Effect of 3 - Aminobezamide on DNA Fragmentation Induced by Mitomycin C and Cytotoxic Lymphocyte

손정희,김영상,민들레,황대연 한국유전학회 1995 Genes & Genomics Vol.17 No.4

The poly (ADP-ribose) polymerase (pADPRp) was found to be involved in DNA repair, DNA replication and recombination etc. There were several reports suggesting the involvement of pADPRp in apoptosis process, but only in limited conditions. To study the function of pADPRp in the apoptosis induced by alkylating agent or by cytotoxic lymphocyte, various cells were treated with a pADPRp in the apoptosis induced by alkylating agent or by cytotoxic lymphocyte, various cells were treated with a pADPRp inhibitor, 3-aminobenzamide (3-AB), as well as mitomycin C (MMC) or cytotoxic lymphocyte. P815 mouse mastocytoma, A20 B cell lymphoma and HL-60 human promyelocytic cells treated with MMC or cytotoxic lymphocyte showed DNA ladder of multiple nucleosomal sizes. DNA fragmentation induced by MMC was gradually decreased by increasing dose of 3-AB in HL-60 cells, but not in other cells. This result suggest that apoptosis signaling pathway in HL-60 cell, which is deficient in p53 expression, involves pADPRp, but not in others. The DNA fragmentation induced by cytotoxic lymphocyte was not affected by 3-AB in all the three cell types, suggesting that the apoptosis signaling pathway by MMC is different from that by cytotoxic lymphocyte.

지속가능발전교육을 위한 초등학생용 애니메이션 기반 생물자원 학습프로그램의 학습효과

손정희,길지현,소금현,여성희 韓國生物敎育學會 2013 생물교육 Vol.41 No.1

In this research, the biological resources animation learning program was developed for elementary school students in education for sustainable development (ESD). To examine learning effects of it, elementary students' conceptual understanding, attitude, and will to practice about biological resources related to sustainable development. Subjects were 108 elementary school elementary school, Incheon metropolitan city. Treatment group using biological resources animation learning program had higher understanding level of concepts compared to students of control group (p<0.5). Students who were applied to sustainable development education using animation showed more improved attitude about sustainable development of biotic resources than those who were applied to conventional course group (p<0.1). Students applied to sustainable development of biotic resources using animation showed improved will about sustainable development (p<0.1). They made their own values about sustainable development of biological resources by thinking together with the characters of animation and making decisions for characters. Especially, they showed much improved will from environmental prospective, and we assume that the effect of program is added to the pre-formed environmental value about biological resources and this works enormously.

소설의 TV드라마化에 따른 왜곡 논란 연구 - 소설 「철쭉제」와 TV드라마 『철쭉제』를 중심으로

손정희 한국문예창작학회 2006 한국문예창작 Vol.5 No.2

일부 보건소 모자보건실 연계 비외과적 치주처치 중심의 치위생관리 프로그램 운영 사례연구

손정희,김상희,배수명 한국콘텐츠학회 2019 한국콘텐츠학회논문지 Vol.19 No.2

This research was conducted by targeting pregnant women in the trial operation of dental hygiene care program with the focus on non-surgical periodontal treatments at the Maternal and Child Health center in the public health center. This study was conducted to provide basic data to dental hygienists who operate oral health promotion programs by targeting pregnant women of the local community through the evaluation on structure, the assessment process and results from the pilot program at the public health center. The targets are nine pregnant women who have been involved in the Dental hygiene care program three times. An evaluation on the structure, process and results according to the Indicators of each program were conducted. The results of dental hygiene care program found that the retention rate decreased. The retention of the probing pocket depth larger than 4mm was 40.4% to 14.0%(p<0.05). The retention rate of the gingival bleeding teeth was 4.6% to 2.7% (p=0.180). PHP Index improved. 1.7 points in the 1st to 1.4 points in the 3rd in the case of the second pregnant woman and 2.4 points in the 1st to 1.3 points (p<0.05) in the 3rd in the case of the third pregnant woman. The applicable and expandable possibility of the dental hygiene care program with the focus on non-surgical periodontal treatments at the Community Health Center is significant in the result of this study. In the future, long-term operation and ongoing monitoring of this program is needed. 본 연구는 제천시보건소 모자보건실을 이용하는 임산부를 대상으로 비외과적 치주처치 중심의 치위생관리 프로그램을 시범운영하고, 시범운영의 구조평가, 과정평가, 결과평가를 통하여 보건소에서 치과위생사가 지역사회 임산부를 대상으로 구강건강증진 프로그램을 운영하는데 기초자료로 제공하기 위해 수행되었다. 총 3회의 치위생관리 프로그램에 모두 참여한 9명의 임산부를 대상으로 하였다. 치위생관리 프로그램 결과 4mm 이상 치주낭 보유율은 40.4%에서 14.0%로(p<0.05), 치은출혈 치아 보유율은 4.6%에서 2.7%로(p=0.180), 치석형성 치아 보유율은 23.7%에서 0%로(p<0.05) 감소하였다. PHP Index(Patient Hygiene Performance index)는 2기 임산부의 경우 1차 1.7점에서 3차 1.4점으로 개선되었고, 3기 임산부의 경우 1차 2.4점에서 1.3점으로 개선되었다(p<0.05). 본 연구 결과 비외과적 치주처치 중심의 치위생관리 프로그램을 보건소 구강보건실에서 적용 및 확대할 수 있는 가능성이 높다는 것에 의의가 있으며, 향후 본 프로그램의 장기적인 운영과 지속적인 모니터링이 필요할 것으로 사료된다.

샬롯 퍼킨스 길먼: 사회를 치료하는 여성 작가/의사

손정희,한우리 한국외국어대학교 영미연구소 2014 영미연구 Vol.32 No.-

This paper aims to argue that an act of writing, for Charlotte PerkinsGilman, becomes a medium of political intervention into social reform aswell as a kind of therapeutic process not only for healing herself but alsofor affecting the fate of other women of her time. It is well known that Dr. Mitchell who treated Gilman with ‘rest cure’ supposedly changed histreatment after reading “The Yellow-Wallpaper.” This anecdote shows howGilman's writing served as a kind of medical treatment of a woman doctor,which influenced other fellow doctors. By analyzing her short stories suchas “The Yellow Wall-paper,” “Dr. Clair’s Place,” “Old Water,” “Making aChange,” “Beewise,” and “If I Were a Man,” and a poem “To the YoungWife,” this paper examines Gilman’s attempt to rectify the typical imagesof feminine women and to provide alternative images of healthy women,and by extension, a healthy society. Proposing a new paradigm for health,Gilman as woman writer/doctor becomes an active social reformer whoenvisions a feminist utopia.

치위생(학)과 학생을 위한 치위생윤리 교과목 콘텐츠 개발에 대한 연구

손정희,신선정 한국치위생과학회 2022 대한치위생과학회지 Vol.5 No.2

Background: This study aimed to create and present content that can be used in the dental hygiene ethics process to help dental hygiene students develop desirable work ethics and ethical values. Methods: In order to operate the dental hygiene ethics course in all academic systems, one three-year dental hygiene professor and one four-year dental hygiene professor participated in setting core competencies and learning goals for the dental hygiene ethics course. The class consisted of two credits, two hours of theoretical classes, and class activity sheets developed according to the learning contents and learning topics for each week that can be operated for 15 weeks. Results: The contents of the dental hygiene ethics subject were developed to be conducted as theoretical education and case-oriented discussion classes. The 15-week class consisted of a theory lecture on dental hygiene work ethics (eight weeks), discussions and presentations for ethical decisions based on actual cases related to dental hygiene ethics (four weeks), and the design and presentation of individual professional mission statements and codes of conduct (three weeks). The class data for each week consisted of four stages: “Learning goal-thinking,” “openthinking,” “learning content-thinking,” and “according to learning goal.” Conclusions: In order to establish desirable workplace ethics and ethical values for dental hygiene students, it is necessary to approach education in a way that values understanding and application of dental hygiene practices, legal and ethical standards, ethical decision-making models, and ethical principles.

19세기 博物學的 趣向과 繪畵의 새로운 경향

손정희 서울대학교 규장각한국학연구원 2008 한국문화 Vol.44 No.-

In this study, I focused on the mood of Bakhak(博學: learning in various fields) and the encyclopedic taste that emerged as new paradigm in the 18th and 19th centuries to investigate how the trend of painting in the 19th century changed. The intellectuals in that age created a new culture of reading with the books overflowing thanks to prevalence of publication culture, and the new trend based knowledge read by them changed the knowledge pattern and life style, so that such change of recognition helped creating new books based on collection & arrangement of objects also in the trend of art enjoyed by them. On this stream, a group of paintings newly emerging in the 19th century was also based on the line as the books were, which consisted of collection & arrangement of objects. This line is seen fully on the sorts of Moonbangchaekgado(文房冊架圖: painting of bookshelf) that show collection of the things for Moonbangcheongwanpoom(文房淸玩品) or on the encyclopedic paintings such as Baeknapdo(百衲圖) or Baekseondo(百扇圖) that show collection of various images about a specific object or theme. These new trend based paintings are understood as creations that show same cultural contents, which are encyclopedic interest, collection trend and combined composition shown on the encyclopedic books in the literature field, and they can be sorted as the painting image that is based on the 18th/19th century style knowledge pattern.

Hydroquinone이 인체 상피세포의 발암화에 미치는 영향

손정희,김진수,Sohn, Jung-Hee,Kim, Chin-Soo 대한악안면성형재건외과학회 2010 Maxillofacial Plastic Reconstructive Surgery Vol.32 No.3

발암물질로 알려진 Hydroquinone (HQ)은 치과용 합성수지를 구성하는 중요한 성분으로서 지금까지 치과용 재료영역에서 널리 사용되고 있으며 구강 내에서 HQ의 유출이 일어나는 것으로 확인 되었다. 따라서 구강암의 기원이 되는 인체상피세포의 발암화에 HQ가 미치는 영향을 평가하였다. HQ에 의한 인체세포 독성을 평가하기위해 LDH assay를 실시하고 세포 독성이 높지 않은 용량을 실험 용량으로 설정하였다. 인체 세포의 발암화를 평가하기 위해 세포 발암화 지표로서 cell saturation density, soft-agar colony formation 및 cell aggregation의 분석을 사용한 결과 고용량인 50 ${\mu}M$을 제외한 모든 용량에서 발암화 지표의 변화를 나타내지 않아 HQ의 발암력은 매우 낮은 것으로 추정된다. 그러나 발암촉진제인 TPA와 함께 투여 시 발암력의 증가를 보여 주변 환경의 여건에 따라 발암력이 증가할 수 있음을 입증하였다. HQ를 노출 후 세포사멸화를 측정하기 위해 DNA fragmentation변화를 분석한 결과 10 ${\mu}M$부터 50 ${\mu}M$까지 노출 시간 의존형의 증가를 나타내었으며 50 ${\mu}M$과 같은 고용량 농도에서는 노출시간 의존적 세포사멸 효과를 보였다. 따라서 세포 발암화를 일으킨 용량에서 세포사멸도 함께 일어나 HQ에 의한 발암화에 세포사멸이 관여함을 보였다. HQ는 ROS를 생성하였으며 Trolox, NAC와 같은 항산화물에 의한 ROS의 차단 효과와 BSO와 같은 GSH 고갈 유발 물질에 따른 ROS의 급격한 증가는 HQ가 인체세포에서 ROS를 효율적으로 생성함을 입증하는 결과이다. 세포간의 신호전달기작 조절에 중요한 역할을 하는 효소인 protein kinase C (PKC)를 immunoblot으로 분석한 결과 PKC-${\alpha}$의 활성이 증가 된 반면 PKC-${\beta}II$의 영향은 나타나지 않았다. 따라서 특정 이성질체에 대한 특이적인 효소반응이 발암화에 관여할 것으로 추정된다. 본 연구결과 치과용 합성수지 구성성분인 HQ 유출에 따른 인체상피세포의 발암성은 매우 낮은 것으로 추정되나 발암촉진제 등과의 상호작용에 의한 발암성 증가는 HQ의 구강암 발생 평가에 고려되어야 할 사항이다. 따라서 본 연구는 구강암의 예방을 위한 과학적인 접근 방법 및 기반 자료를 제시하였고 치과용 합성수지사용의 적정성에 대한 과학적인 판단을 할 수 있는 근거를 제공 하였다. 또한 본 연구 결과는 새로운 치과용 합성수지 개발의 필요성 및 개선방향을 제시 할 수 있는 근거로 활용될 수 있을 것으로 사료된다. Components of dental resin-based restorative materials are reported to leach from the filling materials even after polymerization. Hydroquinone (HQ) is one of the major monomers used in the dental resin and is known as a carcinogen. Thus, carcinogenic risk of HQ leaching from the dental resin becomes a public health concern. The present study attempted to examine the carcinogenic potentials of HQ on the human epithelial cell, which is the target cell origin of the most of oral cancers. Cytotoxicity of HQ was observed above 50${\mu}M$ as measured by LDH assay, indicating a relatively low toxicity of this substance in human epithelial cells. The parameters of neoplastic cellular transformation such as cell saturation density, soft agar colony formation and cell aggregation were analyzed to examine the carcinogenic potential of HQ. The study showed that 2-week exposure of HQ showed the tendency of increase in the saturation density and the significant enhancement of soft agar colony formation at the highest dose, 50 ${\mu}M$ only. It is suggested that HQ has a weak potential of carcinogenicity. When cells were treated with HQ and TPA, a well-known tumor promoter, the parameters of neoplastic cellular transformation was significantly increased. This result indicates that the potential risk of carcinogenicity from HQ is largely dependent upon the presence of promoter. Exposure of 50 ${\mu}M$ HQ increased the time-dependent apoptosis as measured by the ELISA kit. This concentration coincides with a dose of neoplastic transformation, indicating a possible link between apoptosis and HQ-induced cellular transformation. Hydroquinone generated Reactive Oxygen Species (ROS) which was evidenced by the treatment of antioxidants such as trolox and N-acetyl cysteine and the GSH depleting agent, BSO. Antioxidants blocked the generation of ROS and the GSH depleting agent, BSO dramatically increased the ROS production. Since HQ is known to increase ROS production thru activation of transcriptional factor such as c-Myb and Pim-1, it is speculated that ROS generation by HQ plays a role in the activation of oncogene, which may lead to neoplastic transformation. In addition, ROS is involved in the alteration of signal transduction, which regulates the apoptosis in many cellular systems. Thus, ROS-mediated apoptosis may be involved in the HQ-induced carcinogenic processes. Protein kinase C (PKC) is known to play pivotal roles in neoplastic transformation of cells and its high expression is often found in a variety of types of tumors including oral cancer. PKC translocation of PKC-${\alpha}$ was observed following HQ exposure. Altered signaling system may also play a role in the transformation process. Taken together, HQ leached from the dental resin does not pose a significant threat as a cancer causing agent, but its carcinogenic potential can be significantly elevated in the presence of promoter. The mechanism of HQ-induced carcinogenesis involved ROS generation, apoptosis and altered signaling pathway. The present study will provide a valuable data to estimate the potential risk of HQ as a carcinogen and understand mechanism of HQ-induced carcinogenesis in human epithelial cells.

예술가의 역할: 호손의 「미를 추구하는 예술가」와 「드라운의 목조상」을 중심으로

손정희 미국소설학회 2004 미국소설 Vol.11 No.2

Through an examination of "The Artist of the Beautiful" and "Drowne's Wooden Image," this paper aims to examine how Nathaniel Hawthorne attempted to establish authorship in an early stage of his literary career, by reading "The Artist of the Beautiful" and "Drowne's Wooden Image." Hawthorne's efforts were focused on resolving the conflict between the concept of art as a Romantic pursuit solely legitimized by the autonomy of the artist and the utilitarian and practical demands of American society in the 1840s. The two stories dramatize an artist figure who struggles between his artistic ambition and the practical demands of the society. In "The Artist of the Beautiful," Hawthorne does not celebrate the triumph of artistic achievement, which is totally isolated from social demands, by distancing himself from the protagonist, Owen Warland, who vouches for the Romantic concept of art. Meanwhile, in "Drowne's Wooden Image," Hawthorne does not portray Drowne as a failed artist with no artistic aspirations, but rather one who is merely content to lead a life of a mechanical carver. In brief, Hawthorne, in his early phase, seems to be ambivalent about the Romantic idea of authorship, balancing himself between practical needs and artistic desire. He rather attempts to find a way to "open an intercourse with the world" with a full awareness of the position by which an author situates himself as a member of the society and culture in which he lives.

19세기 碧梧社 同人들의 繪畵世界

孫禎希 미술사연구회 2003 미술사연구 Vol.- No.17