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Agrobacterium Binary Vector 에 의한 포플러 형질전환을 위한 기초연구
전영우 ( Young Woo Chun,Ned B . Klopfenstein,Harold S . McNabb,Jr .,Richard B . Hall ) 한국산림과학회 1988 한국산림과학회지 Vol.77 No.2
Three clones of Populus alba × P. grandidemtata have been tested for susceptibility to Agrobacterium tumefaciens strains A281 and A348. We determined the optimum concentration of kanamycin sulfate for effective selection of leaf disc-derived, transgenic tissues transformed using Agrobacterium binary vector pGA472 containing a neomycin phosphotransferase gene (NPT-II) which confers kanamycin resistance. Of the wild type Ti plasmids contained by the two Agrobacterium strains, pTiBo542 of strain A281 appears Co be best suited to serve as a helper plasmid for binary vector systems. A relatively low concentration (10㎎/ℓ) of kanamycin sulfate inhibited adventitious shoot initiation from leaf discs on regeneration medium. Transformed kanamycin-resistant calli were obtained by culturing Agrobacterium inoculated leaf discs on selective regeneration medium. The transformed kanamycin-resistant calli continued to grow on regeneration media supplemented with kanamycin sulfate to levels of 50 and 204㎎/ℓ. The growth of non-cultivated control calli was severely inhibited on regeneration medium containing 50㎎/ℓ kanamycin sulfate.
전영우 ( Young Woo Chun,Ned B . Klopfenstein,Harold S . McNabb Jr .,Richard B . Hall ) 한국산림과학회 1988 한국산림과학회지 Vol.77 No.4
The development of tissue culture systems for Populaes species, and the utilization of tissue culture biotechnology will be reviewed and discussed. Special emphasis will be placed on prospects for genetic transformation by Agrobacterium-mediated gene transfer methods.