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DEI 달성을 위한 초급 한국어 디지털 교과서 구축 방안
송재영 국제한국어교육학회 2022 한국어 교육 Vol.33 No.4
The purpose of this study is to improve learners’ Korean language skills through diversity, equity, and inclusion (DEI). This study examines how DEI could be achieved by developing a novel digital textbook for the beginning level of Korean classes at Wellesley College. First, inclusiveness was accomplished by setting the Wellesley students as the main characters and using the main living spaces of Wellesley students as the background. When establishing the main characters, diversity was expanded by considering race, gender identity, disability, and various learning goals. To promote equity, the textbook was built as a digital form and provided free of charge, so that learners could easily use various learning tools regardless of their geographic location or socioeconomic status. In the process of transitioning to in-person classes, I discuss ways to utilize digital textbooks in offline classes and seek directions for future development through feedback from learners.
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Baculovirus 를 이용한 Aujeszky's Disease Virus gIII 단백질 발현
송재영,이중복,현방훈,박종현,김병한,권창희,전무형,안수환 대한바이러스학회 1992 Journal of Bacteriology and Virology Vol.22 No.2
The gIII gene located in U region of Yangsan strain, a field isolate of Aujeszkys disease virus (ADV) in Korea, was cloned into pTZ18R and sequenced. The glll gene consisting of 1,437 nucleotides showed 98 % sequence homology with that of Becker strain, a reference strain of ADV. The gene encoding gK of Yangsan strain was placed under the control of Autographa californica nucleopolyhedrosis virus (AcNPV) polyhedrin promoter, and expressed by the derived recombinant baculovirus using Spodoptera frugiperda 9 (Sf9) cells. The expressed g Ill was a protein with molecular weight of 72kd determined by immunoprecipitation and Western blotting assay using anti-ADV polyclonal antibodies and anti-g IE monoclonal antibody. The partially purified g lll protein was utilized as antigen in the radial immunodiffusion enzyme assay (RIDEA) to detect the specific antibody against ADV in pig sera. The results indicated that the sensitivity of RIDEA with the recombinant gIll protein antigen (98% ) was as high as that with the conventional glycoprotein antigen extracted from the ADV infected cells. In addition, the false positive and false negative reactions in gIE RIDEA were significantly reduced than the conventional glycoprotein RIDEA as judged from the results of standard serum neutralization test.
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AZCA 저항성 돌연변이 세포주로부터 선발 육성만 내염성 벼 돌연변이 계통의 특성 검정
송재영,김동섭,이긍주,이인석,강권규,윤성중,강시용,Song, Jae-Young,Kim, Dong-Sub,Lee, Geung-Joo,Lee, In-Sok,Kang, Kwon-Kyoo,Yun, Song-Joong,Kang, Si-Yong 한국식물생명공학회 2007 식물생명공학회지 Vol.34 No.1
본 연구는 벼 배 배양 캘러스에 방사선 조사와 AZCA 처리를 통해 AZCA저항성 세포주를 선발 육성하고 proline 함량이 증가된 선발 계통을 중심으로 염분 스트레스에 저항성을 갖는 벼 계통을 육성하고 그 기작을 밝히고자 하였다. 먼저, 1) AZCA 저항성 후대로부터 NaCl 저항성 식물체를 선발하고, 2) 선발된 저항성 계통의 생리적 생화학적 특성을 분석하였으며, 3) 분자적 특성을 RT-PCR을 통해 조사하고 유전적 변이를 탐색하였다. AZCA저항성 $M_{3}$ 3,000 계통으로부터 얻어진 약 20,000 종자에 염분 적정 선발 농도로 밝혀진 1.5%의 염분을 처리하여 내염성 (ST) 벼 116 개체를 선발하고, $M_{4}$ 후대 세대를 양성하였다. $ST\;M_{4}$ 세대에서 2차 내염성 계통 선발을 위해서 $M_{4}$ 세대계통을 1.2% NACl 에서 대조구와 생육 조사한 결과, 대조구 식물은 생육이 약하고 성장이 지연되는 것을 볼 수 있었다. 내염성 계통(ST-13, ST-16)으로부터 유도된 캘러스에 NaCl 처리한 결과, 대조구, ST-13, ST-16의 생존율은 9%, 16%, 20%로 나타났다. 또한, 필수 아미노산 함량을 잎, 종자 및 캘러스로 나누어 분석한 결과 ST-13와 ST-16는 대조구와 비교하여, 1) 잎에서는 약 1.24, 1.3배, 2) 종자에서는 1.49, 2.43배, 3) 캘러스에서는 1.32, 1.60배 증가하는 것이 확인되었다. 내염성 계통과 대조구에서 이온함량을 비교한 결과 잎과 뿌리에서 $K^{+},\;Na^{+}$ 및 $Na^{+}/K^{+}$ 비율을 보면 대조구보다 $Na^{+}/K^{+}$ 비율이 낮아진 것이 확인되었다. 내염성과 연관된 유전자 P5CS, NHXI를 이용하여 RT-PCR 실험을 수행한 결과, 돌연변이 계통에서 이들 유전자의 발현이 증가됨을 확인할 수 있었다. 본 연구에서 선발된 계통은 내염성 육종 및 기초 연구를 위한 재료로 이용될 수 있을 것으로 사료된다. To develop rice (Oryza sativa L.) cultivars to be planted on salt-affected sites, cell lines with enhanced proline content and resistance to growth inhibition by Azetidine-2-carboxylic acid (AZCA), a proline analogue, were screened out among calli irradiated with gamma ray of 50, 70, 90, and 120 Gy. The calli had been derived from embryo culture of the cultivar Donganbyeo. Selected AZCA resistant lines that had high proline accumulation were used as sources for selection of NaCl resistant lines. To determine an optimum concentration for selection of NaCl resistant lines, Donganbyeo seeds were initially cultured on the media containing various NaCl concentrations (0 to 2.5%) for 40 days, and 1.5% NaCl concentration was determined as the optimum concentration. One hundred sixteen salt-tolerant (ST) lines were selected from bulked 20,000 seeds of the AZCA resistant $M_{3}$ seeds in the medium containing 1.5% NaCl. The putative 33 lines ($M_{4}$ generation) considered with salt-tolerance were further analyzed for salt tolerance, amino acid and ion contents, and expression patterns of the salt tolerance-related genes. Out of the 33 lines, 7 lines were confirmed to have superior salt tolerance. Based on growth comparison of the entries, the selected mutant lines exhibited greater shoot length with average 1.5 times, root length with 1.3 times, root numbers with 1.1 times, and fresh weight with 1.5 times than control. Proline contents were increased maximum 20%, 100% and 20% in the leaf, seed and callus, respectively, of the selected lines. Compared to control, amino acid contents of the mutants were 24 to 29%, 49 to 143%, 32 to 60% higher in the leaf, seed and callus, respectively. The ratio of $Na^{+}/K^{+}$ for most of the ST-lines were lower than that of control, ranging from 1.0 to 3.8 for the leaf and 11.5 to 28.5 for the root, while the control had 3.5 and 32.9 in the leaf and root, respectively. The transcription patterns for the P5CS and NHXI genes observed by RT-PCR analysis indicated that these genes were actively expressed under salt stress. The selected mutants will be useful for the development of rice cultivar resistant to salt stress.
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송재영,배진주,한지원,고호철,이호선,남성희,이정로,윤병현,김금선,원경호,신일섭 한국자원식물학회 2023 한국자원식물학회지 Vol.36 No.6
Abstract - In this study, cryopreservation by droplet-vitrification was applied to pear (Pyrus spp.) germplasm. We focused on the development and assessment of various strategies for the selection of suitable tissue, osmoprotection, and dehydration. We also evaluated post-thaw recovery of cryopreserved explants by droplet-vitrification. Preferentially, we tested the effects of preculture and loading treatments to determine which tissues were more suitable, either the apical shoot tips or the axillary buds. Apical shoot tips showed the better regrowth rate than in vitro axillary buds. The most effective techniques for cryopreservation were as follows. Shoots from in vitro seedlings which had been cultured for about 5-6 weeks were coldhardened at 4℃ for one week, excised shoot tips were precultured on liquid MS medium including 0.3 M sucrose for 31 hours and 0.7 M sucrose for 17 hours, osmoprotected in loading solution (LS) for 40 min, and then cryoprotected in dehydration solution (PVS3) for 90 min. In addition, we found that regrowth rates of explants on regrowth medium after exposure to liquid nitrogen (LN) were higher than those on MS medium. Results indicated that the highest regrowth percentage was 95.6% for ‘Bartlett’ cultivar and 68.9% for ‘BaeYun No.3’ cultivar. Consequently, apical shoot tips of two pear cultivars, ‘Bartlett’ (P. communis) and ‘BaeYun No.3’ (P. pyrifolia), were successfully cryopreserved by droplet-vitrification. Results of this study show that the enhanced droplet-vitrification method described in the present study could be used as an effective means for long-term storage of pear genetic resources.
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