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감자 괴경으로부터 유도된 Shoot의 발생 단계별 peroxidase isozyme 양상
송규상,김영희,정현숙 조선대학교 부설 유전생물공학연구소 1992 유전생물공학연구지 Vol.2 No.-
The callus was induced from potato tuber on modified MS medium containing 10 mg/1 α-naphthaleneacetic acid and 2,4-dichlorophenoxyacetic acid 2 mg/1 and somatic embryo was formed in suspension-cultured callus cells. Also, the shoot from microtuber discs was cultured on MS medium And then, we investigated peroxidase activity and patten in callus, somatic embryo induced from potato tuber and shoot induced from potato microtuber in order to study of the physiological and biochemical changes on the developmental stage. The peroxidase activity was higher about 80% in somatic embryogenic cells than callus after 7 weeks culture. And peroxidase isozyme pattern observed 4 cathodic bands and 4 anodic bands by isoelectric focusing and 2 new bands were revealed in somatic embryogenic cells. Addition, in callus 2 cathodic bands and 1 anodic band were revealed, and new pI 3.9 band was observed at 7 weeks culture. The peroxidase activity in shoot induced from microtuber was decreased about 25% cultured shoot for 1 week but increased gradually about 60% after 4 weeks. The pattern of peroxidase isozyme of 4 weeks cultured shoot was observed 2 new bands by isoelectric focusing.
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송규상 충남대학교 의과대학 지역사회의학연구소 1996 충남의대잡지 Vol.23 No.2
To study angiogenesis and apoptotic indices in the brain tumors, 33 cases of brain tumor(13 cases of astrocytoma, 10 cases of glioblastoma multiforme and 10 cases of meningioma) were seldcted and stained with anti-factor Ⅷ monoclonal antibody and in situ apoptosis detection kit(Apoptag). Among brain tumors glioblastoma multiforme showed the highest angiogenic activity(25.4%). The average percentages of endothelial cells in grade I, Ⅱ, Ⅲ astrocytomas were 3.2, 5.6, 8.4%, respectively and 18.4% in meningioma. Apoptotic indices were highest in glioblastoma multiforme(2.7), followed by grade Ⅲ astrocytoma and meningioma. The results suggest that the glial tumors(astrocytoma and glioblastoma multiforme) seem to show increasing tendency of angiogenesis and apoptotic index ac cording to the degree of malignancy.
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송규상 충남대학교 의과대학 지역사회의학연구소 1989 충남의대잡지 Vol.16 No.2
An immunohistochemical study was performed on the normal salivary gland, pleomorphic adenomas, mucoepidermoid carcinomas, adenoid cystic carcinomas, acinic cell tumor, and Warthin’s tumor. PAP kits for S-100 protein, vimentin, and epithelial membrane antigen were used and the results were as follows. 1. Myoepithelial cells of the normal salivary gland were positive for S-100 protein and vimentin, negative for EMA. Duct cells were stained positively for S-100 protein and EMA. Acinic cells were negative for all stains. 2. Neoplastic myoepithelial cells were strong positive for S-100 protein and vimentin. Areas of ductal differentiation were positive for EMA and occasionally positive for S-100 protein and vimentin. 3. Mucin secreting cells and squamous cells of mucoepidermoid carcinoma showed posive reaction for EMA. Intermediate cells revealed weak positivity for S-10 protein and EMA and were positive for vimentin. 4. Adenoid cystic carcinoma showed occasional positivity for S-100 protein and vimentin. Ductal areas were positive for EMA. 5. Warthin’s tumor showed positive reaction in epithelial cells for EMA. Acinic cell tumor were negative for all antibodies. These results suggested that the coexpression of S-100 protein and vimentin is useful in identify-ing the neoplastic myoepithelial cells and the myoepithelial cells play an important role in the histogenesis of pleomorphic adenomas, mucoepidermoid carcinomas, and adenoid cystic carcinomas.
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유방병변에서의 Nucleolar Organizer Regions에 대한 연구
송규상 충남대학교 의과대학 지역사회의학연구소 1992 충남의대잡지 Vol.19 No.2
To study the proliferative activity of cells in benign and malignant breast lesions, a one step silver staining technique to identify argyrophilic nucleolar organizer regions had been applied in 11 cases of fibrocystic change, 11 cases of fibroadenoma, 15 cases of invasive ductal carcinoma without metastasis, 9 cases of invasive ductal carcinoma with metastasis, and 3 cases of medullary carcinoma, respectively. The results were as follows: 1. AgNORs in both fibrocystic disease and fibroadenoma were relatively small and uniform in size and shape. Epothelial cells in fibrocystic change had 1.49 AgNORs per cell in average Stromal cells and epithelial cells in fibroadenoma contained 1.58 AgNORs and 2.02 AgNORs per cell in average, respectively. 2. Invasive ductal carcinomas and medullary carcinomas showed relatively irregular AgNORs. The mean numbers of AgNORs in invasive carcinoma with or without metastasis were 3.23 and 3.41 per cell. Medullary carcinomas contained 3.18 AgNORs per cell. There were no statitical differences among malignant tumors. 3. The numbers of AgNORs were statistically different between benign and malignant lesions. These data suggested that the mean AgNORs counts was useful in differentiation between benign and malignant breast lesions. However, it seemed that they did not correlate with malignant types and status of metastasis.
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송규상,권수진,김헌수 충남대학교 의학연구소 2003 충남의대잡지 Vol.30 No.2
To investigate the relationship between mucin phenotypes and prognostic factors in gastric adenocarcinoma, immunohistochemical stainings against antibodies(Muc 5AC, Muc-6, Muc-2, CD10, HMG 45M1, and p53 were performed in 104 cases of adenocarcinomas of the stomach. The most frequently expressed mucin phenotype is mixed mucin phenotype, followed by gastric mucin phenotype and intestinal mucin phenotype. In terms of invasiveness and lymph node metastasis, gastric adenocarcinoma with mixed mucin phenotype seems to be more aggressive than those with gastric and intestinal mucin phenotypes. There is no significant difference in expression of p53 protein among three mucin phenotypes. These results suggest that mixed mucin phenotype is the most frequently expressed mucin phenotype in gastric adenocarcinomas and seems to be associated with aggressiveness.
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백서의 복강내에 투여한 Mitomycin-C-Polyglycolic acid 제형이 장기에 미치는 영향에 관한 연구
송규상,강대영,최정목,노승무,정경수,오정연,김진향,양준목,최선웅,이진호,조준식,민병무,김용백,김창식,박근성,김승영,김학용,인현빈 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1
To evaluate the in-vivo effects of mitomycin-C-polyglycolic acid composite(MMC-PGA) the disk: shaped composite were implanted into the peritoneal cavity of the Sprague-Dawley rats. The pathologic changes were examined at various time points up to 12 weeks. Initially the soft tissue around the inserted disks showed capillary congestion at 3 days. Inflammatory infiltrates with foreign body giant cells appeared from the 2nd week and reached peak response at 6-8 weeks. These reaction diminished prominently at 12 weeks. No specific pathologic change was found in the liver, the kidneys, and the heart. The above results suggest that the MMC-PGA composite can serve as a new device for intraperitoneal chemotherapy of various types of cancers.
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