Double Pylorus 治驗例

元致奎,金鍾信,朴景華,尹鏞圭,崔信恩 최신의학사 1977 最新醫學 Vol.20 No.5

There have been several case reports of the double pylorus in the English literature since Christien published the first case of congenital double pylorus in 1971. A 29-year-old male who had been suffered from epigastric discomfort for about eight years turned out to have congenital double pylorus. Here we report the congenital double pylorus which was treated successfully by subtotal gastrectomy, for the first time in Korea.

실험적 간암 발생 과정에 출현하는 난원형 세포의 면역 조직화학적 연구

황택근,이중달,원치규 한양대학교 의과대학 1992 한양의대 학술지 Vol.12 No.1

Oval cells have been observed during chemical carcinogenesis in the livers of animals fed 2-acetylaminofluorene (2-AAF), a potent chemical carcinogen. Biological behavior of these cells remained poorly understood with regard to gepatic carcinogenesis. The author carried out the histological, enzyme histochemical and immunohistochemical studies of oval cells in the livers from 80 rats fed 2-AAF for five months. Throughout the experimental period oval cells along with hepatocytes and bile ductular cells were subjected to immunostain for monoclonal antibodies. The antibodies included those to carcinoembryonic antigen(CEA), alpha-fetoprotein(AFP), low molecular cytokeratin(CAM 5.2), prealbumin, and endothelial cells. Alkaline phosplatase(ALP) reaction to oval cells was also evaluated. The followings were results obtained: Oval cells appeared about the portal areas at the 10th day of the experiment. The cells penetrated the hepatic lobule as they proliferated. The proliferation of oval cells was most prominent at the 6th week and then the cells were gradually decreased in number until the late stage of the experiment. There were foci of transformation of oval cells to cholangiolar cells and foci of cholangiofibrosis made up of proliferated bile ductules. PAS reaction was negative on oval cells, where as hepatocytes were reactive for PAS. ALP reaction was positive on both oval cells and ductular epithelial cells. The hepatocytes were non-reactive for ALP. Both oval cells and bile ductular cells showed positive staining for AFP and CAM 5.2, and negative for CEA and prealbumin. Hepatocytes showed negative staining for AFP, CAM 5.2, CEA and prealbumin. However, an impressive finding that AFP- and CAM-positive hepatocytes were scattered through the hepatic lobules, was observed early during hepatocarcinogenesis. The results indicated that oval cells had no evidence of the conversion to hepatocytes in 2-AAF induced hepatocarcinogenesis, and that hepatocellular carcinoma arose not from oval cells. Oval cells showed immonohistochemical characteristics similar to those of the bile ductular epithelium.

담도계 질환자의 담즙성분의 변동에 대한 연구 - 제1보 -

원치규,이광수,김진영 대한소화기학회 1984 대한소화기학회 추계학술대회 Vol.1984 No.-

적외선 분광분석법에 의한 한국인 담석성분에 관한 연구

진광준,원치규 한양대학교 의과대학 1987 한양의대 학술지 Vol.7 No.1

Gallstones were collected from patients with cholelithiasis during laparotomy and analyzed for cholesterol, bilirubin, lecithin, palmitate and total bile acid by the method of infrared spectroscopy. In the present study, gallstones were classified with respect to absolute amount of cholesterol into 3 types; cholesterol, pigment and mixed stones. Of a total of 48 cases studied, 11(23%) were cholesterol, 18(38%) pigment and 19(39%) mixed stones. The content of cholesterol in gallstone was found to be highest in the cholesterol stone and lowest in the pigment stone. No significant differences in contents of bilirubin were observed among the 3 types of gallstones, but the highest value for ratio of content of cholesterol to bilirubin was observed in the cholesterol stone and the lowest in the pigment stone. The contents of lecithin, palmitate and total bile acid appeared to be lower in the cholesterol stone than in the pigment or mixed stone. The ratios of content of cholesterol to lecithin, palmitate and total bile acid significantly increased in the order of the pigment, mixed and cholesterol stones. These results indicated that ratios of content of cholesterol to lecithin, palmitate and total bile acid as well as content of cholesterol and ratio of content of cholesterol to bilirubin could be used as parameters in classifying and characterizing the gallstones.

骨折時 Corticosteroid 投與가 骨組織內 ^(32)p 沈着에 미치는 影響

元致奎 現代醫學社 1968 現代醫學 Vol.9 No.2

A series of experiment was conducted with male mice to observe the effect of corticosteroid n metabolism of fractured bone. One hundred and eighty mice were divided into two groups; one in vivo group and the other n vitro group. Each of these were further subdivided into control, cortisone and DOCA groups. (A) ln vivo experiment; Corticosteroid (cortisone acetate 0.5mg/day, DOCA 0. ling/day) were injected subcutaneously or three successive days and closed, complete fracture of left femur was induced manually imme-$iately following final administration of corticoid. Ten mice each were sacrificed at 2, 4 and 6 days fracture. All femurs, fracture and intact, were immersed for 24 hours in solution of the {rebs-Ringer phosphate containing two microcurie of radioactive phosphorus. (B)In vitro experiment: A complete simple fracture of left femur was incurred in each of 90 mice and these animals were sacrificed, at the various time intervals following fracture as in vivo' experiment. All femurs were incubated with the Krebs-Ringer phosphate solution (containing ZaC H332PO4) room temperature for 48 hours. The femurs of corticosteroid groups were treated with 1.5mg 'cortisone acetate and 0.3mg of DOCA. In all experiments, the colorimetric determination of phosphorus was made after the method ribed Fiske, Subbarrow and radioactivity measurement was obtained by Geiger counter. The wing results were obtained; (1) No significant changes in phosphorus level of bone were noted between fracture and acture, or between in vivo and in vitro. rvals after fracture likewise had no appreciable effect on the level of phosphorus. (2) Specific activities of the phosphorus were increased in fractured bones DOCA groups decreased in cortisone groups. It can be assumed that incorporation of bone salt is accelerated by fracture and administr) of DOCA while it is inhibited by cortisone. (3) The similar pattern was also found in specific activity of phosphorus in both vivo vitro, experiments. From the above experiment, it can be concluded that the effect of corticost on bone metabolism is direct.

결장뉴 전기활동도에 대한 Ouabain의 영향

김홍용,원치규 한양대학교 의과대학 1986 한양의대 학술지 Vol.6 No.1

The actions of ouabain on the spontaneous activity and the contractions induced by electrical stimulation were studied over the wide range of ouabain concentration in the isolated guinea-pig taenia coli. Spontaneous contractions were recorded with force transducer, and spike action potentials were measured extracelluarly by the use of suction electrode. The contractions of isolated taenia coli were induced by the electrical stimulation for 5 seconds every 1 minute with alternating current (60Hz, 3.0 V/cm) through platinum plate electrodes located in parallel with the long axis of the preparation. Effects of vanadate on spontaneous activity and electrically induced contractions were compared with those of ouabain. All experiments were performed in tris-buffered tyrode solution which was aerated with 100% O₂ and kept at 35℃. The results obtained were as follows: 1) Responses of spontaneous contractions to ouabain were dependent on the concentrations of ouabain. Almost no effect was induced by 10(???)M ouabain, and 10(???)M ouabain caused a gradual increase and maintained a certain level of tension. However, above the conentration 10(???)M ouabain an initial increase followed by a decrease in tension was observed. 2) A continuous spike discharge without a silent period was induced by the administration of 10(???)M ouabain. Above 10(???)M ouabain, a transient initial increase in electrical activity was followed by a decrease in spike frequency and amplitude and finally membrane potential was sustained at a certain level without a fluctuation and spike discharge. 3) The characteristic response to 10(???)M ouabain was not blocked by the pretreatment with 10(???)M atropine. 4) The electrically stimulated contractions were partially inhibited from the concentration of 2 x 10(???)M ouabain. As the concentration of ouabain increased, the contractions were blocked more rapidly. 5) The effects of vanadate (10(???)⁴M) were different from those of ouabain (10(???)M). Ouabain caused an initial increase followed by a decrease in tension, whereas vanadate caused a gradual increase in tension initially and then maintained a relatively constant tension. As to the contractions induced by electrical stimulation, ouabain suppressed tension development and blocked completely, while vanadate caused an initial rapid inhibition of tension development and then gradual recovery. From the above results, the following conclusions could be drawn: 1) The constant tension development induced by 10(???)M ouabain was due to continuous spike discharge without a silent period. The continuous spike discharge might be associated with a slight membrane depolarization caused by mild blockage of Na pump. 2) The biphasic response induced by above 10(???)M ouabain seemed to occur by the different mechanisms. The initial increase in tension was associated with depolarization of membrane along with an increase in spike frequency, whereas the subsequent secondary relaxation occurred through a non-electricl mechanism. 3) The characteristic response to 10(???)M ouabain was resulted not from the ouabain action on nerve terminal, but from its direct action on the membrane of smooth muscle cells.

실험적 간손상시 간 유리인자의 면역조절 작용에 대한 연구

남영수,이광수,원치규 한양대학교 의과대학 1988 한양의대 학술지 Vol.8 No.1

Despite the generally accepted knowledge that intermediate metabolic products liberated from injured hepatic cells cause serious autoimmune disease, some patient suffering from fulminating hepatitis revovers completely without proceeding to chronic hepatitis nor inducing cirrbotic changes. The fact may suggest that acutely damaged liver cells liberated both immunosuppressive factor and the factor inhibiting fibroblast proliferation which enables complete recovery by preventing sensitization to the intermediate metabolic products from injured hepatic cells and also preventing fibrotic changes of the liver tissue. In chronic damage, however, those protection liver factors decrease so that both autoimmune disease and cirrhosis are induced. A series of animal experiment using LEW mice was carried out to back up these postulation. Acute hepaic injury was induced by dimethyl nitrosamine (DMNA: 35mg/kg B.W. intravenously). Chronic injury was produced with carbon tetrachloride (CCI₄: 1ml/kg B.W. intramuscularly, b.i.w.) for 2-4 weeks and then progressed to liver cirrhosis by farther administration until 8-10 weeks. Sera and liver factors (LF) were prepared from the animals and used for inhibition tests of fibroblast proliferation and MLC reaction. Furthermore, cell-count and subpopulation of the thymus cell were done by monoclonal antibodies (W3/25, OX-8). LF of untreated (control) and DMNA-treated animals exhibited very strong unspecific inhibition effecs of fibroblast proliferation and allogenic stimulation. However, with progression of hepatic damage (chronic hepatitis and cirrhosis) both suppressive abilities gradually reduced. Normal sera showed only slight inhibition of allogenic stimulation but sera of animals with acute hepatic damage showed very strong inhibition. In the 2 weeks of CCI₄treatment, the inhibitory ability was about 40% and with progression of hepatic damage it gradually reduce. Normal sera of animals with chronic hepatic damage could not suppress the fibroblast proliferation while sera of acute hepatic damage inhibited it very strongly. With chronic hepatic damage, the thymus gradually atrophied and after 10 weeks of CCI₄treatment the atrophy was complete. Thymocytes differentiation was found only in the animals with acute hepatic damage suggesting that immunosuppressive factors liberated from the acutely damaged hepatocytes caused differentiation of the thymocytes.

1979년도 춘계 학술대회 : 59. 사체신장이식일예보고

원치규,김용일,이광수,이민혁,차희철 () 대한외과학회 1979 대한외과학회 학술대회자료집 Vol.- No.-

결장뉴 수축성에 대한 Calcium 및 Verapamil의 영향

김수길,원치규 한양대학교 의과대학 1985 한양의대 학술지 Vol.5 No.2

The influences of extracellular ?? and verapamil on the spontaneous activity and contractions induced by electrical stimulation were studied in guinea-pig taenia coli. Spontaneous contractions were recorded with force transducer, and spike action potentials were measured extracellularly by use of suction electrode. Preparations of taenia coli were stimulated for 5 seconds every 1 minute with alternating current (60Hz, 3.0 V/cm) through plate electrodes located in parallel with the long asix of the preparation. All experiments were performed in Tris-buffered Tyrode solution which was aerated with 100% O₂ and kept at 35℃. The results obtained were as follows: 1) The amplitude of spontaneous contractions (isometric tension) was maximal at the concentration of 2 mM ??, and above or below 2 mM ?? that was reduced in a dose-dependent manner. The frequency of spontaneous contractions, however, was continuously accelerated as extracellular ?? increased. Verapamil suppressed both frequency and amplitude of spontaneous contractions dose-dependently, and blocked completely at the concentration of 1 mg/1. 2) The frequency between bursts of spike discharge (bursts frequency) increased in parallel with increases in extracellular ??, whereas the frequency of spikes in a burst (spikes frequency) was maximally accelerated at the concentration of 2mM ?? and above or below 2 mM ?? that was attenuated dose-dependently. 3) Effects of ?? on the amplitude of spontaneous contractions were closely related to those on the spikes frequency, while bursts frequency determined the contractions frequency. 4) The electrically stimulated contractions were abolished in ??-free solution, and then increased dose-dependently until 4 mM ??. Above 4 mM ??, isometric contractions decreased. Verapamil depressed the electrically dirven contractions dose-dependently. 5) The inhibitory actions of verapamil on spontaneity and on electrically induced contractions were antagonized by the aministration of extra ??. The above results suggested that external ?? are essential for both excitation and contraction in guinea-pig taenia coli, and also have a stabilizing effect on the membrane, and the effects of ?? on the amplitude of spontaneous contractions result from those on spikes frequency in a burst.

제30차 학술대회 : 132. 신장이식 - 10예 분석 -

원치규,김용일,이광수,정파완,김창호 () 대한외과학회 1978 대한외과학회 학술대회자료집 Vol.- No.-