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      • Sclerotinia trifolorum의 Spermatizatino에 관여하는 要因의 檢索 : Ⅰ. 菌의 受精過程 Ⅰ. Course of Fertilization

        嚴在烈,金永兌 慶北大學校出版部 1987 慶北大農學誌 Vol.5 No.-

        The process of fertilization and changes in anatomical structure of sclerotia during the apothecial formation in Sclerotinia trifoliorum, the causal fungus of sclerotial rot of forage legumes, were investigated. The time of fertilization could be estimated with fair accuracy by the sequencial spermatization of the sclerotia which kept at 15C in saturated moisture. In the case of one strain used in this experiment, fertilization between the sclerotia and spermatia were estimated to take place at around 18days after the sclerotia were placed under the conditions for apothecial induction (15C, saturated moisture). The fertilizable state was maintained for about 45 days and the spermatization thereafter did not induce the apothecial formation. When the sclerotia reached fertilizable state, a number of interwoven hyphal nests were developed within the medulla of sclerotia, regardless of the sexuality of the cultures. Comparing the process of multiplication and growth of the hyphal nests in homothallic and heterothallic culture, they were identified as ascogonium. These ascognia were persisted for about 45 days. This observation was wellcoincided with the duration of fertilizable state elucidated by the sequencial spermatization experiment.

      • 사과나무 腐爛病滴 Valsa ceratosperma에서의 Auxotrophic Mutants의 檢出

        洪淵圭,嚴在烈 慶北大學校出版部 1987 慶北大農學誌 Vol.5 No.-

        This study was conducted to elucidate the most appropriate method to obtain auxotrophic mutants from Valsa ceratosperma, the causal fungus of apple canker, which may be used as a gene marker in detecting the transfer of the factors of avirulent strains to virulent strains. Among the 3 kinds of synthetic media tested, each have two formula for minimal and complete, the medium which has been used in study if Endothia parasitica (E.P medium) was turned out to be most appropriate for the growth of V. ceratosperma. A medium for single colony formation from pycnidiospore of this fungus was developed by adding 0.5% L- sorbose to the E. P minimal medium. The period of incubation in dark for preventing the photoreactivation after U. V irradiation was estimated as about 60hrs at which most of the spores become binucleate. Largest number of putative auxotrophs were obtained at about 50second of irradiation to the spores smeared on the medium for single colony formation, at which the survival rate of spores was 5 to 6 percent. With these method developed in this experiment, 161 isolates of putative auxotrophs were detected among which the nutrient requirement for 10 isolates were determind. Five out of 10 mutants were still virulent to apple tree and all but one could not sporulate.

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