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용매추출과 원자흡수분광법에 의한 미량 니켈의 정량에 관한 연구
강명규,정창웅 순천향대학교 기초과학연구소 2002 순천향자연과학연구 논문집 Vol.8 No.1
A selective and quantitatively atomic absorption spectrometric measurements have been developed for the determination of trace Ni(Ⅱ) with 3M-thiocyanate and 3% Aliquat 336-CCl₄. Ni(Ⅱ)-thiocyanate complex was extracted into high molecular alkylamine such as quaternary ammonium salt and stripped into 0.1M-HC1 solution. Beer's law obeys in the range of 14 ppm and concentration factor was 5X. Analysis of Ni(Ⅱ) in seawater was performed by the proposed method.
Aspergillus nidulans로부터 분획한 Protoplasts의 Reversion에 관하여
姜明奎,梁永基,李榮河 충남대학교 자연과학연구소 1984 忠南科學硏究誌 Vol.11 No.2
The processes of reversion of Aspergillus nidulans protoplasts fractionated at varing digestion periods have been investigated. The early-produced protoplasts showed higher enzyme (CMCase and β-glucosidase) activities and protein contents than the late-produced protoplasts. Similar tendencies were also observed in the reversion frequencies of the fractionated protoplasts. The reversion frequency of protoplasts isolated with 1hr-digestion was approximately 90 times higher than that of protoplasts isolated with 6hr-digestion. In liquid medium stabilized osmotically with 0.6M (NH_4)_2SO_4, two major reversion patterns of protoplasts were found. In the first, normal hyphae were directly formed from protoplasts. In the second, chains of yeast-like budding cells were produced from protoplasts and eventually normal hyphae were developed from the terminal cell of budding chain or the opposite side of the protoplasts. The proportion ratio of direct formation of normal hyphae to yeast-like forms during the processes of reversion was greatest in protoplasts isolated with 1hr-digestion and decreased in proportion to increasing digestion periods. It is suggested that the processes of reversions of A. nidulans protoplasts are related to the activities of physiological metabolism of heterogeneous protoplasts originated in highly differentiated parent mycelium.