미생물 농약의 개발현황과 전망(1) -바이러스 제제를 중심으로

강석권 한국작물보호협회 1984 자연과 농업 Vol.5 No.3

미생물 농약의 개발현황과 전망 (2) - 세균 및 사상균 농약을 중심으로

강석권 한국작물보호협회 1984 자연과 농업 Vol.5 No.4

미생물 살충제의 국내실용화와 문제점

강석권 한국작물보호협회 1985 자연과 농업 Vol.6 No.8

능동 지원 규칙시스템 설계

이연식,강석권 群山大學校 情報通信技術硏究所 1998 情報通信技術硏究論文集 Vol.2 No.-

The active database system which is added conditional action functionality to the traditional database systems can support various and available functions(e.q. maintenance of integrity and security, access logging, alert, etc.) effectively. This paper presents a concept of rules which are able to support the active mechanism efficiently and designs a rule system for processing of the various rules. The proposed rule system, which is tightly coupled integrated structure, provides the efficient methods for integration and management of rules in database environment. And also, because it allow the effective processing of rules by integrations with transaction manager, data manager, query processor, and other components of database system, it contributes to the design and implementation of active database system.

솔잎 혹파리의 병원미생물에 관한 조사연구 I. 특히 가잠경화병과 관련하여

강석권,조용섭,박호용,고성철 한국잠사학회 1982 한국잠사곤충학회지 Vol.24 No.1

본시험은 솔잎혹파리의 미생물학적 방제대책의 일관으로서 미생물의 분리동정과 병원성을 연구 조사하고자 행하여졌다. 1. 솔잎혹파리의 유충에서 분리동정 된 균은 Verticillium psalliotae, Aureobdsidium pullulansa, Streptomyces sp., Aspergillus sp.(2종), Ascochyt, sp.의 6종이었으며 6종 전부가 병원성이 있었다. 2. 추기 양잠농가로부터 Spicaria pracina, Isaria farinosa, Oospora destructor, Aspergillus oryzae, A. flavus의 5종의 누에 경화병균을 분리 동정하여, 솔잎혹파리유충에 대한 병원성을 검정한 결과, 5종 전부가 강력한 병원성을 나타내었다. 3. 솔잎혹파리 유충에서는 29종의 colony가 나타났으며 그 중에서 Bacillus 계통이 11, coccus 계통이 9개로 구분되었으며, 현재 병원성검정은 진행 중에 있으나 No.4와 No.9-1균주는 병원성이 인정되었다. The study has been carried out to investigate a possibility to control the pine needle gall midge, Thecodiplosis japonensis Uchida et Inoue, by microbial pathogens as one of the microbial control measures. The samples were collected at Kocheon-Ri in the suburbs of Suweon city where were heavily damaged by this pest. Microorganisms were isolated from the both of diseased larvae and baiting method of soil microbes. In addition to, several species of the silkworm mucardine diseases were isolated for their infectivity of these fungi to the larvae of pine needle gall midge. Six species of fungi, Aureobasidium pullulans, Ascochyta sp, Verticillium psalliotae, Streptomyces sp., and two species of Aspergillus were isolated from the galls and soils, five species of muscardine diseases, Isaria farinosa, Spicaria pracina, Oospora destructor, Aspergillus flavus and A. oryzae were also identified from the silkworm corpse collected in the silkworm rearing farmers. Total of eleven species of fungi identified from the both of the larval of pine needle gall midge and silkworm larvae were tested for their pathogenecity to the larvae of pine needle gall midge. All of eleven species of fungi identified showed a considerable infectivity to the larvae. Twenty nine different kinds of bacteria were isolated from the both of diseased larvae and infested soils through the direct planting for the larvae and streaking for the corpse. The infectivity test is in progress. However two kinds of bacteria were recognized to be pathogenic to the larvae tested.

꿀벌의 질병과 방제

강석권 한국양봉협회 2005 양봉협회보 Vol.296 No.-

家蠶軟化病 및 濃核病바이러스 感染中腸組織에 있어서의 RAN 合成에 관하여

姜錫權 서울大學校 農科大學 1982 서울대농학연구지 Vol.7 No.2

RNA synthesis in the midgut of the silkworm, Bombyx mori, infected with flacherie virus (FV) was analyzed by means of the incorporation of ³H-uridine in the presence of the actinomycin D (AMD), using sucrose density gradient centrifugation. And the RNA synthesis seperated into nuclear and cytoplasmic fractions after ³H-uridine injections in the midgut infected with densonucleosis virus (DNV) of the silkworm was investigated. The virus specific RNA was synthesized in FV-infected midguts during virus mutiplication without being affected by the AMD which is the inhibitor of DNA dependent RNA polymerase, however, the cellular ribosomal RNA was severely suppressed by this chemical. This result provides an experimental basis in vivo for the characterization of the virus specific RNA synthesis in FV-infected midguts. In DNA-infected midguts,the isotope incorporation into the RNA increased markedly up to 24hr after ³H-uridine injection in both of the nuclear and cytoplasmic fractions, and after this time gradual decreasing phases of the RNA incorporation in these fractions were recognized. Accordingly,the RNA newly synthesized in DNV-infected midguts was considered to contain DNV-mRNA which was synthesized during this time as turn over point transferred into cytoplasm.

害蟲病原 바이러스의 分離同定과 바이러스 農藥 開發 : Ⅲ. Some basic studies on mass production of hyphantria cunea nucler polyhedrosis for the Ⅲ. 바이러스 製劑化를 위한 흰불나방 核多角體病 바이러스의 量産에 관한 基礎硏究

陣炳來,金英勳,金槿榮,姜錫權 서울大學校 農科大學 1985 서울대농학연구지 Vol.10 No.1

It is the intent of this research to acquire some basic informations about in vivo mass production of Hyphantria cunea muclear polyhedrosis virus for the viral pesticide. There are many factors influencing virus production through mass rearing of host larvae, but some important ones,1.e., diet for rearing, haversting time of infected larvae, and virus concentration, were examined in these expriments because those factors may play a significant role in the production system by affecting the growth of host larvae and virus yields. The 4th and 5th instar larvae of H. cunea, separately fed on artificial diet and mulberry leaves, were used for the investigation of pathogencity, comparison of changes in body weight of host larvae and virus yields on each dav after infection. 1. In the virulence of H. cunea nuclear polyherosis virus, LD?? values were 8.76x10?,1.98x10?,and 9.3x10? 5,1.5x10? PIB/ml for the 4th and 5th instar larvae of H. cunea separately fed on artificial diet and mulberry leaves, respectively. LD?? of H. cunea was 10 times higher in the larvae fed on mulberry leaves than the artificial diet. The larvae inoculated in the 5th instar were found more resistant than fourth instar to virus infection. Considerable number of larvae infected in 5th instar survived and pupated but larvae infected in the 4th instar did not survie at the concentration of 1.09x10?. 2. The body weight of H.cunea harvae infected in the 4th instar were found more reduced than the 5th instar. The increasing rate of the body weight of infected larvae fed on artificial diet was lower as compared with larvae fed on mulberry leaves. 3. The maximal virus yields of H.cunea larvae infected with 1.09x10? PIB/ml at the 5th instar were 1.3x10? PIB and 1.1x10? PIB per g of final larval weight, using mulberry leaves and artificial diet rearing, respectively. From the above results, it was demonstrated that more virus was obtained from larvae inoculated in later instars than earlier instars with sufficient concentration virus lead to 100% mortality.

한국토양으로부터 Bacillus thuringiensis 균주의 분포, 혈청학적 동정과 PCR 분석

강석권 서울대학교 농업생명과학대학 농업개발연구소 1998 농업생명과학연구 Vol.2 No.-

58 strains of Bacillus thuringiensis(B. t) were isolated from soil of various regions in Korea. Serological tests showed that B. t isolates represented 10 H serotypes, indicating a varied flora of B. t. In toxicity test, 35% of all isolated were toxic to lepidoptera, 20% were toxic to diptera, and 9% were non-toxic isolates. Especially, a large number of lepidopteran/dipteran-active isolate(36%) were found. PCR analysis using cryⅠ, Ⅱ, Ⅲ, Ⅳ and Ⅴ gene-specific primers showed that the frequency of the cryIC gene(57%) predominated, followed by the cryIA(b)(45%) and cryIIA genes(34%). But the cryIE, cryIF, cryIII, cryIVC and cryV genes were not reactive. Several isolates had unusual PCR products and multiple insecticidal crystal protein genes. PCR results showed the varied distribution of the cry-type gene.

가잠의 연화병바이러스에 관한 연구 I. 연화병바이러스의 정제

강석권 한국잠사학회 1977 한국잠사곤충학회지 Vol.19 No.1

1. 종내의 보고된 FV의 체제법을 비교검토한 결과, 어느방법도 정제도 및 정제효률에 문제가 있음을 지적하였다. 2. 종내의 정제방법을 개량하여 정제도.정제효율이 뛰어난 FV의 정제법을 확립하였다. 3. 새로운 정제법을 이나주바이러스의 정제에 적용한 결과, 본바이러스의 정제에도 유효함이 구명되었으며 동시에 최초로 본 바이러스를 정제하는데 성공하였다. 4. 새로운 정제법으로 정제한 FV 및 이나주바이러스 입자를 전자현미경으로 관찰한 결과, 이들 바이러스입자는 구형입자이고, 그 직경은 전자는 27nm, 후자는 20nm이었다. 또 이나주바이러스 입자의 침강정수는 80S였다. The flacherie (FV) and Ina-flacherie (Ina-FV, named arbitarily) viruses of the silkworm, Bombyx mori was isolated from infectious larvae. Two types of infectious particles were purified by sucrose density gradient centrifugation. Some properties of the purified particles were investigated. Electron micrographs showed that FV and Ina-FV were spherical particles with diameters of 27nm and 20nm, respectively. The sedimentation coefficient of Ina-FV was 80S.