장기간의 Pyridoxine 결핍이 새끼쥐 뇌의 신경전달 물질에 미치는 영향

지선경,최혜미 ( Sun Kyung Ji Hay Mie Choi ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.3

Weanling female Sprague Dawley rats were fed diets containing 22 ㎎ pyridoxine ·HCl/㎏(control) and 1.2 ㎎ pyridoxine ·HC1/㎏(deficient). They were fed their diets throughout growth, gestation and lactation. After the pups were born and weaned, deficient group was divided into two groups. One(DC) switched to control diet and the other(DD) continued the same deficient diet until 35 weeks of age. Body and brain weights were measured on pups at 2,3,5,7,10 and 35 weeks old. At the same intervals, offsprings were randomly chosen and decapitated. Brain were analyzed for protein, nor-epinephrine, dopamine and serotonin. And serum GOT activity were determined. Body weight of deficient group was slightly lower than the control. But brain weight was not affected by pyridoxine depletion. Especially serum GOT activity of deficient group was significantly lower than the control and brain protein also depressed in deficient groups significantly at 2nd, 3rd and 7th weeks. Brain norepinephrine level of deficient group was slightly lower than the control and brain dopamine and serotonin level of deficient group were also slightly lower than the Control.

Effect of Mild Long-term Pyridoxine Depletion on Neurotransmitters in the Developing Rat Brain

지선경,최혜미,Ji, Sun-Kyung,Choi, Hay-Mie 생화학분자생물학회 1987 한국생화학회지 Vol.20 No.3

Sprague Dawley 암쥐를 이유때부터 두군으로 나누어 각각 pyridoxine이 충분한 식이와 pyridoxine이 부족된 식이로 성장시킨 후, 적절한 시기에 임신시켜 태어난 새끼가 이유?瑛? 때 결핍식이군을 다시 두군으로 나누어 한군은 pyridoxine이 충분한 식이로 바꾸어 준 후 세군의 쥐를 35주까지 성장시켰다. 이 쥐들을 2, 3, 5, 7, 10, 35주에 임의로 몇마리씩 희생시켜서 뇌에서는 단백질, norepinephrine, dopamine, serotonin을 분석하였고 혈청에서는 GOT 활성을 측정하였다. 체중은 결핍군이 대조군보다 낮았으나 뇌의 무게는 큰차이가 없었다. 혈청 GOT 활성은 결핍군에서 대조군보다 유의적으로 낮았고 뇌의 단백질 함량도 대조군보다 결핍군에서 낮았으며 뇌의 norepinephrine, dopamine, serotonin 양은 대조군에 비하여 결핍군이 낮은 편이였다. 이 상으로써 장기간에 걸친 가벼운 정도의 pyridoxine 결핍은 새끼쥐의 뇌성장 발달에 영향을 미치는 것으로 생각되어진다. Weanling female Sprague Dawley rats were fed diets containing 22 mg pyridoxine HCl/kg(control) and 1.2 mg pyridoxine HCl/kg(deficient). They were fed their diets throughout growth, gestation and lactation. After the pups were born and weaned, deficient group was divided into two groups. One(DC) switched to control diet and the other(DD) continued the same deficient diet until 35 weeks of age. Body and brain weights were measured on pups at 2,3,5,7,10 and 35 weeks old. At the same intervals, offsprings were randomly chosen and decapitated. Brain were analyzed for protein, norepinephrine, dopamine and serotonin. And serum GOT activity were determined. Body weight of deficient group was slightly lower than the control. But brain weight was not affected by pyridoxine depletion. Especially serum GOT activity of deficient group was significantly lower than the control and brain protein also depressed in deficient groups significantly at 2nd, 3rd and 7th weeks. Brain norepinephrine level of deficient group was slightly lower than the control and brain dopamine and serotonin level of deficient group were also slightly lower than the to control.

에멀션 기반의 대형 실리카 마이크로스피어 합성에 있어서 입자표면의 균일성에 미치는 분산제의 영향성 연구

지선경,김종윤,최용석,박용준,이범재 한국공업화학회 2010 한국공업화학회 연구논문 초록집 Vol.2010 No.1

학술대회 : 장기간의 Pyridoxine 결핍이 새끼쥐 뇌의 신경전달물질에 미치는 영향

지선경,최혜미 한국영양학회 1986 총회 및 학술대회 Vol.- No.21

화학적 발암과정에서 식이의 ω6/ω3 비율이 쥐간의 전암성병변 및 지질과산화물 형성에 미치는 영향 ( Dietary ω6/ω3 ratios on the preneoplastic lesions and lipid peroxidation in diethylnitrosamine initiated rat hepatocarcinogensis )

지선경,최혜미 한국환경성돌연변이발암원학회 1996 한국환경성돌연변이·발암원학회지 Vol.16 No.2

男子大學生의 營養知識과 食習慣에 관한 調査硏究

地宣京,金賢德 기전여자대학 1991 論文集 Vol.11 No.-

The objective of this study is to investigate male college student's nutrition knowledge level and food habits and their correlation. The questionnaires answered by 378 male college students were analyzed for nutrition knowledge, food habits and their correlation. The subjects had a high level of perceived knowledge(85% ), that is the knowledge that each subject believed he had, but the accuracy of that knowledge was 71%. The mean nutrition knowledge score was 12 out of a possible 20 points, and habit score was 4.4 out of 10 points. Therefore most of the subjects had a high level of nutrition knowledge but belonged to the "Fair" food habit group, which is considered to be relatively poor. There was a Positive correlation between nutrition knowledge score and food habit score of the subjects. Furthermore with increasing monthly income and level of education of their parents, the food habit scores also increased. There was a positive correlation between nutrition knowledge score ad their mother's employment status. Meanwhile, the food habits of male college students show that the consumption level of fruits, milk and sea weed is relatively low, but vegetables consumption was normal and they ate breakfast everyday. Meals were never missed for reducing weigth. Seventy-nine percentage of the subjects have not been educated in nutrition. Most of the male students have mainly obtained nutritional information( knowledge) from mass media.

主婦들의 貯藏食品 製造 및 具備에 대한 地域間 差異 硏究(Ⅱ)

池宣京 기전여자대학 1993 論文集 Vol.13 No.-

This study was conducted in Seoul and Jeonju from May 20 to July 10, 1991, to determine the regional differences in making and keeping stored food at home. The data used in this study include 416 housewives. Frequency, percentile, mean, student's T-TEST, and oneway ANOVA were used for data analysis. The main results of this study were summarized as follows. (1) The average was 158.8㎝ in height and 56.6㎏ in weight of subjects. Obesity was more in seoul than in Jeonju. Faith of housewives were mainly Buddhism, Christianity and None. In Jeonju Christianity is main faith but in Seoul Buddhism, Sacrificial rite were more performed in Jeonju than in Seoul significantly, Most housewives used processed food for saving times primarily. In Jeonju, there was more differentiation in food distribution between son and daughter. In Seoul number of eating out were significantly higher than in Jeonju. (2) The scores of Sum1 for Seoul were higher than those of Jeonju, and these differences were sifnificant, Because the scores of Sum2, Sum3 and Sum4 for Jeonju were higher than those of Seoul, we found that housewives in Jeonju had made more stored food than in Seoul. (3) There were no regional differences in sausage, boiled fish paste, solen and jjajang, but ramyen, bread, ham, can and curry were more used in Seoul than in Jeonju. Fruits wine, dongdongju, rice cake, rice cookies, bean sprouts, bean curd, buck wheet Jelly, mung bean jelly, dried cereals, yutkireem and frying laver were more made and stored in Jeonju than in Seoul, and these differences were significant. (4) Comparing the three regions-Seoul, Jeonjusi and Jeonbuk-the scores of Sum1 showed some differences between Seoul and Jeonbuk and between Seoul and Jeonjusi, but no differences between Jeonjusi and Jeonbuk. In Sum2, Sum3 and Sum4, there were some differences between Seoul and Jeonjusi, between Seoul and Jeonbuk, and between Jeonjusi and Jeonbuk.

화학적 발암과정에서 식이의 ${\omega}6/{$\omega}3$비율이 쥐간의 전암성병변 및 지질과산화물 형성에 미치는 영향

지선경,최혜미 한국환경성돌연변이발암원학회 1996 한국환경성돌연변이·발암원학회지 Vol.16 No.2

To study the effect of dietary $\omega 6/\omega 3$ fatty acid ratios on the preneoplastic lesions and lipid peroxidation in rat hepatocellular chemical carcinogenesis, placental glutathione S-transferase(GST-P) positive foci area and numbers, glucose 6-phosphatase(G6Pase) activity, thiobarbituric acid reactive substances (TBARS) were measured. Male Sprague-Dawley rats were fed 5 different diets-low $\omega 6/\omega 3$ ratio with fish oil (Low-F), low $\omega 6/\omega 3$ ratio with perilia oil(Low-P), moderate ratio with perilia oil(Moderate), blend of 10 different commercial fats and oils(High-BL) and high $\omega 6/\omega 3$ ratio(High)-for 8 weeks. Hepatocarcinogenesis was induced by modified Ito model. The area of GST-P positive loci was the lowest in Moderate group and in ascending order of Low-F < Low-P < High-BL < High. But statistically, only Moderate and High groups were significantly different. The number of GST-P positive foci showed the same trend as foci area. The activities of G6Pase, membrane stability marker, were increased as $\omega 6/\omega 3$ ratio decreased. Lipid peroxidation values (TBARS) were the lowest in Low-F group and it is significantly different from Moderate, High-BL and High groups. When dietary $\omega 6/\omega 3$ ratio was moderate(4.06), hepatocarcinogenesis was suppressed compared with high or low $\omega 6/\omega 3$ ratios. Blend fat, commonly consumed among Koreans, did not show any suppressive effect on carcinogenesis because of high ratio(6.7). These results suggest that dietary $\omega 6/\omega 3$ ratio influences hepatocellular chemical carcinogenesis. It is recommended that appropriate $\omega 6/\omega 3$ ratio should be around 4.0. and we recommend to use more $\omega 3$ fatty acid in food preparation to reduce the risk of hepatocarcinogenesis.

메조다공성 실리카 마이크로입자: 입자합성 조건에 따른 입자 모폴로지 특성 연구

지선경,최용석,김종윤,박용준,송규석,이범재 한국공업화학회 2010 한국공업화학회 연구논문 초록집 Vol.2010 No.-

발암원을 투여한 쥐에서 식이지방의 ω6/ω3 비율이 대장의 세포증식에 미치는 영향

지선경 기전여자대학 1997 論文集 Vol.17 No.-

The study was designed to observe the effect of dietary ω6/ω3 fatty acid ratios on colon cell proliferation. Male Sprague-Dawley rats were fed 5 different diets - low ω6/ω3 ratio with fish oil (Low-F) , low ω6/ω3 ratio with perilla oil(Low-P), moderate ratio with perilla oil(Moderate), blend of 10 different commercial fats and oils(High-BL) and high ω6/ω3 ratio(High) - for 8 weeks. Crypt length of proximal and distal colon was the lowest in Low-P group, but this difference was not significant. In both proximal and distal colon crypt circumference was the lowest in Low-P group. Total cell per crypt was lowest in Low-P group in proximal colon and it is significantly different from Low-F, Moderate, High-BL and High groups. In distal colon total cells per crypt was lowest in Low-P group. Blend fat, commonly consumed among Koreans, did not show significant suppressive effect on carcinogenesis because of high ratio(6.7). Thus ω-3 α -linolenic acid rich in low ω6/ω3 ratio could have a protective effect against colon cancer compared to high ω6/ω3 ratio These results suggest that dietary ω6/ ω3 ratio influences colon cell proliferation. Therefore we recommend to use more ω3 fatty acid in food preparation to reduce the risk of colon cancer.