysteine의 저해작용이 강했다. 밤의 PPO는 o-diphenol에 대한 강한 활성을 나타냈으며 특히 catechol에 대해 가장 강했으며 monophenol에는 활성을 나타내지 않았다. PPO의 Km값은 catechol에 대해 5mM이었다. This study was conducted to understand browning characteristics of Chinese Chestnut during processing and storage. For this, the isolation and identification of polyphenolic compounds and the biochemical characteristics of polyphenol oxidase(PPO) were investigated. The content of total phenol was 6.5㎍/g and it was consisted of ferulic acid, caffeic acid, synapic acid, p-coumaric acid, gallic acid and salicylic acid in order. PPO was purified 11.7 fold through ammonium sulfate fractionation, DEAE-cellulose column chromatography and Sephadex G-200 column chromatography. Purified enzyme showed single protein and activity band by polyacrylamide gel electrophoresis. The optimum pH and temperature of PPO were 5.9 and 45℃, respectively. The activity of PPO was lost 93% by exposing at 80℃ for 15minutes. ??, ??, ?? increased the activity of PPO, but ??, ??, ?? inhibited PPO at 10mM concentration. L-ascorbic acid, thiourea, sodium chloride and L-cystein were effective inhibitors of PPO. The activity of PPO was higher for o-diphenols than other polyphenols. The Km value of PPO for catechol was 5mM.
Sodium Levothyroxine의 造血促進 效果에 關한 實驗的硏究
尹基潤,黃基錫 慶北大學校 醫科大學 1970 慶北醫大誌 Vol.11 No.1
The hemopoietic effect of sodium levothyroxine in the albino rats was studied with the following results. 1) In rats administered sodium levothyroxine orally at 4 ㎍/㎏ of body weight daily for four week's, a significant rise in reticulocyte was noted whereas no noticeable changes were found in hemoglobin, neutrophil and platelet. Also no appreciable differences were observed in nucleated cell and megakaryocyte counts in marrow, RBC ^59Fe uptake and plasma ^59Fe disappearance half time as compared with those of the control group, 2) In the rats treated with an oral administration of sodium levothyroxine at 40㎍/㎏ of body weight daily for four weeks, significant increases in hemoglobin and reticulocyte were observed whereas both neutrophil and platelet revealed no significant changes, the nucleated cell count and megakaryocyte counts in the marrow as well as the plasma ^59Fe disappearance half time were not significantly altered but only the RBC ^59Fe uptake was increased as compared with those of the control group. 3) In rats given sodium levothyroxine at 400ug/㎏ of body weight daily for four weeks, both hemoglobin and reticulocyte were increased remarkably compared with those of the 40ug-administered group although neutrophil and platelet were not changed. As compared with those of the control group, both increased nucleated cell count, in the marrow RBC ^59Fe uptake, and shortened plasma ^59Fe disappearance half time were noted but only the megakaryocyte count disclosed no variation. 4) Rats given sodium levothyroxine orally at 40㎍/㎏ of body weight daily for four weeks after a total 3.0ml. of blood had been drawn, exhibited earlier recovery of hemoglobin as well as greater increase in reticulocyte as compared with the control group in which only blood had been drawn.