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여인호,이참결,이은영 대한약침학회 2014 Journal of pharmacopuncture Vol.17 No.1
Objectives: The objective of this study is to investigate the effects of Salviae Miltiorrhizae Radix hot aqueous extract on nitric oxide (NO) and prostaglandin E2 (PGE2) production and on 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical scavenging in macrophages. Methods: Salviae Miltiorrhizae Radix (300 g) was heated at 100℃ with distilled water (2 L) for 4 hours. The extract was filtered and concentrated to 100 mL by using a rotary evaporator, was frozen at -80℃, and was then freeze-dried by using a freezing-drying system. The RAW 264.7 macrophage was subcultured by using 10-㎍/mL lipopolysaccharide (LPS). In order to evaluate cytotoxicity, we performed (3-(4,5-dimrthylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (MTT) assays and measured the cell viability. The NO production was measured by using Griess assays, and the PGE2 production was measured by using enzyme immunoassays. The antioxidant activity, the 1,1-diphenyl-2-picryl hydrazyl (DPPH) free-radical scavenging capability, was measured by using the DPPH method. Results: Cell viability with the 1-, 5-, 25-, 125- and 625-㎍/mL Salviae Miltiorrhizae Radix hot aqueous extract was not significantly decreased compared to the cell viability without the extract. When 125 and 625 ㎍/mL of Salviae Miltiorrhizae Radix hot aqueous extract were used, nitric oxide (NO) production in LPS-stimulated RAW 264.7 macrophages was significantly inhibited compared to that in the control group. When 25, 125, and 625 ㎍/mL of Salviae Miltiorrhizae Radix hot aqueous extract were used, PGE2 production in LPS-stimulated RAW 264.7 macrophages was significantly inhibited compared to that in the control group. The 125- and 625-㎍/mL Salviae Miltiorrhizae Radix hot aqueous extracts had high DPPH free-radical scavenging capabilities in RAW 264.7 macrophages. Conclusion: This study indicates that Salviae Miltiorrhizae Radix hot aqueous extract suppresses NO and PGE2 production and improves DPPH free-radical scavenging capability. Thus, it seems that Salviae Miltiorrhizae Radix hot aqueous extract may have an anti-inflammation effect and antioxidant activity.
여인호,김정현,정세호,조나영,노정두,김갑성,이승덕,김은정,이참결,이은용 대한침구의학회 2013 대한침구의학회지 Vol.30 No.3
Objectives : The purpose of this study is to investigate the correlation between acupuncture therapy on knee osteoarthritis and BMI. Methods : Data on assessment measurements of knee osteoarthritis and obesity were obtained from 31 patients suffering from knee osteoarthritis from May, 2011 to September, 2012. The assesment measurements consisted of body mass index(BMI), visual analogue scale(VAS) and Western Ontario and McMaster Universities(WOMAC) index. Statistical correlations among assessment measurements were evaluated by examining the Pearson's correlation coefficients. Results : 1. The average age of all patients was 58.87 ± 9.48(male : 63.67 ± 11.34, female : 57.72 ± 8.86).2. The average BMI of all patients was 25.41 ± 3.06 ㎏/㎡. According to clinical definition of obesity by Korean Society for the Study of Obesity, 25.81% of all patients was within normal weight, 25.81% was overweight, 38.71% was obesity and 9.67% was morbid obesity.3. There were no significant correlations in statistics among assessment measurements. Conclusions : There is significant correlation between knee osteoarthritis and obesity according to many clinical and experimental researches. So more studies on correlation between knee osteoarthritis and obesity and development of assessment measurement are needed.