Identification of a sensitive urinary biomarker, selenium-binding protein 1, for early detection of acute kidney injury

Kim, Kyeong Seok,Yang, Hun Yong,Song, Hosup,Kang, Ye Rim,Kwon, JiHoon,An, JiHye,Son, Ji Yeon,Kwack, Seung Jun,Kim, Young-Mi,Bae, Ok-Nam,Ahn, Mee-Young,Lee, Jaewon,Yoon, Sungpil,Lee, Byung μ,Kim, Hyung TAYLOR & FRANCIS 2017 Journal of Toxicology and Environmental Health Vol.80 No.9

<P>Acute kidney injury (AKI) is associated with increased mortality rate in patients but clinically available biomarkers for disease detection are currently not available. Recently, a new biomarker, selenium-binding protein 1 (SBP1), was identified for detection of nephrotoxicity using proteomic analysis. The aim of this study was to assess the sensitivity of urinary SBP1 levels as an early detection of AKI using animal models such as cisplatin or ischemia/reperfusion (I/R). Sprague-Dawley rats were injected with cisplatin (6 mg/kg, once i.p.) and sacrificed at 1, 3, or 5 days after treatment. Ischemia was achieved by bilaterally occluding both kidneys with a microvascular clamp for 45 min and verified visually by a change in tissue color. After post-reperfusion, urine samples were collected at 9, 24, and 48 hr intervals. Urinary excretion of protein-based biomarkers was measured by Western blot analysis. In cisplatin-treated rats, mild histopathologic alterations were noted at day 1 which became severe at day 3. Blood urea nitrogen (BUN) and serum creatinine (SCr) levels were significantly increased at day 3. Levels of urinary excretion of SBP1, neutrophil gelatinase-associated lipocalin (NGAL), and a tissue inhibitor of metalloproteinase-1 (TIMP-1) were markedly elevated at day 3 and 5 following drug treatment. In the vehicle-treated I/R group, serum levels of BUN and SCr and AST activity were significantly increased compared to sham. Urinary excretion of SBP1 and NGAL rose markedly following I/R. The urinary levels of SBP1, NGAL, TIMP-1, and KIM-1 proteins excreted by AKI patients and normal subjects were compared. Among these proteins, a marked rise in SBP1 was observed in urine of patients with AKI compared to normal subjects. Based upon receiver-operator curves (ROC), SBP1 displayed a higher area under the curve (AUC) scores than levels of SCr, BUN, total protein, and glucose. In particular, SBP1 protein was readily detected in small amounts of urine without purification. Data thus indicate that urinary excretion of SBP1 may be useful as a reliable biomarker for early diagnosis of AKI in patients.</P>

남자대학생들의 피부관리실태와 관련요인 조사연구

배향선,남철현,김성우,최연희,강영숙,김선혜,구재희 慶山大學校 保健福祉硏究所 2003 保健福祉硏究 Vol.8 No.-

This study was conducted to examine aspects of skin care of male students at colleges and universities of male students at colleges and universities and its related factors in order to provide informations on skin care for men. The subjects of this study was 600 men who were students at colleges and universities in Daegu City and Kyungbuk Province. Data were collected from May 1, 2002 to June 30, 2002. The results of this study can be summarized as follows. 1. According to the respondents' socio-demographic characteristics, 55.9% of them was 20 to 24 years old and 29.6% was more than 25 years old. 84.9% of them was university students, while 15.1% was college students. 25.3% belonged to the lower class, while 22.4% belonged to the upper class. 52.7% did not have a girl friend, while 47.3% has a girl friend. 41.8% lived in big cities and 48.1% lived in small and medium cities. 2. 63.0% of the respondents replied that they were in good health, while 8.8% of them were in bad health. 41.9% of them was concerned about face and body skin care, while 14.1% was not concerned about it. Among most serious skin problems, the proportion of acne was highest (44.5%). 3. Among the persons who advised the respondents when they selected cosmetics, the proportion of selecting cosmetics as their own will was highest (44.7%). 25.3% of them selected it by recommendation of female family members and 18.3% selected it by recommendation of the girl friend. In the case of selecting it as their own will, the older they were, the higher the proportion was. In the case of having the girl friend, the proportion of selecting cosmetics by recommendation of the girl friend was higher than that of selecting it by recommendation of female family members. 4. 90.4% of the respondents replied that they never visited skin care rooms, while only 9.6% of them replied that they ever visited them. In the case of visiting skin care rooms, the proportion of the upper class was higher than that of the lower class. 5. According to the frequency of visiting skin care rooms, the proportion of once a week was highest (51.0%). 29.4% of them visited them once per 6 months and 19.6% visited them once a month. 6. In the case of the reasons they did not visit the skin care rooms, the proportion of 'no interest' was highest (36.6%). 23.2% did not visit them because they thought the fee for skin care was expensive and 19.5% did not visit them because they thought the skin care room were only for women. 7. In the variables of giving advice concerning skin care, the proportion of 'their own' was highest (29.8%). The proportion of female family members was 28.2% and the proportion of the girl friend was 27.7%. In the case of the age related to having the girl friend's advice, the proportion of 'over 25 years old' was 31.9% and the proportion of '20 years old to 24 years old' was 26.4%. The proportion of 'less than 19 years old' was 23.5%. The older they were, the more they had the girl friend's advice. 8. According to the sources of getting information on skin care, the proportion of mass media (TV, Radio etc.) was highest (36.7%). 26.0% of them obtained them through the girl friend and 13.9% obtained them through female family members. Only 8.4% got them through magazines. 9. The factors influencing the experience of visiting skin care rooms were experience of side effect of foundation cosmetics, concern about skin care, expiration date of cosmetics or checking of directions of using cosmetics, donsideration of skin character when using soap, and regular exercise for health. From above results, it can be concluded that skin care and beauty specialists working in beauty academies or the department of cosmotology at colleges or universities must make efforts to change the negative recognition of male skin care through deep and comprehensive research. It is also necessary to develop systematic education course and institutionalize the qualification management system.

악골에 생긴 조직구증-X의 임상증례 고찰

김종철,배양일,정기영 大韓顎顔面成形再建外科學會 1996 Maxillofacial Plastic Reconstructive Surgery Vol.18 No.4

Histiocytosis-X is a disorder characterized by proliferation of cells, phenotype of Langerhans cells. Traditionally, these diseases include three subtypes of commen histologic features and clinical findings respectively. Eosinophilic granuloma shows a solitary or multiple skeletal lesions. Hand-Schuller-Christian disease often fatal due to widespread extraskeletal lesions. Patient' chief compaints are frequent bone pain and swelling. During rescent 10 years in our hospital, 13 patients, 11 male and 2 female, were diagnosed as Histiocytosis-X. And we evaluated clinical features, radiographic finding, histological characteristics and treatment modalities according to the each subtypes. Major treatment methods of the eosinophic granulma was a chemotherapy combined with local currettage, wide resection of the lesion should be performed carefully in selective cases.

Ion Chromatograph에 의한 음료수중 Na^+, NH_4^+, K^+의 검출에 관한 연구

김형석,전준배,박양원 慶熙大學校 1987 論文集 Vol.16 No.-

We should drink 2-3L of the clean potable water per day to maintain our life in good condition. According to the population increase and industrialization, the water quality of the city drinking water have some problems, and the contamination of the ground water and surface water are threatening the water resources. In the analysis of the drinking water, U.S. Standard method and other several methods have been used. But recently the ion chromatography. Which could analyze anion and cation within few minutes, has developed and are using in water analysis in developed countries. Author tried to analyze monovalent cations by using single column ion chromatograph method with conductivity detector. The retention time of the Na, NH_4, and K ions are 2.62, 3.05, and 3.29 minutes under the condition of the Ion-200 Cation column(Interaction)with 10mM isonicotinic acid(pH 2.7) eluent. By using the 25ppm standard solution the relative standard deviation was within 5.0%

雨水排除 管網의 흐름 追跡 分析

金成淳,李培浩,朴浚一,李梁圭 중앙대학교 기술과학연구소 1990 기술과학연구소 논문집 Vol.20 No.-

The present study attempts to present an improved method of designing storm sewer systems by applying the flow routing through drain systems. The preformances of the new methods were tested with a reasonably acceptable result by compring the results of analyses with actual flow hydrographs absewed at the lowert points of an existing sewer system during a heavy stormrain. Comporisons between the traditional rationale method and the propased one were made with the results of analyses by two methods applied to an existing system and an experienced heady stormrain. The comparative analyses produced a generally acceptable agreement but with some discrepancies in herent to both methods. The conclusions of the present study are briefly summarized as follows : 1. The results of flow routing analyses applied to a test sewer system under a actual stormrain were in a close agreement with the observed hydrograph. 2. The comparisons of performances in an existing sewer system under an experienced stormrain were made by applying both the traditional rationale method and the flow routing method, producing a promising result. Some disagreement between the two methods were thought to be inhereut to the differences of computational procedures. 3. The comparative analyses seem to lead to a conclusion that the method based on the flow routing through the drain system reasonably reflects the physical features of the system in the computational procedures. 4. Finally, it is recommended that the traditional rational method could be applied to sewers in the earler stage of desinging storm sewers when the sized of sers are tentatively determined. The flow routing analysis may proceed with the sewer system thus determined, allowing minor modifications of sewer sizes and alignments.

感冒辨證에서 희수식 맥진기를 이용한 肺脈觀察의 臨床的 意義

김준명,고재찬,배한호,박양춘,김병탁,김철중 대전대학교 한방병원 2001 惠和醫學 Vol.10 No.1

By useing hisusik-makjingi(희수식맥진기) we analysed lung-pulse of common cold patient. The result as fellow ; 1. In case of wind-cold common cold patient, It is short-avove pulse type and when cold transfer to fever it is dry-cold type pulse. 2. In a case of wind-heat, there is a pulse of above-frequent or frequent-stringed. And also we can find out that in a mild fever case there is not a frequent pulse meaning the heat. 3. In case of vutal energy shortage and yabggi deficiency, we can also notice that the pulse is so powerless. 4. The patient who have had an old complaint and a serious illness didn't show the accordance with the pulse and common cold type.

自勵型 Brushless 單相同期發電機의 解析

尹良雄,金永炯,朴椿培 圓光大學校 1982 論文集 Vol.16 No.2

The theoretical analysis of the cylindrical magnetic fieldtyped self-excited brushless single-phase synchronous generator with inductive load was performed in this paper. The equations for the generator with inductive load was derived in considering of non-linearity character owing to the saturation of magnetic path, and the data for the design of generator was derived in this paper too. The following facts are concluded; 1) The generator characteristics with inductive load is depend upon saturation curve and the capacity of condenser. 2) The generator characteristics with inductive load has compound character as it has the shunt character by condenser currenet and the series character by load current.

접합형식구분에 따른 고강도강 기둥-보 용접접합부의 인성에 관한 연구

박양희,김성배,김종락 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.1(구조계)

This paper aimed to evaluate the characteristic and behaviour of fracture on the beam to column welded connections of high strength steel structures subjected to cyclic loading. Specimens were designed as a unit frame of tall building's middle- stores. For the study, SM570 and HP steel were used for columns while SM490 was used for beam. Ten specimens in total were carried out to the test. The major test parameters are steel strength, panel stiffness and scallop type. The results are as follows: In comparison with steel strength between SM570 steel specimen and HP steel one : HP steel steel specimen was showed over 130% of strength improvement ratio(α) and draft angle(θ) to SM 570 type according to the panel stiffness and scallop shape. Drift Angle of HP specimens and SM570 ones showed 0.071~0.091 and 0.036~0.065 radians each which partially satisfied the FEMA 350 criteria (0.04 radians for SMF). In conclusion, using weak panel and non-scallop shape specimens were considered to be superior structural strength, ductility capacity.

Global-MAHN에서 라우팅 오버헤드 개선에 대한 연구

張良根,金玄旭,李光培,吳世德,裵振勝,金在浩 明知大學校 産業技術硏究所 2007 産業技術硏究所論文集 Vol.26 No.-

In this Paper, we studied the mechanism that increases the network reliability and decreases network load in GMAHN environment based on the AODV routing protocol. This proposed algorithm is the research on the algorithm, minimizing network overhead by preventing unnecessary network overhead previously and adapting Local repair mechanism to efficient data transmission when route error happens in network, in GMAHN environment. To evaluate the proposed algorithm's performance, we analyzed the proposed algorithm and adapting in various experiment environment according to 5 experiment viewpoint such as node movement, gateway network zone, mobile node number, flow number. After evaluating the experiment, we confirmed better network improvement altogether through following result such as total average data reception rate's increase, average data transmission time's decrease, network overhead's decrease than existing GMAHN algorithm.

Factitious Fever로 밝혀진 불명열 1예

김백남,강재명,배인규,김양수,우준희,류지소 대한감염학회 2000 감염 Vol.32 No.5