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Yuanyuan Fu,Jing Dong,Jianan Wang,Mingdan You,Lingling Wei,Hui Fu,Yuan Wang,Jie Chen 한국뇌신경과학회 2018 Experimental Neurobiology Vol.27 No.6
Di-(2-ethylhexyl) phthalate (DEHP) is an ubiquitous environmental contaminant because of its extensive use in plastics and its persistence. As an environmental endocrine disruptor, it is suspected to interfere with neurodevelopment in people. However, evidence of the effects of maternal DEHP exposure on cerebellar development in offspring is scarce. The objective of this study was to investigate maternal exposure to DEHP and its effect on apoptosis of cerebellar granule cells (CGCs) and related mechanisms. Pregnant Wistar rats were administrated DEHP (0, 30, 300 and 750 mg/kg/d) by gavage from gestational day (GD) 0 to postnatal day (PN) 21. Primary CGCs were also exposed to mono-(2-ethylhexyl) phthalate (MEHP), the main metabolite of DEHP, for 24 h with concentrations of 0, 25, 100 and 250 μM. The CGCs of male offspring from 300 and 750 mg/kg/d DEHP exposure groups showed significantly increased apoptosis. In addition, the PI3K/AKT signaling pathway was inhibited in the male offspring of the 300 and 750 mg/ kg/d DEHP exposure groups. However, effects on female pups were not obvious. Apoptosis was also elevated and the PI3K/AKT signaling pathway was inhibited after primary CGCs were exposed to MEHP. Furthermore, apoptosis was reduced after treatment with the PI3K/AKT signaling pathway activator, insulin-like growth factor (IGF) 1, and increased after treatment with LY294002, an inhibitor of the PI3K/AKT signaling pathway. These results suggested that maternal DEHP exposure induced apoptosis in the CGCs of male pups via the PI3K/AKT signaling pathway, and the apoptosis could be rescued by IGF1 and aggravated by LY294002.
A Rapid and Effective Colony PCR Procedure for Screening Transformants in Several Common Mushrooms
( Yuanyuan Wang ),( Danyun Xu ),( Dongmei Liu ),( Xueyan Sun ),( Yue Chen ),( Lisheng Zheng ),( Liguo Chen ),( Aimin Ma ) 한국균학회 2019 Mycobiology Vol.47 No.3
In the post-genomic era, gene function analysis has attracted much attention. Transformation is often needed to investigate gene function. In this study, an easy, rapid, reliable, and cost-effective colony polymerase chain reaction (PCR) method for screening mushroom transformants was developed: picking up a suitable amount of transformant’s tissue (1-10 lg) to 20 ll 0.25% Lywallzyme solution, and vortexing for 10 s followed by incubation at 34 ℃ for 15 min. Finally, 2 ll of the suspension was used as templates to perform PCR and single target bands were successfully amplified from respective transformants of Tremella fuciformis, Pleurotus ostreatus, and Pleurotus tuber-regium. This procedure could be widely employed for screening transformants in mushroom transformation experiments.
( Yuanyuan Wang ),( Danyun Xu ),( Xueyan Sun ),( Lisheng Zheng ),( Liguo Chen ),( Aimin Ma ) 한국균학회 2019 Mycobiology Vol.47 No.1
Agrobacterium tumefaciens-mediated transformation (ATMT), as a simple and versatile method, achieves successful transformation in the yeast-like cells (YLCs) of Tremella fuciformis with lower efficiency. Establishment of a more efficient transformation system of YLCs is important for functional genomics research and biotechnological application. In this study, an enzymolysis-assisted ATMT method was developed. The degradation degree of YLCs depends on the concentration and digestion time of Lywallzyme. Lower concentration (_0.1%) of Lywallzyme was capable of formation of limited wounds on the surface of YLCs and has less influence on their growth. In addition, there is no significant difference of YLCs growth among groups treated with 0.1% Lywallzyme for different time. The binary vector pGEH under the control of T. fuciformis glyceraldehyde-3-phosphate dehydrogenase gene (gpd) promoter was utilized to transform the enzymolytic wounded YLCs with different concentrations and digestion time. The results of PCR, Southern blot, quantitative real-time PCR (qRT-PCR) and fluorescence microscopy revealed that the T-DNA was integrated into the YLCs genome, suggesting an efficient enzymolysis-assisted ATMT method of YLCs was established. The highest transformation frequency reached 1200 transformants per 10<sup>6</sup> YLCs by 0.05% (w/v) Lywallzyme digestion for 15 min, and the transformants were genetically stable. Compared with the mechanical wounding methods, enzymolytic wounding is thought to be a tender, safer and more effective method.
중국 장애인의 교육정도가 취업여부에 미치는 영향: 사회자본 및 심리자본의 매개효과를 중심으로
WANG YUANYUAN 한국장애인복지학회 2022 한국장애인복지학 Vol.57 No.57
본 연구는 중국장애인의 교육정도가 취업여부에 영향을 미치는 데 있어서 사회자본과 심리자본의 매개효과를 검증하는 것이다. 2021년 10월 25∼11월 15일까지, 중국 난징시(南京市)내에 장애인 360명을 대상으로 설문조사를 하였다. 통계프로그램 R을 활용하여 자료를 분석하였다. 장애인의 교육정도와 취업여부의 관계에서 사회자본이 매개효과가 없고 심리자본은 부분매개효과가 있는 것을 확인하였다. 교육정도가 취업에 직접적인 영향을 미치는 것뿐만 아닌 심리자본의 매개효과를 통해 취업에 간접적인 영향도 미친다. 그리하여 장애인은 교육을 통해 지식과 기능을 얻는 것뿐만 아닌 자기효능감과 희망, 낙관적인 사고방식 등이 함께 성장함으로써 취업률을 향상하는데 큰 도움이 된다.
The Key to The Future Development of Interactive Art - Virtual Reality Technology
Wang, Junyi,Wang, Yuanyuan,Zhang, Ning,Lee, Eung-Joo,Yabin, Li,Liao, Gang Korea Multimedia Society 2018 The journal of multimedia information system Vol.5 No.4
Interactive art is an art form that closely links art creators and art participants. It has been doomed with advanced technology from the very beginning. Virtual Reality is a technology which born in the background of the maturity of computer technology. Its birth has brought immeasurable possibilities for interactive art, especially the interactivity and multi-sensory experience. This paper discusses the current development status of interactive art in the field of interactive art through the characteristics of virtual reality technology and the future development of this technology will bring to interactive art.
Wang, Yuanyuan,Chai, Shuhong,Nguyen, Hung Duc The Society of Naval Architects of Korea 2020 International Journal of Naval Architecture and Oc Vol.12 No.-
Due to the nonlinearity and environmental uncertainties, the design of the ship's steering controller is a long-term challenge. The purpose of this study is to design an intelligent autopilot based on Extended Kalman Filter (EKF) trained Radial Basis Function Neural Network (RBFNN) control algorithm. The newly developed free running model scaled surface vessel was employed to execute the motion control experiments. After describing the design of the EKF trained RBFNN autopilot, the performances of the proposed control system were investigated by conducting experiments using the physical model on lake and simulations using the corresponding mathematical model. The results demonstrate that the developed control system is feasible to be used for the ship's motion control in the presences of environmental disturbances. Moreover, in comparison with the Back-Propagation (BP) neural networks and Proportional-Derivative (PD) based control methods, the EKF RBFNN based control method shows better performance regarding course keeping and trajectory tracking.
Factors That Influence Consumer Satisfaction with Mobile Payment : The China Mobile Payment Market
Yuanyuan Wang,Joo Hwan Seo,Woon-Kyung Song 한국콘텐츠학회 2019 International Journal of Contents Vol.15 No.4
The purpose of this study was to investigate the key factors that influence consumers’ preference for mobile payment in China. China has been quietly experiencing a third technological revolution that has markedly changed the way of life for its people. We used the structural equation modeling with 573 Chinese people to investigate the mobile-payment system in China based on the technology acceptance model. We found that factors such as value of service, security, convenience, and perceived usefulness have an impact on consumer satisfaction, and that satisfaction supports consumer purchasing. Also, it is possible to conclude that this proven instrument will assist researchers to further develop and refine mobile-payment research models.
A Novel Strategy for the Microbial Removal of Heavy Metals: Cell-surface Display of Peptides
Yuanyuan Wang,Vidhya Selvamani,유익근,김태완,홍순호 한국생물공학회 2021 Biotechnology and Bioprocess Engineering Vol.26 No.1
Cell surface engineering is a rapidly developing technology of microorganism that achieves modification of cell surface function by joining external functional peptides with surface anchoring proteins, for example, Outer Member Protein (OMP). On account of these proteins possessing metal responsive motifs, they can be specifically used for metal adsorption and dissociation. To elucidate the problems caused by heavy metals and develop various technologies for their removel or recovery, various metalbinding proteins/peptides fused on microorganism cell surface have been applied as novel methods. During the past few years, bacterial cell surface display strategy has received growing attention for their availability to eliminate heavy metals. In this paper, the existing problems, progress, and suggestions for furture of the peptide displaying system are summarized.