放射線에 依한 水稻 可視的 突然變異의 M₃ 世代의 分離 및 劣性不足

J. L. WON(元鍾樂),S. H. KWON(權臣漢) 한국육종학회 1979 한국육종학회지 Vol.11 No.1

In order to obtain fundamental information for mutation breeding of cereal crops, segregation and recessive deficit in M₃ generation of short culm, early and late heading mutant lines were investigated in rice. About 32% of the total mutant lines selected from M₂ generation was segregated in M₃ generation, and among them segregated lines of short culm, early and late heading mutant lines were 31, 22, and 43%, respectively. Average segregation ratio in M₃ segregating lines of short culm mutants was 0.17, indicating considerable recessive deficit of mutant from. the expected ratio of 0.25, and the tendency of segregation ratio of M₃ was not in confirmity with the trend of M₂ generation. The ratio of fixed lines vs segregating lines in, M₂ basis was deviated frori the expected ratio of 1 : 2 with the deficit or excessive.

Genetic Effects of Gametophyte Irradiation in Triticale

Klu, G. Y. P.,Won, J. L. 경상대학교 유전공학연구소 1984 遺傳工學硏究所報 Vol.3 No.-

Both gametophytes at the heading stage of Triticale ev. Mizar received total exposure of 1000 R(38.8R/hr) and 1500(53R/hr) under Co-60 gamma-ray source. M_1 spike fertility was anlaysed and cytological studies were carried out on M_1 root-tip meristems and PMCs. Mo spike fertility was reduced by 33.6% at dose of 1500 R as compared to control. There was a reduced frequency of laggard chromosomes and chromosomal bridges per cell in the M_1 PMCs of irradiated spikes. The presence of ring chromosome in the PMCs of M_1 material could men a possible induction of a translocation heterozygote. Anaphase-I regularity in M_1 PMCs was less influenced in genetic point by irradiation.

水稻 突然變異系統의 세가지 形質의 遺傳硏究

S.H. KWON(權臣漢),J.L. WON(元鍾樂) 한국육종학회 1978 한국육종학회지 Vol.10 No.2

Rice mutant having brown spot color on lemma, earliness and long culmness was obtained from a population of X-irradiated Jinheung variety. To Analyse gene controlling the mutant character and to verify genetical relationships among the three characters, crossing between mutant and mother variety was made. Segregation in F₂ of each character revealed that the charactors were controlled by monogenic recessive gene and the three characters were inherited simultaneously. This implies that the mode of inheritance might be resulted by either pleiotropism or close linkage relationship of the three characters, and a definite answer will become clear by further experiments.

감마線을 處理한 大豆 第二世代에서의 突然變異出現率

S.H. KWON(權臣漢),J.L. WON(元鍾樂),H.S. SONG(宋禧燮) 한국육종학회 1981 한국육종학회지 Vol.13 No.2

In order to investigate the radiosensitivity in M₁ and frequency of mutants at seedling stage in M₂ population, the air dried seeds of three varieties were irradiated with gamma-ray doses ranged 10~50kR. The fifty percent decrease due to the radiation effects in plant height at 14 days, survival rate at 40 days after seeding, and number of pods per plant at harvest was ranged from 20 to 30 kR and their varietal differences were not found. Frequency of chlorophyll deficient mutation of leaf was 4~7% in M₁ plant bais and 2~4% in M₂ seedling basis within 20~30kR, and significant differences were not, found among the varieties. Frequency of stem color mutation in Korean native line, KAS 552-4 was higher as compared to that of chlorophyll deficient mutation and the rate was 25% and 7% in M₁ and M₂ basis, respectively. The purpose of experiment was to investigate the radiosensitivity in M₁ generation and frequency of mutants at seedling stage in M₂ population of soybean. The air dried seeds of three varieties, Chungbuk-Baik, KAS 552-4 and CB-27 were irradiated with gamma-ray doses of 10, 20, 30, 40 and 50kR. The results obtained are summarized as follows: 1. The fifty percent decrease due to the radiation effects in plant height at 14 days after seeding, survival rate at 40 days after seeding, and number of pods per plant at harvest was ranged from 20 to 30kR and their varietal differences were not found. 2. Mutant types at M₂ seedling stage were classified into chlorophyll deficiency of leaf, stem color at cotyledon stage, rosette type, twin stem, narrow leaf and multi-leaflets types. 3. Frequency of chlorophyll deficient mutation of leaf with xantha, chlorina, chlorotica and maculata type was 4~7% in M₁ plant basis and 2~4% in M₂ seedling basis within 20~30kR, and significant difference were not found among the varieties employed 4. Frequency of green stem color mutation in Korean native line, KAS 552-4 with purple stem at cotyledon stage was higher as compared to that of chlorophyll deficient mutation and the rate was about 25% and 7% in M₁ and M₂ basis, respectively.

무궁화 突然變異 育種을 위한 放射線感受性과 Chimera에 關한 硏究

S.H. KWON(權臣韓),J.L. WON(元鍾樂) 한국육종학회 1980 한국육종학회지 Vol.12 No.1

Radiosensitivity of gamma-irradiated Hibiscus syriacus and chimera formation were investigated. The lethal dose 50 percent with respect to seeding and cuttings was 15kR and 2~3kR respectively, chlorophyll mutation rate of seeds irradiated with 15kR being about 13 percent. The degree of chimeric leaf mutants from the buds by radiation treatment depends on the bud position of the branch. Buds of the middle part of V₁ branch seemed to be more multi-cellular condition than the upper and low part when irradiation was made. It is assumed that at least two primordia of V₂ branch were already differentiated from the V₁ branch in Hibiscus syriacus plant.

大豆 椄木 및 揷木法에 關한 硏究

S.H. KWON(權臣漢),J.L. WON(元鍾樂) 한국육종학회 1980 한국육종학회지 Vol.12 No.2

Grafting technique in soybean was applied in the study. Grafting was made under different growth stages using cleftgrafting method and the grafted part was binded with a thin vinyl sheet. Reciprocal graft of various varieties was fully successful at cotyledon stages. Gradual decrease of grafting percent was observed as the use of aged rootstocks and scions. Various characters, such as flower color, stem color, leaf size, seed coat color and maturity were inherited from scions. The cuttings practiced before flowering were completely successful through gravel culture method.

放射線에 의한 水稻의 蛋白質育種

C. Harn(韓昶烈),J. L. Won(元鍾樂),K. T. Choi(崔光泰) 한국육종학회 1972 한국육종학회지 Vol.4 No.2

The studies were carried out on change of protein content of high protein mutants under different cultivation conditions, correlation between protein content and agronomic characters of mutant lines, and other related works. As for the influence of sowing date, mutants behaved variously showing no definite tendency, and variation was wider than that of the mother varieties. In the mutants and their mother varieties alike, the tendency of variation of protein under different planting spaces was uniform, and the variation was not large in mutants as well as in their mother lines. Among the high protein mutant lines grown under different cultivation conditions, no correlations were found between protein percent and panicle number per unit area, between protein percent and grain size, and between grain size and protein amount per grain. In relation between protein content and some agronomic traits, there were correlations between culm length and protein, grain weight and protein content, culm length and grain weight, and between grain weight and protein per seed. High protein mutants were attributable to the corresponding increase of glutelin fraction which accounted for more than 70% of protein. Usually high protein mutant lines had lower basic amino acids per gram protein than low protein lines when estimated by DBC.

突然變異育種의 現況과 展望

S.H. KWON(權臣漢),J.L. WON(元鍾樂) 한국육종학회 1979 한국육종학회지 Vol.11 No.2

突然變異育種을 위한 推薦放射線 照射線量

S.H. KWON(權臣漢),J.L. WON(元鍾樂) 한국육종학회 1978 한국육종학회지 Vol.10 No.2

大豆의 放射線感受性과 突然變異率

S.H. KWON(權臣漢),J.L. WON(元鍾樂),J.R. KIM(金在利) 한국육종학회 1980 한국육종학회지 Vol.12 No.3

In order to obtain the basic information for the soybean mutation breeding, the seeds were treated with various gamma-ray doses of 10, 20, 30, 40, 50kR, and the response to different characters at seeding and harvest stage and mutation frequency using chlorophyll deficient mutation were investigated. Plant height, number of pods per plant and survival rate seemed to be appropriate characters to decide the radiosensitivity, and radiation doses of 20~30kR are recommended for mutation breeding. Chlorophyll deficient mutations were classified with xantha, chlorina, chlorotica and maculata types, and their frequency was 1.46 and 2.44% in M₂ plant basis at 20 and 30kR, respectively.