비육돈에 미생물제제 급여시 분뇨 특성에 미치는 효과

곽정훈,최동윤,박치호,김재환,정광화,양창범,유용희,천현식,라창식,Kwag, J.H.,Choi, D.Y.,Park, Ch.H.,Kim, J.H.,Jeong, K.H.,Yang, Ch.B.,Yoo, Y.H.,Chen, H.S.,La, C.S. 한국축산환경학회 2007 축산시설환경학회지 Vol.13 No.3

본시험은 비육돈사료에 미생물제제를 사료에 미생물제제 A 및 B 0.1 미생물제제 C를 0.2% 혼합 급여할 경우 사료섭취량 및 돈분의 오염물질 배설농도에 미치는 영향을 분석하기 위하여 4처리$\times$반복당 5두로서 총 20두를 공시하여 실시하였는데 그 결과를 요약하면 다음과 같다. 1. 비육돈의 일일 평균사료섭취량은 대조구 3.15 kg/일.두였고 미생물A, B, C구는 각각 3.14kg/일/두, 3.31, 3.42로 미생물제제 C구에서 일일 사료섭취량이 가장 높게 조사되었으며(p<0.05), 2. 일일평균 음수량은 사료섭취량이 높았던 미생물 C구에서 3.95kg/일/두로 가장 높게 조사되었다(p<0.05). 3. 미생물제제 처리구별로 분뇨배설량은 사료섭취량이 높았던 미생물제제 C구에서 가장 많이 배설되는 것으로 조사되었으며(p<0.05), 돈뇨의 배설량도 미생물제제 C구에서 2.23kg/일/두에서 높았다(p<0.05). 4. 돈분뇨의 수분 함량은 및 비료성분인 T-N, $P_{2}O_{5}$, $K_{2}O$ 성분도 처리 간에 큰 차이를 보이지 않았다(p<0.05). 5. 돈분뇨의 평균 BOD 농도는 돈분의 경우 미생물제제 B, C제제 급여구가 유의적으로 높게 조사되었다(p<0.05). 그리고 돈뇨의 BOD의 경우에는 대조구에서 $8,657.5mg/{\ell}$로 가장 높은 것으로 조사되었다(p<0.05). 6. COD 농도는 대조구에서 가장 높게 조사되었으며(p<0.05). 돈뇨의 경우에는 미생물제제 A급여구에서 평균 $9,545mg/{\ell}$로 가장 높았다(p<0.05). 7. SS 농도는 미생물제제 B급여구에서 가장 높게 조사되었으며(p<0.05), 돈분뇨중의 T-N 농도는 처리구간에 유의적인 차이가 나타나지 않았다(p<0.05). 그리고 T-P 농도의 경우에는 미생물제제 C급여구에서 유의적인 차이가 나는 것으로 조사되었다(p<0.05). 이상의 결과를 요약해보면 비육돈에 미생물제제 혼합급여시 사료섭취량과 음수량을 증가시키는데 효과가 있는 것으로 조사되었으나, 비료성분 배설량에는 큰 차이를 보이지 않는 것으로 조사되었으나, BOD 등 오염물질농도의 경우에는 미생물제제 A급여구에서 가장 낮게 조사되어 비육돈사료에 미생물제제 급여시 오염물질 저감효과가 있는 것으로 조사되었다. Study for the effect of three different microbial feed additives(henceforth MA-A, MA-B, and MA-C) on feed coversion rate, and physical and chemical characteristics of swine finisher was conducted. MA-B had higher number of Lactobacillus spp. and yeast, compared to any other. The amylase activity of MA-B was also higher than any other. The daily feed intake rates of pigs fed control, MA-A, MA-B and MA-C were 3.15, 3.14, 3.31 and 3.42 kg, respectively. MA-C had the highest weight gain. However, there was no significant difference between treatments. The weights of feces daily excreted by pigs fed control, MA-A, MA-B, and MA-C were 2.14, 2.02, 2.18, and 2.23 kg/day, respectively. The volume of urine daily excreted by pigs fed control, MA-A, MA-B, and MA-C were 3.14, 3.26, 3.27, and $3.41\;{\ell}/day$, respectively. Water content, T-N, $P_{2}O_{5}$, and $K_{2}O$ in swine manure were not significantly different between treatments. The BOD were between 42,576 and $67,450\;mg/{\ell}$ for feces and were between 5,882.5 and $8,657.5\;mg/{\ell}$ for urine, respectively. The SS were between 138,000 and $180,000\;mg/{\ell}$ for feces and were between 875.0 and $1450.0mg/{\ell}$ for urine, respectively.

Transcriptomes of major renal collecting duct cell types in mouse identified by single-cell RNA-seq

Chen, Lihe,Lee, Jae Wook,Chou, Chung-Lin,Nair, Anil V.,Battistone, Maria A.,Pă,unescu, Teodor G.,Merkulova, Maria,Breton, Sylvie,Verlander, Jill W.,Wall, Susan M.,Brown, Dennis,Burg, Maurice B. National Academy of Sciences 2017 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.114 No.46

<P><B>Significance</B></P><P>A long-term goal in mammalian biology is to identify the genes expressed in every cell type of the body. In the kidney, the expressed genes (i.e., transcriptome) of all epithelial cell types have already been identified with the exception of the cells that make up the renal collecting duct, which is responsible for regulation of blood pressure and body fluid composition. Here, single-cell RNA-sequencing was used in mouse to identify transcriptomes for the major collecting duct cell types: type A intercalated cells, type B intercalated cells, and principal cells. The information was used to create a publicly accessible online resource. The data allowed identification of genes that are selectively expressed in each cell type, which is informative for cell-level understanding of physiology and pathophysiology.</P><P>Prior RNA sequencing (RNA-seq) studies have identified complete transcriptomes for most renal epithelial cell types. The exceptions are the cell types that make up the renal collecting duct, namely intercalated cells (ICs) and principal cells (PCs), which account for only a small fraction of the kidney mass, but play critical physiological roles in the regulation of blood pressure, extracellular fluid volume, and extracellular fluid composition. To enrich these cell types, we used FACS that employed well-established lectin cell surface markers for PCs and type B ICs, as well as a newly identified cell surface marker for type A ICs, c-Kit. Single-cell RNA-seq using the IC- and PC-enriched populations as input enabled identification of complete transcriptomes of A-ICs, B-ICs, and PCs. The data were used to create a freely accessible online gene-expression database for collecting duct cells. This database allowed identification of genes that are selectively expressed in each cell type, including cell-surface receptors, transcription factors, transporters, and secreted proteins. The analysis also identified a small fraction of hybrid cells expressing aquaporin-2 and anion exchanger 1 or pendrin transcripts. In many cases, mRNAs for receptors and their ligands were identified in different cells (e.g., <I>Notch2</I> chiefly in PCs vs. <I>Jag1</I> chiefly in ICs), suggesting signaling cross-talk among the three cell types. The identified patterns of gene expression among the three types of collecting duct cells provide a foundation for understanding physiological regulation and pathophysiology in the renal collecting duct.</P>

Investigation of PCR-RFLPs within Major Histocompatibility Complex B-G Genes Using Two Restriction Enzymes in Eight Breeds of Chinese Indigenous Chickens

R. F. Xu,K. Li,G. H. Chen,B. Y. Z. Qiang,D. L. Mo,B. Fan,C. C. Li,M. Yu,M. J. Zhu,T. A. Xiong,B. Liu 아세아·태평양축산학회 2005 Animal Bioscience Vol.18 No.7

New polymorphism of major histocompatibility complex B-G genes was investigated by amplification and digestion of a 401bp fragment including intron 1 and exon 2 using polymerase chain reaction-restriction fragment length polymorphism (PCRRFLP) technique with two restriction enzymes of Msp I and Tas I in eight breeds of Chinese indigenous chickens and one exotic breed. In the fragment region of the gene, three novel single nucleotide polymorphisms (SNPs) were detected at the two restriction sites. We found the transition of two nucleotides of A294G and T295C occurred at Tas I restriction site, and consequently led to a nonsynonymous substitution of asparagine into serine at position 54 within the deduced amino acid sequence of immunoglobulin variableregion- like domain encoded by the exon 2 of B-G gene. It was observed at rare frequency that a single mutation of A294G occurring at the site, also caused an identical substitution of amino acid, asparagine 54-to-serine, to that we described previously. And the transversion of G319C at Msp I site led to a non-synonymous substitution, glutamine 62-to-histidine. The new alleles and allele frequencies identified by the PCR-RFLP method with the two enzymes were characterized, of which the allele A and B frequencies at Msp I and Tas I loci were given disequilibrium distribution either in the eight Chinese local breeds or in the exotic breed. By comparison, allele A at Msp I locus tended to be dominant, while, the allele B at Tas I locus tended to be dominant in all of the breeds analyzed. In Tibetan chickens, the preliminary association analysis revealed that no significant difference was observed between the different genotypes identified at the Msp I and Tas I loci and the laying performance traits, respectively.

Measurement of|Vub|from Inclusive Charmless SemileptonicBDecays

Urquijo, P.,Barberio, E.,Adachi, I.,Aihara, H.,Arinstein, K.,Bakich, A. M.,Belous, K.,Bhardwaj, V.,Bischofberger, M.,Bozek, A.,Brač,ko, M.,Browder, T. E.,Chao, Y.,Chen, A.,Cheon, B. G.,Chistov, R American Physical Society 2010 Physical review letters Vol.104 No.2

<P>We present the partial branching fraction for inclusive charmless semileptonic B decays and the corresponding value of the Cabibbo-Kobayashi-Maskawa matrix element vertical bar V-ub vertical bar, using a multivariate analysis method to access similar to 90% of the B -> X(u)l nu phase space. This approach dramatically reduces the theoretical uncertainties from the b-quark mass and nonperturbative QCD compared to all previous inclusive measurements. The results are based on a sample of 657 X 10(6) B (B) over bar pairs collected with the Belle detector. We find that Delta B(B -> X(u)l nu; p(l)*(B) > 1.0 GeV/c) = 1.963X(1 +/- 0.088(stat) +/- 0.081(syst)) X 10(-3). Corresponding values of vertical bar V-ub vertical bar are extracted using several theoretical calculations.</P>

CCD SNAPSHOTS OF FIELDS IN A STUDY OF THE VERTICAL DISTRIBUTION OF STARS

CHEN ALFRED B.,TSAY WEAN-SHUN,LU PHILLIP K.,SMITH ALLYN,MENDEZ RENE The Korean Astronomical Society 1996 Journal of The Korean Astronomical Society Vol.29 No.suppl1

Snapshots of eight SA and standard fields from low to high galactic latitudes were made using the KPNO 0.9m 2K $\times$ 2K CCD with a limiting magnitude from 19 to 22.5. The purpose of this study is to determine the vertical distribution of stars with respect to Galactic latitude and z-distance in comparison with the model simulation between intermediate population to the 'thick disk' component of scale height of a few kpc. Comparison of the preliminary results between observed and model simulation for 3 of the S fields shows good agreement both in V-mag and B- V color distributions. A bimodal distribution in B- V at high galactic latitude seems to be represented by a halo and 'thick disk' dwarf in the blue and by a normal disk dwarf population in the red.

Optical properties of an N-type system in Doppler-broadened multilevel atomic media of the rubidium D2 line

Chen, Yi,Wei, Xiao Gang,Ham, B S IOP Pub 2009 Journal of Physics B: Atomic, Molecular and Optica Vol.42 No.6

<P>We report the observations of Mollow sideband-like transparency windows in a Doppler-broadened four-level N-type system of the rubidium D2 line. A pair of enhanced transparency windows across an electromagnetically induced transparency line centre results from dressed state interactions of two coupling fields. The present observations have potential applications of using the symmetric transparency windows for optical quantum information processing such as multiple optical buffer memories and double slow-light-based enhanced cross-phase modulation. We also discuss the quantum coherent control of absorption cancellation, absorption enhancement and probe gain in a closed N-type four-level model introducing the atom flow rate determined by the population decay rate and control field intensity.</P>

Au-MPY/DTNBΣiO₂ SERS nanoprobe for immunosorbent assay

Chen, L.,Sa, Y.,Park, Y.,Hwang, H.,Ji, H.,Zhao, B.,Jung, Y.M. Elsevier 2016 Vibrational spectroscopy Vol.87 No.-

<P>In this paper, we developed a biocompatible surface-enhanced Raman scattering (SERS) nanoprobe that employs 4-mercaptopyridine (MPY)/5,5-dithiobis(2-nitrobenzoic acid) (DTNB)-decorated gold aggregates embedded in silicon dioxide. This highly sensitive SERS nanoprobe was applied in immunoassays. Furthermore, we constructed a sandwich (silver-proteins-Au-MPY/DTNB@SiO2) model that contributes very strong electromagnetic (EM) fields. Compared with a single layer of the SERS-active substrate, the designed model exhibited highly sensitive detection capabilities. This method demonstrates considerable potential for ultrasensitive SERS immunoassay applications. In addition, the proposed silica protected gold nanoaggregates possess good biocompatibility with proteins. (C) 2016 Elsevier B.V. All rights reserved.</P>

Large-scale dataset from China gives new insights into leaf margin-temperature relationships

Chen, Wen-Yun,Su, Tao,Adams, Jonathan M.,Jacques, Fré,dé,ric M.B.,Ferguson, David K.,Zhou, Zhe-Kun Elsevier 2014 Palaeogeography, palaeoclimatology, palaeoecology Vol.402 No.-

Leaf margin analysis (LMA) is an important method of estimating paleotemperatures from fossil leaf floras or modern floras. Although some calibration has been carried out based upon large-scale studies of modern forests, most of this research has been in North America and Europe, with relatively little calibration work in East Asia. In the present study, we used species range maps of 3116 native dicot trees of China to derive synthetic local floras for each county from the Chinese humid region, and compared the percentage of untoothed leaf margined species with several temperature and precipitation related parameters. The results confirm the generally strong relationship between the proportion of species with untoothed leaf margins and climatic parameters within China. Leaf habit (deciduous vs. evergreen) does not strongly affect this relationship. The transfer function obtained from China, while not identical, is similar to those obtained from other regions, and is affected by regional restrictions, such as complex topography and relic taxa. As such it clarifies the potential range of error inherent in the LMA method as applied to paleodimate reconstruction. It is possible, however, that with the close similarity of the modern Chinese tree floras to Neogene floras in the Northern Hemisphere, the present estimate offers a better transfer function for reconstructing the Neogene paleoclimate in various regions without extremely cold conditions across the Northern Hemisphere. (C) 2014 Published by Elsevier B.V.

Differential contribution of the mitochondrial translation pathway to the survival of diffuse large B-cell lymphoma subsets

Norberg, Erik,Lako, Ana,Chen, Pei-Hsuan,Stanley, Illana A,Zhou, Feng,Ficarro, Scott B,Chapuy, Bjoern,Chen, Linfeng,Rodig, Scott,Shin, Donghyuk,Choi, Dong Wook,Lee, Sangho,Shipp, Margaret A,Marto, Jarr Macmillan Publishers Limited, part of Springer Nat 2017 CELL DEATH AND DIFFERENTIATION Vol.24 No.2

<P>Diffuse large B-cell lymphomas (DLBCLs) are a highly heterogeneous group of tumors in which subsets share molecular features revealed by gene expression profiles and metabolic fingerprints. While B-cell receptor (BCR)-dependent DLBCLs are glycolytic, OxPhos-DLBCLs rely on mitochondrial energy transduction and nutrient utilization pathways that provide pro-survival benefits independent of BCR signaling. Integral to these metabolic distinctions is elevated mitochondrial electron transport chain (ETC) activity in OxPhos-DLBCLs compared with BCR-DLBCLs, which is linked to greater protein abundance of ETC components. To gain insights into molecular determinants of the selective increase in ETC activity and dependence on mitochondrial energy metabolism in OxPhos-DLBCLs, we examined the mitochondrial translation pathway in charge of the synthesis of mitochondrial DNA encoded ETC subunits. Quantitative mass spectrometry identified increased expression of mitochondrial translation factors in OxPhos-DLBCL as compared with the BCR subtype. Biochemical and functional assays indicate that the mitochondrial translation pathway is required for increased ETC activity and mitochondrial energy reserves in OxPhos-DLBCL. Importantly, molecular depletion of several mitochondrial translation proteins using RNA interference or pharmacological perturbation of the mitochondrial translation pathway with the FDA-approved inhibitor tigecycline (Tigecyl) is selectively toxic to OxPhos-DLBCL cell lines and primary tumors. These findings provide additional molecular insights into the metabolic characteristics of OxPhos-DLBCLs, and mark the mitochondrial translation pathway as a potential therapeutic target in these tumors.</P>

Impact of fatty liver on long-term outcomes in chronic hepatitis B: a systematic review and matched analysis of individual patient data meta-analysis

Yu Jun Wong,Vy H. Nguyen,Hwai-I Yang,Jie Li,Michael Huan Le,Wan-Jung Wu,Nicole Xinrong Han,Khi Yung Fong,Elizabeth Chen,Connie Wong,Fajuan Rui,Xiaoming Xu,Qi Xue,Xin Yu Hu,Wei Qiang Leow,George Boon-B 대한간학회 2023 Clinical and Molecular Hepatology(대한간학회지) Vol.29 No.3

Background/Aims: Chronic hepatitis B (CHB) and fatty liver (FL) often co-exist, but natural history data of this dual condition (CHB-FL) are sparse. Via a systematic review, conventional meta-analysis (MA) and individual patient-level data MA (IPDMA), we compared liver-related outcomes and mortality between CHB-FL and CHB-no FL patients. Methods: We searched 4 databases from inception to December 2021 and pooled study-level estimates using a random- effects model for conventional MA. For IPDMA, we evaluated outcomes after balancing the two study groups with inverse probability treatment weighting (IPTW) on age, sex, cirrhosis, diabetes, ALT, HBeAg, HBV DNA, and antiviral treatment. Results: We screened 2,157 articles and included 19 eligible studies (17,955 patients: 11,908 CHB-no FL; 6,047 CHB-FL) in conventional MA, which found severe heterogeneity (I2=88–95%) and no significant differences in HCC, cirrhosis, mortality, or HBsAg seroclearance incidence (P=0.27–0.93). IPDMA included 13,262 patients: 8,625 CHB-no FL and 4,637 CHB-FL patients who differed in several characteristics. The IPTW cohort included 6,955 CHB-no FL and 3,346 CHB-FL well-matched patients. CHB-FL patients (vs. CHB-no FL) had significantly lower HCC, cirrhosis, mortality and higher HBsAg seroclearance incidence (all P≤0.002), with consistent results in subgroups. CHB-FL diagnosed by liver biopsy had a higher 10-year cumulative HCC incidence than CHB-FL diagnosed with non-invasive methods (63.6% vs. 4.3%, P<0.0001). Conclusions: IPDMA data with well-matched CHB patient groups showed that FL (vs. no FL) was associated with significantly lower HCC, cirrhosis, and mortality risk and higher HBsAg seroclearance probability.