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Identification of Subspecies-specific RAPD Markers in Rice
Joong Hyoun Chin,Jung Hee Kim,Soon Wook Kwon,Young Il Cho,Zhong Ze Piao,Long Zhi Han,Hee Jong Koh 한국육종학회 2003 한국육종학회지 Vol.35 No.2
The subspecies-specif ic RAPD markers were identif ied using 30 varieties (15 japonica and 15 indica) from diverse geographical origins. Of 690 random primers tested including subspecies-specif ic (SS) RAPD markers reported earlier, 55 primers generated 6
( Yi Guang Wu ),( Zhe Jin ),( Zhong Cheng Xin ),( Wei Dong Song ),( Jing Peng ),( Zhi Chao Zhang ),( Bing Gao ),( Ze Long Kim ) 한국조직공학·재생의학회 2007 조직공학과 재생의학 Vol.4 No.4
The purpose of this experiment is to construct an in vitro model of rabbit penis enlargement using tissue engineering method, and to investigate the efficacy and safety of autologous fibroblast cell seeded to biodegradable scaffold and also to provide a new perspective of clinical application. We observed the growth of GFP transgenic fibroblast cell from SD mouse after transplantation with the biodegradable scaffold by fluorescence microscope. At the second hand, 160 male New Zealand rabbits were divided into four groups; Group A for control; Group B for acellular matrix implanted; Group C for scaffold implanted only; Group D for autologous cultured fibroblast seeded scaffold. Rabbit scrotal skin (4×4 mm) was detached under general anesthesia, and fibroblast (FB) was cultured and proliferated for 2weeks. Cultured cells were seeded into the scaffold and inserted on the between buck`s and dartos fascia of rabbit penis. To assess the efficacy and safety of the cells, we observed for 4months. The FB of rabbit were successfully cultured and confirmed by immunohistochemistry method. In the results of group D, dermal cell seeding group, the penile girth was significantly increased to 22.3% compared to other groups (Group A 0.3%, Group B 12.7%, Group C 14.6% and Group D 22.3%). We checked the results in 2~4 months later. We observed that moderate penile girth increase was gained using the biodegradable scaffold without other side-effects. Therefore, we carefully suggest a new guidance for penile augmentation by the tissue engineering method.